bamA

Summary

Gene Symbol: bamA
Description: BamABCDE complex OM biogenesis outer membrane pore-forming assembly factor
Alias: ECK0176, JW0172, ecfK, omp85, yaeT, yzzN, yzzY
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Wu T, Malinverni J, Ruiz N, Kim S, Silhavy T, Kahne D. Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli. Cell. 2005;121:235-45 pubmed
    ..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process. ..
  2. Jain S, Goldberg M. Requirement for YaeT in the outer membrane assembly of autotransporter proteins. J Bacteriol. 2007;189:5393-8 pubmed
    ..Here, we demonstrate that secretion of autotransporters from several organisms requires the outer membrane assembly factor YaeT.
  3. Robert V, Volokhina E, Senf F, Bos M, van Gelder P, Tommassen J. Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-terminal motif. PLoS Biol. 2006;4:e377 pubmed
    ..The assembly of these proteins requires a proteinaceous apparatus of which Omp85 is an evolutionary conserved central component...
  4. Ruiz N, Wu T, Kahne D, Silhavy T. Probing the barrier function of the outer membrane with chemical conditionality. ACS Chem Biol. 2006;1:385-95 pubmed
    ..We report here that a suppressor mutation in yaeT , which specifies an essential protein involved in the assembly of beta-barrel proteins in the OM, confers ..
  5. Sanchez Pulido L, Devos D, Genevrois S, Vicente M, Valencia A. POTRA: a conserved domain in the FtsQ family and a class of beta-barrel outer membrane proteins. Trends Biochem Sci. 2003;28:523-6 pubmed
  6. Misra R. First glimpse of the crystal structure of YaeT's POTRA domains. ACS Chem Biol. 2007;2:649-51 pubmed
    The Omp85/YaeT family of proteins, which are conserved from bacteria to human, catalyzes insertion and assembly of proteins in the outer membrane...
  7. Vuong P, Bennion D, Mantei J, Frost D, Misra R. Analysis of YfgL and YaeT interactions through bioinformatics, mutagenesis, and biochemistry. J Bacteriol. 2008;190:1507-17 pubmed publisher
    In Escherichia coli, YaeT, together with four lipoproteins, YfgL, YfiO, NlpB, and SmpA, forms a complex that is essential for beta-barrel outer membrane protein biogenesis...
  8. Schleiff E, Soll J. Membrane protein insertion: mixing eukaryotic and prokaryotic concepts. EMBO Rep. 2005;6:1023-7 pubmed
    ..In particular, the identification of the Omp85/YaeT-involving pathways in Neisseria meningitidis, Escherichia coli and cyanobacteria, and homologues of Omp85/..
  9. Bayan N, Guilvout I, Pugsley A. Secretins take shape. Mol Microbiol. 2006;60:1-4 pubmed
    ..We compare them with other lipoproteins that play a role in Omp85/YaeT-mediated assembly of more classical outer membrane proteins...

More Information

Publications64

  1. Lehr U, Schutz M, Oberhettinger P, Ruiz Perez F, Donald J, Palmer T, et al. C-terminal amino acid residues of the trimeric autotransporter adhesin YadA of Yersinia enterocolitica are decisive for its recognition and assembly by BamA. Mol Microbiol. 2010;78:932-46 pubmed publisher
    ..It is composed of the essential outer membrane protein BamA and four outer membrane associated lipoproteins BamB-E...
  2. Aoki S, Malinverni J, Jacoby K, Thomas B, Pamma R, Trinh B, et al. Contact-dependent growth inhibition requires the essential outer membrane protein BamA (YaeT) as the receptor and the inner membrane transport protein AcrB. Mol Microbiol. 2008;70:323-40 pubmed publisher
    ..Characterization of mutants resistant to CDI allowed us to identify BamA (YaeT) as the outer membrane receptor for CDI and AcrB as a potential downstream target...
