adhE

Summary

Gene Symbol: adhE
Description: fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase
Alias: ECK1235, JW1228, adhC, ana
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Kessler D, Herth W, Knappe J. Ultrastructure and pyruvate formate-lyase radical quenching property of the multienzymic AdhE protein of Escherichia coli. J Biol Chem. 1992;267:18073-9 pubmed
    The AdhE protein of Escherichia coli is a homopolymer of 96-kDa subunits harboring three Fe(2+)-dependent catalytic functions: acetaldehyde-CoA dehydrogenase, alcohol dehydrogenase, and pyruvate formatelyase (PFL) deactivase...
  2. Gupta S, Mat Jan F, Latifi M, Clark D. Acetaldehyde dehydrogenase activity of the AdhE protein of Escherichia coli is inhibited by intermediates in ubiquinone synthesis. FEMS Microbiol Lett. 2000;182:51-5 pubmed
    ..result in the inactivation of the coenzyme A-linked acetaldehyde dehydrogenase activity of the multifunctional AdhE protein of Escherichia coli. This activity is restored by addition of ubiquinone-0 to cell extracts...
  3. Danchin A, Krin E. Filling the gap between hns and adhE in Escherichia coli K12. Microbiology. 1995;141 ( Pt 4):959-60 pubmed
    ..We report the sequence of the hns-adh region, at 27 min on the genome. ..
  4. Zhu J, Shimizu K. Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition. Metab Eng. 2005;7:104-15 pubmed
    The effects of several single-gene knockout mutants (pykF, ppc, pflA, pta, and adhE mutants) on the metabolic flux distribution in Escherichia coli were investigated under microaerobic condition...
  5. Shone C, Fromm H. Steady-state and pre-steady-state kinetics of coenzyme A linked aldehyde dehydrogenase from Escherichia coli. Biochemistry. 1981;20:7494-501 pubmed
    ..A model incorporating two binding sites for NAD+ has been put forward to explain these observations. ..
  6. Gutheil W, Holmquist B, Vallee B. Purification, characterization, and partial sequence of the glutathione-dependent formaldehyde dehydrogenase from Escherichia coli: a class III alcohol dehydrogenase. Biochemistry. 1992;31:475-81 pubmed
    ..coli enzyme is closely related to the ancestor of the plant and animal dimeric zinc alcohol dehydrogenases. ..
  7. Matuszewska E, Kwiatkowska J, Ratajczak E, Kuczynska Wisnik D, Laskowska E. Role of Escherichia coli heat shock proteins IbpA and IbpB in protection of alcohol dehydrogenase AdhE against heat inactivation in the presence of oxygen. Acta Biochim Pol. 2009;56:55-61 pubmed
    ..In this report we followed the appearance and removal of aggregated alcohol dehydrogenase, AdhE, in E. coli submitted to heat stress in the presence of oxygen...
  8. Jourdan S, McDowall K. Sensing of 5' monophosphate by Escherichia coli RNase G can significantly enhance association with RNA and stimulate the decay of functional mRNA transcripts in vivo. Mol Microbiol. 2008;67:102-15 pubmed
  9. Acebron S, Martín I, Del Castillo U, Moro F, Muga A. DnaK-mediated association of ClpB to protein aggregates. A bichaperone network at the aggregate surface. FEBS Lett. 2009;583:2991-6 pubmed publisher
    ..After initial DnaJ binding, the cochaperone drives association of DnaK to aggregates, and in the third step, as shown here, DnaK mediates ClpB interaction with the aggregate surface. ..

More Information

Publications46

  1. Shimada T, Fujita N, Maeda M, Ishihama A. Systematic search for the Cra-binding promoters using genomic SELEX system. Genes Cells. 2005;10:907-18 pubmed
  2. Yong T, Li E, Clark D, Stanley S. Complementation of an Escherichia coli adhE mutant by the Entamoeba histolytica EhADH2 gene provides a method for the identification of new antiamebic drugs. Proc Natl Acad Sci U S A. 1996;93:6464-9 pubmed
    ..member of a newly defined family of prokaryotic multifunctional enzymes, which includes the Escherichia coli AdhE enzyme, an enzyme required for anaerobic growth of E. coli...
