Gene Symbol: ahpC
Description: alkyl hydroperoxide reductase, C22 subunit
Alias: ECK0599, JW0598, tpx
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Ueshima R, Fujita N, Ishihama A. Identification of Escherichia coli proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit. Biochem Biophys Res Commun. 1992;184:634-9 pubmed
    ..2 peptide indicated that the anti-sigma peptide serum contained at least three different species of antibodies. All the four SCRPs analyzed here reacted with an antibody against a C-terminus-proximal epitope. ..
  2. Fredriksson A, Nystrom T. Conditional and replicative senescence in Escherichia coli. Curr Opin Microbiol. 2006;9:612-8 pubmed
    ..Thus, bacterial physiology might entail both conditional and mandatory aging processes. ..
  3. Poole L. Bacterial defenses against oxidants: mechanistic features of cysteine-based peroxidases and their flavoprotein reductases. Arch Biochem Biophys. 2005;433:240-54 pubmed
    ..express a flavoprotein, AhpF, which acts as a dedicated disulfide reductase to recycle the bacterial peroxiredoxin, AhpC, during catalysis...
  4. Seaver L, Imlay J. Hydrogen peroxide fluxes and compartmentalization inside growing Escherichia coli. J Bacteriol. 2001;183:7182-9 pubmed
  5. Zheng M, Wang X, Doan B, Lewis K, Schneider T, Storz G. Computation-directed identification of OxyR DNA binding sites in Escherichia coli. J Bacteriol. 2001;183:4571-9 pubmed
    ..dsbG gene and 238 bp upstream of a known OxyR binding site in the promoter region of the divergently transcribed ahpC gene...
  6. Blankenhorn D, Phillips J, Slonczewski J. Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J Bacteriol. 1999;181:2209-16 pubmed
    ..Acid or propionate also increased the expression of alkyl hydroperoxide reductase (AhpC) but only during aerobic growth...
  7. Poole L, Ellis H. Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry. 1996;35:56-64 pubmed
    The two components, AhpF and AhpC, of the Salmonella typhimurium alkyl hydroperoxide reductase enzyme system have been overexpressed and purified from Escherichia coli for investigations of their catalytic properties...
  8. Tsuji K, Copeland N, Jenkins N, Obinata M. Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli. Biochem J. 1995;307 ( Pt 2):377-81 pubmed
    ..Sequence comparisons with recently cloned mammalian Aop1 homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation and antioxidant functions. ..
  9. Ferrante A, Augliera J, Lewis K, Klibanov A. Cloning of an organic solvent-resistance gene in Escherichia coli: the unexpected role of alkylhydroperoxide reductase. Proc Natl Acad Sci U S A. 1995;92:7617-21 pubmed
    ..The mutation was localized to a substitution of valine for glycine at position 142 in the coding region of ahpC, which is the gene encoding the catalytic subunit of the enzyme...

More Information


  1. Cha M, Kim H, Kim I. Thioredoxin-linked "thiol peroxidase" from periplasmic space of Escherichia coli. J Biol Chem. 1995;270:28635-41 pubmed
    ..that the 24-kDa protein is an intact form of the 22-kDa protein that was previously identified 22-kDa subunit (AhpC) of E. coli alkyl hydroperoxide reductase (AhpC/AhpF). We isolated and sequenced an E...
  2. Storz G, Jacobson F, Tartaglia L, Morgan R, Silveira L, Ames B. An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli: genetic characterization and cloning of ahp. J Bacteriol. 1989;171:2049-55 pubmed
    ..A cis-acting element required for oxyR-dependent, rpoH-independent heat shock induction of the F52a protein was present at the S. typhimurium but not the E. coli ahp locus. ..
