Gene Symbol: can
Description: carbonic anhydrase
Alias: ECK0125, JW0122, yadF
Species: Escherichia coli str. K-12 substr. MG1655
Products:     can

Top Publications

  1. Kozliak E, Guilloton M, Fuchs J, Anderson P. Bacterial carbonic anhydrases. EXS. 2000;:547-65 pubmed
  2. Sevcenco A, Pinkse M, Wolterbeek H, Verhaert P, Hagen W, Hagedoorn P. Exploring the microbial metalloproteome using MIRAGE. Metallomics. 2011;3:1324-30 pubmed publisher
    ..The zinc proteome of E. coli experiencing zinc stress was dominated by ZraP, a putative zinc storage protein. ..
  3. Salmon K, Hung S, Mekjian K, Baldi P, Hatfield G, Gunsalus R. Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR. J Biol Chem. 2003;278:29837-55 pubmed
    ..In particular, we describe an approach for identifying gene expression patterns (clusters) obtained from multiple perturbation experiments based on a subset of genes that exhibit high probability for differential expression values. ..
  4. Cronk J, Rowlett R, Zhang K, Tu C, Endrizzi J, Lee J, et al. Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase. Biochemistry. 2006;45:4351-61 pubmed publisher
    ..We hypothesize that the two structural subclasses of beta-CAs may provide models for the proposed active and inactive forms of the H. influenzae and E. coli enzymes...
  5. Tripp B, Smith K, Ferry J. Carbonic anhydrase: new insights for an ancient enzyme. J Biol Chem. 2001;276:48615-8 pubmed
  6. Cronk J, O Neill J, Cronk M, Endrizzi J, Zhang K. Cloning, crystallization and preliminary characterization of a beta-carbonic anhydrase from Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2000;56:1176-9 pubmed
    ..A crystal structure for CynT2 would contribute significantly to the emerging structural knowledge of a biologically important class of enzymes that perform critical functions in carbon fixation and prokaryotic metabolism. ..
  7. Smith K, Ferry J. Prokaryotic carbonic anhydrases. FEMS Microbiol Rev. 2000;24:335-66 pubmed
    ..Investigations into prokaryotic carbonic anhydrase have already led to the identification of a new class (gamma) and future research will undoubtedly reveal novel functions for carbonic anhydrase in prokaryotes. ..
  8. Hashimoto M, Kato J. Indispensability of the Escherichia coli carbonic anhydrases YadF and CynT in cell proliferation at a low CO2 partial pressure. Biosci Biotechnol Biochem. 2003;67:919-22 pubmed
    We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli...
  9. Bolanos Garcia V, Davies O. Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography. Biochim Biophys Acta. 2006;1760:1304-13 pubmed
    ..coli most commonly co-purified by IMAC, including Fur, Crp, ArgE, SlyD, GlmS, GlgA, ODO1, ODO2, YadF and YfbG...

More Information


  1. Cronk J, Endrizzi J, Cronk M, O Neill J, Zhang K. Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity. Protein Sci. 2001;10:911-22 pubmed
    ..The structural and biochemical characterizations of ECCA presented here and the comparisons with other beta-CA structures suggest that ECCA can adopt two distinct conformations displaying widely divergent catalytic rates.
  2. Merlin C, Masters M, McAteer S, Coulson A. Why is carbonic anhydrase essential to Escherichia coli?. J Bacteriol. 2003;185:6415-24 pubmed
    The can (previously yadF) gene of Escherichia coli encodes a beta-class carbonic anhydrase (CA), an enzyme which interconverts CO(2) and bicarbonate...