Gene Symbol: asnA
Description: asparagine synthetase A
Alias: ECK3738, JW3722
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Cedar H, Schwartz J. The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J Biol Chem. 1969;244:4112-21 pubmed
  2. Cedar H, Schwartz J. The asparagine synthetase of Escherichia coli. II. Studies on mechanism. J Biol Chem. 1969;244:4122-7 pubmed
  3. Nakamura M, Yamada M, Hirota Y, Sugimoto K, Oka A, Takanami M. Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12. Nucleic Acids Res. 1981;9:4669-76 pubmed
    We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA...
  4. Kosiba B, Schleif R. Arabinose-inducible promoter from Escherichia coli. Its cloning from chromosomal DNA, identification as the araFG promoter and sequence. J Mol Biol. 1982;156:53-66 pubmed
  5. Poggio S, Domeinzain C, Osorio A, Camarena L. The nitrogen assimilation control (Nac) protein represses asnC and asnA transcription in Escherichia coli. FEMS Microbiol Lett. 2002;206:151-6 pubmed
    In this work, we show that the expression of the asnA and asnC genes is regulated by the availability of ammonium in the growth medium...
  6. Ravel J, Norton S, Humphreys J, Shive W. Asparagine biosynthesis in Lactobacillus arabinosus and its control by asparagine through enzyme inhibition and repression. J Biol Chem. 1962;237:2845-9 pubmed
  7. Kolodkin Gal I, Hazan R, Gaathon A, Carmeli S, Engelberg Kulka H. A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli. Science. 2007;318:652-5 pubmed
    ..Structural analysis revealed that EDF is a linear pentapeptide, Asn-Asn-Trp-Asn-Asn. Each of the five amino acids of EDF is important for its activity. ..
  8. von Meyenburg K, Hansen F, Riise E, Bergmans H, Meijer M, Messer W. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:121-8 pubmed
  9. Hirota Y, Yasuda S, Yamada M, Nishimura A, Sugimoto K, Sugisaki H, et al. Structural and functional properties of the Escherichia coli origin of DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:129-38 pubmed

More Information


  1. Sugiyama A, Kato H, Nishioka T, Oda J. Overexpression and purification of asparagine synthetase from Escherichia coli. Biosci Biotechnol Biochem. 1992;56:376-9 pubmed
    Overexpression of the asnA gene from Escherichia coli K-12 coding for asparagine synthetase (EC was achieved with a plasmid, pUNAd37, a derivative of pUC18, in E. coli...
  2. K├Âlling R, Lother H. AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli. J Bacteriol. 1985;164:310-5 pubmed
    ..It was shown that the 17-kilodalton gene, which is transcribed divergently from the adjacent asnA gene, codes for an activator of asnA transcription...
  3. Wackett L, Wanner B, Venditti C, Walsh C. Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. J Bacteriol. 1987;169:1753-6 pubmed
    ..Since psiD::lacZ(Mu d1) mutants cannot metabolize methylphosphonic acid, psiD may be the structural gene(s) for C-P lyase. ..
  4. Humbert R, Simoni R. Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli. J Bacteriol. 1980;142:212-20 pubmed
    ..The former asn locus is now called asnA, and the new gene is designated asnB. The asnB gene is located near gal...
  5. Felton J, Michaelis S, Wright A. Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J Bacteriol. 1980;142:221-8 pubmed
    Escherichia coli K-12 has two genes, asnA+ and asnB+, either one of which is able to satisfy the need of cells for asparagine...
  6. Buhk H, Messer W. The replication origin region of Escherichia coli: nucleotide sequence and functional units. Gene. 1983;24:265-79 pubmed
    ..The coding regions for proteins were identified, and the possible function of those proteins is discussed. Within oriC two extended systems of dyad symmetry were found, and their possible significance is considered. ..
  7. Nakatsu T, Kato H, Oda J. Crystal structure of asparagine synthetase reveals a close evolutionary relationship to class II aminoacyl-tRNA synthetase. Nat Struct Biol. 1998;5:15-9 pubmed
    ..The functional and structural similarities of both enzymes suggest that new enzymatic activities would generally follow the recruitment of a protein catalyzing a similar chemical reaction. ..