ada

Summary

Gene Symbol: ada
Description: fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase
Alias: ECK2205, JW2201
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Jeggo P. Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents. J Bacteriol. 1979;139:783-91 pubmed
    ..These six strains have been called Ada mutants...
  2. Landini P, Busby S. Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription. J Bacteriol. 1999;181:6836-9 pubmed
    The Escherichia coli Ada protein activates sigma(70)-dependent transcription at three different promoters (ada, aidB, and alkA) in response to alkylation damage of DNA...
  3. Landini P, Volkert M. Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features. J Bacteriol. 2000;182:6543-9 pubmed
  4. LeMotte P, Walker G. Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents. J Bacteriol. 1985;161:888-95 pubmed
    The ada gene of Escherichia coli K-12, the regulatory locus for the adaptive response to methylating agents, coded for a 39,000-dalton protein. An adjacent gene coding for a 27,000-dalton protein was coregulated with ada...
  5. Takano K, Nakabeppu Y, Sekiguchi M. Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions. J Mol Biol. 1988;201:261-71 pubmed
    Specific cysteine residues at possible methyl acceptor sites of the Ada protein of Escherichia coli were converted to other amino acids by site-directed mutagenesis of the cloned ada gene of E. coli...
  6. Nakabeppu Y, Sekiguchi M. Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator. Proc Natl Acad Sci U S A. 1986;83:6297-301 pubmed
    Expression of the ada and alkA genes, both of which are involved in the adaptive response of Escherichia coli to alkylating agents, is positively controlled by Ada protein, the product of the ada gene...
  7. Sedgwick B, Lindahl T. Recent progress on the Ada response for inducible repair of DNA alkylation damage. Oncogene. 2002;21:8886-94 pubmed
  8. Landini P, Volkert M. Transcriptional activation of the Escherichia coli adaptive response gene aidB is mediated by binding of methylated Ada protein. Evidence for a new consensus sequence for Ada-binding sites. J Biol Chem. 1995;270:8285-9 pubmed
    ..Genes belonging to the adaptive response are positively regulated by the ada gene; the Ada protein acts as a transcriptional activator when methylated in one of its cysteine residues at ..
  9. Teo I, Sedgwick B, Kilpatrick M, McCarthy T, Lindahl T. The intracellular signal for induction of resistance to alkylating agents in E. coli. Cell. 1986;45:315-24 pubmed
    The E. coli ada gene positively controls its own expression and that of other genes (alkA, alkB, aidB) involved in repair of DNA alkylation damage...
  10. Landini P, Busby S. The Escherichia coli Ada protein can interact with two distinct determinants in the sigma70 subunit of RNA polymerase according to promoter architecture: identification of the target of Ada activation at the alkA promoter. J Bacteriol. 1999;181:1524-9 pubmed
    The methylated form of the Ada protein (meAda) activates transcription from the Escherichia coli ada, aidB, and alkA promoters with different mechanisms...

Detail Information

Publications71

  1. Jeggo P. Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents. J Bacteriol. 1979;139:783-91 pubmed
    ..These six strains have been called Ada mutants...
  2. Landini P, Busby S. Expression of the Escherichia coli ada regulon in stationary phase: evidence for rpoS-dependent negative regulation of alkA transcription. J Bacteriol. 1999;181:6836-9 pubmed
    The Escherichia coli Ada protein activates sigma(70)-dependent transcription at three different promoters (ada, aidB, and alkA) in response to alkylation damage of DNA...
  3. Landini P, Volkert M. Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features. J Bacteriol. 2000;182:6543-9 pubmed
  4. LeMotte P, Walker G. Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents. J Bacteriol. 1985;161:888-95 pubmed
    The ada gene of Escherichia coli K-12, the regulatory locus for the adaptive response to methylating agents, coded for a 39,000-dalton protein. An adjacent gene coding for a 27,000-dalton protein was coregulated with ada...