  3. Li Calzi M, Poole L. Requirement for the two AhpF cystine disulfide centers in catalysis of peroxide reduction by alkyl hydroperoxide reductase. Biochemistry. 1997;36:13357-64 pubmed
    ..hydroperoxide reductase component which transfers electrons from pyridine nucleotides to the peroxidase protein, AhpC, possesses two redox-active disulfide centers in addition to one FAD per subunit; the primary goal of these studies ..
  4. Garbe T, Kobayashi M, Yukawa H. Indole-inducible proteins in bacteria suggest membrane and oxidant toxicity. Arch Microbiol. 2000;173:78-82 pubmed
    Oxidant toxicity of indole was demonstrated by the induction of alkylhydroperoxide reductase subunit C (AhpC) in Escherichia coli K12 and by the constitutive overproduction of AhpC in a variant of E...
  5. Poole L, Godzik A, Nayeem A, Schmitt J. AhpF can be dissected into two functional units: tandem repeats of two thioredoxin-like folds in the N-terminus mediate electron transfer from the thioredoxin reductase-like C-terminus to AhpC. Biochemistry. 2000;39:6602-15 pubmed
    ..reductase system, catalyzes the NADH-dependent reduction of an active-site disulfide bond in the other component, AhpC, which in turn reduces hydroperoxide substrates...
  6. Faulkner M, Veeravalli K, Gon S, Georgiou G, Beckwith J. Functional plasticity of a peroxidase allows evolution of diverse disulfide-reducing pathways. Proc Natl Acad Sci U S A. 2008;105:6735-40 pubmed publisher
    ..Growth of Deltagor DeltatrxB strains is restored by a mutant (ahpC*) of the peroxiredoxin AhpC, converting it to a disulfide reductase that generates reduced glutathione...
  7. Jacobson F, Morgan R, Christman M, Ames B. An alkyl hydroperoxide reductase from Salmonella typhimurium involved in the defense of DNA against oxidative damage. Purification and properties. J Biol Chem. 1989;264:1488-96 pubmed
    ..This enzyme may thus serve as a prokaryotic equivalent to the glutathione reductase/glutathione peroxidase system in eukaryotes. ..
  8. Dip P, Kamariah N, Nartey W, Beushausen C, Kostyuchenko V, Ng T, et al. Key roles of the Escherichia coli AhpC C-terminus in assembly and catalysis of alkylhydroperoxide reductase, an enzyme essential for the alleviation of oxidative stress. Biochim Biophys Acta. 2014;1837:1932-43 pubmed
    ..AhpR) is a prototype of the Prxs-family, and is composed of an NADH-dependent AhpF reductase (57 kDa) and AhpC (21 kDa), catalyzing the reduction of H2O2. We show that the E...
  9. Ma L, Payne S. AhpC is required for optimal production of enterobactin by Escherichia coli. J Bacteriol. 2012;194:6748-57 pubmed publisher
    Escherichia coli alkyl hydroperoxide reductase subunit C (AhpC) is a peroxiredoxin that detoxifies peroxides. Here we show an additional role for AhpC in cellular iron metabolism of E. coli...
  10. Malesevic M, Poehlmann A, Hernandez Alvarez B, Diessner A, Träger M, Rahfeld J, et al. The protein-free IANUS peptide array uncovers interaction sites between Escherichia coli parvulin 10 and alkyl hydroperoxide reductase. Biochemistry. 2010;49:8626-35 pubmed publisher
    ..prolyl cis/trans isomerase (PPIase), physically interacts with the alkyl hydroperoxide reductase subunit C (AhpC) in bacterial cell extracts, as determined by affinity chromatography and chemical cross-linking experiments...
  11. Dip P, Kamariah N, Subramanian Manimekalai M, Nartey W, Balakrishna A, Eisenhaber F, et al. Structure, mechanism and ensemble formation of the alkylhydroperoxide reductase subunits AhpC and AhpF from Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2014;70:2848-62 pubmed publisher
    ..mechanistic model of AhpR as a hydroperoxide scavenger, in which the dimeric, extended AhpF prefers complex formation with the AhpC ring to accelerate the catalytic activity and thus to increase the chance of rescuing the cell from ROS.