  3. Leonard Rivera M, Misra R. Conserved residues of the putative L6 loop of Escherichia coli BamA play a critical role in the assembly of ?-barrel outer membrane proteins, including that of BamA itself. J Bacteriol. 2012;194:4662-8 pubmed publisher
    Many members of the Omp85 family of proteins form essential ?-barrel outer membrane protein (OMP) biogenesis machinery in Gram-negative bacteria, chloroplasts, and mitochondria...
  4. Sklar J, Wu T, Kahne D, Silhavy T. Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Genes Dev. 2007;21:2473-84 pubmed
    ..Sec machinery, and assembled in the outer membrane through an unknown mechanism that requires the outer membrane YaeT complex and the periplasmic chaperones SurA, DegP, and Skp...
  5. Doerrler W, Raetz C. Loss of outer membrane proteins without inhibition of lipid export in an Escherichia coli YaeT mutant. J Biol Chem. 2005;280:27679-87 pubmed
    ..yaeT encodes an essential, conserved outer membrane (OM) protein that is an ortholog of Neisseria meningitidis Omp85. Conflicting data with N...
  6. Sklar J, Wu T, Gronenberg L, Malinverni J, Kahne D, Silhavy T. Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli. Proc Natl Acad Sci U S A. 2007;104:6400-5 pubmed
    ..We have previously shown that, in Escherichia coli, the multicomponent YaeT complex is responsible for the assembly of OM beta-barrel proteins (OMPs)...
  7. Bennion D, Charlson E, Coon E, Misra R. Dissection of ?-barrel outer membrane protein assembly pathways through characterizing BamA POTRA 1 mutants of Escherichia coli. Mol Microbiol. 2010;77:1153-71 pubmed publisher
    b>BamA of Escherichia coli is an essential component of the hetero-oligomeric machinery that mediates ?-barrel outer membrane protein (OMP) assembly...
  8. Knowles T, Jeeves M, Bobat S, Dancea F, McClelland D, Palmer T, et al. Fold and function of polypeptide transport-associated domains responsible for delivering unfolded proteins to membranes. Mol Microbiol. 2008;68:1216-27 pubmed publisher
    ..coli, folding substrates are inserted into the outer membrane by the essential protein YaeT, a prototypic Omp85 protein...
  9. Werner J, Misra R. YaeT (Omp85) affects the assembly of lipid-dependent and lipid-independent outer membrane proteins of Escherichia coli. Mol Microbiol. 2005;57:1450-9 pubmed
    The Omp85 family of proteins has been found in all Gram-negative bacteria and even several eukaryotic organisms. The previously uncharacterized Escherichia coli member of this family is YaeT...
  10. Gatzeva Topalova P, Walton T, Sousa M. Crystal structure of YaeT: conformational flexibility and substrate recognition. Structure. 2008;16:1873-81 pubmed publisher
    ..The Omp85 family of proteins, ubiquitous among Gram-negative bacteria and also present in chloroplasts and mitochondria, is ..
  11. Knowles T, Scott Tucker A, Overduin M, Henderson I. Membrane protein architects: the role of the BAM complex in outer membrane protein assembly. Nat Rev Microbiol. 2009;7:206-14 pubmed publisher
    ..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins. ..
  12. Kim S, Malinverni J, Sliz P, Silhavy T, Harrison S, Kahne D. Structure and function of an essential component of the outer membrane protein assembly machine. Science. 2007;317:961-4 pubmed
    ..The machine that assembles these proteins contains an integral membrane protein, called YaeT in Escherichia coli, which has one or more polypeptide transport-associated (POTRA) domains...
  13. Malinverni J, Werner J, Kim S, Sklar J, Kahne D, Misra R, et al. YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli. Mol Microbiol. 2006;61:151-64 pubmed
    Recent advances in the study of bacterial membranes have led to the identification of a multicomponent YaeT complex in the outer membrane (OM) of Gram-negative bacteria that is involved in the targeting and folding of beta-barrel outer ..
