Gene Symbol: acnB
Description: aconitate hydratase 2; aconitase B; 2-methyl-cis-aconitate hydratase
Alias: ECK0117, JW0114, yacI, yacJ
Species: Escherichia coli str. K-12 substr. MG1655
Products:     acnB

Top Publications

  1. Rahman M, Shimizu K. Altered acetate metabolism and biomass production in several Escherichia coli mutants lacking rpoS-dependent metabolic pathway genes. Mol Biosyst. 2008;4:160-9 pubmed publisher
    ..On the other hand, the fumC and acnA mutants, with their impaired TCA cycles, were unable to utilize acetate for biomass production in spite of the higher expression of rpoS and soxRS. ..
  2. Cunningham L, Gruer M, Guest J. Transcriptional regulation of the aconitase genes (acnA and acnB) of Escherichia coli. Microbiology. 1997;143 ( Pt 12):3795-805 pubmed
    Escherichia coli contains two differentially regulated aconitase genes, acnA and acnB. Two acnA promoters transcribing from start points located 407 bp (P1acnA) and 50 bp (P2acnA) upstream of the acnA coding region, and one acnB promoter (..
  3. Tang Y, Guest J. Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitases. Microbiology. 1999;145 ( Pt 11):3069-79 pubmed
    ..aconitase (AcnA) that is induced by iron and oxidative stress, and a major but less stable aconitase (AcnB) synthesized during exponential growth...
  4. Tang Y, Quail M, Artymiuk P, Guest J, Green J. Escherichia coli aconitases and oxidative stress: post-transcriptional regulation of sodA expression. Microbiology. 2002;148:1027-37 pubmed
    ..stationary-phase enzyme (AcnA), which is induced by iron and oxidative stress, and a major but less stable enzyme (AcnB), synthesized during exponential growth...
  5. Varghese S, Tang Y, Imlay J. Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletion. J Bacteriol. 2003;185:221-30 pubmed
    ..More recently it was determined that E. coli synthesizes at least two isozymes of aconitase, AcnA and AcnB. Synthesis of AcnA, the less-abundant enzyme, is positively controlled by SoxS, a protein that is activated in the ..
  6. Kiley P, Beinert H. The role of Fe-S proteins in sensing and regulation in bacteria. Curr Opin Microbiol. 2003;6:181-5 pubmed
    ..Therefore, these regulatory factors provide insights into the chemical versatility of Fe-S clusters. ..
  7. Gruer M, Artymiuk P, Guest J. The aconitase family: three structural variations on a common theme. Trends Biochem Sci. 1997;22:3-6 pubmed
  8. Wright G. On the road to bacterial cell death. Cell. 2007;130:781-3 pubmed
    ..In this issue, Kohanski et al. (2007) provide evidence that the production of reactive oxygen species is a shared mechanism of cell death initiated by bactericidal antibiotics. ..
  9. Justino M, Almeida C, Teixeira M, Saraiva L. Escherichia coli di-iron YtfE protein is necessary for the repair of stress-damaged iron-sulfur clusters. J Biol Chem. 2007;282:10352-9 pubmed
    ..We concluded that YtfE is critical for the repair of iron-sulfur clusters damaged by oxidative and nitrosative stress conditions. ..

