Gene Symbol: aroH
Description: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible
Alias: ECK1702, JW1694
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Wahl S, Haunschild M, Oldiges M, Wiechert W. Unravelling the regulatory structure of biochemical networks using stimulus response experiments and large-scale model selection. Syst Biol (Stevenage). 2006;153:275-85 pubmed
    ..It is illustrated by the example of the aromatic amino acid synthesis pathway in Escherichia coli. ..
  2. Johansson L, Liden G. Transcriptome analysis of a shikimic acid producing strain of Escherichia coli W3110 grown under carbon- and phosphate-limited conditions. J Biotechnol. 2006;126:528-45 pubmed
    ..The reason for this difference is interpreted in terms of starvation for aromatic amino acids under carbon-limitation which is relieved under phosphate-limitation due to an upregulation of aroK and aroA. ..
  3. Camakaris J, Pittard J. Purification and properties of 3-deoxy-D-arabionheptulosonic acid-7-phosphate synthetase (trp) from Escherichia coli. J Bacteriol. 1974;120:590-7 pubmed
    ..This activity was restored upon the addition of Co(2+) or Mn(2+). Neither treatment with ethylenediaminetetraacetic acid nor addition of Co(2+) or Mn(2+) affected the activity of the feedback-resistant enzyme. ..
  4. Wallace B, Pittard J. Chromatography of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) on diethylaminoethyl cellulose: a correction. J Bacteriol. 1967;94:1279-80 pubmed
  5. Walker S, Parker E. Synthesis and evaluation of a mechanism-based inhibitor of a 3-deoxy-D-arabino heptulosonate 7-phosphate synthase. Bioorg Med Chem Lett. 2006;16:2951-4 pubmed
  6. Knop D, Draths K, Chandran S, Barker J, von Daeniken R, Weber W, et al. Hydroaromatic equilibration during biosynthesis of shikimic acid. J Am Chem Soc. 2001;123:10173-82 pubmed
    ..By apparently repressing shikimate transport, the aforementioned E. coli SP1.1/pKD12.138 synthesized 52 g/L of shikimic acid in 18% yield from glucose as a 14:1.0:3.0 shikimate/quinate/dehydroshikimate mixture. ..
  7. Ray J, Yanofsky C, Bauerle R. Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. J Bacteriol. 1988;170:5500-6 pubmed
    The nucleotide sequence of aroH, the structural gene for the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase [DAHPS(Trp)], is presented, and the deduced amino acid sequence of AroH is compared with that of the ..
  8. Borg Olivier S, Tarlinton D, Brown K. Defective regulation of the phenylalanine biosynthetic operon in mutants of the phenylalanyl-tRNA synthetase operon. J Bacteriol. 1987;169:1949-53 pubmed
    ..This operon is repressed in the wild type by phenylalanine. The mutation in three of these mutants mapped in the aroH-aroD region of the E. coli chromosome at 37 min...
  9. Jordan P, Bohle D, Ramilo C, Evans J. New insights into the metal center of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. Biochemistry. 2001;40:8387-96 pubmed

More Information


  1. Ray J, Bauerle R. Purification and properties of tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. J Bacteriol. 1991;173:1894-901 pubmed
    The aroH gene of Escherichia coli, which encodes the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isoenzyme of the common aromatic biosynthetic pathway, was cloned behind the tac promoter in expression plasmid ..
  2. Zurawski G, Gunsalus R, Brown K, Yanofsky C. Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli. J Mol Biol. 1981;145:47-73 pubmed
  3. Wallace B, Pittard J. Regulation of 3-deoxy-D-arabino-heptulosonic 7-phosphate acid synthetase activity in relation to the synthesis of the aromatic vitamins in Escherichia coli K-12. J Bacteriol. 1969;99:707-12 pubmed
    ..Under repressed conditions both DAHP synthetase (phe) and (trp), but not DAHP synthetase (tyr), were shown to contribute to vitamin production. ..
  4. Akowski J, Bauerle R. Steady-state kinetics and inhibitor binding of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tryptophan sensitive) from Escherichia coli. Biochemistry. 1997;36:15817-22 pubmed
    ..The results suggest an inhibition mechanism analogous to system C5 hyperbolic mixed-type inhibition with respect to erythrose-4-phosphate and partial noncompetitive inhibition with respect to phosphoenolpyruvate. ..
  5. Hudson G, Davidson B. Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12. J Mol Biol. 1984;180:1023-51 pubmed
  6. Wallace B, Pittard J. Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli. J Bacteriol. 1967;93:237-44 pubmed
    ..coli chromosome. Chromatography on diethylaminoethyl cellulose columns allowed the recognition of four peaks of activity. ..
  7. Camakaris J, Pittard J. Repression of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) and enzymes of the tryptophan pathway in Escherichia coli K-12. J Bacteriol. 1971;107:406-14 pubmed
    ..The mutation causing derepression is closely linked to aroH [the structural gene for DAHP synthetase (trp)] and occurs in a locus designated aroJ...
  8. Duncan K, Chaudhuri S, Campbell M, Coggins J. The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase. Biochem J. 1986;238:475-83 pubmed
    ..The complete polypeptide chain consists of 240 amino acid residues and has a calculated subunit Mr of 26,377. Transcript mapping revealed that aroD is a typical monocistronic gene. ..
  9. Brunelle A, Schleif R. Determining residue-base interactions between AraC protein and araI DNA. J Mol Biol. 1989;209:607-22 pubmed
    ..The missing contacts displayed by three AraC mutants are found within two major groove regions of the DNA and are spaced 21 base-pairs apart in a pattern indicating a direct repeat orientation for the subunits of AraC. ..
  10. Hudson G, Rellos P, Davidson B. Two promoters control the aroH gene of Escherichia coli. Gene. 1991;102:87-91 pubmed
    The aroH gene from Escherichia coli encodes 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase (Trp), one of three isoenzymes which catalyse the first committed step in the biosynthesis of aromatic amino acids and vitamins...
  11. Muday G, Johnson D, Somerville R, Herrmann K. The tyrosine repressor negatively regulates aroH expression in Escherichia coli. J Bacteriol. 1991;173:3930-2 pubmed
    ..isoenzyme of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Escherichia coli, encoded by the aroH gene, were elevated in tyrR and/or trpR mutants...
  12. Davies W, Davidson B. The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12. Nucleic Acids Res. 1982;10:4045-58 pubmed
    ..There is significant identity in the nucleotide sequences of aroG and aroH (the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (trp), indicating that these two genes have ..
  13. Pittard J, Camakaris J, Wallace B. Inhibition of 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase (trp) in Escherichia coli. J Bacteriol. 1969;97:1242-7 pubmed
    ..The enzymic activity of DAHP synthetase (trp) in the presence of CO(2+) is sensitive to inhibition by tryptophan. Mutant strains of E. coli have been isolated in which DAHP synthetase (trp) is feedback-resistant. ..
  14. Stephens C, Bauerle R. Analysis of the metal requirement of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. J Biol Chem. 1991;266:20810-7 pubmed
    ..The tetrameric DAHPS(Phe) exhibited positive homotropic cooperativity with respect to erythrose 4-phosphate, phophoenolpyruvate, and phenylalanine in the presence of all metals tested. ..