Gene Symbol: aceA
Description: isocitrate lyase
Alias: ECK4007, JW3975, icl
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Phue J, Noronha S, Hattacharyya R, Wolfe A, Shiloach J. Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analyses. Biotechnol Bioeng. 2005;90:805-20 pubmed
    ..The gluconeogenesis pathway (fbp, pckA, and ppsA) which leads to glycogen accumulation is constitutively active in E. coli BL21 regardless of glucose feeding strategy. ..
  2. Chung T, Klumpp D, LaPorte D. Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map. J Bacteriol. 1988;170:386-92 pubmed
    ..The metabolic enzymes, isocitrate lyase and malate synthase, are expressed from aceA and aceB, and the regulatory enzyme, isocitrate dehydrogenase kinase/phosphatase, is expressed from aceK...
  3. Klumpp D, Plank D, Bowdin L, Stueland C, Chung T, LaPorte D. Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase. J Bacteriol. 1988;170:2763-9 pubmed
    ..Multiple copies of the REP family of repetitive extragenic elements were found within the intergenic region between aceA (encoding isocitrate lyase) and aceK...
  4. Byrne C, Stokes H, Ward K. Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli. Nucleic Acids Res. 1988;16:10924 pubmed
  5. Abeysinghe S, Baker P, Rice D, Rodgers H, Stillman T, Ko Y, et al. Use of chemical modification in the crystallization of isocitrate lyase from Escherichia coli. J Mol Biol. 1991;220:13-6 pubmed
    Two different crystal forms of isocitrate lyase (ICL) from Escherichia coli have been grown following the chemical modification of the enzyme by either 3-bromopyruvate or ethyl mercuri thiosalicylate (EMTS), contrasting strongly with ..
  6. Cortay J, Bleicher F, Duclos B, Cenatiempo Y, Gautier C, Prato J, et al. Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operon. Biochimie. 1989;71:1043-9 pubmed presented, including the 3 structural genes coding respectively for malate synthase (aceB), isocitrate lyase (aceA) and isocitrate dehydrogenase kinase/phosphatase (aceK), and the surrounding genes iclR and metA...
  7. Matsuoka M, McFadden B. Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli. J Bacteriol. 1988;170:4528-36 pubmed
    ..7-kilobase-pair DNA segment was sufficient for complementation of an aceA deletion mutation and overproduction of isocitrate lyase...
  8. Fischer E, Sauer U. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. J Biol Chem. 2003;278:46446-51 pubmed
  9. Britton K, Abeysinghe I, Baker P, Barynin V, Diehl P, Langridge S, et al. The structure and domain organization of Escherichia coli isocitrate lyase. Acta Crystallogr D Biol Crystallogr. 2001;57:1209-18 pubmed

More Information


  1. Gill R, Valdes J, Bentley W. A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metab Eng. 2000;2:178-89 pubmed
    ..the responses included significantly increased upregulation of heat shock (ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and bacteriophage life cycle (..
  2. Diehl P, McFadden B. The importance of four histidine residues in isocitrate lyase from Escherichia coli. J Bacteriol. 1994;176:927-31 pubmed
    ..All the H-197, H-266, and H-306 mutants supported the growth of isocitrate lyase-deficient E. coli JE10 on acetate as the sole carbon source; however, the H-184 mutants did not. ..
  3. Gawand P, Said Abukar F, Venayak N, Partow S, Motter A, Mahadevan R. Sub-optimal phenotypes of double-knockout mutants of Escherichia coli depend on the order of gene deletions. Integr Biol (Camb). 2015;7:930-9 pubmed publisher
    ..Specifically, we investigated the effects of the deletion of the glyoxylate-shunt gene aceA (encoding a latent reaction enzyme, isocitrate lyase) on the growth characteristics of the mutant E. coli Δpgi...
  4. Ogawa T, Murakami K, Mori H, Ishii N, Tomita M, Yoshin M. Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli. J Bacteriol. 2007;189:1176-8 pubmed
  5. Prasad Maharjan R, Yu P, Seeto S, Ferenci T. The role of isocitrate lyase and the glyoxylate cycle in Escherichia coli growing under glucose limitation. Res Microbiol. 2005;156:178-83 pubmed
    ..The role of isocitrate lyase (AceA) was examined in E. coli growing under glucose limitation...
  6. Blankenhorn D, Phillips J, Slonczewski J. Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J Bacteriol. 1999;181:2209-16 pubmed
    ..Isocitrate lyase (AceA) was induced by oxygen across the pH range but showed substantially greater induction in acid or in base than at pH ..
  7. Hoyt J, Robertson E, Berlyn K, Reeves H. Escherichia coli isocitrate lyase: properties and comparisons. Biochim Biophys Acta. 1988;966:30-5 pubmed
    ..coli isocitrate lyase and compared these results to those obtained with other organisms. ..
  8. Maloy S, Nunn W. Genetic regulation of the glyoxylate shunt in Escherichia coli K-12. J Bacteriol. 1982;149:173-80 pubmed
    ..The genes for the two unique enzymes of the glyoxylate shunt, aceA and aceB, are located at 90 min on the E. coli K-12 genetic map...
