Gene Symbol: TJP1
Description: tight junction protein 1
Alias: ZO-1, ZO1, tight junction protein ZO-1, tight junction protein 1 (zona occludens 1), zonula occludens protein 1
Species: dog
Products:     TJP1

Top Publications

  1. Van Itallie C, Fanning A, Bridges A, Anderson J. ZO-1 stabilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton. Mol Biol Cell. 2009;20:3930-40 pubmed publisher
    ..These studies are the first direct evidence that ZO-1 limits solute permeability in established tight junctions, perhaps by forming a stabilizing link between the barrier and perijunctional actomyosin. ..
  2. Pulimeno P, Paschoud S, Citi S. A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells. J Biol Chem. 2011;286:16743-50 pubmed publisher
    ..In summary, we characterize ZO-1 and PLEKHA7 as paracingulin-interacting proteins that are involved in its recruitment to epithelial tight and adherens junctions, respectively. ..
  3. Fanning A, Van Itallie C, Anderson J. Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia. Mol Biol Cell. 2012;23:577-90 pubmed publisher
  4. McNeil E, Capaldo C, Macara I. Zonula occludens-1 function in the assembly of tight junctions in Madin-Darby canine kidney epithelial cells. Mol Biol Cell. 2006;17:1922-32 pubmed
    ..These data reveal an unexpected function for the SH3 domain of ZO-1 in regulating tight junction assembly in epithelial cells and show that cingulin, occludin, or ZO-2 are not limiting for junction assembly in MDCK monolayers. ..
  5. Dunn C, Lampe P. Injury-triggered Akt phosphorylation of Cx43: a ZO-1-driven molecular switch that regulates gap junction size. J Cell Sci. 2014;127:455-64 pubmed publisher
  6. Maiers J, Peng X, Fanning A, DeMali K. ZO-1 recruitment to ?-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions. J Cell Sci. 2013;126:3904-15 pubmed publisher
    ..Thus, our study identifies ?-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion...
  7. Ooshio T, Kobayashi R, Ikeda W, Miyata M, Fukumoto Y, Matsuzawa N, et al. Involvement of the interaction of afadin with ZO-1 in the formation of tight junctions in Madin-Darby canine kidney cells. J Biol Chem. 2010;285:5003-12 pubmed publisher
    ..These results indicate that the transient interaction of afadin with ZO-1 is necessary for the formation of TJs in MDCK cells...
  8. Rajasekaran A, Hojo M, Huima T, Rodriguez Boulan E. Catenins and zonula occludens-1 form a complex during early stages in the assembly of tight junctions. J Cell Biol. 1996;132:451-63 pubmed
  9. Kawajiri A, Itoh N, Fukata M, Nakagawa M, Yamaga M, Iwamatsu A, et al. Identification of a novel beta-catenin-interacting protein. Biochem Biophys Res Commun. 2000;273:712-7 pubmed
    ..KIAA0313 and beta-catenin were partly colocalized at sites of cell-cell contact in MDCKII cells. Taken together, our data suggest that KIAA0313 associates with beta-catenin through KIAA0705 in vivo at sites of cell-cell contact. ..

More Information


  1. Tokuda S, Higashi T, Furuse M. ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape. PLoS ONE. 2014;9:e104994 pubmed publisher
    ..These results suggest a role for ZO-1 in the regulation of cytoskeleton and shape of cell-cell junctions in MDCK cells and indicate the advantage of knockout analysis in cultured cells. ..
  2. Tsapara A, Matter K, Balda M. The heat-shock protein Apg-2 binds to the tight junction protein ZO-1 and regulates transcriptional activity of ZONAB. Mol Biol Cell. 2006;17:1322-30 pubmed
  3. Elias B, Suzuki T, Seth A, Giorgianni F, Kale G, Shen L, et al. Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions. J Biol Chem. 2009;284:1559-69 pubmed publisher
    ..This study reveals a unique motif in the occludin sequence that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues. ..
  4. Ahn C, Shin D, Lee D, Kang S, Seok J, Kang H, et al. Expression of claudins, occludin, junction adhesion molecule A and zona occludens 1 in canine organs. Mol Med Rep. 2016;14:3697-703 pubmed publisher
    ..These results suggest that tight junctions are differently expressed in organs, and therefore may be involved in organ?specific functions to maintain physiological homeostasis. ..
  5. Nagaoka K, Udagawa T, Richter J. CPEB-mediated ZO-1 mRNA localization is required for epithelial tight-junction assembly and cell polarity. Nat Commun. 2012;3:675 pubmed publisher
    ..Our data demonstrate that CPEB-mediated zonal occludens-1 mRNA localization is essential for tight-junction assembly and mammary epithelial cell polarity...
  6. Lee N, Tong M, Leung P, Chan V, Leung S, Tam P, et al. Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease. FEBS Lett. 2006;580:923-31 pubmed
    ..Taken together, claudin-19 is a claudin isoform that is highly and specifically expressed in renal tubules with a putative role in TJ homeostasis in renal physiology. ..
  7. Lehtonen S, Lehtonen E, Kudlicka K, Holthofer H, Farquhar M. Nephrin forms a complex with adherens junction proteins and CASK in podocytes and in Madin-Darby canine kidney cells expressing nephrin. Am J Pathol. 2004;165:923-36 pubmed
  8. Sjö A, Magnusson K, Peterson K. Association of alpha-dystrobrevin with reorganizing tight junctions. J Membr Biol. 2005;203:21-30 pubmed
    ..Moreover, actin and ZO-1 co-immunoprecipitated with a-DB, as displayed with immunoblotting. Our findings suggest that a-dystrobrevin specifically is associated with the tight junctions during their reorganization. ..
  9. Roussel A, Bruet V, Marsella R, Knol A, Bourdeau P. Tight junction proteins in the canine epidermis: a pilot study on their distribution in normal and in high IgE-producing canines. Can J Vet Res. 2015;79:46-51 pubmed
    ..Ces résultats suggèrent un défaut d’expression de la Claudine-1 dans l’épiderme non lésionnel des chiens atopiques.(Traduit par les auteurs). ..
  10. Unda F, Perez Nanclares G, Le Morvan V, Hernandez C, Vilaxa A, de la Fuente M, et al. Dynamic assembly of tight junction-associated proteins ZO-1, ZO-2, ZO-3 and occludin during mouse tooth development. Histol Histopathol. 2003;18:27-38 pubmed publisher
    ..The occurrence and relation during tooth development of tight junction proteins ZO-1, ZO-2 and occludin, but not ZO-3, suggests a combinatory assembly in tooth morphogenesis and cell differentiation. ..
  11. Gonzalez Mariscal L, Islas S, Contreras R, García Villegas M, Betanzos A, Vega J, et al. Molecular characterization of the tight junction protein ZO-1 in MDCK cells. Exp Cell Res. 1999;248:97-109 pubmed
    ..16 kb long. MDCK-ZO-1 mRNA is 7.4 kb long. Its expression is regulated by calcium, while the expression of MDCK-ZO-1 protein is not. ..