amiB

Summary

Gene Symbol: amiB
Description: N-acetylmuramoyl-l-alanine amidase II
Alias: ECK4165, JW4127, yjeD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Beck B, Park J. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. J Bacteriol. 1976;126:1250-60 pubmed
  2. Tsui H, Winkler M. Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation. Biochimie. 1994;76:1168-77 pubmed
    The complex amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of E coli contains important genes for several fundamental cellular processes, including cell-wall hydrolysis (amiB), DNA repair (mutL), tRNA modification (miaA) and proteolysis (..
  3. Tsui H, Leung H, Winkler M. Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12. Mol Microbiol. 1994;13:35-49 pubmed
    ..Together, these results show that hfq function plays a fundamental role in Escherichia coli physiology and that hfq and the hflA-region are in the amiB-mutL-miaA-hfq-hflX superoperon.
  4. Tsui H, Feng G, Winkler M. Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered Esigma32-specific promoters during heat shock. J Bacteriol. 1996;178:5719-31 pubmed
    The amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of Escherichia coli contains genes that are important for diverse cellular functions, including DNA mismatch repair (mutL), tRNA modification (miaA), pleiotropic regulation (hfq), and ..
  5. Heidrich C, Templin M, Ursinus A, Merdanovic M, Berger J, Schwarz H, et al. Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli. Mol Microbiol. 2001;41:167-78 pubmed
    ..As amidase deletion mutants accumulate trimeric and tetrameric cross-links in their murein, it is suggested that these structures mark the division site before cleavage of the septum. ..
  6. Bernhardt T, de Boer P. The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathway. Mol Microbiol. 2003;48:1171-82 pubmed
    ..AmiC therefore replaces FtsN as the latest known recruit to the septal ring and is the first entirely periplasmic component to be localized. ..
  7. Ize B, Stanley N, Buchanan G, Palmer T. Role of the Escherichia coli Tat pathway in outer membrane integrity. Mol Microbiol. 2003;48:1183-93 pubmed
    ..This outer membrane repair of the tat mutant arises as a result of upregulation of the amiB gene, which encodes a cell wall amidase...
  8. Sikdar R, Doerrler W. Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes. J Bacteriol. 2010;192:807-18 pubmed publisher
    ..Overexpression of AmiA, AmiC, or AmiB, a periplasmic amidase secreted via the general secretory pathway, restores normal cell division but does not ..
  9. Yang D, Tan K, Joachimiak A, Bernhardt T. A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division. Mol Microbiol. 2012;85:768-81 pubmed publisher
    ..Here we investigate the control of cell division hydrolases called amidases (AmiA, AmiB and AmiC) required for Escherichia coli cell division...
  10. Tsui H, Mandavilli B, Winkler M. Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium. Nucleic Acids Res. 1992;20:2379 pubmed

Detail Information

Publications15

  1. Beck B, Park J. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. J Bacteriol. 1976;126:1250-60 pubmed
  2. Tsui H, Winkler M. Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation. Biochimie. 1994;76:1168-77 pubmed
    The complex amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of E coli contains important genes for several fundamental cellular processes, including cell-wall hydrolysis (amiB), DNA repair (mutL), tRNA modification (miaA) and proteolysis (..
  3. Tsui H, Leung H, Winkler M. Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12. Mol Microbiol. 1994;13:35-49 pubmed
    ..Together, these results show that hfq function plays a fundamental role in Escherichia coli physiology and that hfq and the hflA-region are in the amiB-mutL-miaA-hfq-hflX superoperon.
  4. Tsui H, Feng G, Winkler M. Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered Esigma32-specific promoters during heat shock. J Bacteriol. 1996;178:5719-31 pubmed
    The amiB-mutL-miaA-hfq-hflX-hflK-hflC superoperon of Escherichia coli contains genes that are important for diverse cellular functions, including DNA mismatch repair (mutL), tRNA modification (miaA), pleiotropic regulation (hfq), and ..
  5. Heidrich C, Templin M, Ursinus A, Merdanovic M, Berger J, Schwarz H, et al. Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli. Mol Microbiol. 2001;41:167-78 pubmed
    ..As amidase deletion mutants accumulate trimeric and tetrameric cross-links in their murein, it is suggested that these structures mark the division site before cleavage of the septum. ..
  6. Bernhardt T, de Boer P. The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathway. Mol Microbiol. 2003;48:1171-82 pubmed
    ..AmiC therefore replaces FtsN as the latest known recruit to the septal ring and is the first entirely periplasmic component to be localized. ..
  7. Ize B, Stanley N, Buchanan G, Palmer T. Role of the Escherichia coli Tat pathway in outer membrane integrity. Mol Microbiol. 2003;48:1183-93 pubmed
    ..This outer membrane repair of the tat mutant arises as a result of upregulation of the amiB gene, which encodes a cell wall amidase...
  8. Sikdar R, Doerrler W. Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes. J Bacteriol. 2010;192:807-18 pubmed publisher
    ..Overexpression of AmiA, AmiC, or AmiB, a periplasmic amidase secreted via the general secretory pathway, restores normal cell division but does not ..
  9. Yang D, Tan K, Joachimiak A, Bernhardt T. A conformational switch controls cell wall-remodelling enzymes required for bacterial cell division. Mol Microbiol. 2012;85:768-81 pubmed publisher
    ..Here we investigate the control of cell division hydrolases called amidases (AmiA, AmiB and AmiC) required for Escherichia coli cell division...
  10. Tsui H, Mandavilli B, Winkler M. Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium. Nucleic Acids Res. 1992;20:2379 pubmed
  11. Tsui H, Zhao G, Feng G, Leung H, Winkler M. The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase. Mol Microbiol. 1994;11:189-202 pubmed
    ..Insertions in this upstream gene, which we named amiB, or in mutL did not affect cell shape or viability; however, overexpression of the AmiB polypeptide caused cell ..
  12. Kitagawa R, Mitsuki H, Okazaki T, Ogawa T. A novel DnaA protein-binding site at 94.7 min on the Escherichia coli chromosome. Mol Microbiol. 1996;19:1137-47 pubmed
    ..is a DNA sequence which has unusually high affinity for the DnaA protein of Escherichia coli between the glyV and amiB-mutL operons at 94.7 min on the genetic map...
  13. Priyadarshini R, de Pedro M, Young K. Role of peptidoglycan amidases in the development and morphology of the division septum in Escherichia coli. J Bacteriol. 2007;189:5334-47 pubmed
  14. Uehara T, Dinh T, Bernhardt T. LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli. J Bacteriol. 2009;191:5094-107 pubmed publisher
    ..factors bear a striking resemblance to those of mutants defective for the N-acetylmuramyl-l-alanine amidases: AmiA, AmiB, and AmiC. E...
  15. Peters N, Dinh T, Bernhardt T. A fail-safe mechanism in the septal ring assembly pathway generated by the sequential recruitment of cell separation amidases and their activators. J Bacteriol. 2011;193:4973-83 pubmed publisher
    ..Septal PG splitting is mediated by the amidases: AmiA, AmiB, and AmiC. To efficiently hydrolyze PG, the amidases must be activated by LytM domain factors...