Gene Symbol: cbpA
Description: DnaK co-chaperone; curved DNA-binding protein
Alias: ECK0991, JW0985
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Chenoweth M, Trun N, Wickner S. In vivo modulation of a DnaJ homolog, CbpA, by CbpM. J Bacteriol. 2007;189:3635-8 pubmed
    b>CbpA, an Escherichia coli DnaJ homolog, can function as a cochaperone for the DnaK/Hsp70 chaperone system, and its in vitro activity can be modulated by CbpM...
  2. Yamashino T, Kakeda M, Ueguchi C, Mizuno T. An analogue of the DnaJ molecular chaperone whose expression is controlled by sigma s during the stationary phase and phosphate starvation in Escherichia coli. Mol Microbiol. 1994;13:475-83 pubmed
    The Escherichia coli CbpA protein appears to be an analogue of the molecular chaperone, DnaJ, as judged from not only its structure but also its possible function...
  3. Cosgriff S, Chintakayala K, Chim Y, Chen X, Allen S, Lovering A, et al. Dimerization and DNA-dependent aggregation of the Escherichia coli nucleoid protein and chaperone CbpA. Mol Microbiol. 2010;77:1289-300 pubmed publisher
    The Escherichia coli curved DNA-binding protein A (CbpA) is a nucleoid-associated DNA-binding factor and chaperone that is expressed at high levels as cells enter stationary phase...
  4. Ueguchi C, Shiozawa T, Kakeda M, Yamada H, Mizuno T. A study of the double mutation of dnaJ and cbpA, whose gene products function as molecular chaperones in Escherichia coli. J Bacteriol. 1995;177:3894-6 pubmed
    The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations...
  5. Azam T, Hiraga S, Ishihama A. Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells. 2000;5:613-26 pubmed
    ..regulatory activities of DNA functions accumulate at specific loci within the nucleoid, forming 2 (SeqA), 3-4 (CbpA and CbpB) and 6-10 (Fis and IciA) immuno-stained dots...
  6. Ueguchi C, Kakeda M, Yamada H, Mizuno T. An analogue of the DnaJ molecular chaperone in Escherichia coli. Proc Natl Acad Sci U S A. 1994;91:1054-8 pubmed
    ..This protein, named CbpA (for curved DNA-binding protein), was first identified as a DNA-binding protein that preferentially recognizes a ..
  7. Chintakayala K, Singh S, Rossiter A, Shahapure R, Dame R, Grainger D. E. coli Fis protein insulates the cbpA gene from uncontrolled transcription. PLoS Genet. 2013;9:e1003152 pubmed publisher
    The Escherichia coli curved DNA binding protein A (CbpA) is a poorly characterised nucleoid associated factor and co-chaperone. It is expressed at high levels as cells enter stationary phase...
  8. Ali Azam T, Iwata A, Nishimura A, Ueda S, Ishihama A. Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid. J Bacteriol. 1999;181:6361-70 pubmed
    ..coli W3110, including CbpA (curved DNA-binding protein A), CbpB (curved DNA-binding protein B, also known as Rob [right origin binding protein]..
  9. Azam T, Ishihama A. Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999;274:33105-13 pubmed
    ..We expressed and purified 12 species of the DNA-binding protein, i.e. CbpA (curved DNA-binding protein A), CbpB or Rob (curved DNA-binding protein B or right arm of the replication origin ..

More Information


  1. Bird J, Sharma S, Roshwalb S, Hoskins J, Wickner S. Functional analysis of CbpA, a DnaJ homolog and nucleoid-associated DNA-binding protein. J Biol Chem. 2006;281:34349-56 pubmed
    ..DnaJ/Hsp40 and GrpE are co-chaperones that assist DnaK. CbpA is an Escherichia coli DnaJ homolog...
  2. Chenoweth M, Wickner S. Complex regulation of the DnaJ homolog CbpA by the global regulators sigmaS and Lrp, by the specific inhibitor CbpM, and by the proteolytic degradation of CbpM. J Bacteriol. 2008;190:5153-61 pubmed publisher
    b>CbpA is a DnaJ homolog that functions as a DnaK cochaperone. Several cellular processes, including growth at low and high temperatures and septum formation during cell division, require either CbpA or DnaJ...
