araG

Summary

Gene Symbol: araG
Description: L-arabinose ABC transporter ATPase
Alias: ECK1898, JW1888
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hogg R. L-Arabinose transport and the L-arabinose binding protein of Escherichia coli. J Supramol Struct. 1977;6:411-7 pubmed
    ..The L-arabinose binding protein has been purified, partially characterized, crystallized, and sequenced. ..
  2. Kehres D, Hogg R. Escherichia coli K12 arabinose-binding protein mutants with altered transport properties. Protein Sci. 1992;1:1652-60 pubmed
    ..coli binds L-arabinose in the periplasm and delivers it to a cytoplasmic membrane complex consisting of the AraG and AraH proteins, for uptake into the cell...
  3. Khlebnikov A, Risa O, Skaug T, Carrier T, Keasling J. Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture. J Bacteriol. 2000;182:7029-34 pubmed
    ..This work demonstrates the importance of including a transport gene that is controlled independently of the inducer to achieve regulatable and consistent induction in all cells of the culture. ..
  4. Timmes A, Rodgers M, Schleif R. Biochemical and physiological properties of the DNA binding domain of AraC protein. J Mol Biol. 2004;340:731-8 pubmed
    ..A simple analysis relates the DNA binding affinities of monomers of DNA binding domain and normal dimeric AraC protein. ..
  5. Hendrickson W, Rudd K. Physical map location of the argFGH operon of Escherichia coli. J Bacteriol. 1992;174:3836-7 pubmed
  6. Hendrickson W, Stoner C, Schleif R. Characterization of the Escherichia coli araFGH and araJ promoters. J Mol Biol. 1990;215:497-510 pubmed
    ..Two CRP sites were observed; one is upstream from the AraC site and one is downstream from the transcription start site. ..
  7. Horazdovsky B, Hogg R. High-affinity L-arabinose transport operon. Gene product expression and mRNAs. J Mol Biol. 1987;197:27-35 pubmed
    ..A 52,000 Mr protein is encoded by sequence 3' to araF and has been assigned to the araG locus. The sequence 3' to araG encodes a 31,000 Mr protein that has been assigned to the araH locus...
  8. Kolodrubetz D, Schleif R. L-arabinose transport systems in Escherichia coli K-12. J Bacteriol. 1981;148:472-9 pubmed
    ..system was found to contain two genes: araF, which codes for the arabinose-binding protein, and a new gene, araG. The newly identified gene, araG, was shown by two-dimensional gel electrophoresis to encode a protein which is ..
  9. Daruwalla K, Paxton A, Henderson P. Energization of the transport systems for arabinose and comparison with galactose transport in Escherichia coli. Biochem J. 1981;200:611-27 pubmed
    ..The properties of the two transport systems for arabinose are compared with those of the genetically and biochemically distinct transport systems for galactose, GalP and MglP. It appears that AraE is analogous to GalP, and AraF to MglP. ..

More Information

Publications13

  1. Kosiba B, Schleif R. Arabinose-inducible promoter from Escherichia coli. Its cloning from chromosomal DNA, identification as the araFG promoter and sequence. J Mol Biol. 1982;156:53-66 pubmed
  2. Scripture J, Voelker C, Miller S, O Donnell R, Polgar L, Rade J, et al. High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products. J Mol Biol. 1987;197:37-46 pubmed
    ..has been determined 3' from the regulatory region and found to contain three open reading frames designated araF, araG and araH...
  3. Horazdovsky B, Hogg R. Genetic reconstitution of the high-affinity L-arabinose transport system. J Bacteriol. 1989;171:3053-9 pubmed
    ..Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF).
  4. Kolodrubetz D, Schleif R. Regulation of the L-arabinose transport operons in Escherichia coli. J Mol Biol. 1981;151:215-27 pubmed