araB

Summary

Gene Symbol: araB
Description: L-ribulokinase
Alias: ECK0064, JW0062
Species: Escherichia coli str. K-12 substr. MG1655
Products:     araB

Top Publications

  1. Gross J, Englesberg E. Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r bv transduction with phage Plbt. Virology. 1959;9:314-31 pubmed
  2. Lee N, Gielow W, Martin R, Hamilton E, Fowler A. The organization of the araBAD operon of Escherichia coli. Gene. 1986;47:231-44 pubmed
    ..The nt sequence predicts a transcript of about 4250 nt. The coding regions of araB, araA and araD genes were identified by partial amino acid (aa) sequences of the purified proteins and their ..
  3. Kaplan S, Schleif R. Excision of bacteriophage lambda from a site in the arabinose B gene. J Bacteriol. 1975;124:1403-10 pubmed
    ..The efficiency with which these phage transduce araC and araB point mutations was found to be approximately proportional to the homology length available for recombination.
  4. Lee L, Gerratana B, Cleland W. Substrate specificity and kinetic mechanism of Escherichia coli ribulokinase. Arch Biochem Biophys. 2001;396:219-24 pubmed
    ..These data show that the mechanism is random, but there is a high level of synergism in the binding of sugar and MgATP, and the path in which the sugar adds first is strongly preferred...
  5. Giffard P, Booth I. The rpoA341 allele of Escherichia coli specifically impairs the transcription of a group of positively-regulated operons. Mol Gen Genet. 1988;214:148-52 pubmed
    ..The significance of these observations is discussed in the context of models of positive regulation. ..
  6. Ogden S, Haggerty D, Stoner C, Kolodrubetz D, Schleif R. The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation. Proc Natl Acad Sci U S A. 1980;77:3346-50 pubmed
    ..Thus, from a single operator site, the negative regulator represses the two outwardly oriented ara promoters. This regulatory mechanism explains the known positive and negative regulatory properties of the ara promoters. ..
  7. Schleif R. L-arabinose operon messenger of Escherichia coli. Its inducibility and translation efficiency relative to lactose operon messenger. J Mol Biol. 1971;61:275-9 pubmed
  8. Wade J, Struhl K. Association of RNA polymerase with transcribed regions in Escherichia coli. Proc Natl Acad Sci U S A. 2004;101:17777-82 pubmed
    ..These observations suggest that in vivo association of sigma(70) and NusA with elongating RNAP is regulated by growth conditions. ..
  9. Lu C, Bentley W, Rao G. A high-throughput approach to promoter study using green fluorescent protein. Biotechnol Prog. 2004;20:1634-40 pubmed

