ada

Summary

Gene Symbol: ada
Description: fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase
Alias: ECK2205, JW2201
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents
    P Jeggo
    J Bacteriol 139:783-91. 1979
  2. pmc Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription
    P Landini
    Swiss Federal Institute for Environmental Science and Technology EAWAG, 8600 Dubendorf, Switzerland
    J Bacteriol 181:6836-9. 1999
  3. pmc Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features
    P Landini
    Department of Environmental Microbiology and Molecular Ecotoxicology, Swiss Institute for Environmental Technology, 8600 Duebendorf, Switzerland
    J Bacteriol 182:6543-9. 2000
  4. ncbi Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions
    K Takano
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Mol Biol 201:261-71. 1988
  5. pmc Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents
    P K LeMotte
    J Bacteriol 161:888-95. 1985
  6. pmc Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator
    Y Nakabeppu
    Proc Natl Acad Sci U S A 83:6297-301. 1986
  7. ncbi Sequence-specific DNA recognition of the Escherichia coli Ada protein associated with the methylation-dependent functional switch for transcriptional regulation
    H Sakashita
    Protein Engineering Research Institute, Osaka
    J Biochem 118:1184-91. 1995
  8. ncbi DNA sequence analysis of spontaneous lacI-d mutations in O6-alkylguanine-DNA alkyltransferase-proficient and -deficient Escherichia coli
    A Vidal
    Departamento de Bioquimica, Universidad de Cordoba, Spain
    Mutagenesis 13:367-73. 1998
  9. ncbi Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity
    O Muzzin
    Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:15157-61. 1998
  10. ncbi Ada protein-RNA polymerase sigma subunit interaction and alpha subunit-promoter DNA interaction are necessary at different steps in transcription initiation at the Escherichia coli Ada and aidB promoters
    P Landini
    School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Biol Chem 273:13307-12. 1998

Scientific Experts

  • P Landini
  • Karol Kleibl
  • Jong Hwan Baek
  • Daniel T Haydon
  • Camilla Salmelin
  • Hiroto Takinowaki
  • J C Delaney
  • Yihan Lin
  • Peter T Rye
  • Jadwiga Nieminuszczy
  • Chuan He
  • Yuji Kobayashi
  • Tadayasu Ohkubo
  • Takuya Yoshida
  • Yasuhiro Matsuda
  • Jonathan J Wilker
  • G L Verdine
  • M Sekiguchi
  • Erica M Duguid
  • L C Myers
  • L J Sun
  • K Shiraki
  • Y Lin
  • J M Essigmann
  • P E Verdemato
  • Aaron R Dinner
  • Zishaan Farooqui
  • Xing Jian
  • P C Moody
  • Tong Zhao
  • Xiaohui Qu
  • Norbert F Scherer
  • James C Delaney
  • L M Posnick
  • B M Saget
  • Dana X Sun
  • Jenny Z Liu
  • John M Essigmann
  • Chawita Netirojjanakul
  • Elzbieta Grzesiuk
  • O Muzzin
  • H Sakashita
  • A Vidal
  • D Bhattacharyya
  • G Wagner
  • Y Nakabeppu
  • S R Paalman
  • Stephen J Lippard
  • M R Volkert
  • Yukiko Mishina
  • M H Moore
  • K Sakumi
  • L D Samson
  • A Taketomi
  • G C Walker
  • S Fujiwara
  • C K Yim
  • T Imanaka
  • M Takagi
  • T Wintner
  • H Hashimoto
  • S Nishikori
  • V Dotsch
  • Y Kai
  • K Peariso
  • J E Penner-Hahn
  • L Y Lian
  • F Zuccotto
  • C Damblon
  • J E Wibley
  • J A Brannigan
  • S Mitra
  • D E Shevell
  • T K Hazra
  • K Severinov
  • P L Chong
  • K Morikawa
  • M Kainosho
  • E A Campbell
  • L K Liem
  • W J Mackay
  • T Sakuma
  • S A Darst
  • B Sedgwick
  • T Ohkubo
  • J C Lee
  • L Xia
  • N Abril
  • A Kurosky
  • E Severinova

Detail Information

Publications76

  1. pmc Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents
    P Jeggo
    J Bacteriol 139:783-91. 1979
    ..These six strains have been called Ada mutants...
