Gene Symbol: clpS
Description: regulatory protein for ClpA substrate specificity
Alias: ECK0872, JW0865, yljA
Species: Escherichia coli str. K-12 substr. MG1655
Products:     clpS

Top Publications

  1. Ninnis R, Spall S, Talbo G, Truscott K, Dougan D. Modification of PATase by L/F-transferase generates a ClpS-dependent N-end rule substrate in Escherichia coli. EMBO J. 2009;28:1732-44 pubmed publisher
    ..In Escherichia coli, model substrates bearing an N-degron are recognised by ClpS and degraded by ClpAP in an ATP-dependent manner...
  2. Schmidt R, Bukau B, Mogk A. Principles of general and regulatory proteolysis by AAA+ proteases in Escherichia coli. Res Microbiol. 2009;160:629-36 pubmed publisher
    ..Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recognition by AAA+ proteases and cognate adaptor proteins. ..
  3. Lupas A, Koretke K. Bioinformatic analysis of ClpS, a protein module involved in prokaryotic and eukaryotic protein degradation. J Struct Biol. 2003;141:77-83 pubmed
    b>ClpS is a small protein, usually encoded immediately upstream of ClpA in the genomes of proteobacteria. Recent results show that it is a molecular adaptor for substrate recognition by ClpA in Escherichia coli...
  4. Tasaki T, Zakrzewska A, Dudgeon D, Jiang Y, Lazo J, Kwon Y. The substrate recognition domains of the N-end rule pathway. J Biol Chem. 2009;284:1884-95 pubmed publisher
    ..The approximately 80-residue N-domain shows structural and functional similarity to 106-residue Escherichia coli ClpS, a bacterial N-recognin...
  5. Cranz Mileva S, Imkamp F, Kolygo K, Maglica Z, Kress W, Weber Ban E. The flexible attachment of the N-domains to the ClpA ring body allows their use on demand. J Mol Biol. 2008;378:412-24 pubmed publisher
    ..associated linker but is impaired only slightly in the processing of substrates that are degraded independently of ClpS. In fact, it shows increased catalytic efficiency in the degradation of ssrA-tagged GFP compared to ClpAwt...
  6. Zeth K, Dougan D, Cusack S, Bukau B, Ravelli R. Crystallization and preliminary X-ray analysis of the Escherichia coli adaptor protein ClpS, free and in complex with the N-terminal domain of ClpA. Acta Crystallogr D Biol Crystallogr. 2002;58:1207-10 pubmed was shown that the substrate recognition of the chaperone ClpA could be modified by the adaptor protein ClpS. To investigate the structural implications of this change in substrate specificity, ClpS was crystallized alone ..
  7. Schmidt R, Zahn R, Bukau B, Mogk A. ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway. Mol Microbiol. 2009;72:506-17 pubmed publisher
    ..In Escherichia coli the adaptor protein ClpS directly interacts with destabilizing N-terminal residues and transfers them to the ClpA/ClpP proteolytic complex ..
  8. Wang K, Oakes E, Sauer R, Baker T. Tuning the strength of a bacterial N-end rule degradation signal. J Biol Chem. 2008;283:24600-7 pubmed publisher
    ..the components of this pathway directly responsible for protein degradation are the ClpAP protease and its adaptor ClpS. We recently demonstrated that ClpAP is able to recognize N-end motifs in the absence of ClpS although with ..
  9. Wang K, Sauer R, Baker T. ClpS modulates but is not essential for bacterial N-end rule degradation. Genes Dev. 2007;21:403-8 pubmed
    In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP...

