csrA

Summary

Gene Symbol: csrA
Description: pleiotropic regulatory protein for carbon source metabolism
Alias: ECK2691, JW2666, zfiA
Species: Escherichia coli str. K-12 substr. MG1655
Products:     csrA

Top Publications

  1. Gudapaty S, Suzuki K, Wang X, Babitzke P, Romeo T. Regulatory interactions of Csr components: the RNA binding protein CsrA activates csrB transcription in Escherichia coli. J Bacteriol. 2001;183:6017-27 pubmed publisher
    The global regulator CsrA (carbon storage regulator) of Escherichia coli is a small RNA binding protein that represses various metabolic pathways and processes that are induced in the stationary phase of growth, while it activates certain ..
  2. Wei B, Brun Zinkernagel A, Simecka J, Prüss B, Babitzke P, Romeo T. Positive regulation of motility and flhDC expression by the RNA-binding protein CsrA of Escherichia coli. Mol Microbiol. 2001;40:245-56 pubmed
    ..We now show that the global regulator CsrA of E...
  3. Jonas K, Edwards A, Simm R, Romeo T, Romling U, Melefors O. The RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins. Mol Microbiol. 2008;70:236-57 pubmed publisher
    The carbon storage regulator CsrA is an RNA binding protein that controls carbon metabolism, biofilm formation and motility in various eubacteria...
  4. Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006;281:31832-42 pubmed
    The RNA-binding protein CsrA (carbon storage regulator) of Escherichia coli is a global regulator of gene expression and is representative of the CsrA/RsmA family of bacterial proteins...
  5. Liu M, Yang H, Romeo T. The product of the pleiotropic Escherichia coli gene csrA modulates glycogen biosynthesis via effects on mRNA stability. J Bacteriol. 1995;177:2663-72 pubmed
    The carbon storage regulator gene, csrA, modulates the expression of genes in the glycogen biosynthesis and gluconeogenesis pathways in Escherichia coli and has been cloned, mapped and sequenced (T. Romeo, M. Gong, M.Y. Liu, and A.M...
  6. Romeo T, Gong M, Liu M, Brun Zinkernagel A. Identification and molecular characterization of csrA, a pleiotropic gene from Escherichia coli that affects glycogen biosynthesis, gluconeogenesis, cell size, and surface properties. J Bacteriol. 1993;175:4744-55 pubmed
    ..The mutated gene was designated csrA for carbon storage regulator...
  7. Jackson D, Suzuki K, Oakford L, Simecka J, Hart M, Romeo T. Biofilm formation and dispersal under the influence of the global regulator CsrA of Escherichia coli. J Bacteriol. 2002;184:290-301 pubmed
    ..Here we establish that the RNA binding global regulatory protein CsrA (carbon storage regulator) of Escherichia coli K-12 serves as both a repressor of biofilm formation and an ..
  8. Edwards A, Patterson Fortin L, Vakulskas C, Mercante J, Potrykus K, Vinella D, et al. Circuitry linking the Csr and stringent response global regulatory systems. Mol Microbiol. 2011;80:1561-80 pubmed publisher
    b>CsrA protein regulates important cellular processes by binding to target mRNAs and altering their translation and/or stability. In Escherichia coli, CsrA binds to sRNAs, CsrB and CsrC, which sequester CsrA and antagonize its activity...
  9. Timmermans J, Van Melderen L. Conditional essentiality of the csrA gene in Escherichia coli. J Bacteriol. 2009;191:1722-4 pubmed publisher
    b>CsrA is a global posttranscriptional regulator of numerous physiological processes, such as glycogenesis and glycolysis...

More Information

Publications61

  1. Weilbacher T, Suzuki K, Dubey A, Wang X, Gudapaty S, Morozov I, et al. A novel sRNA component of the carbon storage regulatory system of Escherichia coli. Mol Microbiol. 2003;48:657-70 pubmed
    ..CsrC RNA binds multiple copies of CsrA, a protein that post-transcriptionally regulates central carbon flux, biofilm formation and motility in E. coli...
  2. Suzuki K, Wang X, Weilbacher T, Pernestig A, Melefors O, Georgellis D, et al. Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli. J Bacteriol. 2002;184:5130-40 pubmed
    The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli...
  3. Wang X, Dubey A, Suzuki K, Baker C, Babitzke P, Romeo T. CsrA post-transcriptionally represses pgaABCD, responsible for synthesis of a biofilm polysaccharide adhesin of Escherichia coli. Mol Microbiol. 2005;56:1648-63 pubmed
    The RNA-binding protein CsrA represses biofilm formation, while the non-coding RNAs CsrB and CsrC activate this process by sequestering CsrA...