  3. Kessler D, Leibrecht I, Knappe J. Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE. FEBS Lett. 1991;281:59-63 pubmed
    ..This revealed the identity of PFL-deactivase with the alcohol and acetaldehyde dehydrogenases of E. coli. The multienzyme represents a homopolymeric protein (approximately 40 x 96 kDa) requiring Fe2+ for all functions. ..
  4. Cunningham P, Clark D. The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol Gen Genet. 1986;205:487-93 pubmed
    ..of mutants, those lacking acetaldehyde dehydrogenase alone or lacking both enzymes, mapped close to the regulatory adhC gene at 27 min on the E. coli genetic map...
  5. Nnyepi M, Peng Y, Broderick J. Inactivation of E. coli pyruvate formate-lyase: role of AdhE and small molecules. Arch Biochem Biophys. 2007;459:1-9 pubmed
    Escherichia coli AdhE has been reported to harbor three distinct enzymatic activities: alcohol dehydrogenase, acetaldehyde-CoA dehydrogenase, and pyruvate formate-lyase (PFL) deactivase...
  6. Leonardo M, Cunningham P, Clark D. Anaerobic regulation of the adhE gene, encoding the fermentative alcohol dehydrogenase of Escherichia coli. J Bacteriol. 1993;175:870-8 pubmed
    The regulation of the adhE gene, which encodes the trifunctional fermentative acetaldehyde-alcohol dehydrogenase of Escherichia coli, was investigated by the construction of gene fusions and by two-dimensional protein gel electrophoresis...
  7. Singh R, Yang Y, Lu B, Bennett G, San K. Expression of the pfl gene and resulting metabolite flux distribution in nuo and ackA-pta E. coli mutant strains. Biotechnol Prog. 2006;22:898-902 pubmed
    ..Real-time PCR demonstrated that the adhE gene expression in the ack-pta mutant strain was approximately 100 fold that of the same gene in the ackA-pta nuo ..
  8. Lorowitz W, Clark D. Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase. J Bacteriol. 1982;152:935-8 pubmed
    ..These mutations, designated adhE, are all located at the previously described adh regulatory locus...
  9. Becker S, Vlad D, Schuster S, Pfeiffer P, Unden G. Regulatory O2 tensions for the synthesis of fermentation products in Escherichia coli and relation to aerobic respiration. Arch Microbiol. 1997;168:290-6 pubmed
    ..2-0.4 mbar for ethanol, acetate, and succinate, and 1 mbar for formate. The pO0.5 for the expression of the adhE gene encoding alcohol dehydrogenase was approximately 0.8 mbar...
  10. Chen Y, Lin E. Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli. J Bacteriol. 1991;173:8009-13 pubmed
    The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various phi (adhE'-lacZ) adhE+ merodiploid strains...
  11. Aristarkhov A, Mikulskis A, Belasco J, Lin E. Translation of the adhE transcript to produce ethanol dehydrogenase requires RNase III cleavage in Escherichia coli. J Bacteriol. 1996;178:4327-32 pubmed
    Previous studies have shown that the adhE gene, which encodes a multifunctional protein with ethanol dehydrogenase activity, is under transcriptional regulation...
  12. Matuszewska E, Kwiatkowska J, Kuczynska Wisnik D, Laskowska E. Escherichia coli heat-shock proteins IbpA/B are involved in resistance to oxidative stress induced by copper. Microbiology. 2008;154:1739-47 pubmed publisher
    ..This indicates that IbpA/B protect Escherichia coli cells from oxidative damage caused by copper. We show that AdhE, one of the proteins exposed to oxidation, is protected by IbpA/B against copper-mediated inactivation both in vivo ..
  13. Bianco C, Imperlini E, Calogero R, Senatore B, Pucci P, Defez R. Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli. Microbiology. 2006;152:2421-31 pubmed
    ..and amino acid biosynthesis (leucine, isoleucine, valine and proline) were up-regulated, whereas the fermentative adhE gene was down-regulated...
  14. Goodlove P, Cunningham P, Parker J, Clark D. Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli. Gene. 1989;85:209-14 pubmed
    A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene (adhE) of Escherichia coli, was cloned into a multicopy vector...