  5. Takano K, Nakabeppu Y, Sekiguchi M. Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions. J Mol Biol. 1988;201:261-71 pubmed
    Specific cysteine residues at possible methyl acceptor sites of the Ada protein of Escherichia coli were converted to other amino acids by site-directed mutagenesis of the cloned ada gene of E. coli...
  6. Nakabeppu Y, Sekiguchi M. Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator. Proc Natl Acad Sci U S A. 1986;83:6297-301 pubmed
    Expression of the ada and alkA genes, both of which are involved in the adaptive response of Escherichia coli to alkylating agents, is positively controlled by Ada protein, the product of the ada gene...
  7. Sedgwick B, Lindahl T. Recent progress on the Ada response for inducible repair of DNA alkylation damage. Oncogene. 2002;21:8886-94 pubmed
  8. Landini P, Volkert M. Transcriptional activation of the Escherichia coli adaptive response gene aidB is mediated by binding of methylated Ada protein. Evidence for a new consensus sequence for Ada-binding sites. J Biol Chem. 1995;270:8285-9 pubmed
    ..Genes belonging to the adaptive response are positively regulated by the ada gene; the Ada protein acts as a transcriptional activator when methylated in one of its cysteine residues at ..
  9. Teo I, Sedgwick B, Kilpatrick M, McCarthy T, Lindahl T. The intracellular signal for induction of resistance to alkylating agents in E. coli. Cell. 1986;45:315-24 pubmed
    The E. coli ada gene positively controls its own expression and that of other genes (alkA, alkB, aidB) involved in repair of DNA alkylation damage...
  10. Landini P, Busby S. The Escherichia coli Ada protein can interact with two distinct determinants in the sigma70 subunit of RNA polymerase according to promoter architecture: identification of the target of Ada activation at the alkA promoter. J Bacteriol. 1999;181:1524-9 pubmed
    The methylated form of the Ada protein (meAda) activates transcription from the Escherichia coli ada, aidB, and alkA promoters with different mechanisms...
  11. Sun L, Yim C, Verdine G. Chemical communication across the zinc tetrathiolate cluster in Escherichia coli Ada, a metalloactivated DNA repair protein. Biochemistry. 2001;40:11596-603 pubmed
    The Escherichia coli Ada protein repairs methylphosphotriesters in DNA through direct, irreversible transfer to a cysteine residue on the protein, Cys 69...
  12. Lin Y, Zhao T, Jian X, Farooqui Z, Qu X, He C, et al. Using the bias from flow to elucidate single DNA repair protein sliding and interactions with DNA. Biophys J. 2009;96:1911-7 pubmed publisher
    We perform single-molecule spatial tracking measurements of a DNA repair protein, the C-terminal domain of Ada (C-Ada) from Escherichia coli, moving on DNA extended by flow...
  13. Volkert M, Nguyen D. Induction of specific Escherichia coli genes by sublethal treatments with alkylating agents. Proc Natl Acad Sci U S A. 1984;81:4110-4 pubmed
    ..The ada-5 mutation, which blocks expression of the adaptive response, decreased induction of beta-galactosidase activity in ..
  14. Takano K, Nakamura T, Sekiguchi M. Roles of two types of O6-methylguanine-DNA methyltransferases in DNA repair. Mutat Res. 1991;254:37-44 pubmed
    ..These enzymes are coded by the ada and the ogt genes, respectively. Using a synthetic ogt-specific probe, we mapped ogt at 29...
  15. Haydon D, Cleaveland S, Taylor L, Laurenson M. Identifying reservoirs of infection: a conceptual and practical challenge. Emerg Infect Dis. 2002;8:1468-73 pubmed
    ..We discuss the practical value of different approaches that may be used to identify reservoirs in the field. ..
  16. Landini P, Bown J, Volkert M, Busby S. Ada protein-RNA polymerase sigma subunit interaction and alpha subunit-promoter DNA interaction are necessary at different steps in transcription initiation at the Escherichia coli Ada and aidB promoters. J Biol Chem. 1998;273:13307-12 pubmed
    The methylated form of the Ada protein (meAda) binds the ada and aidB promoters between 60 and 40 base pairs upstream from the transcription start and activates transcription of the Escherichia coli ada and aidB genes...