  4. Altier C, Suyemoto M, Lawhon S. Regulation of Salmonella enterica serovar typhimurium invasion genes by csrA. Infect Immun. 2000;68:6790-7 pubmed
    ..We report here that epithelial cell invasion requires the serovar Typhimurium homologue of Escherichia coli csrA, which encodes a regulator that alters the stability of specific mRNA targets...
  5. Romeo T. Global regulation by the small RNA-binding protein CsrA and the non-coding RNA molecule CsrB. Mol Microbiol. 1998;29:1321-30 pubmed
    ..Its effector is a small RNA-binding protein referred to as CsrA or, in phytopathogenic Erwinia species, RsmA (repressor of stationary phase metabolites)...
  6. Liu M, Gui G, Wei B, Preston J, Oakford L, Yuksel U, et al. The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli. J Biol Chem. 1997;272:17502-10 pubmed
    The RNA-binding protein CsrA (carbon storage regulator) is a new kind of global regulator, which facilitates specific mRNA decay...
  7. Mercante J, Edwards A, Dubey A, Babitzke P, Romeo T. Molecular geometry of CsrA (RsmA) binding to RNA and its implications for regulated expression. J Mol Biol. 2009;392:511-28 pubmed publisher
    The global regulatory protein CsrA binds to the 5'-untranslated leader of target transcripts and alters their translation and/or stability. CsrA is a symmetrical homodimer containing two identical RNA-binding surfaces...
  8. Yakhnin H, Yakhnin A, Baker C, Sineva E, Berezin I, Romeo T, et al. Complex regulation of the global regulatory gene csrA: CsrA-mediated translational repression, transcription from five promoters by E??? and E?(S), and indirect transcriptional activation by CsrA. Mol Microbiol. 2011;81:689-704 pubmed publisher
    b>CsrA of Escherichia coli is an RNA-binding protein that globally regulates gene expression by repressing translation and/or altering the stability of target transcripts. Here we explored mechanisms that control csrA expression...
  9. Yakhnin H, Baker C, Berezin I, Evangelista M, Rassin A, Romeo T, et al. CsrA represses translation of sdiA, which encodes the N-acylhomoserine-L-lactone receptor of Escherichia coli, by binding exclusively within the coding region of sdiA mRNA. J Bacteriol. 2011;193:6162-70 pubmed publisher
    The RNA binding protein CsrA is the central component of a conserved global regulatory system that activates or represses gene expression posttranscriptionally...
  10. Baker C, Morozov I, Suzuki K, Romeo T, Babitzke P. CsrA regulates glycogen biosynthesis by preventing translation of glgC in Escherichia coli. Mol Microbiol. 2002;44:1599-610 pubmed
    ..b>CsrA binding to glgCAP transcripts inhibits glycogen metabolism by promoting glgCAP mRNA decay...
  11. Yakhnin A, Baker C, Vakulskas C, Yakhnin H, Berezin I, Romeo T, et al. CsrA activates flhDC expression by protecting flhDC mRNA from RNase E-mediated cleavage. Mol Microbiol. 2013;87:851-66 pubmed publisher
    Csr is a conserved global regulatory system that controls expression of several hundred Escherichia coli genes. CsrA protein represses translation of numerous genes by binding to mRNA and inhibiting ribosome access...
  12. Gutierrez P, Li Y, Osborne M, Pomerantseva E, Liu Q, Gehring K. Solution structure of the carbon storage regulator protein CsrA from Escherichia coli. J Bacteriol. 2005;187:3496-501 pubmed
    The carbon storage regulator A (CsrA) is a protein responsible for the repression of a variety of stationary-phase genes in bacteria...
  13. Baker C, Eöry L, Yakhnin H, Mercante J, Romeo T, Babitzke P. CsrA inhibits translation initiation of Escherichia coli hfq by binding to a single site overlapping the Shine-Dalgarno sequence. J Bacteriol. 2007;189:5472-81 pubmed
    Csr (carbon storage regulation) of Escherichia coli is a global regulatory system that consists of CsrA, a homodimeric RNA binding protein, two noncoding small RNAs (sRNAs; CsrB and CsrC) that function as CsrA antagonists by sequestering ..
  14. Heeb S, Kuehne S, Bycroft M, Crivii S, Allen M, Haas D, et al. Functional analysis of the post-transcriptional regulator RsmA reveals a novel RNA-binding site. J Mol Biol. 2006;355:1026-36 pubmed
    ..Thus, although possessing a motif similar to the KH domain of some eukaryotic RNA-binding proteins, RsmA differs substantially and incorporates a novel class of RNA-binding site. ..