  15. Clark D. The fermentation pathways of Escherichia coli. FEMS Microbiol Rev. 1989;5:223-34 pubmed
    ..The enzymes and corresponding genes involved in these fermentation pathways are described. The regulatory responses of these genes and enzymes are known but the details of the underlying regulatory mechanisms are still obscure. ..
  16. Yamato M, Takahashi Y, Tomotake H, Ota F, Hirota K, Yamaguchi K. Monoclonal antibodies to spirosin of Yersinia enterocolitica and analysis of the localization of spirosome by use of them. Microbiol Immunol. 1994;38:177-82 pubmed
    ..By cellular fractionation of YE72 and subsequent EIA and Western blot analysis, spirosome was shown to be present in the cytoplasm of YE72. ..
  17. Holland Staley C, Lee K, Clark D, Cunningham P. Aerobic activity of Escherichia coli alcohol dehydrogenase is determined by a single amino acid. J Bacteriol. 2000;182:6049-54 pubmed
    Expression of the alcohol dehydrogenase gene, adhE, in Escherichia coli is anaerobically regulated at both the transcriptional and the translational levels...
  18. Membrillo Hernandez J, Lin E. Regulation of expression of the adhE gene, encoding ethanol oxidoreductase in Escherichia coli: transcription from a downstream promoter and regulation by fnr and RpoS. J Bacteriol. 1999;181:7571-9 pubmed
    The adhE gene of Escherichia coli, located at min 27 on the chromosome, encodes the bifunctional NAD-linked oxidoreductase responsible for the conversion of acetyl-coenzyme A to ethanol during fermentative growth...
  19. Wachi M, Kaga N, Umitsuki G, Clark D, Nagai K. A novel RNase G mutant that is defective in degradation of adhE mRNA but proficient in the processing of 16S rRNA precursor. Biochem Biophys Res Commun. 2001;289:1301-6 pubmed
    ..RNase G, encoded by the rng gene, is involved in both the processing of 16S rRNA precursor and the degradation of adhE mRNA. Consequently, defects in RNase G result in elevation of AdhE levels...
  20. Membrillo Hernandez J, Echave P, Cabiscol E, Tamarit J, Ros J, Lin E. Evolution of the adhE gene product of Escherichia coli from a functional reductase to a dehydrogenase. Genetic and biochemical studies of the mutant proteins. J Biol Chem. 2000;275:33869-75 pubmed
    The multifunctional AdhE protein of Escherichia coli (encoded by the adhE gene) physiologically catalyzes the sequential reduction of acetyl-CoA to acetaldehyde and then to ethanol under fermentative conditions...
  21. Kaga N, Umitsuki G, Clark D, Nagai K, Wachi M. Extensive overproduction of the AdhE protein by rng mutations depends on mutations in the cra gene or in the Cra-box of the adhE promoter. Biochem Biophys Res Commun. 2002;295:92-7 pubmed
    Escherichia coli RNase G encoded by the rng gene is involved in degradation of adhE mRNA. Overproduction of the AdhE protein by rng mutants was found to depend on the genetic background of strains derived from DC272 (adhC81) or MC1061...
  22. Utrilla J, Gosset G, Martinez A. ATP limitation in a pyruvate formate lyase mutant of Escherichia coli MG1655 increases glycolytic flux to D-lactate. J Ind Microbiol Biotechnol. 2009;36:1057-62 pubmed publisher
    A derivative strain of Escherichia coli MG1655 for D-lactate production was constructed by deleting the pflB, adhE and frdA genes; this strain was designated "CL3...
  23. Yun N, San K, Bennett G. Enhancement of lactate and succinate formation in adhE or pta-ackA mutants of NADH dehydrogenase-deficient Escherichia coli. J Appl Microbiol. 2005;99:1404-12 pubmed
    ..Removal of ethanol production by adhE gene inactivation generated higher production of succinate and D-lactate...
  24. Leonardo M, Clark D. Locations of genes in the nar-adhE region of the Escherichia coli K-12 chromosome. J Bacteriol. 1991;173:1574-5 pubmed
  25. Jantama K, Zhang X, Moore J, Shanmugam K, Svoronos S, Ingram L. Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C. Biotechnol Bioeng. 2008;101:881-93 pubmed publisher
    ..by combining the deletion of genes that disrupt fermentation pathways for alternative products (ldhA::FRT, adhE::FRT, ackA::FRT, focA-pflB::FRT, mgsA, poxB) with growth-based selection for increased ATP production...