  2. pmc Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription
    P Landini
    Swiss Federal Institute for Environmental Science and Technology EAWAG, 8600 Dubendorf, Switzerland
    J Bacteriol 181:6836-9. 1999
    The Escherichia coli Ada protein activates sigma(70)-dependent transcription at three different promoters (ada, aidB, and alkA) in response to alkylation damage of DNA...
  3. pmc Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features
    P Landini
    Department of Environmental Microbiology and Molecular Ecotoxicology, Swiss Institute for Environmental Technology, 8600 Duebendorf, Switzerland
    J Bacteriol 182:6543-9. 2000
  4. ncbi Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions
    K Takano
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Mol Biol 201:261-71. 1988
    Specific cysteine residues at possible methyl acceptor sites of the Ada protein of Escherichia coli were converted to other amino acids by site-directed mutagenesis of the cloned ada gene of E. coli...
  5. pmc Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents
    P K LeMotte
    J Bacteriol 161:888-95. 1985
    The ada gene of Escherichia coli K-12, the regulatory locus for the adaptive response to methylating agents, coded for a 39,000-dalton protein. An adjacent gene coding for a 27,000-dalton protein was coregulated with ada...
  6. pmc Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator
    Y Nakabeppu
    Proc Natl Acad Sci U S A 83:6297-301. 1986
    Expression of the ada and alkA genes, both of which are involved in the adaptive response of Escherichia coli to alkylating agents, is positively controlled by Ada protein, the product of the ada gene...
  7. ncbi Sequence-specific DNA recognition of the Escherichia coli Ada protein associated with the methylation-dependent functional switch for transcriptional regulation
    H Sakashita
    Protein Engineering Research Institute, Osaka
    J Biochem 118:1184-91. 1995
    The Escherichia coli Ada protein, a suicidal DNA methyltransferase, is converted into a transcriptional regulator for methylation-resistance genes by the transfer of a methyl group from a DNA methylphosphotriester to its own Cys69 ..
  8. ncbi DNA sequence analysis of spontaneous lacI-d mutations in O6-alkylguanine-DNA alkyltransferase-proficient and -deficient Escherichia coli
    A Vidal
    Departamento de Bioquimica, Universidad de Cordoba, Spain
    Mutagenesis 13:367-73. 1998
    Spontaneous mutagenesis in O6-alkylguanine-DNA alkyltransferase-proficient and -deficient (ada ogt mutants) Escherichia coli was studied in two ways: in bacteria growing in nonselective liquid medium and in bacteria resting on selective ..
  9. ncbi Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity
    O Muzzin
    Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:15157-61. 1998
    ..An E. coli strain with a disruption in the hepA gene shows sensitivity to ultraviolet light...
  10. ncbi Ada protein-RNA polymerase sigma subunit interaction and alpha subunit-promoter DNA interaction are necessary at different steps in transcription initiation at the Escherichia coli Ada and aidB promoters
    P Landini
    School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Biol Chem 273:13307-12. 1998
    The methylated form of the Ada protein (meAda) binds the ada and aidB promoters between 60 and 40 base pairs upstream from the transcription start and activates transcription of the Escherichia coli ada and aidB genes...
  11. ncbi Reversible folding of Ada protein (O6-methylguanine-DNA methyltransferase) of Escherichia coli
    D Bhattacharyya
    University of Tennessee Graduate School of Biomedical Sciences, Biology Division, Oak Ridge National Laboratory, Tennessee 37831, USA
    Biochemistry 37:1722-30. 1998
    The multifunctional 39 kDa Escherichia coli Ada protein (O6-methylguanine-DNA methyltransferase) (EC 2.1.1...
  12. ncbi Specificity of DNA repair methyltransferases determined by competitive inactivation with oligonucleotide substrates: evidence that Escherichia coli Ada repairs O6-methylguanine and O4-methylthymine with similar efficiency
    S R Paalman
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Biochemistry 36:11118-24. 1997
    ..Contrary to previous reports, however, we find that Escherichia coli Ada repairs meG and meT with nearly equal efficiency...