  12. Campos E, Montella C, Garces F, Baldoma L, Aguilar J, Badia J. Aerobic L-ascorbate metabolism and associated oxidative stress in Escherichia coli. Microbiology. 2007;153:3399-408 pubmed
    ..Proteomic analysis also indicated enhanced expression of the alkyl hydroperoxide reductase encoded by the ahpC gene, suggesting a response to oxidative stress generated during the aerobic metabolism of l-ascorbate...
  13. Smillie D, Hayward R, Suzuki T, Fujita N, Ishihama A. Locations of genes encoding alkyl hydroperoxide reductase on the physical map of the Escherichia coli K-12 genome. J Bacteriol. 1992;174:3826-7 pubmed
  14. Park S, You X, Imlay J. Substantial DNA damage from submicromolar intracellular hydrogen peroxide detected in Hpx- mutants of Escherichia coli. Proc Natl Acad Sci U S A. 2005;102:9317-22 pubmed
    ..The rate constant of the Fenton reaction measured at physiological pH was much higher than under the acidic conditions that were used to determine the commonly cited value. ..
  15. Seaver L, Imlay J. Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. J Bacteriol. 2001;183:7173-81 pubmed
    ..In sum, E. coli does indeed generate substantial H(2)O(2), but damage is averted by the scavenging activity of Ahp. ..
  16. Moreau P, Gerard F, Lutz N, Cozzone P. Non-growing Escherichia coli cells starved for glucose or phosphate use different mechanisms to survive oxidative stress. Mol Microbiol. 2001;39:1048-60 pubmed
    ..In stark contrast, the inactivation of both oxyR and rpoS genes dramatically decreased the viability of glucose-starved cells. ..
  17. Bieger B, Essen L. Crystallization and preliminary X-ray analysis of the catalytic core of the alkylhydroperoxide reductase component AhpF from Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2000;56:92-4 pubmed
    ..nucleotide-disulfide oxidoreductases and channels electrons from NAD(P)H via a series of disulfides towards the AhpC component, which finally reduces the hydro-peroxide substrates...
  18. Cha M, Kim H, Kim I. Mutation and Mutagenesis of thiol peroxidase of Escherichia coli and a new type of thiol peroxidase family. J Bacteriol. 1996;178:5610-4 pubmed
    ..In an attempt to perform physiological and biochemical characterizations of the thiol Px, a thiol Px null (tpx) mutant and a functional-residue mutant of thiol Px were produced...
  19. Quadroni M, Staudenmann W, Kertesz M, James P. Analysis of global responses by protein and peptide fingerprinting of proteins isolated by two-dimensional gel electrophoresis. Application to the sulfate-starvation response of Escherichia coli. Eur J Biochem. 1996;239:773-81 pubmed
    ..N-terminal sequencing however, takes about 5 h/protein and approximately 1 pmol to obtain a 10 amino acid sequence for a search. ..
  20. Tartaglia L, Storz G, Ames B. Identification and molecular analysis of oxyR-regulated promoters important for the bacterial adaptation to oxidative stress. J Mol Biol. 1989;210:709-19 pubmed
    ..coli katG gene (encoding catalase). A set of ahpC promoter deletions was constructed in vitro and analysis of these deletions revealed the location of sequences that ..
  21. Kamariah N, Eisenhaber B, Eisenhaber F, Grüber G. Essential role of the flexible linker on the conformational equilibrium of bacterial peroxiredoxin reductase for effective regeneration of peroxiredoxin. J Biol Chem. 2017;292:6667-6679 pubmed publisher
    ..catalyzes the rapid reduction of the redox-active disulfide center of the antioxidant protein peroxiredoxin (AhpC) to detoxify ROS such as hydrogen peroxide, organic hydroperoxide, and peroxynitrite...