Gene Symbol: caiE
Description: stimulator of CaiD and CaiB enzyme activities
Alias: ECK0036, JW5004
Species: Escherichia coli str. K-12 substr. MG1655
Eichler K, Bourgis F, Buchet A, Kleber H, Mandrand Berthelot M. Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli. Mol Microbiol. 1994;13:775-86 pubmed
..predicted molecular masses of 56,613 Da (CaiT), 42,564 Da (CaiA), 59,311 Da (CaiC), 32,329 Da (CaiD) and 21,930 Da (CaiE). Amino acid sequence similarity or enzymatic analysis supported the function assigned to each protein...
Eichler K, Buchet A, Lemke R, Kleber H, Mandrand Berthelot M. Identification and characterization of the caiF gene encoding a potential transcriptional activator of carnitine metabolism in Escherichia coli. J Bacteriol. 1996;178:1248-57 pubmed
..The histone-like protein H-NS and the NarL (plus nitrate) regulator acted as repressors. Because of the multiple controls to which the caiF gene is subjected, it appears to be a key element in the regulation of carnitine metabolism...
Buchet A, Eichler K, Mandrand Berthelot M. Regulation of the carnitine pathway in Escherichia coli: investigation of the cai-fix divergent promoter region. J Bacteriol. 1998;180:2599-608 pubmed
..This region is thought to be involved in transduction of the signal mediated by the CaiF regulator. ..
Smith K, Ferry J. Prokaryotic carbonic anhydrases. FEMS Microbiol Rev. 2000;24:335-66 pubmed
..Investigations into prokaryotic carbonic anhydrase have already led to the identification of a new class (gamma) and future research will undoubtedly reveal novel functions for carbonic anhydrase in prokaryotes. ..
Jiang J, Duan S, Zhang H. [Cloning and expression of caiDE genes in E. coli BL21(DE3)]. Wei Sheng Wu Xue Bao. 2000;40:586-90 pubmed
..After its transformation into BL21(DE3) strain and induction by IPTG, the recombinant strain containing pDSW2 expressed two apparent protein bands on SDS-PAGE, whose molecular weight were 30 kD and 24 kD individually. ..