Gene Symbol: araF
Description: L-arabinose ABC transporter periplasmic binding protein
Alias: ECK1899, JW1889
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Quiocho F, Vyas N. Novel stereospecificity of the L-arabinose-binding protein. Nature. 1984;310:381-6 pubmed
  2. Clark A, Hogg R. High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location. J Bacteriol. 1981;147:920-4 pubmed
    The gene araF, the product of which is the L-arabinose-binding protein--a component of the high-affinity L-arabinose transport system, was located on the Escherichia coli linkage map at 45 min...
  3. Hogg R, Isihara H, Hermodson M, Koshland D, Jacobs J, Bradshaw R. A comparison of the amino-terminal sequences of several carbohydrate binding proteins from Escherichia coli and Salmonella typhimurium. FEBS Lett. 1977;80:377-9 pubmed
  4. Kehres D, Hogg R. Escherichia coli K12 arabinose-binding protein mutants with altered transport properties. Protein Sci. 1992;1:1652-60 pubmed
    ..The evidence suggests that this surface of the binding protein and these nine charged loci play a major role in ABP interactions with the membrane complex. ..
  5. Schleif R. An L-arabinose binding protein and arabinose permeation in Escherichia coli. J Mol Biol. 1969;46:185-96 pubmed
  6. Vermersch P, Tesmer J, Lemon D, Quiocho F. A Pro to Gly mutation in the hinge of the arabinose-binding protein enhances binding and alters specificity. Sugar-binding and crystallographic studies. J Biol Chem. 1990;265:16592-603 pubmed
    ..Stabilizing contacts are formed between the strands of the hinge in the Gly-254 ABP when galactose is bound which are not found in complexes with the other sugars or the liganded wild-type protein. ..
  7. Gryczynski U, Schleif R. A portable allosteric mechanism. Proteins. 2004;57:9-11 pubmed
    ..Arabinose, which regulates the position of the arm in AraC protein now regulates the availability of the alpha-peptide to alpha-acceptor beta-galactosidase, thereby modulating its activity in response to arabinose. ..
  8. Horazdovsky B, Hogg R. High-affinity L-arabinose transport operon. Gene product expression and mRNAs. J Mol Biol. 1987;197:27-35 pubmed a 33,000 Mr protein that is the product of the promoter-proximal L-arabinose binding protein coding sequence, araF. A 52,000 Mr protein is encoded by sequence 3' to araF and has been assigned to the araG locus...
  9. Soisson S, MacDougall Shackleton B, Schleif R, Wolberger C. The 1.6 A crystal structure of the AraC sugar-binding and dimerization domain complexed with D-fucose. J Mol Biol. 1997;273:226-37 pubmed

More Information


  1. Newcomer M, Gilliland G, Quiocho F. L-Arabinose-binding protein-sugar complex at 2.4 A resolution. Stereochemistry and evidence for a structural change. J Biol Chem. 1981;256:13213-7 pubmed
    ..Protein structural change accompanying binding is indicated by the inaccessibility of the bound L-arabinose to the aqueous environment. ..
  2. Kolodrubetz D, Schleif R. Regulation of the L-arabinose transport operons in Escherichia coli. J Mol Biol. 1981;151:215-27 pubmed
  3. Horazdovsky B, Hogg R. Genetic reconstitution of the high-affinity L-arabinose transport system. J Bacteriol. 1989;171:3053-9 pubmed
    ..Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF).
  4. Kosiba B, Schleif R. Arabinose-inducible promoter from Escherichia coli. Its cloning from chromosomal DNA, identification as the araFG promoter and sequence. J Mol Biol. 1982;156:53-66 pubmed
  5. Daruwalla K, Paxton A, Henderson P. Energization of the transport systems for arabinose and comparison with galactose transport in Escherichia coli. Biochem J. 1981;200:611-27 pubmed
    1. Strains of Escherichia coli were obtained containing either the AraE or the AraF transport system for arabinose...
  6. Kolodrubetz D, Schleif R. L-arabinose transport systems in Escherichia coli K-12. J Bacteriol. 1981;148:472-9 pubmed
    ..5 min) and closer to lysA. The operon of the high-affinity transport system was found to contain two genes: araF, which codes for the arabinose-binding protein, and a new gene, araG...
