Gene Symbol: arsR
Description: arsenical resistance operon transcriptional repressor; autorepressor
Alias: ECK3486, JW3468, arsE
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Cai J, DuBow M. Expression of the Escherichia coli chromosomal ars operon. Can J Microbiol. 1996;42:662-71 pubmed
    ..DNA sequencing revealed three open reading frames homologous to the arsR, arsB, and arsC open reading frames of plasmid-based arsenic resistance operons isolated from both E...
  2. Xu C, Shi W, Rosen B. The chromosomal arsR gene of Escherichia coli encodes a trans-acting metalloregulatory protein. J Biol Chem. 1996;271:2427-32 pubmed
    ..The arsR gene, the first gene in this operon, was cloned using polymerase chain reaction...
  3. Shi W, Dong J, Scott R, Ksenzenko M, Rosen B. The role of arsenic-thiol interactions in metalloregulation of the ars operon. J Biol Chem. 1996;271:9291-7 pubmed
    ..the Escherichia coli plasmid R773 that confers arsenical and antimonial resistance is negatively regulated by the ArsR repressor...
  4. Kostal J, Yang R, Wu C, Mulchandani A, Chen W. Enhanced arsenic accumulation in engineered bacterial cells expressing ArsR. Appl Environ Microbiol. 2004;70:4582-7 pubmed
    The metalloregulatory protein ArsR, which offers high affinity and selectivity toward arsenite, was overexpressed in Escherichia coli in an attempt to increase the bioaccumulation of arsenic...
  5. Carlin A, Shi W, Dey S, Rosen B. The ars operon of Escherichia coli confers arsenical and antimonial resistance. J Bacteriol. 1995;177:981-6 pubmed
    ..Expression of the ars genes was inducible by arsenite. By Southern hybridization, the operon was found in all strains of E. coli examined but not in Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis. ..
  6. Busenlehner L, Pennella M, Giedroc D. The SmtB/ArsR family of metalloregulatory transcriptional repressors: Structural insights into prokaryotic metal resistance. FEMS Microbiol Rev. 2003;27:131-43 pubmed
    The SmtB/ArsR family of prokaryotic metalloregulatory transcriptional repressors represses the expression of operons linked to stress-inducing concentrations of di- and multivalent heavy metal ions...
  7. Qin J, Fu H, Ye J, Bencze K, Stemmler T, Rawlings D, et al. Convergent evolution of a new arsenic binding site in the ArsR/SmtB family of metalloregulators. J Biol Chem. 2007;282:34346-55 pubmed
    ..resistance operon that is controlled by an As(III)-responsive transcriptional repressor, AfArsR, a member of the ArsR/SmtB family of metalloregulators...
  8. Rosen B, Liu Z. Transport pathways for arsenic and selenium: a minireview. Environ Int. 2009;35:512-5 pubmed publisher
    ..In this review we discuss the membrane proteins that transport arsenic and selenium into cells, from bacteria to humans, as well as some of the efflux proteins involved in detoxification. ..
  9. Diorio C, Cai J, Marmor J, Shinder R, DuBow M. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria. J Bacteriol. 1995;177:2050-6 pubmed
    ..This chromosomal operon was cloned, sequenced, and found to consist of three cistrons which we named arsR, arsB, and arsC because of their strong homology to plasmid-borne ars operons...

More Information


  1. Shi W, Wu J, Rosen B. Identification of a putative metal binding site in a new family of metalloregulatory proteins. J Biol Chem. 1994;269:19826-9 pubmed
    The transcription of the ars operon is negatively controlled by the ArsR repressor and induced by arsenite and antimonite...
  2. Xu C, Rosen B. Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli. J Biol Chem. 1997;272:15734-8 pubmed
    ..The first gene in the operon, arsR, was previously shown to encode a homodimeric trans-acting metalloregulatory repressor protein...