  13. ncbi Context-dependent mutagenesis by DNA lesions
    J C Delaney
    Department of Chemistry, Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Chem Biol 6:743-53. 1999
    ..The respective contribution of these factors might be influenced by the DNA sequence context of the hotspot...
  14. ncbi Requirement for two conserved cysteine residues in the Ada protein of Escherichia coli for transactivation of the ada promoter
    A Taketomi
    Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    Mol Gen Genet 250:523-32. 1996
    Cysteine residue 69 of the Escherichia coli Ada transcription factor, which accepts a methyl group from methylphosphotriester in methylated DNA, was substituted by each of 19 other amino acids...
  15. ncbi Folding topology and DNA binding of the N-terminal fragment of Ada protein
    H Sakashita
    Protein Engineering Research Institute, Osaka, Japan
    FEBS Lett 323:252-6. 1993
    Three amino terminal fragments of Escherichia coli Ada protein (39 kDa) with different molecular masses (14 kDa, 16 kDa and 20 kDa) were prepared in large quantities from an E...
  16. pmc The Ada protein is a class I transcription factor of Escherichia coli
    K Sakumi
    Department of Biochemistry, Kyushu University, Fukuoka, Japan
    J Bacteriol 175:2455-7. 1993
    The methylated Ada protein of Escherichia coli, a regulatory protein for the adaptive response, binds to a target DNA from positions -62 to -31 upstream of the ada gene and facilitates the binding of RNA polymerase to the promoter...
  17. pmc DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli
    W J Mackay
    Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Bacteriol 176:3224-30. 1994
    The Escherichia coli Ada and Ogt DNA methyltransferases (MTases) are known to transfer simple alkyl groups from O6-alkylguanine and O4-alkylthymine, directly restoring these alkylated DNA lesions to guanine and thymine...
  18. pmc Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli
    M H Moore
    Department of Chemistry, University of York, UK
    EMBO J 13:1495-501. 1994
    ..Here we report the X-ray structure of the 19 kDa C-terminal domain of the Escherichia coli ada gene product, the prototype of these suicidal methyltransferases...
  19. pmc Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene
    M R Volkert
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 176:7638-45. 1994
    The Escherichia coli aidB gene is regulated by two different mechanisms, an ada-dependent pathway triggered by methyl damage to DNA and an ada-independent pathway triggered when cells are grown without aeration...
  20. pmc The Ada protein acts as both a positive and a negative modulator of Escherichia coli's response to methylating agents
    B M Saget
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139
    Proc Natl Acad Sci U S A 91:9730-4. 1994
    ..The major effector molecule regulating this response is the 39-kDa Ada protein, which functions as both a DNA repair protein and a transcriptional activator...
  21. pmc The Escherichia coli Ada protein can interact with two distinct determinants in the sigma70 subunit of RNA polymerase according to promoter architecture: identification of the target of Ada activation at the alkA promoter
    P Landini
    School of Biochemistry, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Bacteriol 181:1524-9. 1999
    The methylated form of the Ada protein (meAda) activates transcription from the Escherichia coli ada, aidB, and alkA promoters with different mechanisms...
  22. ncbi Bacterial DNA repair genes and their eukaryotic homologues: 3. AlkB dioxygenase and Ada methyltransferase in the direct repair of alkylated DNA
    Jadwiga Nieminuszczy
    Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Acta Biochim Pol 54:459-68. 2007
    ..Exposure of prokaryotic cells to sublethal doses of DNA alkylating agents induces so called adaptive response (Ada response) involving the expression of a set of genes which allows the cells to tolerate the toxic and mutagenic ..
  23. pmc Influence of S-adenosylmethionine pool size on spontaneous mutation, dam methylation, and cell growth of Escherichia coli
    L M Posnick
    Division of Toxicology, Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Bacteriol 181:6756-62. 1999
    Escherichia coli strains that are deficient in the Ada and Ogt DNA repair methyltransferases display an elevated spontaneous G:C-to-A:T transition mutation rate, and this increase has been attributed to mutagenic O(6)-alkylguanine ..