  17. Myers L, Terranova M, Nash H, Markus M, Verdine G. Zinc binding by the methylation signaling domain of the Escherichia coli Ada protein. Biochemistry. 1992;31:4541-7 pubmed
    The Escherichia coli Ada protein repairs O6-methylguanine residues and methyl phosphotriesters in DNA by direct transfer of the methyl group to a cysteine residue located in its C- or N-terminal domain, respectively...
  18. Wibley J, McKie J, Embrey K, Marks D, Douglas K, Moore M, et al. A homology model of the three-dimensional structure of human O6-alkylguanine-DNA alkyltransferase based on the crystal structure of the C-terminal domain of the Ada protein from Escherichia coli. Anticancer Drug Des. 1995;10:75-95 pubmed
    ..homology model of the human protein (hAT) was built, based on the crystal structure of the C-terminal domain of the Ada protein, which carries out a similar repair in Escherichia coli...
  19. Demple B, Sedgwick B, Robins P, Totty N, Waterfield M, Lindahl T. Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis. Proc Natl Acad Sci U S A. 1985;82:2688-92 pubmed
    ..resistance to alkylation mutagenesis and killing in Escherichia coli (the adaptive response) is controlled by the ada gene...
  20. Sakumi K, Sekiguchi M. Regulation of expression of the ada gene controlling the adaptive response. Interactions with the ada promoter of the Ada protein and RNA polymerase. J Mol Biol. 1989;205:373-85 pubmed
    The Ada protein of Escherichia coli catalyzes transfer of methyl groups from methylated DNA to its own molecule, and the methylated form of Ada protein promotes transcription of its own gene, ada...
  21. Lin Y, Dotsch V, Wintner T, Peariso K, Myers L, Penner Hahn J, et al. Structural basis for the functional switch of the E. coli Ada protein. Biochemistry. 2001;40:4261-71 pubmed
    The Escherichia coli protein Ada specifically repairs the S(p) diastereomer of DNA methyl phosphotriesters in DNA by direct and irreversible transfer of the methyl group to its own Cys 69 which is part of a zinc-thiolate center...
  22. Myers L, Cushing T, Wagner G, Verdine G. Metal-coordination sphere in the methylated Ada protein-DNA co-complex. Chem Biol. 1994;1:91-7 pubmed
    The Ada protein of Escherichia coli repairs methyl phosphotriesters in DNA by direct, irreversible methyl transfer to one of its own cysteine residues. This residue, Cys69, is ligated to a tightly bound zinc ion in the protein...
  23. Vidal A, Abril N, Pueyo C. DNA sequence analysis of spontaneous lacI-d mutations in O6-alkylguanine-DNA alkyltransferase-proficient and -deficient Escherichia coli. Mutagenesis. 1998;13:367-73 pubmed
    Spontaneous mutagenesis in O6-alkylguanine-DNA alkyltransferase-proficient and -deficient (ada ogt mutants) Escherichia coli was studied in two ways: in bacteria growing in nonselective liquid medium and in bacteria resting on selective ..
  24. Saget B, Walker G. The Ada protein acts as both a positive and a negative modulator of Escherichia coli's response to methylating agents. Proc Natl Acad Sci U S A. 1994;91:9730-4 pubmed
    ..The major effector molecule regulating this response is the 39-kDa Ada protein, which functions as both a DNA repair protein and a transcriptional activator...
  25. Shevell D, LeMotte P, Walker G. Alteration of the carboxyl-terminal domain of Ada protein influences its inducibility, specificity, and strength as a transcriptional activator. J Bacteriol. 1988;170:5263-71 pubmed
    The ada gene of Escherichia coli K-12 encodes the regulatory protein for the adaptive response to alkylating agents. A set of plasmids carrying ordered deletions from the 3' end of the ada gene were isolated and characterized...