  14. Dwyer R, Ricci D, Colwell L, Silhavy T, Wingreen N. Predicting functionally informative mutations in Escherichia coli BamA using evolutionary covariance analysis. Genetics. 2013;195:443-55 pubmed publisher
    The essential outer membrane ?-barrel protein BamA forms a complex with four lipoprotein partners BamBCDE that assembles ?-barrel proteins into the outer membrane of Escherichia coli...
  15. Hagan C, Kahne D. The reconstituted Escherichia coli Bam complex catalyzes multiple rounds of ?-barrel assembly. Biochemistry. 2011;50:7444-6 pubmed publisher
    ..We use these conditions to analyze the effect of a mutation in the Bam complex and to demonstrate the ability of the reconstituted complex to catalyze more than one round of substrate assembly. ..
  16. Fleming P, Patel D, Wu E, Qi Y, Yeom M, Sousa M, et al. BamA POTRA Domain Interacts with a Native Lipid Membrane Surface. Biophys J. 2016;110:2698-2709 pubmed publisher
    ..coli, the BAM complex consists of five subunits, a core transmembrane ?-barrel with a long periplasmic domain (BamA) and four lipoproteins (BamB/C/D/E)...
  17. Bakelar J, Buchanan S, Noinaj N. The structure of the β-barrel assembly machinery complex. Science. 2016;351:180-6 pubmed publisher
    ..structure of the BAM complex from Escherichia coli, revealing that binding of BamCDE modulates the conformation of BamA, the central component, which may serve to regulate the BAM complex...
  18. Zhang H, Gao Z, Hou H, Xu J, Li L, Su X, et al. High-resolution structure of a new crystal form of BamA POTRA4-5 from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:734-8 pubmed publisher
    ..b>BamA, which is an essential component of the complex, consists of a C-terminal transmembrane region and five N-terminal ..
  19. Qu J, Mayer C, Behrens S, Holst O, Kleinschmidt J. The trimeric periplasmic chaperone Skp of Escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. J Mol Biol. 2007;374:91-105 pubmed
    ..These were OmpA, OmpG, and YaeT (Omp85) from Escherichia coli, the translocator domain of the autotransporter NalP from Neisseria meningitides, FomA from ..
  20. Ieva R, Bernstein H. Interaction of an autotransporter passenger domain with BamA during its translocation across the bacterial outer membrane. Proc Natl Acad Sci U S A. 2009;106:19120-5 pubmed publisher
    ..Using a site-specific photocrosslinking approach, we found that residues adjacent to the stall point interact with BamA, a component of a heterooligomeric complex (Bam complex) that catalyzes OM protein assembly, and that residues ..
  21. Sauri A, Soprova Z, Wickström D, de Gier J, van der Schors R, Smit A, et al. The Bam (Omp85) complex is involved in secretion of the autotransporter haemoglobin protease. Microbiology. 2009;155:3982-91 pubmed publisher
    ..The intermediate was copurified with the periplasmic chaperone SurA and subunits of the Bam (Omp85) complex that catalyse the insertion and assembly of outer-membrane proteins...
  22. Gatsos X, Perry A, Anwari K, Dolezal P, Wolynec P, Likic V, et al. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiol Rev. 2008;32:995-1009 pubmed publisher
  23. Aoki S, Webb J, Braaten B, Low D. Contact-dependent growth inhibition causes reversible metabolic downregulation in Escherichia coli. J Bacteriol. 2009;191:1777-86 pubmed publisher
    ..in Escherichia coli by which bacteria expressing two-partner secretion proteins encoded by cdiA and cdiB bind to BamA in the outer membranes of target cells and inhibit their growth...
  24. Dong C, Yang X, Hou H, Shen Y, Dong Y. Structure of Escherichia coli BamB and its interaction with POTRA domains of BamA. Acta Crystallogr D Biol Crystallogr. 2012;68:1134-9 pubmed publisher
    ..The BAM complex is comprised of an integral ?-barrel outer membrane protein BamA and four accessory lipoproteins BamB, BamC, BamD and BamE. Here, the crystal structure of BamB is reported...