  24. pmc Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains
    Jong Hwan Baek
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology and Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
    BMC Microbiol 9:186. 2009
    The Ada-dependent adaptive response system in Escherichia coli is important for increasing resistance to alkylation damage. However, the global transcriptional and translational changes during this response have not been reported...
  25. pmc DNA-binding mechanism of the Escherichia coli Ada O(6)-alkylguanine-DNA alkyltransferase
    P E Verdemato
    Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK
    Nucleic Acids Res 28:3710-8. 2000
    The C-terminal domain of the Escherichia coli Ada protein (Ada-C) aids in the maintenance of genomic integrity by efficiently repairing pre-mutagenic O:(6)-alkylguanine lesions in DNA...
  26. ncbi Comparative analyses of the conformational stability of a hyperthermophilic protein and its mesophilic counterpart
    K Shiraki
    Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan
    Eur J Biochem 268:4144-50. 2001
    ..archaeon Thermococcus kodakaraensis strain KOD1 (Tk-MGMT), and its mesophilic counterpart C-terminal Ada protein from Escherichia coli (Ec-AdaC) was performed in order to obtain information about the relationship between ..
  27. ncbi Chemical communication across the zinc tetrathiolate cluster in Escherichia coli Ada, a metalloactivated DNA repair protein
    L J Sun
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Biochemistry 40:11596-603. 2001
    The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69...
  28. pmc expression profiling of all protein-coding genes in wild-type and three DNA repair-deficient substrains of Escherichia coli K-12
    Camilla Salmelin
    Leukemia Research Laboratory, Department of Clinical Chemistry, Tampere University Hospital, and Tampere University Medical School, Tampere, Finland
    Comp Funct Genomics 3:3-13. 2002
    ..genes in wild-type (WT) and three DNA repair-deficient derivative strains: (i) AB1157 (WT), (ii) LR39 (ada, ogt), (iii) MV1932 (alkA1, tag-1) and (iv) GM5555 (mutS)...
  29. ncbi Alkyl Transfer to Metal Thiolates: Kinetics, Active Species Identification, and Relevance to the DNA Methyl Phosphotriester Repair Center of Escherichia coli Ada
    Jonathan J Wilker
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    Inorg Chem 36:969-978. 1997
    The Ada protein of Escherichia coli employs a [Zn(S-cys)(4)](2)(-) site to repair deoxyribonucleic acid alkyl phosphotriester lesions. The alkyl group is transferred to a cysteine thiolate in a stoichiometric reaction...
  30. ncbi Effect of sequence context on O(6)-methylguanine repair and replication in vivo
    J C Delaney
    Department of Chemistry and Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 40:14968-75. 2001
    ..was used to address the contribution of sequence context on m(6)G repair by the Escherichia coli methyltransferases Ada or Ogt, and on DNA polymerase infidelity in vivo...
  31. pmc Mismatch repair proteins collaborate with methyltransferases in the repair of O(6)-methylguanine
    Peter T Rye
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    DNA Repair (Amst) 7:170-6. 2008
    ..Cysteine thiolate residues on the Ada and Ogt methyltransferase (MTase) proteins directly reverse the O(6)mG base damage to yield guanine...
  32. ncbi Molecular mechanisms of adaptive response to alkylating agents in Escherichia coli and some remarks on O(6)-methylguanine DNA-methyltransferase in other organisms
    Karol Kleibl
    Cancer Research Institute, Slovak Academy of Sciences, Vlarska 7, 833 91, Bratislava 37, Slovak Republic
    Mutat Res 512:67-84. 2002
    ..The MTase of E. coli, encoded by the ada gene was the first MTase to be discovered and one of best characterised...
  33. pmc Identifying reservoirs of infection: a conceptual and practical challenge
    Daniel T Haydon
    University of Edinburgh, Roslin, UK
    Emerg Infect Dis 8:1468-73. 2002
    ..We discuss the practical value of different approaches that may be used to identify reservoirs in the field...
  34. ncbi How do DNA repair proteins locate potential base lesions? a chemical crosslinking method to investigate O6-alkylguanine-DNA alkyltransferases
    Erica M Duguid
    Department of Chemistry, University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA
    Chem Biol 10:827-35. 2003
    ..to probe the damage-searching mechanism of two O(6)-alkylguanine-DNA alkyltransferases, the Escherichia coli C-Ada and the human AGT...
