Gene Symbol: argP
Description: transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Alias: ECK2912, JW2883, can, canR, iciA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Han J, Kwon H, Yim J, Hwang D. Effect of IciA protein on the expression of the nrd gene encoding ribonucleoside diphosphate reductase in E. coli. Mol Gen Genet. 1998;259:610-4 pubmed
    ..We found that the IciA protein binds specifically to the AT-rich upstream region of nrd promoter...
  2. Lee Y, Lee H, Yim J, Hwang D. The binding of two dimers of IciA protein to the dnaA promoter 1P element enhances the binding of RNA polymerase to the dnaA promoter 1P. Nucleic Acids Res. 1997;25:3486-9 pubmed
    ..of the dnaA gene from the promoter 1P has been shown to be activated in vitro and in vivo by the binding of IciA protein to two sites on the dnaA promoter region [Lee, Y. S., Kim, H., and Hwang, D. S. (1996) Mol. Microbiol ...
  3. Azam T, Ishihama A. Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999;274:33105-13 pubmed
    ..factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid protein), IciA (inhibitor of chromosome initiation A), IHF (integration host factor), Lrp (leucine-responsive regulatory protein), ..
  4. Hwang D, Thony B, Kornberg A. IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication. J Biol Chem. 1992;267:2209-13 pubmed
    Specific binding of IciA protein to the 13-mers in the origin of a minichromosome (oriC) inhibits initiation of replication in vitro by blocking the opening of this region effected by the initiator DnaA protein (Hwang, D.S...
  5. Halsall D. Overproduction of lysine by mutant strains of Escherichia coli with defective lysine transport systems. Biochem Genet. 1975;13:109-24 pubmed
    ..A hypothesis regarding the nature of these mutations and their effects on the regulation of lysine biosynthesis is discussed. ..
  6. Marbaniang C, Gowrishankar J. Role of ArgP (IciA) in lysine-mediated repression in Escherichia coli. J Bacteriol. 2011;193:5985-96 pubmed publisher
    Initially identified as an inhibitor of oriC-initiated DNA replication in vitro, the ArgP or IciA protein of Escherichia coli has subsequently been described as a nucleoid-associated protein and also as a transcriptional regulator of ..
  7. Maas W. Mapping of genes involved in the synthesis of spermidine in Escherichia coli. Mol Gen Genet. 1972;119:1-9 pubmed
  8. Celis R. Mutant of Escherichia coli K-12 with defective phosphorylation of two periplasmic transport proteins. J Biol Chem. 1990;265:1787-93 pubmed
    ..Both periplasmic proteins can be phosphorylated by a single kinase. The enzyme exhibits a kinase activity and an ATPase activity...
  9. Peeters E, Nguyen Le Minh P, Foulquié Moreno M, Charlier D. Competitive activation of the Escherichia coli argO gene coding for an arginine exporter by the transcriptional regulators Lrp and ArgP. Mol Microbiol. 2009;74:1513-26 pubmed publisher
    ..Activation by Lrp interferes with the previously demonstrated activation of the argO promoter by ArgP. This interference results from the mutual inhibitory binding of the two activators to overlapping targets...

More Information


  1. Han J, Park J, Lee Y, Thony B, Hwang D. PhoB-dependent transcriptional activation of the iciA gene during starvation for phosphate in Escherichia coli. Mol Gen Genet. 1999;262:448-52 pubmed
    The IciA protein from Escherichia coli has been shown specifically to inhibit the in vitro initiation of chromosomal DNA replication. However, the in vivo role of IciA has not yet been established...
  2. Schwartz J, Maas W. Analysis of the inhibition of growth produced by canavanine in Escherichia coli. J Bacteriol. 1960;79:794-9 pubmed
  3. Pierard A, Glansdorff N, Yashphe J. Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake. Mol Gen Genet. 1972;118:235-45 pubmed
  4. Celis T, Rosenfeld H, Maas W. Mutant of Escherichia coli K-12 defective in the transport of basic amino acids. J Bacteriol. 1973;116:619-26 pubmed
    ..A mutant was studied in which the uptake of arginine, ornithine, and lysine is reduced. The mutation was found to affect both the common and the specific transport systems. ..
  5. Bouvier J, Stragier P, Morales V, Remy E, Gutierrez C. Lysine represses transcription of the Escherichia coli dapB gene by preventing its activation by the ArgP activator. J Bacteriol. 2008;190:5224-9 pubmed publisher
    ..genomic library for a gene that could alleviate lysine repression when present in multicopy led to the recovery of argP, a gene encoding an activating protein of the LysR-type family, known to use lysine as an effector...
  6. Laishram R, Gowrishankar J. Environmental regulation operating at the promoter clearance step of bacterial transcription. Genes Dev. 2007;21:1258-72 pubmed
    ..Escherichia coli argO gene, which encodes an arginine (Arg) exporter, requires the LysR-family regulator protein ArgP (previously called IciA) and is induced in the presence of Arg or its naturally occurring antimetabolite analog ..
  7. Celis R. Repression and activation of arginine transport genes in Escherichia coli K 12 by the ArgP protein. J Mol Biol. 1999;294:1087-95 pubmed
    ..The argP(+) gene was cloned and sequenced. Analysis of the sequenced gene revealed that it is identical with iciA, an E. coli gene that encodes an inhibitor of chromosomal initiation of replication in vitro...