  25. Albrecht R, Zeth K. Structural basis of outer membrane protein biogenesis in bacteria. J Biol Chem. 2011;286:27792-803 pubmed publisher
    ..functionality of BAM in protein biogenesis is mainly orchestrated through the presence of two essential components, BamA and BamD. Here, we present crystal structures of four lipoproteins (BamB-E)...
  26. Stegmeier J, Andersen C. Characterization of pores formed by YaeT (Omp85) from Escherichia coli. J Biochem. 2006;140:275-83 pubmed
    Proteins of the Omp85 family play a major role in the biogenesis of the bacterial outer membrane, since they were shown to mediate insertion of outer membrane proteins...
  27. Mahoney T, Ricci D, Silhavy T. Classifying β-Barrel Assembly Substrates by Manipulating Essential Bam Complex Members. J Bacteriol. 2016;198:1984-92 pubmed publisher
    ..We utilized these mutations, as well as a previously characterized mutation that lowers wild-type BamA levels, to reveal a third class of substrates...
  28. Han L, Zheng J, Wang Y, Yang X, Liu Y, Sun C, et al. Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins. Nat Struct Mol Biol. 2016;23:192-6 pubmed publisher
    ..55-Å resolution. The structure revealed that the BAM complex assembles into a hat-like shape, in which the BamA β-barrel domain forms the hat's crown embedded in the outer membrane, and its five polypeptide transport-..
  29. Browning D, Matthews S, Rossiter A, Sevastsyanovich Y, Jeeves M, Mason J, et al. Mutational and topological analysis of the Escherichia coli BamA protein. PLoS ONE. 2013;8:e84512 pubmed publisher
    ..An essential component of this complex is the BamA protein, which binds unfolded β-barrel precursors via the five polypeptide transport-associated (POTRA) domains ..
  30. Wzorek J, Lee J, Tomasek D, Hagan C, KAHNE D. Membrane integration of an essential β-barrel protein prerequires burial of an extracellular loop. Proc Natl Acad Sci U S A. 2017;114:2598-2603 pubmed publisher
    ..understand this process better, we studied the assembly of an essential β-barrel substrate for the Bam complex, BamA. By mutating conserved residues in the β-barrel domain of this protein, we generated three assembly-defective ..
  31. Cho S, Szewczyk J, Pesavento C, Zietek M, Banzhaf M, Roszczenko P, et al. Detecting envelope stress by monitoring β-barrel assembly. Cell. 2014;159:1652-64 pubmed publisher
    ..RcsF interacts with BamA, the major component of the β-barrel assembly machinery...
  32. Noinaj N, Kuszak A, Balusek C, GUMBART J, Buchanan S. Lateral opening and exit pore formation are required for BamA function. Structure. 2014;22:1055-62 pubmed publisher
    ..Crystal structures and molecular dynamics (MD) simulations of the central and essential component, BamA, suggest a mechanism involving lateral opening of its barrel domain...
  33. Ward R, Zoltner M, Beer L, El Mkami H, Henderson I, Palmer T, et al. The orientation of a tandem POTRA domain pair, of the beta-barrel assembly protein BamA, determined by PELDOR spectroscopy. Structure. 2009;17:1187-94 pubmed publisher
    ..have attempted to define the structure and articulation of the POTRA domains of the Escherichia coli, prototypic Omp85 protein BamA...
  34. Lee J, Xue M, Wzorek J, Wu T, Grabowicz M, Gronenberg L, et al. Characterization of a stalled complex on the ?-barrel assembly machine. Proc Natl Acad Sci U S A. 2016;113:8717-22 pubmed publisher
    ..folded around LptE, and both components interact with the two essential ?-barrel assembly machine (Bam) components, BamA and BamD...