  35. ncbi 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein
    Hiroto Takinowaki
    J Biomol NMR 29:447-8. 2004
  36. ncbi Adaptive response of Escherichia coli to alkylation damage
    M R Volkert
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655
    Environ Mol Mutagen 11:241-55. 1988
    ..Four genes have been identified as components of this response, ada, alkA, alkB and aidB. The functions of two of these genes are known...
  37. ncbi Zinc binding by the methylation signaling domain of the Escherichia coli Ada protein
    L C Myers
    Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138
    Biochemistry 31:4541-7. 1992
    The Escherichia coli Ada protein repairs O6-methylguanine residues and methyl phosphotriesters in DNA by direct transfer of the methyl group to a cysteine residue located in its C- or N-terminal domain, respectively...
  38. pmc A region of the Ada DNA-repair protein required for the activation of ada transcription is not necessary for activation of alkA
    D E Shevell
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Proc Natl Acad Sci U S A 88:9001-5. 1991
    ..This response is controlled by the Ada protein, which not only functions as the transcriptional activator of the ada and alkA genes but also possesses two ..
  39. ncbi Roles of two types of O6-methylguanine-DNA methyltransferases in DNA repair
    K Takano
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Mutat Res 254:37-44. 1991
    ..These enzymes are coded by the ada and the ogt genes, respectively. Using a synthetic ogt-specific probe, we mapped ogt at 29...
  40. ncbi Positive control
    S Adhya
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 265:10797-800. 1990
  41. ncbi Expression of DNA damage-inducible genes of Escherichia coli upon treatment with methylating, ethylating and propylating agents
    M R Volkert
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655
    Mutat Res 217:109-15. 1989
    ..We find that the Ada-regulated adaptive response genes (ada-alkB, alkA and aidB) are induced primarily in response to methylation ..
  42. ncbi Regulation of expression of the ada gene controlling the adaptive response. Interactions with the ada promoter of the Ada protein and RNA polymerase
    K Sakumi
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Mol Biol 205:373-85. 1989
    The Ada protein of Escherichia coli catalyzes transfer of methyl groups from methylated DNA to its own molecule, and the methylated form of Ada protein promotes transcription of its own gene, ada...
  43. ncbi Activation of Ada protein as a transcriptional regulator by direct alkylation with methylating agents
    K Takahashi
    Faculty of Pharmaceutical Sciences, Nagoya City University, Japan
    J Biol Chem 263:13490-2. 1988
    ..In this process Ada protein, the product of the ada gene, plays a major role; it accepts the methyl groups of the methylated DNA at the ..
  44. ncbi Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase
    Y Nakabeppu
    J Biol Chem 260:7281-8. 1985
    The ada gene of Escherichia coli K12, the regulatory gene for the adaptive response of bacteria to alkylating agents, was cloned and placed under the control of the lac regulatory region on a multicopy runaway plasmid, thereby yielding a ..
  45. ncbi Proteolytic cleavage of Ada protein that carries methyltransferase and transcriptional regulator activities
    T Yoshikai
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Biol Chem 263:19174-80. 1988
    The 39-kDa Ada protein that carries two distinct methyltransferase activities and an activity to promote transcription of the ada and alkA genes is cleaved to smaller polypeptides in the case of incubation with a crude extract of ..
  46. ncbi Expression of the ada gene of Escherichia coli in response to alkylating agents. Identification of transcriptional regulatory elements
    T Nakamura
    Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Mol Biol 202:483-94. 1988
    b>Ada protein plays a central role in the regulatory synthesis of DNA repair enzymes, following exposure of Escherichia coli to alkylating agents...
  47. pmc Alteration of the carboxyl-terminal domain of Ada protein influences its inducibility, specificity, and strength as a transcriptional activator
    D E Shevell
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 170:5263-71. 1988
    The ada gene of Escherichia coli K-12 encodes the regulatory protein for the adaptive response to alkylating agents. A set of plasmids carrying ordered deletions from the 3' end of the ada gene were isolated and characterized...