  26. Wilker J, Lippard S. Alkyl Transfer to Metal Thiolates: Kinetics, Active Species Identification, and Relevance to the DNA Methyl Phosphotriester Repair Center of Escherichia coli Ada. Inorg Chem. 1997;36:969-978 pubmed
    The Ada protein of Escherichia coli employs a [Zn(S-cys)(4)](2)(-) site to repair deoxyribonucleic acid alkyl phosphotriester lesions. The alkyl group is transferred to a cysteine thiolate in a stoichiometric reaction...
  27. Evensen G, Seeberg E. Adaptation to alkylation resistance involves the induction of a DNA glycosylase. Nature. 1982;296:773-5 pubmed
  28. Landini P, Volkert M. RNA polymerase alpha subunit binding site in positively controlled promoters: a new model for RNA polymerase-promoter interaction and transcriptional activation in the Escherichia coli ada and aidB genes. EMBO J. 1995;14:4329-35 pubmed
    The ada and aidB genes are part of the adaptive response to DNA methylation damage in Escherichia coli...
  29. Volkert M. Adaptive response of Escherichia coli to alkylation damage. Environ Mol Mutagen. 1988;11:241-55 pubmed
    ..Four genes have been identified as components of this response, ada, alkA, alkB and aidB. The functions of two of these genes are known...
  30. Salmelin C, Vilpo J. Induction of SOS response, cellular efflux and oxidative stress response genes by chlorambucil in DNA repair-deficient Escherichia coli cells (ada, ogt and mutS). Mutat Res. 2003;522:33-44 pubmed
    ..coli strains lacking either (i) two DNA methyltransferase functions (O(6)-methylguanine-DNA methyltransferase I (ada) and II (ogt)), or (ii) mismatch repair (MutS (mutS))...
  31. Sedgwick B, Robins P. Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response. Mol Gen Genet. 1980;180:85-90 pubmed
    ..They have an even greater ability than the adapted wild type to remove O6MeG from methylated DNA (Lindahl personal communication). ..
  32. Landini P, Gaal T, Ross W, Volkert M. The RNA polymerase alpha subunit carboxyl-terminal domain is required for both basal and activated transcription from the alkA promoter. J Biol Chem. 1997;272:15914-9 pubmed
    Expression of the Escherichia coli adaptive response genes (ada, aidB, and alkA) is regulated by the transcriptional activator, Ada. However, the interactions of RNA polymerase and Ada with these promoters differ...
  33. Verdemato P, Brannigan J, Damblon C, Zuccotto F, Moody P, Lian L. DNA-binding mechanism of the Escherichia coli Ada O(6)-alkylguanine-DNA alkyltransferase. Nucleic Acids Res. 2000;28:3710-8 pubmed
    The C-terminal domain of the Escherichia coli Ada protein (Ada-C) aids in the maintenance of genomic integrity by efficiently repairing pre-mutagenic O:(6)-alkylguanine lesions in DNA...
  34. Delaney J, Essigmann J. Effect of sequence context on O(6)-methylguanine repair and replication in vivo. Biochemistry. 2001;40:14968-75 pubmed
    ..was used to address the contribution of sequence context on m(6)G repair by the Escherichia coli methyltransferases Ada or Ogt, and on DNA polymerase infidelity in vivo...
  35. Moore M, Gulbis J, Dodson E, Demple B, Moody P. Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli. EMBO J. 1994;13:1495-501 pubmed
    ..Here we report the X-ray structure of the 19 kDa C-terminal domain of the Escherichia coli ada gene product, the prototype of these suicidal methyltransferases...
  36. Posnick L, Samson L. Influence of S-adenosylmethionine pool size on spontaneous mutation, dam methylation, and cell growth of Escherichia coli. J Bacteriol. 1999;181:6756-62 pubmed
    Escherichia coli strains that are deficient in the Ada and Ogt DNA repair methyltransferases display an elevated spontaneous G:C-to-A:T transition mutation rate, and this increase has been attributed to mutagenic O(6)-alkylguanine ..