More Information


  1. Hou J, Sauer R, Baker T. Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Nat Struct Mol Biol. 2008;15:288-94 pubmed publisher
    Adaptor proteins modify substrate recognition by AAA+ ATPases. We examined how the adaptor ClpS regulates substrate choice by the Escherichia coli protease ClpAP...
  2. Hauser R, Ceol A, Rajagopala S, Mosca R, Siszler G, Wermke N, et al. A second-generation protein-protein interaction network of Helicobacter pylori. Mol Cell Proteomics. 2014;13:1318-29 pubmed publisher
    ..coli of which one third turned out to be conserved in both species. ..
  3. Erbse A, Schmidt R, Bornemann T, Schneider Mergener J, Mogk A, Zahn R, et al. ClpS is an essential component of the N-end rule pathway in Escherichia coli. Nature. 2006;439:753-6 pubmed
    ..Here we report that the ClpAP-specific adaptor, ClpS, is essential for degradation of N-end rule substrates by ClpAP in bacteria...
  4. Xia D, Esser L, Singh S, Guo F, Maurizi M. Crystallographic investigation of peptide binding sites in the N-domain of the ClpA chaperone. J Struct Biol. 2004;146:166-79 pubmed
    ..pseudo-dimer is a large flexible acidic loop that becomes better ordered upon binding of the small adaptor protein, ClpS. We have identified a number of structural features in the N-domain, including a Zn(++) binding motif, several ..
  5. Zeth K, Ravelli R, Paal K, Cusack S, Bukau B, Dougan D. Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA. Nat Struct Biol. 2002;9:906-11 pubmed publisher
    ..In some cases, however, the specificity is modified by adaptor proteins, such as ClpS. Here we report the 2.5 A resolution crystal structure of ClpS in complex with the N-terminal domain of ClpA...
  6. Farrell C, Grossman A, Sauer R. Cytoplasmic degradation of ssrA-tagged proteins. Mol Microbiol. 2005;57:1750-61 pubmed
    ..contributions to degradation of ssrA-tagged proteins in vivo have been uncertain because two adaptor proteins, ClpS and SspB, can modulate proteolysis of these substrates...
  7. Schuenemann V, Kralik S, Albrecht R, Spall S, Truscott K, Dougan D, et al. Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS. EMBO Rep. 2009;10:508-14 pubmed publisher
    In Escherichia coli, the ClpAP protease, together with the adaptor protein ClpS, is responsible for the degradation of proteins bearing an amino-terminal destabilizing amino acid (N-degron)...
  8. De Donatis G, Singh S, Viswanathan S, Maurizi M. A single ClpS monomer is sufficient to direct the activity of the ClpA hexamer. J Biol Chem. 2010;285:8771-81 pubmed publisher
    b>ClpS is an adaptor protein that interacts with ClpA and promotes degradation of proteins with N-end rule degradation motifs (N-degrons) by ClpAP while blocking degradation of substrates with other motifs...
  9. Dougan D, Reid B, Horwich A, Bukau B. ClpS, a substrate modulator of the ClpAP machine. Mol Cell. 2002;9:673-83 pubmed
    ..Here, we report the identification of a ClpA cofactor from Escherichia coli, ClpS, which directly influences the ClpAP machine by binding to the N-terminal domain of the chaperone ClpA...
  10. Zheng M, Wang X, Templeton L, Smulski D, LaRossa R, Storz G. DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide. J Bacteriol. 2001;183:4562-70 pubmed
    ..These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide. ..
  11. Maglica Z, Striebel F, Weber Ban E. An intrinsic degradation tag on the ClpA C-terminus regulates the balance of ClpAP complexes with different substrate specificity. J Mol Biol. 2008;384:503-11 pubmed publisher
    ..ClpXP is considered the primary recipient of ssrA-tagged substrates while ClpAP in complex with ClpS processes N-end rule substrates...
  12. Guo F, Esser L, Singh S, Maurizi M, Xia D. Crystal structure of the heterodimeric complex of the adaptor, ClpS, with the N-domain of the AAA+ chaperone, ClpA. J Biol Chem. 2002;277:46753-62 pubmed
    ..coli ATP-dependent protease, ClpAP, is modulated by an adaptor protein, ClpS. ClpS binds to ClpA, the regulatory component of the ClpAP complex...