ECP_2439

Summary

Gene Symbol: ECP_2439
Description: phosphohistidinoprotein-hexose phosphotransferase component of PTS system (Hpr)
Species:

Top Publications

  1. ncbi Active-centre torsion-angle strain revealed in 1.6 A-resolution structure of histidine-containing phosphocarrier protein
    Z Jia
    Department of Chemistry, University of Saskatchewan, Saskatoon, Canada
    Nature 361:94-7. 1993
  2. ncbi Mutation of serine-46 to aspartate in the histidine-containing protein of Escherichia coli mimics the inactivation by phosphorylation of serine-46 in HPrs from gram-positive bacteria
    S Napper
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    Biochemistry 35:11260-7. 1996
  3. ncbi Solution structure of the 30 kDa N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system by multidimensional NMR
    D S Garrett
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA
    Biochemistry 36:2517-30. 1997
  4. ncbi Tautomeric state and pKa of the phosphorylated active site histidine in the N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system
    D S Garrett
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Protein Sci 7:789-93. 1998
  5. ncbi The 2.5 A resolution structure of the jel42 Fab fragment/HPr complex
    L Prasad
    Health Science Building, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, S7N 5E5, Canada
    J Mol Biol 280:829-45. 1998
  6. ncbi Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr
    D S Garrett
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    Nat Struct Biol 6:166-73. 1999
  7. ncbi The aspartyl replacement of the active site histidine in histidine-containing protein, HPr, of the Escherichia coli Phosphoenolpyruvate:Sugar phosphotransferase system can accept and donate a phosphoryl group. Spontaneous dephosphorylation of acyl-phospha
    S Napper
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    J Biol Chem 274:21776-82. 1999
  8. ncbi Solution structure of the phosphoryl transfer complex between the signal transducing proteins HPr and IIA(glucose) of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system
    G Wang
    Laboratory of Chemical Physics, Building 5, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0510, USA
    EMBO J 19:5635-49. 2000
  9. ncbi Solution structure of the phosphoryl transfer complex between the cytoplasmic A domain of the mannitol transporter IIMannitol and HPr of the Escherichia coli phosphotransferase system
    Gabriel Cornilescu
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:42289-98. 2002
  10. ncbi Positive regulation of the expression of the Escherichia coli pts operon. Identification of the regulatory regions
    H De Reuse
    Unité de Régulation de l Expression Génétique, Institut Pasteur, Paris, France
    J Mol Biol 226:623-35. 1992