More Information


  1. Karow M, Raina S, Georgopoulos C, Fayet O. Complex phenotypes of null mutations in the htr genes, whose products are essential for Escherichia coli growth at elevated temperatures. Res Microbiol. 1991;142:289-94 pubmed
    ..The htrM gene is identical to the rfaD gene, whose product is required for the biosynthesis of the lipopolysaccharide precursor ADP-L-glycero-D-mannoheptose (Pegues et al., J. Bact., 1990, 172, 4652-4660). ..
  2. Jordan P, Tang Y, Bradbury A, Thomson A, Guest J. Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB). Biochem J. 1999;344 Pt 3:739-46 pubmed
    Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains...
  3. Williams C, Stillman T, Barynin V, Sedelnikova S, Tang Y, Green J, et al. E. coli aconitase B structure reveals a HEAT-like domain with implications for protein-protein recognition. Nat Struct Biol. 2002;9:447-52 pubmed
    The major bifunctional aconitase of Escherichia coli (AcnB) serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster...
  4. Bradbury A, Gruer M, Rudd K, Guest J. The second aconitase (AcnB) of Escherichia coli. Microbiology. 1996;142 ( Pt 2):389-400 pubmed
    The second aconitase (AcnB) of Escherichia coli was partially purified from an acnA::kanR mutant lacking AcnA, and the corresponding polypeptide identified by activity staining and weak cross-reactivity with AcnA antiserum...
  5. Tsuchiya D, Shimizu N, Tomita M. Versatile architecture of a bacterial aconitase B and its catalytic performance in the sequential reaction coupled with isocitrate dehydrogenase. Biochim Biophys Acta. 2008;1784:1847-56 pubmed publisher
    Aconitase B (AcnB) and isocitrate dehydrogenase (ICDH) catalyze the sequential reaction in the Krebs cycle...
  6. Benjamin J, Massé E. The iron-sensing aconitase B binds its own mRNA to prevent sRNA-induced mRNA cleavage. Nucleic Acids Res. 2014;42:10023-36 pubmed publisher
    ..In Escherichia coli, aconitase B (AcnB) is a typical moonlighting protein that can switch to its apo form (apo-AcnB) which favors binding its own mRNA 3'..
  7. Calderón I, Elías A, Fuentes E, Pradenas G, Castro M, Arenas F, et al. Tellurite-mediated disabling of [4Fe-4S] clusters of Escherichia coli dehydratases. Microbiology. 2009;155:1840-6 pubmed publisher
    ..Reactive oxygen species (ROS)-sensitive fumarase A (FumA) and aconitase B (AcnB) as well as ROS-resistant fumarase C (FumC) and aconitase A (AcnA) were assayed in cell-free extracts from ..
  8. Brock M, Maerker C, Schütz A, Volker U, Buckel W. Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase. Eur J Biochem. 2002;269:6184-94 pubmed
    ..requires a novel enzyme, methylcitrate dehydratase (PrpD), and the well-known enzyme, aconitase (AcnB), of the tricarboxylic acid cycle. AcnB was purified as 2-methylaconitate hydratase from E...
  9. Tang Y, Guest J, Artymiuk P, Green J. Switching aconitase B between catalytic and regulatory modes involves iron-dependent dimer formation. Mol Microbiol. 2005;56:1149-58 pubmed
    In addition to being the major citric acid cycle aconitase in Escherichia coli the aconitase B protein (AcnB) is also a post-transcriptional regulator of gene expression...
  10. Tsuchiya D, Shimizu N, Tomita M. Cooperativity of two active sites in bacterial homodimeric aconitases. Biochem Biophys Res Commun. 2009;379:485-8 pubmed publisher
    ..Escherichia coli possesses two kinds of aconitases, aconitase A (AcnA) and B (AcnB), whose structural organizations are different...
  11. Tan G, Lu J, Bitoun J, Huang H, Ding H. IscA/SufA paralogues are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions. Biochem J. 2009;420:463-72 pubmed publisher
    ..coli under aerobic conditions. ..
  12. Fischer E, Sauer U. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. J Biol Chem. 2003;278:46446-51 pubmed
  13. Gruer M, Guest J. Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli. Microbiology. 1994;140 ( Pt 10):2531-41 pubmed
  14. Kohanski M, Dwyer D, Hayete B, Lawrence C, Collins J. A common mechanism of cellular death induced by bactericidal antibiotics. Cell. 2007;130:797-810 pubmed
    ..g., reca. ..
  15. Gardner P, Fridovich I. Effect of glutathione on aconitase in Escherichia coli. Arch Biochem Biophys. 1993;301:98-102 pubmed
    ..coli. GSH may facilitate reactivation of aconitase, and of other [4Fe-4S]-containing dehydratases, by increasing the rate of transfer of Fe(II) to the [3Fe-4S] site. ..
  16. Pomposiello P, Koutsolioutsou A, Carrasco D, Demple B. SoxRS-regulated expression and genetic analysis of the yggX gene of Escherichia coli. J Bacteriol. 2003;185:6624-32 pubmed
    ..These results demonstrate the direct regulation of the yggX gene by the redox-sensing SoxRS system and provide further evidence for the involvement of yggX in protection of iron-sulfur proteins against oxidative damage. ..
  17. Gruer M, Bradbury A, Guest J. Construction and properties of aconitase mutants of Escherichia coli. Microbiology. 1997;143 ( Pt 6):1837-46 pubmed
    Escherichia coli contains two genes (acnA and acnB) encoding aconitase activities. An acnB mutant was engineered by replacing the chromosomal acnB gene by an internally deleted derivative containing a tetR cassette...