  9. Rieul C, Bleicher F, Duclos B, Cortay J, Cozzone A. Nucleotide sequence of the aceA gene coding for isocitrate lyase in Escherichia coli. Nucleic Acids Res. 1988;16:5689 pubmed
  10. Wick L, Quadroni M, Egli T. Short- and long-term changes in proteome composition and kinetic properties in a culture of Escherichia coli during transition from glucose-excess to glucose-limited growth conditions in continuous culture and vice versa. Environ Microbiol. 2001;3:588-99 pubmed upregulation of 12 proteins, namely MglB, MalE, ArgT, DppA, RbsB, YdcS, LivJ (precursor), UgpB (precursor), AceA, AldA, AtpA and GatY. Eight of these proteins are periplasmic binding proteins of ABC transporters...
  11. Yamamoto K, Ishihama A. Two different modes of transcription repression of the Escherichia coli acetate operon by IclR. Mol Microbiol. 2003;47:183-94 pubmed
    IclR is a repressor for the Escherichia coli aceBAK operon, which encodes isocitrate lyase (aceB), malate synthase (aceA) and isocitrate dehydroge-nase kinase/phosphorylase (aceK) in the glyoxylate bypass...
  12. Robertson A, Nimmo H. Site-directed mutagenesis of cysteine-195 in isocitrate lyase from Escherichia coli ML308. Biochem J. 1995;305 ( Pt 1):239-44 pubmed
    Cysteine-195 was previously identified as a probable active site residue in isocitrate lyase (ICL) from Escherichia coli ML308 [Nimmo, Douglas, Kleanthous, Campbell and MacKintosh (1989) Biochem. J. 261, 431-435]...
  13. Chung T, Resnik E, Stueland C, LaPorte D. Relative expression of the products of glyoxylate bypass operon: contributions of transcription and translation. J Bacteriol. 1993;175:4572-5 pubmed
    ..The upshift in expression between aceB and aceA resulted from differences in translational efficiency...
  14. Mackintosh C, Nimmo H. Purification and regulatory properties of isocitrate lyase from Escherichia coli ML308. Biochem J. 1988;250:25-31 pubmed
    ..coli cells growing on acetate, about one order of magnitude higher than the Km determined in vitro in the absence of competing anions. ..
  15. Robertson E, Hoyt J, Reeves H. Evidence of histidine phosphorylation in isocitrate lyase from Escherichia coli. J Biol Chem. 1988;263:2477-82 pubmed
    ..In addition, loss of catalytic activity after treatment with potato acid phosphatase indicates that catalytically active isocitrate lyase is the phosphorylated form of the enzyme. ..
  16. Ko Y, Vanni P, McFadden B. The interaction of 3-phosphoglycerate and other substrate analogs with the glyoxylate- and succinate-binding sites of isocitrate lyase. Arch Biochem Biophys. 1989;274:155-60 pubmed
    ..The interaction of other substrate analogs, including glycolate, oxalate, phosphoenolpyruvate, and cis-aconitate, with isocitrate lyase from E. coli is also characterized. ..
  17. Gui L, Sunnarborg A, LaPorte D. Regulated expression of a repressor protein: FadR activates iclR. J Bacteriol. 1996;178:4704-9 pubmed
    ..We observed a similar increase when we inactivated the FadR binding site of an iclR+ allele. This result suggests that FadR regulates aceBAK indirectly by altering the expression of IclR. ..
  18. el Mansi E, Mackintosh C, Duncan K, Holms W, Nimmo H. Molecular cloning and over-expression of the glyoxylate bypass operon from Escherichia coli ML308. Biochem J. 1987;242:661-5 pubmed
    ..This plasmid complements an aceA mutation...
  19. Nimmo H, Douglas F, Kleanthous C, Campbell D, Mackintosh C. Identification of a cysteine residue at the active site of Escherichia coli isocitrate lyase. Biochem J. 1989;261:431-5 pubmed
    ..The reactivity of isocitrate lyase to iodoacetate declined with pH, following a titration curve for a group of pKa 7.1. The Km of the enzyme for isocritrate declined over the same pH range...
  20. Peskov K, Goryanin I, Prank K, Tobin F, Demin O. Kinetic modeling of ace operon genetic regulation in Escherichia coli. J Bioinform Comput Biol. 2008;6:933-59 pubmed
    ..coli B strain carries two coeffector binding sites. Using model-developed levels of steady state aceBAK expression against physiological ranges of coeffectors, concentration has been predicted. ..
  21. Creaghan I, Guest J. Succinate dehydrogenase-dependent nutritional requirement for succinate in mutants of Escherichia coli K12. J Gen Microbiol. 1978;107:1-13 pubmed
    ..of mutations affecting 2-oxoglutarate dehydrogenase (sucA), succinate dehydrogenase (sdh), isocitrate lyase (aceA) and fumarate reductase (frd) in a background lacking succinate semialdehyde dehydrogenase, was constructed to ..