  7. Hendrickson W, Rudd K. Physical map location of the argFGH operon of Escherichia coli. J Bacteriol. 1992;174:3836-7 pubmed
  8. Hogg R. L-Arabinose transport and the L-arabinose binding protein of Escherichia coli. J Supramol Struct. 1977;6:411-7 pubmed
    ..The other, specified in part by the genetic locus araF, contains as a functional component the L-arabinose binding protein and functions with a "high affinity" ..
  9. MacRaild C, Daranas A, Bronowska A, Homans S. Global changes in local protein dynamics reduce the entropic cost of carbohydrate binding in the arabinose-binding protein. J Mol Biol. 2007;368:822-32 pubmed
    ..These results suggest that the structure and dynamics of ABP may be adapted to exploit dynamic changes to reduce the entropic costs of binding. ..
  10. Quiocho F, Gilliland G, Phillips G. The 2.8-A resolution structure of the L-arabinose-binding protein from Escherichia coli. Polypeptide chain folding, domain similarity, and probable location of sugar-binding site. J Biol Chem. 1977;252:5142-9 pubmed
  11. Scripture J, Hogg R. The nucleotide sequences defining the signal peptides of the galactose-binding protein and the arabinose-binding protein. J Biol Chem. 1983;258:10853-5 pubmed
    The nucleotide sequences of the NH2-terminal regions of the mglB (galactose-binding protein) and the araF (arabinose-binding protein) genes have been determined...
  12. Khlebnikov A, Risa O, Skaug T, Carrier T, Keasling J. Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture. J Bacteriol. 2000;182:7029-34 pubmed
    ..This work demonstrates the importance of including a transport gene that is controlled independently of the inducer to achieve regulatable and consistent induction in all cells of the culture. ..
  13. Hogg R, Hermodson M. Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r. J Biol Chem. 1977;252:5135-41 pubmed
    ..The COOH-terminal sequence was determined using bovine carboxypeptidases A and B and amino acid analyses. The L-arabinose-binding protein was determined to contain 306 amino acid residues, the sequence of which is presented below. ..
  14. Schreier B, Stumpp C, Wiesner S, Höcker B. Computational design of ligand binding is not a solved problem. Proc Natl Acad Sci U S A. 2009;106:18491-6 pubmed publisher
    ..Hence, the computational design of ligand binding is not a solved problem and needs to be revisited. ..
  15. Brown C, Hogg R. A second transport system for L-arabinose in Escherichia coli B-r controlled by the araC gene. J Bacteriol. 1972;111:606-13 pubmed
    ..These observations support a role for the binding protein in l-arabinose uptake. ..
  16. Scripture J, Voelker C, Miller S, O Donnell R, Polgar L, Rade J, et al. High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products. J Mol Biol. 1987;197:37-46 pubmed
    ..operon has been determined 3' from the regulatory region and found to contain three open reading frames designated araF, araG and araH...
  17. Hendrickson W, Stoner C, Schleif R. Characterization of the Escherichia coli araFGH and araJ promoters. J Mol Biol. 1990;215:497-510 pubmed
    ..Two CRP sites were observed; one is upstream from the AraC site and one is downstream from the transcription start site. ..
  18. Jojima T, Omumasaba C, Inui M, Yukawa H. Sugar transporters in efficient utilization of mixed sugar substrates: current knowledge and outlook. Appl Microbiol Biotechnol. 2010;85:471-80 pubmed publisher
    ..Subsequently, we review and discuss recent studies on engineering of sugar transport as a driving force for efficient bioconversion of sugar mixtures derived from lignocellulose. ..