More Information

Publications28

  1. Haldimann A, Daniels L, Wanner B. Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J Bacteriol. 1998;180:1277-86 pubmed
    ..and PhoR synthesis from their normal controls by placing each under the tight control of the arabinose-regulated P(araB) promoter or the rhamnose-regulated P(rhaB) promoter...
  2. Lee N, Bendet I. Crystalline L-ribulokinase from Escherichia coli. J Biol Chem. 1967;242:2043-50 pubmed
  3. Rosen H, Lewis P, Nitzel C. Neutrophil microbicidal activity: screening bacterial mutants for survival after phagocytosis using quantitative PCR. Jpn J Infect Dis. 2004;57:S19-21 pubmed
    ..describe Escherichia coli survival following neutrophil phagocytosis among three mutants deleted respectively for araB, dps or oxyR...
  4. Lee N, Gielow W, Wallace R. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli. Proc Natl Acad Sci U S A. 1981;78:752-6 pubmed
    ..An earlier model proposed by Ogden et al. [Ogden S., Haggerty, D., Stoner, C. M., Kolodrubetz, D. & Schleif, R. (1980) Proc. Natl. Acad. Sci, USA 77, 3346-3350] is discussed in the light of the data presented in this paper. ..
  5. Schleif R, Greenblatt J, Davis R. Dual control of arabinose genes on transducing phage lambda-dara. J Mol Biol. 1971;59:127-50 pubmed
  6. Lee D, Schleif R. In vivo DNA loops in araCBAD: size limits and helical repeat. Proc Natl Acad Sci U S A. 1989;86:476-80 pubmed
    ..As the spacing between araI and araO2 varied, the activity of PBAD oscillated with an 11.1-base-pair periodicity, implying that the in vivo helical repeat of this DNA is 11.1 base pairs per turn. ..
  7. Lee N, Carbon J. Nucleotide sequence of the 5' end of araBAD operon messenger RNA in Escherichia coli B/r. Proc Natl Acad Sci U S A. 1977;74:49-53 pubmed
    ..combined with the knowledge of the NH2-terminal sequence of the product of the first cistron (L-ribulokinase gene araB), establish the nucleotide sequence of the first 69 bases at the 5' end of the araBAD operon mRNA...
  8. Johnson C, Schleif R. In vivo induction kinetics of the arabinose promoters in Escherichia coli. J Bacteriol. 1995;177:3438-42 pubmed
    ..5, 5, and 1, respectively. These results provide a basis for subsequent studies to determine the mechanism(s) by which AraC protein activates transcription from the different arabinose promoters. ..
  9. Ebright R, Beckwith J. The catabolite gene activator protein (CAP) is not required for indole-3-acetic acid to activate transcription of the araBAD operon of Escherichia coli K-12. Mol Gen Genet. 1985;201:51-5 pubmed
    ..However, contrary to the results of Kline et al., we find that the action of IAA does not require CAP. Thus, IAA fully stimulates the transcription of araBAD in a strain which bears a complete deletion of the crp gene. ..
  10. Lee D, Huo L, Schleif R. Repression of the araBAD promoter from araO1. J Mol Biol. 1992;224:335-41 pubmed
  11. Casadaban M. Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions. Proc Natl Acad Sci U S A. 1975;72:809-13 pubmed
    ..These fusions extend the techniques available for studies on the lac operon to studies on the ara operon. It should be possible to fuse other operons by this method. ..
  12. Katz L. Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol. J Bacteriol. 1970;102:593-5 pubmed
    ..Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed.
  13. Lee N, Patrick J, Barnes N. Subunit structure of L-ribulokinase from Escherichia coli. J Biol Chem. 1970;245:1357-61 pubmed
  14. Dunn T, Schleif R. Deletion analysis of the Escherichia coli ara PC and PBAD promoters. J Mol Biol. 1984;180:201-4 pubmed
    ..Similarly, deletions entering this site from the PBAD side reduced activity of the PC promoter. Cyclic AMP receptor protein bound at this site apparently functions to stimulate transcription of both flanking promoters. ..
  15. Miyada C, Soberon X, Itakura K, Wilcox G. The use of synthetic oligodeoxyribonucleotides to produce specific deletions in the araBAD promoter of Escherichia coli B/r. Gene. 1982;17:167-77 pubmed
    ..Transcription studies performed on strains containing the mutant ara plasmids demonstrated that both mutations reduced the amount of araBA mRNA synthesized in the presence of L-arabinose. ..
  16. Guzman L, Belin D, Carson M, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995;177:4121-30 pubmed
    ..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system. ..
  17. Reeder T, Schleif R. Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon. J Bacteriol. 1991;173:7765-71 pubmed
    ..S. Naom, S. J. Morton, D. R. F. Leach, and R. G. Lloyd, Nucleic Acids Res. 17:8033-8044, 1989). The carboxyl region of SbcC has an amino acid sequence consistent with this region of SbcC forming an extended alpha-helical coiled-coil. ..
  18. Smith B, Schleif R. Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12. J Biol Chem. 1978;253:6931-3 pubmed
    ..This segment includes the promoter for rightward araBAD transcription, pBAD, the promoter for leftward araC transcription, pC, and sites responsible for repression. ..
  19. Ibanez E, Gimenez R, Pedraza T, Baldoma L, Aguilar J, Badia J. Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose. J Bacteriol. 2000;182:4625-7 pubmed
    ..Similarity of YiaR to several 3-epimerases suggested that this protein could catalyze the conversion of L-xylulose-5-phosphate into L-ribulose-5-phosphate, thus completing the pathway between L-xylulose and the general metabolism. ..