Detail Information

Publications21

  1. ncbi Active-centre torsion-angle strain revealed in 1.6 A-resolution structure of histidine-containing phosphocarrier protein
    Z Jia
    Department of Chemistry, University of Saskatchewan, Saskatoon, Canada
    Nature 361:94-7. 1993
    ..An HPr phosphotransfer cycling mechanism is proposed, based primarily on the structures of HPr and other phosphotransferase system proteins...
  2. ncbi Mutation of serine-46 to aspartate in the histidine-containing protein of Escherichia coli mimics the inactivation by phosphorylation of serine-46 in HPrs from gram-positive bacteria
    S Napper
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    Biochemistry 35:11260-7. 1996
    ..The lack of substantial change at the active center of E. coli HPr Ser46Asp HPr suggests that the effect of the Ser46 phosphorylation in HPrs from Gram-positive bacteria is due to an electrostatic interference with enzyme I binding...
  3. ncbi Solution structure of the 30 kDa N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system by multidimensional NMR
    D S Garrett
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA
    Biochemistry 36:2517-30. 1997
    ..Since His189 is thought to be phosphorylated at the N epsilon2 position, its side chain conformation would have to change upon phosphorylation...
  4. ncbi Tautomeric state and pKa of the phosphorylated active site histidine in the N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system
    D S Garrett
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Protein Sci 7:789-93. 1998
    ....
  5. ncbi The 2.5 A resolution structure of the jel42 Fab fragment/HPr complex
    L Prasad
    Health Science Building, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, S7N 5E5, Canada
    J Mol Biol 280:829-45. 1998
    ..Although a specific amino acid at residue 12 is not conserved in HPr molecules from all species, a hydrogen bond between the side-chains of residue 12 and HisP15 may be a conserved feature of the active centres...
  6. ncbi Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr
    D S Garrett
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0520, USA
    Nat Struct Biol 6:166-73. 1999
    ..The transition state can be formed with minimal changes in overall conformation, and is stabilized in favor of phosphorylated HPr, thereby accounting for the directionality of phosphoryl transfer...
  7. ncbi The aspartyl replacement of the active site histidine in histidine-containing protein, HPr, of the Escherichia coli Phosphoenolpyruvate:Sugar phosphotransferase system can accept and donate a phosphoryl group. Spontaneous dephosphorylation of acyl-phospha
    S Napper
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    J Biol Chem 274:21776-82. 1999
    ....
  8. ncbi Solution structure of the phosphoryl transfer complex between the signal transducing proteins HPr and IIA(glucose) of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system
    G Wang
    Laboratory of Chemical Physics, Building 5, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0510, USA
    EMBO J 19:5635-49. 2000
    ....
  9. ncbi Solution structure of the phosphoryl transfer complex between the cytoplasmic A domain of the mannitol transporter IIMannitol and HPr of the Escherichia coli phosphotransferase system
    Gabriel Cornilescu
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:42289-98. 2002
    ....
  10. ncbi Positive regulation of the expression of the Escherichia coli pts operon. Identification of the regulatory regions
    H De Reuse
    Unité de Régulation de l Expression Génétique, Institut Pasteur, Paris, France
    J Mol Biol 226:623-35. 1992
    ..We finally propose that the expression of the pts operon is controlled by two alternative positive regulatory mechanisms, which are designed to allow activation of the pts operon under a great variety of growth conditions...
  11. ncbi The 2.0-A resolution structure of Escherichia coli histidine-containing phosphocarrier protein HPr. A redetermination
    Z Jia
    Department of Chemistry, University of Saskatchewan, Saskatoon, Canada
    J Biol Chem 268:22490-501. 1993
    ..The semi-closed conformation observed in the E. coli HPr structure could represent a structural intermediate on the phosphorylation/dephosphorylation pathway of HPr...
  12. ncbi The high-resolution structure of the histidine-containing phosphocarrier protein HPr from Escherichia coli determined by restrained molecular dynamics from nuclear magnetic resonance nuclear Overhauser effect data
    N A Van Nuland
    Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands
    J Mol Biol 237:544-59. 1994
    ..This suggests that the strain may be an artifact of crystallization conditions instead of an essential element in the phosphorylation/dephosphorylation process...
  13. ncbi Determination of the three-dimensional solution structure of the histidine-containing phosphocarrier protein HPr from Escherichia coli using multidimensional NMR spectroscopy
    N A Van Nuland
    BIOSON Research Institute, University of Groningen, The Netherlands
    Eur J Biochem 210:881-91. 1992
    ..Reddy, P., Sutrina, S., Saier, M. H., Reizer, J. & Kapafia, G. (1992) Proc. Natl, Acad. Sci. USA 89, 2499-2503)...
  14. ncbi Epitope mapping by mutagenesis distinguishes between the two tertiary structures of the histidine-containing protein HPr
    S Sharma
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    Proc Natl Acad Sci U S A 88:4877-81. 1991
    ..Thus the x-ray structure may represent a partially unfolded HPr...
  15. ncbi Effect of phosphorylation on hydrogen-bonding interactions of the active site histidine of the phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system determined by 15N NMR spectroscopy
    A A van Dijk
    Department of Physical Chemistry, State University of Groningen, The Netherlands
    Biochemistry 29:8164-71. 1990
    ..The hydrogen bond to the His15 N delta 1 breaks upon phosphorylation of the residue. Implications of the H-bond structure for the mechanism of phosphorylation of HPr are discussed...
  16. ncbi Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: nucleotide sequence of the ptsH gene
    H De Reuse
    Gene 35:199-207. 1985
    ..Analysis of the nucleotide sequence substantiates the notion that the ptsH-ptsI-crr genes constitute a polycistronic operon...
  17. ncbi Crystallization of the complex of a monoclonal Fab fragment with the histidine-containing protein of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli
    L T Delbaere
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    J Biol Chem 264:18645-6. 1989
    ..The space group is C2, with unit cell dimensions a = 130.0, b = 68.1, and c = 77.6 A; beta = 97.3 degrees; and Z = 4. There is one Fab-histidine-containing protein complex/asymmetric unit, and the solvent content is estimated to be 57%...
  18. ncbi Structure determination of a monoclonal Fab fragment specific for histidine-containing protein of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli
    L Prasad
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    J Biol Chem 263:2571-4. 1988
    ..There is no local noncrystallographic axis of symmetry relating the two molecules in the asymmetric unit...
  19. ncbi Evaluation of mutagenesis for epitope mapping. Structure of an antibody-protein antigen complex
    L Prasad
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada
    J Biol Chem 268:10705-8. 1993
    ..Guidelines are also presented in order to minimize incorrect assignments...
  20. ncbi High-resolution structure of the phosphorylated form of the histidine-containing phosphocarrier protein HPr from Escherichia coli determined by restrained molecular dynamics from NMR-NOE data
    N A Van Nuland
    Groningen Biomolecular Sciences and Biotechnology Institute, The Netherlands
    J Mol Biol 246:180-93. 1995
    ....
  21. ncbi Solution NMR structure of the 48-kDa IIAMannose-HPr complex of the Escherichia coli mannose phosphotransferase system
    David C Williams
    Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:20775-84. 2005
    ....