Gene Symbol: amiA
Description: N-acetylmuramoyl-l-alanine amidase I
Alias: ECK2430, JW2428, yfeE
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Troup B, Jahn M, Hungerer C, Jahn D. Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant. J Bacteriol. 1994;176:673-80 pubmed
    ..It encodes a protein of 40.2 kDa that showed significant homology to proteins of the XylS/AraC family of transcriptional regulators. ..
  2. Chung H, Yao Z, Goehring N, Kishony R, Beckwith J, Kahne D. Rapid beta-lactam-induced lysis requires successful assembly of the cell division machinery. Proc Natl Acad Sci U S A. 2009;106:21872-7 pubmed publisher
    ..One seemingly paradoxical implication of our results is that beta-lactam therapy may be improved by promoting active cell division. ..
  3. Uehara T, Dinh T, Bernhardt T. LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli. J Bacteriol. 2009;191:5094-107 pubmed publisher
    ..factors bear a striking resemblance to those of mutants defective for the N-acetylmuramyl-l-alanine amidases: AmiA, AmiB, and AmiC. E...
  4. Heidrich C, Ursinus A, Berger J, Schwarz H, Höltje J. Effects of multiple deletions of murein hydrolases on viability, septum cleavage, and sensitivity to large toxic molecules in Escherichia coli. J Bacteriol. 2002;184:6093-9 pubmed
    ..coli. ..
  5. van Heijenoort J, Parquet C, Flouret B, van Heijenoort Y. Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme. Eur J Biochem. 1975;58:611-9 pubmed
    ..Further characterisation of this N-acetylmuramyl-L-alanine amidase activity was carried out by investigating several of its properties. ..
  6. Narita S, Taketani S, Inokuchi H. Oxidation of protoporphyrinogen IX in Escherichia coli is mediated by the aerobic coproporphyrinogen oxidase. Mol Gen Genet. 1999;261:1012-20 pubmed
    ..These findings suggest that the E. coli aerobic coproporphyrinogen oxidase has an intrinsic capacity to oxidize not only coproporphyrinogen III but also protoporphyrinogen IX. ..
  7. Lupoli T, Taniguchi T, Wang T, Perlstein D, Walker S, KAHNE D. Studying a cell division amidase using defined peptidoglycan substrates. J Am Chem Soc. 2009;131:18230-1 pubmed publisher
    ..the catalytic activity and substrate specificity of the important Escherichia coli cell division amidase AmiA. We show that AmiA is a zinc metalloprotease that requires at least a tetrasaccharide glycopeptide substrate for ..
  8. Vanderwinkel E, de Vlieghere M. Modulation of Escherichia coli N-acetylmuramoyl-L-alanine amidase activity by phosphatidylglycerol. Biochim Biophys Acta. 1985;838:54-9 pubmed
    ..Possible physiological meaning of the results is briefly discussed in the context of our work and that done previously by others. ..
  9. Bernhardt T, de Boer P. The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathway. Mol Microbiol. 2003;48:1171-82 pubmed
    The N-acetylmuramoyl-l-alanine amidases of Escherichia coli (AmiA, B and C) are periplasmic enzymes that remove murein cross-links by cleaving the peptide moiety from N-acetylmuramic acid...

More Information


  1. Sikdar R, Doerrler W. Inefficient Tat-dependent export of periplasmic amidases in an Escherichia coli strain with mutations in two DedA family genes. J Bacteriol. 2010;192:807-18 pubmed publisher
    ..Periplasmic amidases AmiC and AmiA, secreted by the twin arginine transport (Tat) pathway, are localized to the cytoplasm in BC202...
  2. Beck B, Park J. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. J Bacteriol. 1976;126:1250-60 pubmed
  3. Tomioka S, Nikaido T, Miyakawa T, Matsuhashi M. Mutation of the N-acetylmuramyl-L-alanine amidase gene of Escherichia coli K-12. J Bacteriol. 1983;156:463-5 pubmed
    Mutants of Escherichia coli with very low N-acetylmuramyl-L-alanine amidase activity were isolated. The gene amiA responsible for most of this enzyme activity was mapped at 51 min on the E...
  4. Priyadarshini R, de Pedro M, Young K. Role of peptidoglycan amidases in the development and morphology of the division septum in Escherichia coli. J Bacteriol. 2007;189:5334-47 pubmed
  5. Heidrich C, Templin M, Ursinus A, Merdanovic M, Berger J, Schwarz H, et al. Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli. Mol Microbiol. 2001;41:167-78 pubmed
    ..Here, we report that three amidases, named AmiA, B and C, exist in E. coli and that they are involved in splitting of the murein septum during cell division...
  6. Ize B, Stanley N, Buchanan G, Palmer T. Role of the Escherichia coli Tat pathway in outer membrane integrity. Mol Microbiol. 2003;48:1183-93 pubmed
    ..Overexpression of the genes encoding the two additional amidases, amiA and amiC, does not compensate for the outer membrane defect of the tatC strain...
  7. Tanaka K, Muramatsu S, Yamada H, Mizuno T. Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance. Mol Gen Genet. 1991;226:367-76 pubmed
    ..It was also demonstrated that an E. coli histone-like protein, named H-NS (or H1a), exhibits a strong affinity for naturally occurring curved DNA sequences in regions upstream promoters. ..
  8. Vanderwinkel E, de Vlieghere M, Charles P, Baptist V. Nature of the interactions involved in the lipid-protein complexes of the Escherichia coli N-acetylmuramoyl-L-alanine amidase. Biochim Biophys Acta. 1987;913:238-44 pubmed
    ..All kinetic data supported our previous interpretation of the phosphatidylglycerol-enzyme interactions in terms of multisite non-allosteric theory. ..
  9. Uehara T, Park J. Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation. J Bacteriol. 2008;190:3914-22 pubmed publisher