flavivirus polyprotein gene

Summary

Gene Symbol: flavivirus polyprotein gene
Description: flavivirus polyprotein
Species: Dengue virus 1

Top Publications

  1. Lin Y, Liao C, Chen L, Yeh C, Liu C, Ma S, et al. Study of Dengue virus infection in SCID mice engrafted with human K562 cells. J Virol. 1998;72:9729-37 pubmed
    ..The system described herein offers an opportunity to explore DEN replication in vivo and to test various antiviral protocols in infected hosts...
  2. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 2002;40:376-81 pubmed
    ..Furthermore, NS1 circulation in the bloodstream of patients during the clinical phase of the disease suggests a contribution of the nonstructural protein to dengue virus pathogenesis...
  3. Goncalvez A, Purcell R, LAI C. Epitope determinants of a chimpanzee Fab antibody that efficiently cross-neutralizes dengue type 1 and type 2 viruses map to inside and in close proximity to fusion loop of the dengue type 2 virus envelope glycoprotein. J Virol. 2004;78:12919-28 pubmed publisher
    ..From the locations of these amino acids, Fab 1A5 appears to recognize a novel epitope that has not been mapped before with a flavivirus monoclonal antibody...
  4. Mu oz Jord n J, Laurent Rolle M, Ashour J, Mart nez Sobrido L, Ashok M, Lipkin W, et al. Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses. J Virol. 2005;79:8004-13 pubmed publisher
  5. Tan B, Fu J, Sugrue R. Characterization of the dengue virus envelope glycoprotein expressed in Pichia pastoris. Methods Mol Biol. 2007;379:163-76 pubmed
    ..The yeast-expressed E protein was treated with PNGase F, which showed that although the protein was modified by the addition of N-linked glycans, the recombinant expressed E protein was not hyperglycosylated. ..
  6. Cardosa M, Wang S, Sum M, Tio P. Antibodies against prM protein distinguish between previous infection with dengue and Japanese encephalitis viruses. BMC Microbiol. 2002;2:9 pubmed
    ..001. The use of flavivirus premembrane protein in seroepidemiological studies will be useful in determining what flaviviruses have circulated in a community...
  7. Thepparit C, Smith D. Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor. J Virol. 2004;78:12647-56 pubmed
    ..No inhibition of virus entry was seen with dengue virus serotypes 2, 3, or 4, demonstrating that the 37/67-kDa high-affinity laminin receptor is a serotype-specific receptor for dengue virus entry into liver cells. ..
  8. Mukhopadhyay S, Kuhn R, Rossmann M. A structural perspective of the flavivirus life cycle. Nat Rev Microbiol. 2005;3:13-22 pubmed publisher
    ..This review focuses mainly on structural studies of dengue virus...
  9. Hung S, Lee P, Chen H, Chen L, Kao C, King C. Analysis of the steps involved in Dengue virus entry into host cells. Virology. 1999;257:156-67 pubmed
    ..This suggests that cell surface heparan sulfate functions in both viral attachment and penetration of DEN-2 virus. In conclusion, our results further elucidated some aspects of the dengue virus entry process. ..

More Information

Publications92

  1. Roehrig J, Bolin R, Kelly R. Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica. Virology. 1998;246:317-28 pubmed publisher
    ..Domains A and B were spatially associated. As with tick-borne encephalitis virus, determination of domain C was more problematic; however, at least four epitopes had biochemical characteristics consistent with C-domain epitopes...
  2. Tassaneetrithep B, Burgess T, Granelli Piperno A, Trumpfheller C, Finke J, Sun W, et al. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J Exp Med. 2003;197:823-9 pubmed
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection. ..
  3. Purdy D, Chang G. Secretion of noninfectious dengue virus-like particles and identification of amino acids in the stem region involved in intracellular retention of envelope protein. Virology. 2005;333:239-50 pubmed publisher
  4. Zhang W, Chipman P, Corver J, Johnson P, Zhang Y, Mukhopadhyay S, et al. Visualization of membrane protein domains by cryo-electron microscopy of dengue virus. Nat Struct Biol. 2003;10:907-12 pubmed
    ..This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus. ..