  15. Dubey A, Baker C, Romeo T, Babitzke P. RNA sequence and secondary structure participate in high-affinity CsrA-RNA interaction. RNA. 2005;11:1579-87 pubmed
    The global Csr regulatory system controls bacterial gene expression post-transcriptionally. CsrA of Escherichia coli is an RNA binding protein that plays a central role in repressing several stationary phase processes and activating ..
  16. Dubey A, Baker C, Suzuki K, Jones A, Pandit P, Romeo T, et al. CsrA regulates translation of the Escherichia coli carbon starvation gene, cstA, by blocking ribosome access to the cstA transcript. J Bacteriol. 2003;185:4450-60 pubmed
    b>CsrA is a global regulator that binds to two sites in the glgCAP leader transcript, thereby blocking ribosome access to the glgC Shine-Dalgarno sequence...
  17. Cui Y, Chatterjee A, Liu Y, Dumenyo C, Chatterjee A. Identification of a global repressor gene, rsmA, of Erwinia carotovora subsp. carotovora that controls extracellular enzymes, N-(3-oxohexanoyl)-L-homoserine lactone, and pathogenicity in soft-rotting Erwinia spp. J Bacteriol. 1995;177:5108-15 pubmed
    ..8-kDa RsmA. rsmA has extensive homology with the csrA gene of Escherichia coli, which specifies a negative regulator of carbon storage...
  18. Sabnis N, Yang H, Romeo T. Pleiotropic regulation of central carbohydrate metabolism in Escherichia coli via the gene csrA. J Biol Chem. 1995;270:29096-104 pubmed
    The carbon storage regulator gene csrA has been shown previously to dramatically affect the biosynthesis of intracellular glycogen in Escherichia coli through its negative control of the expression of two glycogen biosynthetic operons and ..
  19. Wei B, Shin S, Laporte D, Wolfe A, Romeo T. Global regulatory mutations in csrA and rpoS cause severe central carbon stress in Escherichia coli in the presence of acetate. J Bacteriol. 2000;182:1632-40 pubmed
    The csrA gene encodes a small RNA-binding protein, which acts as a global regulator in Escherichia coli and other bacteria (T. Romeo, Mol. Microbiol. 29:1321-1330, 1998)...
  20. Suzuki K, Babitzke P, Kushner S, Romeo T. Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E. Genes Dev. 2006;20:2605-17 pubmed
    In Escherichia coli, the global regulatory protein CsrA (carbon store regulator A) binds to leader segments of target mRNAs, affecting their translation and stability...
  21. Tatarko M, Romeo T. Disruption of a global regulatory gene to enhance central carbon flux into phenylalanine biosynthesis in Escherichia coli. Curr Microbiol. 2001;43:26-32 pubmed
    ..Disruption of csrA increases gluconeogenesis, decreases glycolysis, and thus elevates phosphoenolpyruvate, a limiting precursor of ..
  22. Yang T, Sung Y, Lei G, Romeo T, Chak K. Posttranscriptional repression of the cel gene of the ColE7 operon by the RNA-binding protein CsrA of Escherichia coli. Nucleic Acids Res. 2010;38:3936-51 pubmed publisher
    Carbon storage regulator (CsrA) is a eubacterial RNA-binding protein that acts as a global regulator of many functionally diverse chromosomal genes...
  23. Ricci V, Attah V, Overton T, Grainger D, Piddock L. CsrA maximizes expression of the AcrAB multidrug resistance transporter. Nucleic Acids Res. 2017;45:12798-12807 pubmed publisher
    Carbon Storage Regulator A (CsrA) is an RNA binding protein that acts as a global regulator of diverse genes. Using a combination of genetics and biochemistry we show that CsrA binds directly to the 5' end of the transcript encoding AcrAB...
  24. Esquerré T, Bouvier M, Turlan C, Carpousis A, Girbal L, Cocaign Bousquet M. The Csr system regulates genome-wide mRNA stability and transcription and thus gene expression in Escherichia coli. Sci Rep. 2016;6:25057 pubmed publisher
    ..Two proteins, an RNA-binding protein CsrA and an atypical signaling protein CsrD, participate in the Csr system...
  25. Adamson D, Lim H. Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013;110:13120-5 pubmed publisher
    ..This study systematically characterizes the signaling dynamics of a cascade of RNA-protein interactions in the CsrA system, which regulates stress responses and biofilm formation in Escherichia coli...