  3. Chae C, Sharma S, Hoskins J, Wickner S. CbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM. J Biol Chem. 2004;279:33147-53 pubmed
    ..b>CbpA, one of several DnaJ analogs in Escherichia coli, is known to function as a multicopy suppressor for dnaJ mutations ..
  4. Shiozawa T, Ueguchi C, Mizuno T. The rpoD gene functions as a multicopy suppressor for mutations in the chaperones, CbpA, DnaJ and DnaK, in Escherichia coli. FEMS Microbiol Lett. 1996;138:245-50 pubmed
    The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. The dnaJ- cbpA- double-null mutant exhibits severe defects in cell growth, namely, a very narrow temperature range for growth...
  5. Genevaux P, Schwager F, Georgopoulos C, Kelley W. The djlA gene acts synergistically with dnaJ in promoting Escherichia coli growth. J Bacteriol. 2001;183:5747-50 pubmed
    The DnaK chaperone of Escherichia coli is known to interact with the J domains of DnaJ, CbpA, and DjlA...
  6. Wegrzyn A, Taylor K, Wegrzyn G. The cbpA chaperone gene function compensates for dnaJ in lambda plasmid replication during amino acid starvation of Escherichia coli. J Bacteriol. 1996;178:5847-9 pubmed
    ..Here we demonstrate that this replication is inhibited when functions of both dnaJ and cbpA are impaired. In complete media, the growth of the lambda pi A66 phage (capable of replicating in E...
  7. Gur E, Katz C, Ron E. All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins. FEBS Lett. 2005;579:1935-9 pubmed
    DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well...
  8. Hennessy F, Nicoll W, Zimmermann R, Cheetham M, Blatch G. Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions. Protein Sci. 2005;14:1697-709 pubmed
    ..We also propose a model in which the J domain is able to integrate specificity and chaperone activity. ..
  9. Qiu X, Shao Y, Miao S, Wang L. The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. Cell Mol Life Sci. 2006;63:2560-70 pubmed
    ..This review highlights the progress in studying the domains in DnaJ/Hsp40 proteins, introduces the mechanisms by which they interact with Hsp70s, and stresses their functional diversity. ..
  10. Genevaux P, Georgopoulos C, Kelley W. The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol Microbiol. 2007;66:840-57 pubmed
    ..In particular, recent advances in structure determination have led to significant insights of the underlying complexities and functional elegance of the Hsp70 chaperone machine. ..
  11. Chintakayala K, Sellars L, Singh S, Shahapure R, Westerlaken I, Meyer A, et al. DNA recognition by Escherichia coli CbpA protein requires a conserved arginine-minor-groove interaction. Nucleic Acids Res. 2015;43:2282-92 pubmed publisher
    Curved DNA binding protein A (CbpA) is a co-chaperone and nucleoid associated DNA binding protein conserved in most γ-proteobacteria...
  12. Gur E, Biran D, Shechter N, Genevaux P, Georgopoulos C, Ron E. The Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation. J Bacteriol. 2004;186:7236-42 pubmed
    ..Two other E. coli proteins, CbpA and DjlA, which exhibit homology with DnaJ, are known to interact with DnaK and to stimulate its chaperone activity...
  13. Tatsuta T, Tomoyasu T, Bukau B, Kitagawa M, Mori H, Karata K, et al. Heat shock regulation in the ftsH null mutant of Escherichia coli: dissection of stability and activity control mechanisms of sigma32 in vivo. Mol Microbiol. 1998;30:583-93 pubmed
    ..In addition, CbpA, an analogue of DnaJ, was demonstrated to have overlapping functions with DnaJ in both the activity and the ..
  14. Tomoyasu T, Ogura T, Tatsuta T, Bukau B. Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli. Mol Microbiol. 1998;30:567-81 pubmed
    ..DnaK and DnaJ thus constitute the primary stress-sensing and transducing system of the E. coli heat shock response, which detects protein misfolding with high sensitivity. ..
  15. Nillegoda N, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, et al. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. elife. 2017;6: pubmed publisher
    ..This fundamental change in J-protein biology during the prokaryote-to-eukaryote transition allows for increased fine-tuning and broadening of Hsp70 function in eukaryotes. ..