  5. Cui T, Sugrue R, Xu Q, Lee A, Chan Y, Fu J. Recombinant dengue virus type 1 NS3 protein exhibits specific viral RNA binding and NTPase activity regulated by the NS5 protein. Virology. 1998;246:409-17 pubmed
    ..These data suggest that the NTPase activity of the NS3 protein may be regulated by the viral NS5 protein during virus replication. ..
  6. Duarte dos Santos C, Frenkiel M, Courageot M, Rocha C, Vazeille Falcoz M, Wien M, et al. Determinants in the envelope E protein and viral RNA helicase NS3 that influence the induction of apoptosis in response to infection with dengue type 1 virus. Virology. 2000;274:292-308 pubmed
    ..Our data suggest that identified amino acid substitutions in the envelope E protein and viral RNA helicase NS3 may influence DEN-1 virus pathogenicity by altering viral growth. ..
  7. Chen Y, Maguire T, Hileman R, Fromm J, Esko J, Linhardt R, et al. Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate. Nat Med. 1997;3:866-71 pubmed
    ..Similar motifs were also identified in the envelope proteins of other flaviviridae. Developing pharmaceuticals that inhibit target cell binding may be an effective strategy for treating flavivirus infections. ..
  8. Umareddy I, Chao A, Sampath A, Gu F, Vasudevan S. Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA. J Gen Virol. 2006;87:2605-14 pubmed publisher
    ..These results suggest that NS4B modulates dengue virus replication via its interaction with NS3...
  9. Despres P, Frenkiel M, Deubel V. Differences between cell membrane fusion activities of two dengue type-1 isolates reflect modifications of viral structure. Virology. 1993;196:209-19 pubmed
    ..Changes at positions 96, 297, and 379 map within two overlapping antigenic domains of protein E. These limited amino acid differences in the E protein could affect the biological properties and the antigenicity of the DEN virion. ..
  10. Zhang L, Mohan P, Padmanabhan R. Processing and localization of Dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5. J Virol. 1992;66:7549-54 pubmed
    ..Subcellular localization of the unprocessed precursor NS3-NS4A-NS4B-NS5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature NS5...
  11. Jones C, Ma L, Burgner J, Groesch T, Post C, Kuhn R. Flavivirus capsid is a dimeric alpha-helical protein. J Virol. 2003;77:7143-9 pubmed
    ..In contrast, the N terminus was found to be unstructured and could be removed without disrupting the structural integrity of the protein...
  12. Querenet M, Danjoy M, Mollereau B, Davoust N. Expression of dengue virus NS3 protein in Drosophila alters its susceptibility to infection. Fly (Austin). 2015;9:1-6 pubmed publisher
    ..In conclusion, we generated a new in vivo system to study the functional impact of DENV NS3 protein on the innate immune response. ..
  13. Lin C, Wan S, Cheng H, Lei H, Lin Y. Autoimmune pathogenesis in dengue virus infection. Viral Immunol. 2006;19:127-32 pubmed publisher
    ..Based on these findings, we hypothesize that anti-DV NS1 is involved in the pathogenesis of DF and DHF/DSS, and this may provide important information in dengue vaccine development...
  14. Chung K, Liszewski M, Nybakken G, Davis A, Townsend R, Fremont D, et al. West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H. Proc Natl Acad Sci U S A. 2006;103:19111-6 pubmed publisher
    ..Accordingly, extracellular NS1 may function to minimize immune system targeting of West Nile virus by decreasing complement recognition of infected cells...
  15. Miller S, Kastner S, Krijnse Locker J, B hler S, Bartenschlager R. The non-structural protein 4A of dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner. J Biol Chem. 2007;282:8873-82 pubmed publisher
    ..These results shed new light on the role of NS4A in the DV replication cycle and provide a model of how this protein induces membrane rearrangements and how this property may be regulated...
  16. Ma L, Jones C, Groesch T, Kuhn R, Post C. Solution structure of dengue virus capsid protein reveals another fold. Proc Natl Acad Sci U S A. 2004;101:3414-9 pubmed publisher
    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
  17. Dussart P, Labeau B, Lagathu G, Louis P, Nunes M, Rodrigues S, et al. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol. 2006;13:1185-9 pubmed publisher
    ..7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories...