  48. pmc Alteration of lysine 178 in the hinge region of the Escherichia coli ada protein interferes with activation of ada, but not alkA, transcription
    B M Saget
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 177:1268-74. 1995
    The ada gene of Escherichia coli K-12 encodes the 39-kDa Ada protein, which consists of two domains joined by a hinge region that is sensitive to proteolytic cleavage in vitro...
  49. ncbi Structure and expression of the alkB gene of Escherichia coli related to the repair of alkylated DNA
    H Kondo
    J Biol Chem 261:15772-7. 1986
    ..The nucleotide sequence analysis also revealed that the ada and alkB genes are adjacent on the E...
  50. ncbi Molecular cloning and characterization of the alkB gene of Escherichia coli
    H Kataoka
    Mol Gen Genet 198:263-9. 1985
    ..Introduction of a small deletion into the alkB region of the bacterial chromosome resulted in inactivation of both the alkB and ada genes, thereby suggesting that the two genes are adjacent on the E. coli chromosome.
  51. pmc Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis
    B Demple
    Proc Natl Acad Sci U S A 82:2688-92. 1985
    ..resistance to alkylation mutagenesis and killing in Escherichia coli (the adaptive response) is controlled by the ada gene...
  52. ncbi Transcriptional activation of the Escherichia coli adaptive response gene aidB is mediated by binding of methylated Ada protein. Evidence for a new consensus sequence for Ada-binding sites
    P Landini
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA
    J Biol Chem 270:8285-9. 1995
    ..Genes belonging to the adaptive response are positively regulated by the ada gene; the Ada protein acts as a transcriptional activator when methylated in one of its cysteine residues at ..
  53. ncbi A homology model of the three-dimensional structure of human O6-alkylguanine-DNA alkyltransferase based on the crystal structure of the C-terminal domain of the Ada protein from Escherichia coli
    J E Wibley
    Department of Pharmacy, University of Manchester, UK
    Anticancer Drug Des 10:75-95. 1995
    ..homology model of the human protein (hAT) was built, based on the crystal structure of the C-terminal domain of the Ada protein, which carries out a similar repair in Escherichia coli...
  54. pmc RNA polymerase alpha subunit binding site in positively controlled promoters: a new model for RNA polymerase-promoter interaction and transcriptional activation in the Escherichia coli ada and aidB genes
    P Landini
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA
    EMBO J 14:4329-35. 1995
    The ada and aidB genes are part of the adaptive response to DNA methylation damage in Escherichia coli...
  55. ncbi Adaptation to alkylation resistance involves the induction of a DNA glycosylase
    G Evensen
    Nature 296:773-5. 1982
  56. pmc Genetic mapping of ada and adc mutations affecting the adaptive response of Escherichia coli to alkylating agents
    B Sedgwick
    J Bacteriol 150:984-8. 1982
    ..Four mutations, ada-3, ada-5, ada-6, and adc-1, which confer differing phenotypes as regards this response, were shown to be ..
  57. ncbi Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response
    B Sedgwick
    Mol Gen Genet 180:85-90. 1980
    ..They have an even greater ability than the adapted wild type to remove O6MeG from methylated DNA (Lindahl personal communication)...
  58. pmc Cloning of the E. coli O6-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay
    G P Margison
    Nucleic Acids Res 13:1939-52. 1985
    ..These two activities reside on a 37Kd protein that can undergo a host-dependent cleavage to produce an 18Kd protein which contains only O6-meG MT and a 13Kd protein which contains only MP MT...
  59. pmc O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli
    S Mitra
    J Bacteriol 152:534-7. 1982
    ..Although both wild-type and adaptation-deficient (ada) mutants of E...
  60. ncbi Molecular cloning of a gene which regulates the adaptive response to alkylating agents in Escherichia coli
    B Sedgwick
    Mol Gen Genet 191:466-72. 1983
    The ada+ gene of E. coli is a regulatory gene of the adaptive response to simple alkylating agents...
  61. ncbi The intracellular signal for induction of resistance to alkylating agents in E. coli
    I Teo
    Cell 45:315-24. 1986
    The E. coli ada gene positively controls its own expression and that of other genes (alkA, alkB, aidB) involved in repair of DNA alkylation damage...