  26. Echave P, Tamarit J, Cabiscol E, Ros J. Novel antioxidant role of alcohol dehydrogenase E from Escherichia coli. J Biol Chem. 2003;278:30193-8 pubmed publisher
    Alcohol dehydrogenase E (AdhE) is an Fe-enzyme that, under anaerobic conditions, is involved in dissimilation of glucose...
  27. Sanchez A, Bennett G, San K. Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity. Metab Eng. 2005;7:229-39 pubmed
    ..A recombinant E. coli strain, SBS550MG, was created by deactivating adhE, ldhA and ack-pta from the central metabolic pathway and by activating the glyoxylate pathway through the ..
  28. Kim Y, Park J, Cho J, Cho K, Park Y, Lee J. Proteomic response analysis of a threonine-overproducing mutant of Escherichia coli. Biochem J. 2004;381:823-9 pubmed
    ..coli mutant. ..
  29. Knappe J, Sawers G. A radical-chemical route to acetyl-CoA: the anaerobically induced pyruvate formate-lyase system of Escherichia coli. FEMS Microbiol Rev. 1990;6:383-98 pubmed
    ..In contrast, the expression of the gene encoding the first iron-dependent converter enzyme is unaffected by anaerobiosis and is independent of the Fnr protein. ..
  30. Mikulskis A, Aristarkhov A, Lin E. Regulation of expression of the ethanol dehydrogenase gene (adhE) in Escherichia coli by catabolite repressor activator protein Cra. J Bacteriol. 1997;179:7129-34 pubmed
    The adhE gene encodes ethanol dehydrogenase and is located at min 27.9 of the Escherichia coli chromosome...
  31. Rudolph F, Purich D, Fromm H. Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. I. Partial purification, properties, and kinetic studies of the enzyme. J Biol Chem. 1968;243:5539-45 pubmed
  32. Membrillo Hernandez J, Kwon O, De Wulf P, Finkel S, Lin E. Regulation of adhE (encoding ethanol oxidoreductase) by the Fis protein in Escherichia coli. J Bacteriol. 1999;181:7390-3 pubmed
    The adhE gene of Escherichia coli encodes a multifunctional ethanol oxidoreductase whose expression is 10-fold higher under anaerobic than aerobic conditions...
  33. Echave P, Esparza Cerón M, Cabiscol E, Tamarit J, Ros J, Membrillo Hernández J, et al. DnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia coli. Proc Natl Acad Sci U S A. 2002;99:4626-31 pubmed
    The adhE gene of Escherichia coli encodes a multifunctional ethanol oxidoreductase (AdhE) that catalyzes successive reductions of acetyl-CoA to acetaldehyde and then to ethanol reversibly at the expense of NADH...
  34. Clark D, Cronan J. Acetaldehyde coenzyme A dehydrogenase of Escherichia coli. J Bacteriol. 1980;144:179-84 pubmed
    ..The acd mutation was located at min 62 of the E. coli genetic map, the gene order being thyA-lysA-acd-serA-fda. Isolation of Tn10 insertions cotransducible with acd greatly simplified the mapping procedure. ..
  35. Leonardo M, Dailly Y, Clark D. Role of NAD in regulating the adhE gene of Escherichia coli. J Bacteriol. 1996;178:6013-8 pubmed
    The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels...
  36. Wyborn N, Messenger S, Henderson R, Sawers G, Roberts R, Attwood M, et al. Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products. Microbiology. 2002;148:1015-26 pubmed
    ..The YfiD radical was found to be introduced by the PFL-activase enzyme, but unlike PFL, AdhE did not deactivate radicalized YfiD...
  37. Pascal M, Chippaux M, Abou Jaoudé A, Blaschkowski H, Knappe J. Mutants of Escherichia coli K12 with defects in anaerobic pyruvate metabolism. J Gen Microbiol. 1981;124:35-42 pubmed
    A strain of Escherichia coli with a mutation in the ana gene was shown to lack acetaldehyde dehydrogenase and alcohol dehydrogenase...