dmsB

Summary

Gene Symbol: dmsB
Description: dimethyl sulfoxide reductase, anaerobic, subunit B
Alias: ECK0886, JW0878
Species:

Top Publications

  1. ncbi Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli
    P A Cotter
    Department of Microbiology, University of California, Los Angeles
    J Bacteriol 171:3817-23. 1989
  2. ncbi Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli
    P T Bilous
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Mol Microbiol 2:785-95. 1988
  3. ncbi Purification and properties of Escherichia coli dimethyl sulfoxide reductase, an iron-sulfur molybdoenzyme with broad substrate specificity
    J H Weiner
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Bacteriol 170:1505-10. 1988
  4. ncbi H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells
    A V Bogachev
    Department of Bioenergetics, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    J Bacteriol 178:6233-7. 1996
  5. ncbi Oxygen and nitrate-dependent regulation of dmsABC operon expression in Escherichia coli: sites for Fnr and NarL protein interactions
    Shawn M D Bearson
    Department of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, University of California, Los Angeles, CA 90095, USA
    BMC Microbiol 2:13. 2002
  6. ncbi Alteration of the iron-sulfur cluster composition of Escherichia coli dimethyl sulfoxide reductase by site-directed mutagenesis
    R A Rothery
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biochemistry 30:8296-305. 1991
  7. ncbi The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC)
    Shannon P Lubitz
    CIHR Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Alta, T6G 2H7, Edmonton, Canada
    Arch Biochem Biophys 418:205-16. 2003
  8. ncbi Common principles in the biosynthesis of diverse enzymes
    R L Jack
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England, UK
    Biochem Soc Trans 33:105-7. 2005
  9. ncbi Investigation of the environment surrounding iron-sulfur cluster 4 of Escherichia coli dimethylsulfoxide reductase
    Victor W T Cheng
    Membrane Protein Research Group, Department of Biochemistry, University of Alberta, 474 Medical Sciences Building, Edmonton, Alberta T6G 2H7, Canada
    Biochemistry 44:8068-77. 2005
  10. ncbi Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coli
    C P Tseng
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    J Bacteriol 178:1094-8. 1996

Research Grants

  1. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E COLI
    Robert Gunsalus; Fiscal Year: 1993
  2. REGULATION OF THE E COLI CYTOCHROME OXIDASE GENES
    Robert Gunsalus; Fiscal Year: 1993
  3. REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLE
    Robert Gunsalus; Fiscal Year: 2001
  4. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E COLI
    Robert Gunsalus; Fiscal Year: 2003
  5. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E. COLI
    Robert Gunsalus; Fiscal Year: 2007

Scientific Experts

  • Graham N George
  • Robert Gunsalus
  • Victor W T Cheng
  • R L Jack
  • Joel H Weiner
  • Shannon P Lubitz
  • Shawn M D Bearson
  • C P Tseng
  • J H Weiner
  • R A Rothery
  • A Dubini
  • F Sargent
  • Natalie C J Strynadka
  • Richard A Rothery
  • Michela G Bertero
  • T Palmer
  • A V Bogachev
  • Jeffrey A Albrecht
  • C A Trieber
  • R A Murtazina
  • V P Skulachev
  • J Albrecht
  • J L Johnson
  • K V Rajagopalan
  • J L Grant
  • P Cotter
  • D Sambasivarao
  • A K Hansen
  • P T Bilous
  • R Cammack
  • P A Cotter
  • D G Scraba
  • W F Anderson
  • C Trieber
  • S T Cole
  • D P MacIsaac
  • R E Bishop