  35. Zheng Y, Roberts R, Kasif S. Genomic functional annotation using co-evolution profiles of gene clusters. Genome Biol. 2002;3:RESEARCH0060 pubmed
    ..We show that the gene-pair phylogenetic profile is more accurate than the single-gene phylogenetic profiles. ..
  36. Bredemeier R, Schlegel T, Ertel F, Vojta A, Borissenko L, Bohnsack M, et al. Functional and phylogenetic properties of the pore-forming beta-barrel transporters of the Omp85 family. J Biol Chem. 2007;282:1882-90 pubmed
    beta-Barrel-shaped channels of the Omp85 family are involved in the translocation or assembly of proteins of bacterial, mitochondrial, and plastidic outer membranes...
  37. Gu Y, Zeng Y, Wang Z, Dong C. BamA ?16C strand and periplasmic turns are critical for outer membrane protein insertion and assembly. Biochem J. 2017;474:3951-3961 pubmed publisher
    ..The outer membrane proteins (OMPs) are inserted and assembled into the OM by OMP85 family proteins...
  38. Bernstein H. Are bacterial 'autotransporters' really transporters?. Trends Microbiol. 2007;15:441-7 pubmed
    ..by an exogenous factor (possibly a newly identified factor necessary for assembly of outer membrane proteins called 'Omp85/YaeT'), whereas the beta domain only targets the protein to the outer membrane and serves as a membrane anchor.
  39. Ni D, Wang Y, Yang X, Zhou H, Hou X, Cao B, et al. Structural and functional analysis of the ?-barrel domain of BamA from Escherichia coli. FASEB J. 2014;28:2677-85 pubmed publisher
    ..potential surface cavity, filled by a portion of the L6 loop that includes the conserved VRGF/Y motif found in the Omp85 family...
  40. Workman P, Heide K, Giuliano N, Lee N, Mar J, Vuong P, et al. Genetic, biochemical, and molecular characterization of the polypeptide transport-associated domain of Escherichia coli BamA. J Bacteriol. 2012;194:3512-21 pubmed publisher
    The BamA protein of Escherichia coli plays a central role in the assembly of ?-barrel outer membrane proteins (OMPs)...
  41. Ricci D, Hagan C, Kahne D, Silhavy T. Activation of the Escherichia coli ?-barrel assembly machine (Bam) is required for essential components to interact properly with substrate. Proc Natl Acad Sci U S A. 2012;109:3487-91 pubmed publisher
    ..Insertion of ?-barrel proteins into the OM is mediated by a protein complex that contains the OMP BamA and four associated lipoproteins (BamBCDE)...
  42. Burgess N, Dao T, Stanley A, Fleming K. Beta-barrel proteins that reside in the Escherichia coli outer membrane in vivo demonstrate varied folding behavior in vitro. J Biol Chem. 2008;283:26748-58 pubmed publisher
    ..product (PagP), OmpT, outer membrane phospholipase A (OmpLa), the fadl gene product (FadL), the yaet gene product (Omp85), and OmpF...
  43. Smith D, James C, Sergeant M, Yaxian Y, Saunders J, McCarthy A, et al. Short-tailed stx phages exploit the conserved YaeT protein to disseminate Shiga toxin genes among enterobacteria. J Bacteriol. 2007;189:7223-33 pubmed
    ..Here we show that an essential and highly conserved gene product, YaeT, which has an essential role in the insertion of proteins in the gram-negative bacterial outer membrane, is the ..
  44. Plummer A, Fleming K. BamA Alone Accelerates Outer Membrane Protein Folding In Vitro through a Catalytic Mechanism. Biochemistry. 2015;54:6009-11 pubmed publisher
    Barrel assembly machinery protein A (BamA) plays a critical role in the biogenesis of outer membrane proteins (OMPs); however, a mechanistic understanding of its function is lacking...