  26. Pannuri A, Yakhnin H, Vakulskas C, Edwards A, Babitzke P, Romeo T. Translational repression of NhaR, a novel pathway for multi-tier regulation of biofilm circuitry by CsrA. J Bacteriol. 2012;194:79-89 pubmed publisher
    The RNA binding protein CsrA (RsmA) represses biofilm formation in several proteobacterial species...
  27. Murayama N, Shimizu H, Takiguchi S, Baba Y, Amino H, Horiuchi T, et al. Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F. J Mol Biol. 1996;256:483-502 pubmed
    ..coli genes tldD, tldE and zfiA participate in the process, in addition to the groE genes we reported previously...
  28. Heeb S, Haas D. Regulatory roles of the GacS/GacA two-component system in plant-associated and other gram-negative bacteria. Mol Plant Microbe Interact. 2001;14:1351-63 pubmed publisher
    ..expression is regulated by the GacS/GacA system are regulated in parallel by the small RNA binding protein RsmA (CsrA) at a posttranscriptional level...
  29. Hardie K, Cooksley C, Green A, Winzer K. Autoinducer 2 activity in Escherichia coli culture supernatants can be actively reduced despite maintenance of an active synthase, LuxS. Microbiology. 2003;149:715-28 pubmed publisher
    ..repression (creC, fruR, rpoS,) the stringent response (relA, spoT) and the global carbon storage regulator (csrA) did not prevent GRAIL...
  30. Murray E, Conway T. Multiple regulators control expression of the Entner-Doudoroff aldolase (Eda) of Escherichia coli. J Bacteriol. 2005;187:991-1000 pubmed
    ..P4 is active under conditions of phosphate starvation and is directly controlled by PhoB. In addition, CsrA activates Eda synthesis, apparently by an indirect mechanism that may be involved in the modest changes in ..
  31. Camacho M, Alvarez A, Chavez R, Romeo T, Merino E, Georgellis D. Effects of the global regulator CsrA on the BarA/UvrY two-component signaling system. J Bacteriol. 2015;197:983-91 pubmed publisher
    ..These two small RNAs act by sequestering the RNA binding protein CsrA, which posttranscriptionally regulates translation and/or stability of its target mRNAs...
  32. Jin Y, Watt R, Danchin A, Huang J. Use of a riboswitch-controlled conditional hypomorphic mutation to uncover a role for the essential csrA gene in bacterial autoaggregation. J Biol Chem. 2009;284:28738-45 pubmed publisher
    ..We demonstrate the utility of this approach with csrA, an essential gene in Escherichia coli that encodes the global regulatory protein CsrA...
  33. Campbell T, Brown E. Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis. J Bacteriol. 2008;190:2537-45 pubmed publisher
    ..Taken in context, our work is most consistent with the hypothesis that YjeQ has a role in late 30S subunit biogenesis. ..
  34. Pannuri A, Vakulskas C, Zere T, McGibbon L, Edwards A, Georgellis D, et al. Circuitry Linking the Catabolite Repression and Csr Global Regulatory Systems of Escherichia coli. J Bacteriol. 2016;198:3000-3015 pubmed
    Cyclic AMP (cAMP) and the cAMP receptor protein (cAMP-CRP) and CsrA are the principal regulators of the catabolite repression and carbon storage global regulatory systems, respectively...
  35. Pernestig A, Georgellis D, Romeo T, Suzuki K, Tomenius H, Normark S, et al. The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources. J Bacteriol. 2003;185:843-53 pubmed
    ..Results from competitions with mutants in the carbon storage regulation system, CsrA/B, known to be a master switch between glycolysis and gluconeogenesis, led us to propose that the BarA-UvrY two-..
  36. Nogueira T, Springer M. Post-transcriptional control by global regulators of gene expression in bacteria. Curr Opin Microbiol. 2000;3:154-8 pubmed
    ..case for Hfq (HF-1), which is involved in the regulation of an increasing number of genes in Escherichia coli, and CsrA (RsmA) responsible for controlling the expression of genes for extracellular enzymes and secondary metabolism in ..
  37. Liu M, Romeo T. The global regulator CsrA of Escherichia coli is a specific mRNA-binding protein. J Bacteriol. 1997;179:4639-42 pubmed
    The csrA gene encodes a global regulatory protein which facilitates glgC mRNA decay in vivo...
  38. Hamilton H, Wilson R, Blythe M, Nehring R, Fonville N, Louis E, et al. Thymineless death is inhibited by CsrA in Escherichia coli lacking the SOS response. DNA Repair (Amst). 2013;12:993-9 pubmed publisher
    ..One of the additional mutations, csrA, confers TLD sensitivity specifically in SOS-defective strains...