Detail Information

Publications17

  1. ncbi Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli
    P A Cotter
    Department of Microbiology, University of California, Los Angeles
    J Bacteriol 171:3817-23. 1989
    ..Additionally, expression of dmsABC varied depending on the carbon source. Expression was highest when cells were grown on sorbitol...
  2. ncbi Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli
    P T Bilous
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Mol Microbiol 2:785-95. 1988
    ..The DMS C polypeptide is composed of eight segments of hydrophobic amino acids of appropriate length to cross the cytoplasmic membrane, suggesting that this subunit functions to anchor the enzyme to the membrane...
  3. ncbi Purification and properties of Escherichia coli dimethyl sulfoxide reductase, an iron-sulfur molybdoenzyme with broad substrate specificity
    J H Weiner
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Bacteriol 170:1505-10. 1988
    ..The enzyme displayed a very broad substrate specificity, reducing various N-oxide and sulfoxide compounds as well as chlorate and hydroxylamine...
  4. ncbi H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells
    A V Bogachev
    Department of Bioenergetics, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    J Bacteriol 178:6233-7. 1996
    ..These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force...
  5. ncbi Oxygen and nitrate-dependent regulation of dmsABC operon expression in Escherichia coli: sites for Fnr and NarL protein interactions
    Shawn M D Bearson
    Department of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, University of California, Los Angeles, CA 90095, USA
    BMC Microbiol 2:13. 2002
    ..Expression of the dmsABC genes that encode the membrane-associated DMSO/TMAO reductase is positively regulated during anaerobic conditions by the Fnr protein and negatively regulated by the NarL protein when nitrate is present...
  6. ncbi Alteration of the iron-sulfur cluster composition of Escherichia coli dimethyl sulfoxide reductase by site-directed mutagenesis
    R A Rothery
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biochemistry 30:8296-305. 1991
    ..The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [..
  7. ncbi The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC)
    Shannon P Lubitz
    CIHR Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Alta, T6G 2H7, Edmonton, Canada
    Arch Biochem Biophys 418:205-16. 2003
    ..ynfE and ynfF are paralogues of dmsA. ynfG and ynfH are paralogues of dmsB and dmsC, respectively. YnfI (dmsD) has no dms paralogue...
  8. ncbi Common principles in the biosynthesis of diverse enzymes
    R L Jack
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England, UK
    Biochem Soc Trans 33:105-7. 2005
    ....
  9. ncbi Investigation of the environment surrounding iron-sulfur cluster 4 of Escherichia coli dimethylsulfoxide reductase
    Victor W T Cheng
    Membrane Protein Research Group, Department of Biochemistry, University of Alberta, 474 Medical Sciences Building, Edmonton, Alberta T6G 2H7, Canada
    Biochemistry 44:8068-77. 2005
    ..The electron transfer subunit (DmsB) of Escherichia coli dimethylsulfoxide reductase contains four [4Fe-4S] clusters (FS1-FS4) with E(m) values between ..
  10. ncbi Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coli
    C P Tseng
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    J Bacteriol 178:1094-8. 1996
    ..These two transcriptional regulators coordinate the hierarchial control of respiratory pathway gene expression in E. coli to ensure the optimal use of oxygen in the cell environment...
  11. ncbi Multiple pathways of electron transfer in dimethyl sulfoxide reductase of Escherichia coli
    C A Trieber
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Biol Chem 269:7103-9. 1994
    ..were unable to support quinol oxidation although electron transfer from the quinol pool to the [Fe-S] centers in DmsB was normal...
  12. ncbi Effect of cell growth rate on expression of the anaerobic respiratory pathway operons frdABCD, dmsABC, and narGHJI of Escherichia coli
    C P Tseng
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024
    J Bacteriol 176:6599-605. 1994
    ..The cell appears to have many ways to adjust cell respiration in response to changes in cell growth conditions...
  13. ncbi Association of molybdopterin guanine dinucleotide with Escherichia coli dimethyl sulfoxide reductase: effect of tungstate and a mob mutation
    R A Rothery
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Bacteriol 177:2057-63. 1995
    ..These data support a model for Mo-MGD biosynthesis and assembly into DmsABC in which both metal chelation and nucleotide addition to MPT precede cofactor insertion...
  14. ncbi Electron paramagnetic resonance spectroscopic characterization of dimethyl sulfoxide reductase of Escherichia coli
    R Cammack
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    Biochemistry 29:8410-6. 1990
    ..acid sequence comparisons, that the molybdenum center is located in DmsA, while four iron-sulfur clusters are in DmsB. The midpoint potentials of the molybdenum and iron-sulfur clusters in the various preparations were determined by ..
  15. ncbi Organization of dimethyl sulfoxide reductase in the plasma membrane of Escherichia coli
    D Sambasivarao
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Bacteriol 172:5938-48. 1990
    ..electron microscopy of everted membrane vesicles and thin sections by using antibodies to the DmsABC, DmsA, DmsB subunits resulted in specific labeling of the cytoplasmic surface of the inner membrane...
  16. ncbi Dimethyl sulfoxide reductase of Escherichia coli: an investigation of function and assembly by use of in vivo complementation
    D Sambasivarao
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    J Bacteriol 173:5935-43. 1991
    ..The C-terminal region of DmsB appears to interact with the anchor peptide and facilitates the membrane assembly of the catalytic dimer.
  17. ncbi X-ray absorption spectroscopic characterization of the molybdenum site of Escherichia coli dimethyl sulfoxide reductase
    Graham N George
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    Inorg Chem 46:2-4. 2007
    ..Furthermore, our results suggest that the form found in vivo is the monooxobis(molybdopterin) species...

Research Grants33

  1. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E COLI
    Robert Gunsalus; Fiscal Year: 1993
    ..This oxygen phenomenon is involved in regulation of the electron transport process, photosynthesis and nitrogen fixation in a variety of bacterial species...
  2. REGULATION OF THE E COLI CYTOCHROME OXIDASE GENES
    Robert Gunsalus; Fiscal Year: 1993
    ..Finally, the effect of oxygen concentration and growth rate on cyoABCDE and cydAB gene expression will be tested to better understand the physiology of oxygen use by E. coli cells...
  3. REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLE
    Robert Gunsalus; Fiscal Year: 2001
    ..coli. These studies should provide a detailed understanding of how the cell coordinates pathways for electron and carbon flow within the cell during transition between oxygen rich and oxygen limiting conditions. ..
  4. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E COLI
    Robert Gunsalus; Fiscal Year: 2003
    ..These processes are also common to a large number of soil microorganisms that can adapt and respire anaerobically when oxygen becomes limited in the environment. ..
  5. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E. COLI
    Robert Gunsalus; Fiscal Year: 2007
    ..The Nar and Mode gene families will be identified by microarray analysis. Finally, the physiology of nitrate control will be examined by use of continuous culture methods. ..