Gene Symbol: chpB
Description: toxin of the ChpB-ChpS toxin-antitoxin system
Alias: ECK4221, JW4184, chpBK, yjfE
Species: Escherichia coli str. K-12 substr. MG1655
Products:     chpB

Top Publications

  1. Weiss D, Johnson D, Weith H, Somerville R. Structural analysis of the ileR locus of Escherichia coli K12. J Biol Chem. 1986;261:9966-71 pubmed
    ..S1 nuclease mapping of the 5' terminus of orf83 mRNA revealed two discrete species whose startpoints differed by approximately 27 nucleotides. The upstream promoters for ileR and orf83 overlap in their -35 regions. ..
  2. Belitsky M, Avshalom H, Erental A, Yelin I, Kumar S, London N, et al. The Escherichia coli extracellular death factor EDF induces the endoribonucleolytic activities of the toxins MazF and ChpBK. Mol Cell. 2011;41:625-35 pubmed publisher
    ..coli toxin MazF is a sequence-specific endoribonuclease cleaving single-stranded mRNAs at ACA sequences. E. coli ChpBK, a toxin homologous to MazF, is a sequence-specific endoribonuclease cleaving single-stranded mRNAs at ACA, ACG, ..
  3. Masuda Y, Ohtsubo E. Mapping and disruption of the chpB locus in Escherichia coli. J Bacteriol. 1994;176:5861-3 pubmed
    ..Like pem, chpB codes for two genes, chpBK and chpBI, encoding a growth inhibitor and a suppressor for the killing action of the ChpBK protein, respectively...
  4. Masuda Y, Miyakawa K, Nishimura Y, Ohtsubo E. chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100. J Bacteriol. 1993;175:6850-6 pubmed
    ..consists of two genes, chpAI and chpAK; the other, at 100 min, was named chpB and consists of two genes, chpBI and chpBK. The distal portion of chpBK was found to be adjacent to the ppa gene that encodes pyrophosphatase, whose map ..
  5. Santos Sierra S, Giraldo R, Diaz Orejas R. Functional interactions between chpB and parD, two homologous conditional killer systems found in the Escherichia coli chromosome and in plasmid R1. FEMS Microbiol Lett. 1998;168:51-8 pubmed
    ..They are bicistronic operons encoding a killer protein (Kid and ChpBK) and an antidote (Kis and ChpBI)...
  6. Gerdes K. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress. J Bacteriol. 2000;182:561-72 pubmed
  7. Westwater C, Kasman L, Schofield D, Werner P, Dolan J, Schmidt M, et al. Use of genetically engineered phage to deliver antimicrobial agents to bacteria: an alternative therapy for treatment of bacterial infections. Antimicrob Agents Chemother. 2003;47:1301-7 pubmed
    ..using phage as a lethal-agent delivery vehicle, we used the M13 phagemid system and the addiction toxins Gef and ChpBK. Phage delivery of lethal-agent phagemids reduced target bacterial numbers by several orders of magnitude in vitro ..
  8. Zhang Y, Zhu L, Zhang J, Inouye M. Characterization of ChpBK, an mRNA interferase from Escherichia coli. J Biol Chem. 2005;280:26080-8 pubmed
    ..b>ChpBK is a toxin encoded by the ChpBIK antitoxin-toxin module...
  9. Inouye M. The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology. J Cell Physiol. 2006;209:670-6 pubmed
    ..The discovery of a novel way to interfere with mRNA function by mRNA interferases opens a wide variety of avenues in basic as well as applied and clinical sciences. ..

More Information


  1. Tsilibaris V, Maenhaut Michel G, Mine N, Van Melderen L. What is the benefit to Escherichia coli of having multiple toxin-antitoxin systems in its genome?. J Bacteriol. 2007;189:6101-8 pubmed
    ..This casts a doubt on whether TA systems significantly influence bacterial fitness and competitiveness during non-steady-state growth conditions. ..
  2. Yamaguchi Y, Inouye M. mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems. Prog Mol Biol Transl Sci. 2009;85:467-500 pubmed publisher
    ..We propose that mRNA interferases play roles not only in cell growth regulation and programmed cell death, but also in regulation of specific gene expression (either positively or negatively) in bacteria. ..
  3. Kolodkin Gal I, Verdiger R, Shlosberg Fedida A, Engelberg Kulka H. A differential effect of E. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation. PLoS ONE. 2009;4:e6785 pubmed publisher
    ..Cell death governed by mazEF and dinJ-YafQ seems to participate in biofilm formation through a novel mechanism. ..
  4. Lahti R, Pitkäranta T, Valve E, Ilta I, Kukko Kalske E, Heinonen J. Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12. J Bacteriol. 1988;170:5901-7 pubmed
    ..coli PPase determined by S. A. Cohen (Ph.D. thesis, University of Chicago, 1978) was the same as that deduced from the nucleotide sequence. This is the first report of the cloning of a PPase gene. ..
  5. Christensen S, Pedersen K, Hansen F, Gerdes K. Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA. J Mol Biol. 2003;332:809-19 pubmed yield a mechanistic explanation for the detrimental effect on cell growth exerted by ChpAK and the homologous ChpBK protein of E.coli. We show that both proteins inhibit translation by inducing cleavage of translated mRNAs...