bamE

Summary

Gene Symbol: bamE
Description: lipoprotein component of BamABCDE OM biogenesis complex
Alias: ECK2613, JW2598, smpA, smqA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Brokx S, Ellison M, Locke T, Bottorff D, Frost L, Weiner J. Genome-wide analysis of lipoprotein expression in Escherichia coli MG1655. J Bacteriol. 2004;186:3254-8 pubmed
    ..Additionally, these data serve as a test set for the refinement of high-throughput bioinformatic and global gene expression methods. ..
  2. Wu T, Malinverni J, Ruiz N, Kim S, Silhavy T, Kahne D. Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli. Cell. 2005;121:235-45 pubmed
    ..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process. ..
  3. Schleiff E, Soll J. Membrane protein insertion: mixing eukaryotic and prokaryotic concepts. EMBO Rep. 2005;6:1023-7 pubmed
    ..This review focuses on recent advances in the elucidation of the evolutionarily conserved concepts that underlie the translocation and insertion of beta-barrel membrane proteins. ..
  4. Sklar J, Wu T, Gronenberg L, Malinverni J, Kahne D, Silhavy T. Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli. Proc Natl Acad Sci U S A. 2007;104:6400-5 pubmed
    ..Here, we report another component of the YaeT complex, the OM lipoprotein small protein A (SmpA). Strains carrying loss-of-function mutations in smpA are viable but exhibit defects in OMP assembly...
  5. Bakelar J, Buchanan S, Noinaj N. The structure of the β-barrel assembly machinery complex. Science. 2016;351:180-6 pubmed publisher
    ..Further, conformational shifts in the barrel domain lead to opening of the exit pore and rearrangement at the lateral gate. ..
  6. Knowles T, Browning D, Jeeves M, Maderbocus R, Rajesh S, Sridhar P, et al. Structure and function of BamE within the outer membrane and the ?-barrel assembly machine. EMBO Rep. 2011;12:123-8 pubmed publisher
    ..Here, we report the native structure and mechanism of a core component of this complex, BamE, and show that it is exclusively monomeric in its native environment of the periplasm, but is able to adopt a ..
  7. Sauri A, Soprova Z, Wickström D, de Gier J, van der Schors R, Smit A, et al. The Bam (Omp85) complex is involved in secretion of the autotransporter haemoglobin protease. Microbiology. 2009;155:3982-91 pubmed publisher
    ..The data suggest a critical role for this general machinery in the translocation of autotransporters across the outer membrane. ..
  8. Glover W, Yang Y, Zhang Y. Insights into the molecular basis of L-form formation and survival in Escherichia coli. PLoS ONE. 2009;4:e7316 pubmed publisher
    ..acid capsule synthesis, ruvA, a recombinational junction binding protein, fur, a ferric uptake regulator and smpA a small membrane lipoprotein were selected for complementation...
  9. Knowles T, Scott Tucker A, Overduin M, Henderson I. Membrane protein architects: the role of the BAM complex in outer membrane protein assembly. Nat Rev Microbiol. 2009;7:206-14 pubmed publisher
    ..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins. ..

More Information

Publications19

  1. Gatsos X, Perry A, Anwari K, Dolezal P, Wolynec P, Likic V, et al. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiol Rev. 2008;32:995-1009 pubmed publisher
  2. Han L, Zheng J, Wang Y, Yang X, Liu Y, Sun C, et al. Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins. Nat Struct Mol Biol. 2016;23:192-6 pubmed publisher
    ..its five polypeptide transport-associated (POTRA) domains interact with the four lipoproteins BamB, BamC, BamD and BamE, thus forming the hat's brim in the periplasm...
  3. Albrecht R, Zeth K. Structural basis of outer membrane protein biogenesis in bacteria. J Biol Chem. 2011;286:27792-803 pubmed publisher
    ..By contrast, the dimeric BamE is formed through a domain swap and indicates fold similarity to the ?-lactamase inhibitor protein family, possibly ..
  4. Sabina J, Dover N, Templeton L, Smulski D, Soll D, LaRossa R. Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12. J Bacteriol. 2003;185:6158-70 pubmed
    ..Exposure of a relA strain to mupirocin resulted in accumulation of ribosomal protein operon transcripts. However, the relA strain's response to the other inhibitors was quite similar to that of the wild-type strain. ..
  5. Wei Y, Lee J, Richmond C, Blattner F, Rafalski J, LaRossa R. High-density microarray-mediated gene expression profiling of Escherichia coli. J Bacteriol. 2001;183:545-56 pubmed
    ..Genes the expression of which was detected by this method were enumerated, and results were compared with the current understanding of E. coli physiology. ..
  6. Miczak A, Chauhan A, Apirion D. Two new genes located between 2758 and 2761 kilobase pairs on the Escherichia coli genome. J Bacteriol. 1991;173:3271-2 pubmed
  7. Rigel N, Schwalm J, Ricci D, Silhavy T. BamE modulates the Escherichia coli beta-barrel assembly machine component BamA. J Bacteriol. 2012;194:1002-8 pubmed publisher
    ..complex is composed of the essential proteins BamA and BamD and three nonessential lipoproteins, BamB, BamC, and BamE. Both BamC and BamE are important for stabilizing the interaction between BamA and BamD...
  8. Ross J, Eady E, Cove J, Baumberg S. Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact. Gene. 1995;153:93-8 pubmed
    ..Two genes, stpA and smpA, were identified upstream from msrA on the original Staphylococcus epidermidis plasmid, encoding an ATP-binding ..
  9. Hagan C, Kim S, Kahne D. Reconstitution of outer membrane protein assembly from purified components. Science. 2010;328:890-2 pubmed publisher
    ..The assembly of this protein occurred without an energy source but required a soluble chaperone in addition to the multi-protein assembly complex. ..
  10. Rezuchova B, Miticka H, Homerova D, Roberts M, Kormanec J. New members of the Escherichia coli sigmaE regulon identified by a two-plasmid system. FEMS Microbiol Lett. 2003;225:1-7 pubmed
    ..tufA, recR), phospholipid and lipopolysaccharide biosynthesis (psd, lpxP), signal transduction (sixA), proposed inner or outer membrane proteins (bacA, sbmA, smpA, yeaY), and proteins with unknown function (ybaB, yaiW, yiiS, yiiT, yfeY).