Gene Symbol: cspB
Description: Qin prophage; cold shock protein
Alias: ECK1551, JW1549
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Phadtare S, Inouye M. Sequence-selective interactions with RNA by CspB, CspC and CspE, members of the CspA family of Escherichia coli. Mol Microbiol. 1999;33:1004-14 pubmed
    ..a step towards identification of the target genes of Csp proteins, we investigated the RNA binding specificities of CspB, CspC and CspE by an in vitro selection approach (SELEX)...
  2. Xia B, Ke H, Inouye M. Acquirement of cold sensitivity by quadruple deletion of the cspA family and its suppression by PNPase S1 domain in Escherichia coli. Mol Microbiol. 2001;40:179-88 pubmed
    ..of two million molecules per cell at low temperature and growth defect was not observed until four csp genes (cspA, cspB, cspE and cspG) were deleted...
  3. Nakaminami K, Karlson D, Imai R. Functional conservation of cold shock domains in bacteria and higher plants. Proc Natl Acad Sci U S A. 2006;103:10122-7 pubmed
    ..coli CSPs. Constitutive expression of WT WCSP1 in an E. coli cspA, cspB, cspE, cspG quadruple deletion mutant complemented its cold-sensitive phenotype, suggesting that WCSP1 shares a ..
  4. Lee S, Xie A, Jiang W, Etchegaray J, Jones P, Inouye M. Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins. Mol Microbiol. 1994;11:833-9 pubmed
    ..DNA sequencing of these clones confirmed that the two genes are highly homologous to cspA. One designated cspB is mapped at 35 min on the E...
  5. Bae W, Jones P, Inouye M. CspA, the major cold shock protein of Escherichia coli, negatively regulates its own gene expression. J Bacteriol. 1997;179:7081-8 pubmed
    ..The production of the CspA homologs CspB and CspG increased, and the duration of their expression was prolonged, suggesting that both CspB and CspG ..
  6. Etchegaray J, Jones P, Inouye M. Differential thermoregulation of two highly homologous cold-shock genes, cspA and cspB, of Escherichia coli. Genes Cells. 1996;1:171-8 pubmed
    ..4 kDa protein. A CspA family has been found which consists of four additional proteins, CspB, CspC, CspD and CspE. The expression of cspB, unlike the other homologues, is cold-shock inducible like cspA...
  7. Etchegaray J, Inouye M. CspA, CspB, and CspG, major cold shock proteins of Escherichia coli, are induced at low temperature under conditions that completely block protein synthesis. J Bacteriol. 1999;181:1827-30 pubmed
    CspA, CspB, and CspG, the major cold shock proteins of Escherichia coli, are dramatically induced upon temperature downshift...
  8. Etchegaray J, Inouye M. A sequence downstream of the initiation codon is essential for cold shock induction of cspB of Escherichia coli. J Bacteriol. 1999;181:5852-4 pubmed
    Cold shock induction of cspB has been shown to be primarily regulated at the mRNA level. Here, we demonstrate that the induction of cspB at low temperature also requires the translational cis-acting element called the downstream box (DB)...
  9. Kim J, Park S, Kwak K, Kim Y, Kim J, Song J, et al. Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote the cold adaptation process in Escherichia coli. Nucleic Acids Res. 2007;35:506-16 pubmed
    ..Together, these results indicate that CSDPs and GRPs help E.coli grow and survive better during cold shock, and strongly imply that CSDP1 and GRP7 exhibit RNA chaperone activity during the cold adaptation process. ..

More Information


  1. El Sharoud W, Graumann P. Cold shock proteins aid coupling of transcription and translation in bacteria. Sci Prog. 2007;90:15-27 pubmed
    ..Members of both protein families have recently been shown to interact, suggesting that a two-step process achieves the coupling process, removal of secondary mRNA structures through CSHs and prevention of reformation through CSPs. ..
  2. Pai E. Familiar jaws with new twists. Nat Struct Biol. 1996;3:307-9 pubmed
  3. Fang L, Hou Y, Inouye M. Role of the cold-box region in the 5' untranslated region of the cspA mRNA in its transient expression at low temperature in Escherichia coli. J Bacteriol. 1998;180:90-5 pubmed
    Upon temperature downshift, a group of proteins called cold shock proteins, such as CspA, CspB, and CsdA, are transiently induced in Escherichia coli...
  4. Trun N, Johnston D. Folding chromosomes in bacteria: examining the role of Csp proteins and other small nucleic acid-binding proteins. Curr Top Dev Biol. 2003;55:173-201 pubmed
  5. Polissi A, De Laurentis W, Zangrossi S, Briani F, Longhi V, Pesole G, et al. Changes in Escherichia coli transcriptome during acclimatization at low temperature. Res Microbiol. 2003;154:573-80 pubmed
    ..Interestingly, we found that PNPase both negatively and positively modulated the transcript abundance of some of these genes, thus suggesting a complex role of PNPase in controlling cold adaptation. ..
  6. Phadtare S, Zhu L, Uemori T, Mukai H, Kato I, Inouye M. Applications of nucleic acid chaperone activity of CspA and its homologues. J Mol Microbiol Biotechnol. 2009;17:110-7 pubmed publisher
    ..The low substrate specificity of Csps together with their compatibility with various enzymes and their stability and activity over a broad temperature range makes them ideal candidates to be used for a variety of processes. ..