  37. Nieminuszczy J, Grzesiuk E. Bacterial DNA repair genes and their eukaryotic homologues: 3. AlkB dioxygenase and Ada methyltransferase in the direct repair of alkylated DNA. Acta Biochim Pol. 2007;54:459-68 pubmed
    ..Exposure of prokaryotic cells to sublethal doses of DNA alkylating agents induces so called adaptive response (Ada response) involving the expression of a set of genes which allows the cells to tolerate the toxic and mutagenic ..
  38. Sakashita H, Sakuma T, Ohkubo T, Kainosho M, Sakumi K, Sekiguchi M, et al. Folding topology and DNA binding of the N-terminal fragment of Ada protein. FEBS Lett. 1993;323:252-6 pubmed
    Three amino terminal fragments of Escherichia coli Ada protein (39 kDa) with different molecular masses (14 kDa, 16 kDa and 20 kDa) were prepared in large quantities from an E...
  39. Mackay W, Han S, Samson L. DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli. J Bacteriol. 1994;176:3224-30 pubmed
    The Escherichia coli Ada and Ogt DNA methyltransferases (MTases) are known to transfer simple alkyl groups from O6-alkylguanine and O4-alkylthymine, directly restoring these alkylated DNA lesions to guanine and thymine...
  40. Takahashi K, Kawazoe Y, Sakumi K, Nakabeppu Y, Sekiguchi M. Activation of Ada protein as a transcriptional regulator by direct alkylation with methylating agents. J Biol Chem. 1988;263:13490-2 pubmed
    ..In this process Ada protein, the product of the ada gene, plays a major role; it accepts the methyl groups of the methylated DNA at the ..
  41. Sedgwick B. Molecular cloning of a gene which regulates the adaptive response to alkylating agents in Escherichia coli. Mol Gen Genet. 1983;191:466-72 pubmed
    The ada+ gene of E. coli is a regulatory gene of the adaptive response to simple alkylating agents...
  42. Sakashita H, Sakuma T, Akitomo Y, Ohkubo T, Kainosho M, Sekiguchi M, et al. Sequence-specific DNA recognition of the Escherichia coli Ada protein associated with the methylation-dependent functional switch for transcriptional regulation. J Biochem. 1995;118:1184-91 pubmed
    The Escherichia coli Ada protein, a suicidal DNA methyltransferase, is converted into a transcriptional regulator for methylation-resistance genes by the transfer of a methyl group from a DNA methylphosphotriester to its own Cys69 ..
  43. Delaney J, Essigmann J. Context-dependent mutagenesis by DNA lesions. Chem Biol. 1999;6:743-53 pubmed
    ..In partially repair-proficient cells, the Ada O6-methylguanine-DNA methyltransferase repair protein was twice as efficient on m6G when a guanine base rather than ..
  44. Kataoka H, Sekiguchi M. Molecular cloning and characterization of the alkB gene of Escherichia coli. Mol Gen Genet. 1985;198:263-9 pubmed
    ..Introduction of a small deletion into the alkB region of the bacterial chromosome resulted in inactivation of both the alkB and ada genes, thereby suggesting that the two genes are adjacent on the E. coli chromosome.
  45. Mitra S, Pal B, Foote R. O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli. J Bacteriol. 1982;152:534-7 pubmed
    ..Although both wild-type and adaptation-deficient (ada) mutants of E...
  46. Shevell D, Walker G. A region of the Ada DNA-repair protein required for the activation of ada transcription is not necessary for activation of alkA. Proc Natl Acad Sci U S A. 1991;88:9001-5 pubmed
    ..This response is controlled by the Ada protein, which not only functions as the transcriptional activator of the ada and alkA genes but also possesses two ..
  47. Paalman S, Sung C, Clarke N. Specificity of DNA repair methyltransferases determined by competitive inactivation with oligonucleotide substrates: evidence that Escherichia coli Ada repairs O6-methylguanine and O4-methylthymine with similar efficiency. Biochemistry. 1997;36:11118-24 pubmed
    ..Contrary to previous reports, however, we find that Escherichia coli Ada repairs meG and meT with nearly equal efficiency...