  18. Lindenbach B, Rice C. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J Virol. 1999;73:4611-21 pubmed
    ..These findings suggest a potential mechanism for integrating NS1 into the cytoplasmic process of RNA replication...
  19. Tay M, Smith K, Ng I, Chan K, Zhao Y, Ooi E, et al. The C-terminal 18 Amino Acid Region of Dengue Virus NS5 Regulates its Subcellular Localization and Contains a Conserved Arginine Residue Essential for Infectious Virus Production. PLoS Pathog. 2016;12:e1005886 pubmed publisher
    ..Our data suggests an evolutionarily conserved function for NS5 Cter18, possibly in RNA interactions that are critical for replication, that is independent of its role in subcellular localization. ..
  20. Clum S, Ebner K, Padmanabhan R. Cotranslational membrane insertion of the serine proteinase precursor NS2B-NS3(Pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of NS2B. J Biol Chem. 1997;272:30715-23 pubmed
    ..Deletion of hydrophobic regions of NS2B, leaving only the conserved hydrophilic domain of 40 amino acids, resulted in highly efficient processing of the 2B-3 site in vitro in the absence of microsomal membranes...
  21. Wang S, He R, Anderson R. PrM- and cell-binding domains of the dengue virus E protein. J Virol. 1999;73:2547-51 pubmed
  22. Gebhard L, Iglesias N, Byk L, Filomatori C, De Maio F, Gamarnik A. A Proline-Rich N-Terminal Region of the Dengue Virus NS3 Is Crucial for Infectious Particle Production. J Virol. 2016;90:5451-61 pubmed publisher
    ..Based on our findings, we propose new ideas that include NS3 as a possible scaffold for the viral assembly process. ..
  23. Chandramouli S, Joseph J, Daudenarde S, Gatchalian J, Cornillez Ty C, Kuhn P. Serotype-specific structural differences in the protease-cofactor complexes of the dengue virus family. J Virol. 2010;84:3059-67 pubmed publisher
    ..We also report the identification of a 10-residue stretch within NS3pro that separates the substrate-binding function from the catalytic turnover rate of the enzyme. Implications for broad-spectrum drug discovery are discussed...
  24. Alcon LePoder S, Drouet M, Roux P, Frenkiel M, Arborio M, Durand Schneider A, et al. The secreted form of dengue virus nonstructural protein NS1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity. J Virol. 2005;79:11403-11 pubmed publisher
  25. McKee K, Bancroft W, Eckels K, Redfield R, Summers P, Russell P. Lack of attenuation of a candidate dengue 1 vaccine (45AZ5) in human volunteers. Am J Trop Med Hyg. 1987;36:435-42 pubmed
    ..The 45AZ5 virus is unacceptable as a vaccine candidate. This experience points out the uncertain relationship between in vitro viral growth characteristics and virulence factors for humans...
  26. Netsawang J, Noisakran S, Puttikhunt C, Kasinrerk W, Wongwiwat W, Malasit P, et al. Nuclear localization of dengue virus capsid protein is required for DAXX interaction and apoptosis. Virus Res. 2010;147:275-83 pubmed publisher
    ..This work is the first to demonstrate that nuclear localization of DENV C is required for DAXX interaction and apoptosis...
  27. Libraty D, Young P, Pickering D, Endy T, Kalayanarooj S, Green S, et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis. 2002;186:1165-8 pubmed publisher
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  28. Yon C, Teramoto T, Mueller N, Phelan J, Ganesh V, Murthy K, et al. Modulation of the nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase. J Biol Chem. 2005;280:27412-9 pubmed publisher
    ..Moreover, the conserved motif, 184RKRK, of NS3 played a crucial role in binding to RNA substrate and modulating the NTPase/RNA helicase and RTPase activities of NS3...
  29. Dechtawewat T, Paemanee A, Roytrakul S, Songprakhon P, Limjindaporn T, Yenchitsomanus P, et al. Mass spectrometric analysis of host cell proteins interacting with dengue virus nonstructural protein 1 in dengue virus-infected HepG2 cells. Biochim Biophys Acta. 2016;1864:1270-1280 pubmed publisher
    ..This study reports a novel set of DENV NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection. ..