  45. Charlson E, Werner J, Misra R. Differential effects of yfgL mutation on Escherichia coli outer membrane proteins and lipopolysaccharide. J Bacteriol. 2006;188:7186-94 pubmed
    YfgL together with NlpB, YfiO, and YaeT form a protein complex to facilitate the insertion of proteins into the outer membrane of Escherichia coli...
  46. Hagan C, Kim S, Kahne D. Reconstitution of outer membrane protein assembly from purified components. Science. 2010;328:890-2 pubmed publisher
    ..The assembly of this protein occurred without an energy source but required a soluble chaperone in addition to the multi-protein assembly complex. ..
  47. Sinnige T, Weingarth M, Renault M, Baker L, Tommassen J, Baldus M. Solid-state NMR studies of full-length BamA in lipid bilayers suggest limited overall POTRA mobility. J Mol Biol. 2014;426:2009-21 pubmed publisher
    The outer membrane protein BamA is the key player in ?-barrel assembly in Gram-negative bacteria...
  48. Collin S, Guilvout I, Chami M, Pugsley A. YaeT-independent multimerization and outer membrane association of secretin PulD. Mol Microbiol. 2007;64:1350-7 pubmed
    ..in strains producing PulS were unaffected when the levels of the essential outer membrane assembly factor YaeT(Omp85) were reduced by controlled expression of a paraBAD-yaeT transcriptional fusion...
  49. Knowles T, Bobat S, Jeeves M, Henderson I, Overduin M. Secondary structure and 1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain. Biomol NMR Assign. 2007;1:113-5 pubmed publisher
    ..shift assignments and secondary structure of the Escherichia coli YaeT POTRA domain; a domain found in the Omp85 family of proteins which is critical for insertion and folding of outer membrane proteins in Gram-negative ..
  50. McCabe A, Ricci D, Adetunji M, Silhavy T. Conformational Changes That Coordinate the Activity of BamA and BamD Allowing ?-Barrel Assembly. J Bacteriol. 2017;199: pubmed publisher
    ..The ?-barrel assembly machine (Bam), a five-member complex composed of ?-barrel OMP BamA and four associated lipoproteins, BamB, BamC, BamD, and BamE, folds and inserts OMPs into the outer membrane...
  51. Jansen K, Baker S, Sousa M. Crystal structure of BamB bound to a periplasmic domain fragment of BamA, the central component of the β-barrel assembly machine. J Biol Chem. 2015;290:2126-36 pubmed publisher
    ..BAM is a five-protein complex consisting of the β-barrel OMP BamA and lipoproteins BamB, -C, -D, and -E...
  52. Rigel N, Schwalm J, Ricci D, Silhavy T. BamE modulates the Escherichia coli beta-barrel assembly machine component BamA. J Bacteriol. 2012;194:1002-8 pubmed publisher
    ..In Escherichia coli, the Bam complex is composed of the essential proteins BamA and BamD and three nonessential lipoproteins, BamB, BamC, and BamE...
  53. Tommassen J. Biochemistry. Getting into and through the outer membrane. Science. 2007;317:903-4 pubmed
  54. Liang R, Liu J. In-frame deletion of Escherichia coli essential genes in complex regulon. Biotechniques. 2008;44:209-10, 212-5 pubmed
    ..We selected three essential genes as targets, yaeT, fabZ, and dnaE, which are components of the complex eight-gene regulon yaeT-hlpA-lpxD-fabZ-lpxA-1pxB-rnhB-dnaE...
  55. Stegmeier J, Glück A, Sukumaran S, Mäntele W, Andersen C. Characterisation of YtfM, a second member of the Omp85 family in Escherichia coli. Biol Chem. 2007;388:37-46 pubmed
    b>Omp85 proteins form a ubiquitous protein family, members of which are found in all Gram-negative bacteria. Omp85 of Neisseria meningitidis and YaeT of Escherichia coli are shown to be essential for outer membrane biogenesis...