  48. Salmelin C, Vilpo J. expression profiling of all protein-coding genes in wild-type and three DNA repair-deficient substrains of Escherichia coli K-12. Comp Funct Genomics. 2002;3:3-13 pubmed publisher
    ..genes in wild-type (WT) and three DNA repair-deficient derivative strains: (i) AB1157 (WT), (ii) LR39 (ada, ogt), (iii) MV1932 (alkA1, tag-1) and (iv) GM5555 (mutS)...
  49. Volkert M, Gately F, Hajec L. Expression of DNA damage-inducible genes of Escherichia coli upon treatment with methylating, ethylating and propylating agents. Mutat Res. 1989;217:109-15 pubmed
    ..We find that the Ada-regulated adaptive response genes (ada-alkB, alkA and aidB) are induced primarily in response to methylation ..
  50. Kondo H, Nakabeppu Y, Kataoka H, Kuhara S, Kawabata S, Sekiguchi M. Structure and expression of the alkB gene of Escherichia coli related to the repair of alkylated DNA. J Biol Chem. 1986;261:15772-7 pubmed
    ..The nucleotide sequence analysis also revealed that the ada and alkB genes are adjacent on the E...
  51. Margison G, Cooper D, Brennand J. Cloning of the E. coli O6-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay. Nucleic Acids Res. 1985;13:1939-52 pubmed
    ..These two activities reside on a 37Kd protein that can undergo a host-dependent cleavage to produce an 18Kd protein which contains only O6-meG MT and a 13Kd protein which contains only MP MT. ..
  52. Baek J, Han M, Lee S, Yoo J. Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains. BMC Microbiol. 2009;9:186 pubmed publisher
    The Ada-dependent adaptive response system in Escherichia coli is important for increasing resistance to alkylation damage. However, the global transcriptional and translational changes during this response have not been reported...
  53. Nakabeppu Y, Kondo H, Kawabata S, Iwanaga S, Sekiguchi M. Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase. J Biol Chem. 1985;260:7281-8 pubmed
    The ada gene of Escherichia coli K12, the regulatory gene for the adaptive response of bacteria to alkylating agents, was cloned and placed under the control of the lac regulatory region on a multicopy runaway plasmid, thereby yielding a ..
  54. Teo I, Sedgwick B, Demple B, Li B, Lindahl T. Induction of resistance to alkylating agents in E. coli: the ada+ gene product serves both as a regulatory protein and as an enzyme for repair of mutagenic damage. EMBO J. 1984;3:2151-7 pubmed
    The expression of several inducible enzymes for repair of alkylated DNA in Escherichia coli is controlled by the ada+ gene. This regulatory gene has been cloned into a multicopy plasmid and shown to code for a 37-kd protein...
  55. Lindahl T, Demple B, Robins P. Suicide inactivation of the E. coli O6-methylguanine-DNA methyltransferase. EMBO J. 1982;1:1359-63 pubmed
    ..1% of the rate for duplex DNA. This latter observation may explain the high frequency of mutations induced by alkylating agents at DNA replication forks. ..
  56. Volkert M, Hajec L, Matijasevic Z, Fang F, Prince R. Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene. J Bacteriol. 1994;176:7638-45 pubmed
    The Escherichia coli aidB gene is regulated by two different mechanisms, an ada-dependent pathway triggered by methyl damage to DNA and an ada-independent pathway triggered when cells are grown without aeration...
  57. Muzzin O, Campbell E, Xia L, Severinova E, Darst S, Severinov K. Disruption of Escherichia coli hepA, an RNA polymerase-associated protein, causes UV sensitivity. J Biol Chem. 1998;273:15157-61 pubmed
    ..An E. coli strain with a disruption in the hepA gene shows sensitivity to ultraviolet light. ..
  58. Liem L, Lim A, Li B. Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA. Nucleic Acids Res. 1994;22:1613-9 pubmed
    ..coli 39kD Ada protein (km6G/ke6G = 1.6). However, the human MGMT is very different (km6G/ke6G = 163)...