  39. Morin M, Ropers D, Letisse F, Laguerre S, Portais J, Cocaign Bousquet M, et al. The post-transcriptional regulatory system CSR controls the balance of metabolic pools in upper glycolysis of Escherichia coli. Mol Microbiol. 2016;100:686-700 pubmed publisher
    ..The post-transcriptional factor CsrA is stated as being the only regulator essential for the use of glycolytic substrates...
  40. Agladze K, Wang X, Romeo T. Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA. J Bacteriol. 2005;187:8237-46 pubmed
    ..A csrA mutant, which forms biofilm more vigorously than its wild-type parent, exhibited an increased proportion of ..
  41. Kulkarni P, Cui X, Williams J, Stevens A, Kulkarni R. Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri. Nucleic Acids Res. 2006;34:3361-9 pubmed
    ..small RNAs is represented by CsrB and CsrC of Escherichia coli, which control the activity of the global regulator CsrA. Given the critical role played by CsrA in several bacterial species, an important problem is the identification of ..
  42. Jiang G, Nikolova S, Clark D. Regulation of the ldhA gene, encoding the fermentative lactate dehydrogenase of Escherichia coli. Microbiology. 2001;147:2437-46 pubmed publisher
    ..Disruption of csrA caused a moderate drop in expression of both operon and protein ldhA fusions, whereas insertional inactivation of ..
  43. Seyll E, Van Melderen L. The ribonucleoprotein Csr network. Int J Mol Sci. 2013;14:22117-31 pubmed publisher
    ..This regulatory network is composed of the CsrA master regulator, its targets and regulators...
  44. Vakulskas C, Leng Y, Abe H, Amaki T, Okayama A, Babitzke P, et al. Antagonistic control of the turnover pathway for the global regulatory sRNA CsrB by the CsrA and CsrD proteins. Nucleic Acids Res. 2016;44:7896-910 pubmed publisher
    The widely conserved protein CsrA (carbon storage regulator A) globally regulates bacterial gene expression at the post-transcriptional level...
  45. Cerca N, Jefferson K. Effect of growth conditions on poly-N-acetylglucosamine expression and biofilm formation in Escherichia coli. FEMS Microbiol Lett. 2008;283:36-41 pubmed publisher
    ..In this paper we investigated pga expression in wild type and csrA or nhaR deletion mutant strains during different growth phases and temperatures, and in response to chemical ..
  46. Chang H, Yang T, Lei G, Chak K. A novel endogenous induction of ColE7 expression in a csrA mutant of Escherichia coli. Curr Microbiol. 2013;66:392-7 pubmed publisher
    Carbon storage regulator A (CsrA) is an important regulator that controls central metabolic pathways and a variety of physiological functions...
  47. Bhatt S, Anyanful A, Kalman D. CsrA and TnaB coregulate tryptophanase activity to promote exotoxin-induced killing of Caenorhabditis elegans by enteropathogenic Escherichia coli. J Bacteriol. 2011;193:4516-22 pubmed publisher
    ..Here, we demonstrate that the RNA-binding protein CsrA and the tryptophan permease TnaB coregulate tryptophanase activity, through mutually exclusive pathways, to ..
  48. White D, Hart M, Romeo T. Phylogenetic distribution of the global regulatory gene csrA among eubacteria. Gene. 1996;182:221-3 pubmed
    The gene csrA encodes a unique kind of global regulator, CsrA, which modulates glycolysis, gluconeogenesis, glycogen biosynthesis and glycogen catabolism in Escherichia coli...
  49. Park H, McGibbon L, Potts A, Yakhnin H, Romeo T, Babitzke P. Translational Repression of the RpoS Antiadapter IraD by CsrA Is Mediated via Translational Coupling to a Short Upstream Open Reading Frame. MBio. 2017;8: pubmed publisher
    b>CsrA is a global regulatory RNA binding protein that has important roles in regulating carbon metabolism, motility, biofilm formation, and numerous other cellular processes...
  50. Romeo T. Post-transcriptional regulation of bacterial carbohydrate metabolism: evidence that the gene product CsrA is a global mRNA decay factor. Res Microbiol. 1996;147:505-12 pubmed
  51. Romeo T, Gong M. Genetic and physical mapping of the regulatory gene csrA on the Escherichia coli K-12 chromosome. J Bacteriol. 1993;175:5740-1 pubmed
  52. Yang H, Liu M, Romeo T. Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product. J Bacteriol. 1996;178:1012-7 pubmed
    The carbon storage regulator gene, csrA, encodes a factor which negatively modulates the expression of the glycogen biosynthetic gene glgC by enhancing the decay of its mRNA (M. Y. Liu, H. Yang, and T. Romeo, J. Bacteriol...