  62. ncbi Methyl Transfer to Mercury Thiolates: Effects of Coordination Number and Ligand Dissociation
    Jonathan J Wilker
    Departments of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and Dartmouth College, Hanover, New Hampshire 03755
    Inorg Chem 36:2079-2083. 1997
    ..These results imply that the metal center of the inactive mercury derivative of the Escherichia coli Ada DNA alkylation repair protein may comprise a three-coordinate [Hg(S-cysteine)(3)](-) moiety and an unbound, ..
  63. ncbi The RNA polymerase alpha subunit carboxyl-terminal domain is required for both basal and activated transcription from the alkA promoter
    P Landini
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 272:15914-9. 1997
    Expression of the Escherichia coli adaptive response genes (ada, aidB, and alkA) is regulated by the transcriptional activator, Ada. However, the interactions of RNA polymerase and Ada with these promoters differ...
  64. ncbi Structural basis for the functional switch of the E. coli Ada protein
    Y Lin
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Biochemistry 40:4261-71. 2001
    The Escherichia coli protein Ada specifically repairs the S(p) diastereomer of DNA methyl phosphotriesters in DNA by direct and irreversible transfer of the methyl group to its own Cys 69 which is part of a zinc-thiolate center...
  65. ncbi Induction of SOS response, cellular efflux and oxidative stress response genes by chlorambucil in DNA repair-deficient Escherichia coli cells (ada, ogt and mutS)
    Camilla Salmelin
    Department of Clinical Chemistry, Tampere University Hospital and Tampere University Medical School, PO Box 2000, FIN 33521 Tampere, Finland
    Mutat Res 522:33-44. 2003
    ..coli strains lacking either (i) two DNA methyltransferase functions (O(6)-methylguanine-DNA methyltransferase I (ada) and II (ogt)), or (ii) mismatch repair (MutS (mutS))...
  66. ncbi Metal-coordination sphere in the methylated Ada protein-DNA co-complex
    L C Myers
    Program for Higher Degrees in Biophysics, Harvard University, Cambridge, MA 02138, USA
    Chem Biol 1:91-7. 1994
    The Ada protein of Escherichia coli repairs methyl phosphotriesters in DNA by direct, irreversible methyl transfer to one of its own cysteine residues. This residue, Cys69, is ligated to a tightly bound zinc ion in the protein...
  67. pmc Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA
    L K Liem
    Chemical Carcinogenesis Laboratory, National University of Singapore, Kent Ridge
    Nucleic Acids Res 22:1613-9. 1994
    ..coli 39kD Ada protein (km6G/ke6G = 1.6). However, the human MGMT is very different (km6G/ke6G = 163)...
  68. pmc The solution structure of the methylated form of the N-terminal 16-kDa domain of Escherichia coli Ada protein
    Hiroto Takinowaki
    Graduate School of Pharmaceutical Sciences, Osaka University, 1 6 Yamadaoka, Suita, Osaka 565 0871, Japan
    Protein Sci 15:487-97. 2006
    The N-terminal 16-kDa domain of Escherichia coli Ada protein (N-Ada16k) repairs DNA methyl phosphotriester lesions by an irreversible methyl transfer to its cysteine residue...
  69. pmc Induction of specific Escherichia coli genes by sublethal treatments with alkylating agents
    M R Volkert
    Proc Natl Acad Sci U S A 81:4110-4. 1984
    ..The ada-5 mutation, which blocks expression of the adaptive response, decreased induction of beta-galactosidase activity in ..
  70. ncbi The suicidal DNA repair methyltransferases of microbes
    L Samson
    Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, Massachusetts 02115
    Mol Microbiol 6:825-31. 1992
    ..This review considers the structure, function and biological roles of a number of well-characterized microbial DNA repair MTases...
  71. pmc Using the bias from flow to elucidate single DNA repair protein sliding and interactions with DNA
    Yihan Lin
    Department of Chemistry, The University of Chicago, Chicago, Illinois 60637, USA
    Biophys J 96:1911-7. 2009
    We perform single-molecule spatial tracking measurements of a DNA repair protein, the C-terminal domain of Ada (C-Ada) from Escherichia coli, moving on DNA extended by flow...