anaerobic C4 dicarboxylate transport
Gene Symbol: anaerobic C4 dicarboxylate transport
Description: anaerobic C4-dicarboxylate transport
Alias: ECK0614, JW0613, JW0616
Species: Escherichia coli str. K-12 substr. MG1655
Engel P, Kramer R, Unden G. Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from the aerobic dicarboxylate uptake system. J Bacteriol. 1992;174:5533-9 pubmed
..The inhibition is reversed by the presence of reducing agents. It is suggested that the physiological role of the system is a fumarate/succinate antiport under conditions of fumarate respiration. ..
Engel P, Kramer R, Unden G. Transport of C4-dicarboxylates by anaerobically grown Escherichia coli. Energetics and mechanism of exchange, uptake and efflux. Eur J Biochem. 1994;222:605-14 pubmed
..The efflux of succinate from de-energized membrane vesicles generated a delta psi of -70 mV. It is thus suggested that succinate efflux functions as a H+/succinate symport. ..
Zientz E, Janausch I, Six S, Unden G. Functioning of DcuC as the C4-dicarboxylate carrier during glucose fermentation by Escherichia coli. J Bacteriol. 1999;181:3716-20 pubmed
..Upon overexpression in a dcuC promoter mutant (dcuC*), DcuC was able to compensate for DcuA and DcuB in fumarate-succinate exchange and fumarate uptake. ..
Janausch -, Unden -. The dcuD (former yhcL) gene product of escherichia coli as a member of the DcuC family of C4-dicarboxylate carriers: lack of evident expression. Arch Microbiol. 1999;172:219-26 pubmed
..When two adjacent open reading frames (y0585 and y0586) from Haemophilus influenzae are fused, the resulting hypothetical protein has sequence similarity to DcuC and DcuD. ..
Janausch I, Kim O, Unden G. DctA- and Dcu-independent transport of succinate in Escherichia coli: contribution of diffusion and of alternative carriers. Arch Microbiol. 2001;176:224-30 pubmed
..In contrast, the efflux of succinate produced during glucose fermentation was not affected in the mutants, demonstrating that, for succinate efflux, a carrier different from, or in addition to, the known Dcu and CitT carriers is used. ..
Chen J, Zhu X, Tan Z, Xu H, Tang J, Xiao D, et al
. Activating C4-dicarboxylate transporters DcuB and DcuC for improving succinate production. Appl Microbiol Biotechnol. 2014;98:2197-205 pubmed publisher
..Modulating these two genes in combination led to 34% increase of succinate titer. To the best of knowledge, this was the first report about improving succinate production through engineering succinate efflux system. ..
Zientz E, Six S, Unden G. Identification of a third secondary carrier (DcuC) for anaerobic C4-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in uptake and exchange. J Bacteriol. 1996;178:7241-7 pubmed
..Mutants producing only DcuA, DcuB, or DcuC were prepared. In the mutants, DcuA, DcuB, and DcuC were each able to operate in the exchange and uptake mode. ..