  8. Lee Y, Hwang D. Occlusion of RNA polymerase by oligomerization of DnaA protein over the dnaA promoter of Escherichia coli. J Biol Chem. 1997;272:83-8 pubmed
    ..When the two dnaA promoters were bound and repressed by DnaA protein, the interaction of RNA polymerase with IciA protein, which is a specific inhibitor of initiation of in vitro E...
  9. Rosen B. Basic amino acid transport in Escherichia coli: properties of canavanine-resistant mutants. J Bacteriol. 1973;116:627-35 pubmed
    ..The lesion maps at min 56 of the E. coli linkage map, at or near the argP locus...
  10. Zhou P, Bogan J, Welch K, Pickett S, Wang H, Zaritsky A, et al. Gene transcription and chromosome replication in Escherichia coli. J Bacteriol. 1997;179:163-9 pubmed
    ..levels for two genes, minE and tus, did not fluctuate significantly, whereas the transcripts for one gene, iciA, appeared to increase transiently. The results support the idea that cell cycle timing in E...
  11. Ali Azam T, Iwata A, Nishimura A, Ueda S, Ishihama A. Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid. J Bacteriol. 1999;181:6361-70 pubmed
    ..factor for phage Q(beta)), H-NS (histone-like nucleoid structuring protein), HU (heat-unstable nucleoid protein), IciA (inhibitor of chromosome initiation A), IHF (integration host factor), Lrp (leucine-responsive regulatory protein), ..
  12. Celis T. Properties of an Escherichia coli K-12 mutant defective in the transport of arginine and ornithine. J Bacteriol. 1977;130:1234-43 pubmed
    ..The mutation maps at min 62 on the recalibrated linkage map of Escherichia coli K-12, in a locus closely linked or identical to argP.
  13. Ruiz J, Haneburger I, Jung K. Identification of ArgP and Lrp as transcriptional regulators of lysP, the gene encoding the specific lysine permease of Escherichia coli. J Bacteriol. 2011;193:2536-48 pubmed publisher
    ..In this study, the LysR-type transcriptional regulator ArgP was identified as the activator of lysP expression...
  14. Lee Y, Kim H, Hwang D. Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli. Mol Microbiol. 1996;19:389-96 pubmed
    ..coli, starts from two promoters, 1P and 2P. Gel-shift and DNase I-protection assays revealed that IciA protein, an inhibitor of initiation of in vitro E...
  15. Yoo S, Seol J, Woo S, Suh S, Hwang D, Ha D, et al. Hydrolysis of the IciA protein, an inhibitor of DNA replication initiation, by protease Do in Escherichia coli. FEBS Lett. 1993;327:17-20 pubmed
    The 33 kDa IciA protein, an inhibitor of replication initiation of the Escherichia coli chromosome, was found to be specifically cleaved to 27 kDa fragment by protease Do, the htrA gene product...
  16. Hwang D, Kornberg A. Opposed actions of regulatory proteins, DnaA and IciA, in opening the replication origin of Escherichia coli. J Biol Chem. 1992;267:23087-91 pubmed
    ..b>IciA protein, the inhibitor of initiation, binds the three 13-mers and blocks the opening of the region...
  17. Nandineni M, Laishram R, Gowrishankar J. Osmosensitivity associated with insertions in argP (iciA) or glnE in glutamate synthase-deficient mutants of Escherichia coli. J Bacteriol. 2004;186:6391-9 pubmed
    ..Insertions conferring an osmosensitive phenotype were identified in the proU, argP (formerly iciA), and glnE genes encoding a glycine betaine/proline transporter, a LysR-type transcriptional regulator, and the ..
  18. Hwang D, Kornberg A. A novel protein binds a key origin sequence to block replication of an E. coli minichromosome. Cell. 1990;63:325-31 pubmed
    ..The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation. ..
  19. Thony B, Hwang D, Fradkin L, Kornberg A. iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro. Proc Natl Acad Sci U S A. 1991;88:4066-70 pubmed
    ..The protein, previously referred to as the 33-kDa protein, has been named IciA (for inhibitor of chromosome initiation). The iciA gene is at 62.8 min on the chromosomal map...
  20. Azam T, Hiraga S, Ishihama A. Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells. 2000;5:613-26 pubmed
    ..functions accumulate at specific loci within the nucleoid, forming 2 (SeqA), 3-4 (CbpA and CbpB) and 6-10 (Fis and IciA) immuno-stained dots...
  21. Wei T, Bernander R. Interaction of the IciA protein with AT-rich regions in plasmid replication origins. Nucleic Acids Res. 1996;24:1865-72 pubmed
    ..The IciA protein, which is known to bind to the AT-rich repeat region in the Escherichia coli origin of chromosome ..
  22. Nandineni M, Gowrishankar J. Evidence for an arginine exporter encoded by yggA (argO) that is regulated by the LysR-type transcriptional regulator ArgP in Escherichia coli. J Bacteriol. 2004;186:3539-46 pubmed
    ..coli was identified in this study as a gene that is under the transcriptional control of argP (previously called iciA), which encodes a LysR-type transcriptional regulator protein...
  23. Hove Jensen B, Maigaard M. Escherichia coli rpiA gene encoding ribose phosphate isomerase A. J Bacteriol. 1993;175:5628-35 pubmed
    ..Host strains harboring the rpiA gene in a multicopy plasmid contained up to 42-fold as much ribose phosphate isomerase A activity as the haploid strain. ..
  24. Maas W. Genetic defects affecting an arginine permease and repression of arginine synthesis in Escherichia coli. Fed Proc. 1965;24:1239-42 pubmed
  25. Rosen B. Basic amino acid transport in Escherichia coli. J Biol Chem. 1971;246:3653-62 pubmed