  30. Cabrera Hernandez A, Thepparit C, Suksanpaisan L, Smith D. Dengue virus entry into liver (HepG2) cells is independent of hsp90 and hsp70. J Med Virol. 2007;79:386-92 pubmed publisher
    ..These results confirm a proposed role for GRP78 as a dengue virus serotype 2 receptor protein and suggest that the recently identified hsp90/70 complex does not play a role in dengue virus internalization into liver cells...
  31. Tajima S, Takasaki T, Kurane I. Characterization of Asn130-to-Ala mutant of dengue type 1 virus NS1 protein. Virus Genes. 2008;36:323-9 pubmed publisher
    ..Our results suggest that Asn130 of the DENV-1 NS1 is important for viral replication in both mammalian and mosquito cells. ..
  32. Ho L, Hung L, Weng C, Wu W, Chou P, Lin Y, et al. Dengue virus type 2 antagonizes IFN-alpha but not IFN-gamma antiviral effect via down-regulating Tyk2-STAT signaling in the human dendritic cell. J Immunol. 2005;174:8163-72 pubmed
    ..These observations provide very useful messages with physiological significance in investigation of the pathogenesis, the defense mechanisms of human hosts and the therapeutic considerations in DV infection...
  33. Noisakran S, Dechtawewat T, Avirutnan P, Kinoshita T, Siripanyaphinyo U, Puttikhunt C, et al. Association of dengue virus NS1 protein with lipid rafts. J Gen Virol. 2008;89:2492-500 pubmed publisher
    ..These results indicate an association of a minor subpopulation of NS1 with lipid rafts during dengue virus infection and suggest that modification of NS1, possibly lipidation, is required for raft association...
  34. Bhuvanakantham R, Li J, Tan T, Ng M. Human Sec3 protein is a novel transcriptional and translational repressor of flavivirus. Cell Microbiol. 2010;12:453-72 pubmed publisher
    ..This study also highlighted the antagonistic mechanism adopted by flavivirus C protein that can negatively regulate the formation of hSec3p-EF1alpha complex by sequestering hSec3p to establish successful infection...
  35. Puttikhunt C, Kasinrerk W, Srisa ad S, Duangchinda T, Silakate W, Moonsom S, et al. Production of anti-dengue NS1 monoclonal antibodies by DNA immunization. J Virol Methods. 2003;109:55-61 pubmed
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  36. Westaway E, Khromykh A, Kenney M, MacKenzie J, Jones M. Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus. Virology. 1997;234:31-41 pubmed publisher
    ..Dual IF with antibodies to double-stranded RNA showed that cytoplasmic locations of C and NS4B were apparently associated in part with the sites of viral RNA synthesis which were resistant to solubilization by Triton X-100...
  37. Tan B, Fu J, Sugrue R, Yap E, Chan Y, Tan Y. Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity. Virology. 1996;216:317-25 pubmed publisher
    ..The polymerase product was shown to be a negative-stranded RNA molecule, of template size, which forms a double-stranded complex with the template RNA...
  38. Hsieh S, Wu Y, Zou G, Nerurkar V, Shi P, Wang W. Highly conserved residues in the helical domain of dengue virus type 1 precursor membrane protein are involved in assembly, precursor membrane (prM) protein cleavage, and entry. J Biol Chem. 2014;289:33149-60 pubmed publisher
    ..The highly conserved nature of these residues suggests potential targets of antiviral strategy. ..
  39. Adikari T, Gomes L, Wickramasinghe N, Salimi M, Wijesiriwardana N, Kamaladasa A, et al. Dengue NS1 antigen contributes to disease severity by inducing interleukin (IL)-10 by monocytes. Clin Exp Immunol. 2016;184:90-100 pubmed publisher
    ..Therefore, our data show that dengue NS1 appears to contribute to pathogenesis of dengue infection by inducing IL-10 production by monocytes. ..
  40. Rey F. Dengue virus envelope glycoprotein structure: new insight into its interactions during viral entry. Proc Natl Acad Sci U S A. 2003;100:6899-901 pubmed publisher
  41. Brandt W, Chiewslip D, Harris D, Russell P. Partial purification and characterization of a dengue virus soluble complement-fixing antigen. J Immunol. 1970;105:1565-8 pubmed
  42. Bazan J, Fletterick R. Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology. 1989;171:637-9 pubmed
    ..Structural modeling of the putative flaviviral protease domain suggests amino acids that are crucial for catalytic activity and substrate binding...