  59. Taketomi A, Nakabeppu Y, Ihara K, Hart D, Furuichi M, Sekiguchi M. Requirement for two conserved cysteine residues in the Ada protein of Escherichia coli for transactivation of the ada promoter. Mol Gen Genet. 1996;250:523-32 pubmed
    Cysteine residue 69 of the Escherichia coli Ada transcription factor, which accepts a methyl group from methylphosphotriester in methylated DNA, was substituted by each of 19 other amino acids...
  60. Nakamura T, Tokumoto Y, Sakumi K, Koike G, Nakabeppu Y, Sekiguchi M. Expression of the ada gene of Escherichia coli in response to alkylating agents. Identification of transcriptional regulatory elements. J Mol Biol. 1988;202:483-94 pubmed
    b>Ada protein plays a central role in the regulatory synthesis of DNA repair enzymes, following exposure of Escherichia coli to alkylating agents...
  61. Kleibl K. Molecular mechanisms of adaptive response to alkylating agents in Escherichia coli and some remarks on O(6)-methylguanine DNA-methyltransferase in other organisms. Mutat Res. 2002;512:67-84 pubmed
    ..The MTase of E. coli, encoded by the ada gene was the first MTase to be discovered and one of best characterised...
  62. Schendel P, Robins P. Repair of O6-methylguanine in adapted Escherichia coli. Proc Natl Acad Sci U S A. 1978;75:6017-20 pubmed
    ..Our data support the idea that the O6 methylation of guanine accounts for most MNNG-induced mutagenesis. ..
  63. Takinowaki H, Matsuda Y, Yoshida T, Kobayashi Y, Ohkubo T. 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein. J Biomol NMR. 2004;29:447-8 pubmed
  64. Adhya S, Garges S. Positive control. J Biol Chem. 1990;265:10797-800 pubmed
  65. Rye P, Delaney J, Netirojjanakul C, Sun D, Liu J, Essigmann J. Mismatch repair proteins collaborate with methyltransferases in the repair of O(6)-methylguanine. DNA Repair (Amst). 2008;7:170-6 pubmed
    ..Cysteine thiolate residues on the Ada and Ogt methyltransferase (MTase) proteins directly reverse the O(6)mG base damage to yield guanine...
  66. Bhattacharyya D, Hazra T, Behnke W, Chong P, Kurosky A, Lee J, et al. Reversible folding of Ada protein (O6-methylguanine-DNA methyltransferase) of Escherichia coli. Biochemistry. 1998;37:1722-30 pubmed
    The multifunctional 39 kDa Escherichia coli Ada protein (O6-methylguanine-DNA methyltransferase) (EC 2.1.1...
  67. Shiraki K, Nishikori S, Fujiwara S, Hashimoto H, Kai Y, Takagi M, et al. Comparative analyses of the conformational stability of a hyperthermophilic protein and its mesophilic counterpart. Eur J Biochem. 2001;268:4144-50 pubmed
    ..archaeon Thermococcus kodakaraensis strain KOD1 (Tk-MGMT), and its mesophilic counterpart C-terminal Ada protein from Escherichia coli (Ec-AdaC) was performed in order to obtain information about the relationship between ..
  68. Saget B, Shevell D, Walker G. Alteration of lysine 178 in the hinge region of the Escherichia coli ada protein interferes with activation of ada, but not alkA, transcription. J Bacteriol. 1995;177:1268-74 pubmed
    The ada gene of Escherichia coli K-12 encodes the 39-kDa Ada protein, which consists of two domains joined by a hinge region that is sensitive to proteolytic cleavage in vitro...
  69. Yoshikai T, Nakabeppu Y, Sekiguchi M. Proteolytic cleavage of Ada protein that carries methyltransferase and transcriptional regulator activities. J Biol Chem. 1988;263:19174-80 pubmed
    The 39-kDa Ada protein that carries two distinct methyltransferase activities and an activity to promote transcription of the ada and alkA genes is cleaved to smaller polypeptides in the case of incubation with a crude extract of ..