  43. Sugrue R, Fu J, Howe J, Chan Y. Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles. J Gen Virol. 1997;78 ( Pt 8):1861-6 pubmed publisher
    ..This is the first report that expression of the structural genes of a flavivirus in yeast is able to generate particulate structures that resemble virions...
  44. Fu J, Tan B, Yap E, Chan Y, Tan Y. Full-length cDNA sequence of dengue type 1 virus (Singapore strain S275/90). Virology. 1992;188:953-8 pubmed
    ..Both the nucleotide and deduced amino acid sequences were compared with the partial sequence of DEN1 (Nauru Island) and complete sequences of DNE2 (Jamaica), DEN3 (H87), and DEN4 (Dominica) virus genomes...
  45. Li J, Lim S, Beer D, Patel V, Wen D, Tumanut C, et al. Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries. J Biol Chem. 2005;280:28766-74 pubmed publisher
    ..The specificities observed for dengue NS2B/NS3 have features in common with the physiological cleavage sites in the dengue polyprotein; however, all sites reveal previously unrecognized suboptimal sequences...
  46. Volk D, Lee Y, Li X, Thiviyanathan V, Gromowski G, Li L, et al. Solution structure of the envelope protein domain III of dengue-4 virus. Virology. 2007;364:147-54 pubmed publisher
    ..Important structural differences between DEN4-rED3 and ED3 domains of DEN2, DEN3 and other flaviviruses are discussed...
  47. Rico Hesse R. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology. 1990;174:479-93 pubmed
    ..This is the first genetic evidence of the existence of a sylvatic cycle of dengue virus, which is clearly distinct from outbreak viruses...
  48. Fonseca B, Pincus S, Shope R, Paoletti E, Mason P. Recombinant vaccinia viruses co-expressing dengue-1 glycoproteins prM and E induce neutralizing antibodies in mice. Vaccine. 1994;12:279-85 pubmed
  49. Steffens S, Thiel H, Behrens S. The RNA-dependent RNA polymerases of different members of the family Flaviviridae exhibit similar properties in vitro. J Gen Virol. 1999;80 ( Pt 10):2583-90 pubmed publisher
    ..Taken together, the RdRps of different members of the Flaviviridae were demonstrated to exhibit a common reactivity profile in vitro, typical of nucleic acid-polymerizing enzymes...
  50. LAI C, Monath T. Chimeric flaviviruses: novel vaccines against dengue fever, tick-borne encephalitis, and Japanese encephalitis. Adv Virus Res. 2003;61:469-509 pubmed
    ..The chimeric flavivirus strategy has led to the rapid development of novel live-attenuated vaccines against dengue, TBE, JE, and West Nile viruses...
  51. Martins I, Gomes Neto F, Faustino A, Carvalho F, Carneiro F, Bozza P, et al. The disordered N-terminal region of dengue virus capsid protein contains a lipid-droplet-binding motif. Biochem J. 2012;444:405-15 pubmed publisher
    ..Taken together, the results allowed the design of a peptide with inhibitory activity of C protein-LD binding, paving the way for new drug development approaches against dengue...
  52. Pryor M, Rawlinson S, Butcher R, Barton C, Waterhouse T, Vasudevan S, et al. Nuclear localization of dengue virus nonstructural protein 5 through its importin alpha/beta-recognized nuclear localization sequences is integral to viral infection. Traffic. 2007;8:795-807 pubmed publisher
    ..The results overall indicate that NS5 nuclear localization through the aNLS is integral to viral infection, with significant implications for other flaviviruses of medical importance, such as yellow fever and West Nile viruses...
  53. Rawlinson S, Pryor M, Wright P, Jans D. Dengue virus RNA polymerase NS5: a potential therapeutic target?. Curr Drug Targets. 2006;7:1623-38 pubmed
    ..This review examines the unique properties of NS5, including its functions, interactions, subcellular localisation and regulation, and looks at ways in which some of these may be exploited in our quest for effective drugs...
  54. Silva B, Sousa L, Gomes Ruiz A, Leite F, Teixeira M, da Fonseca F, et al. The dengue virus nonstructural protein 1 (NS1) increases NF-?B transcriptional activity in HepG2 cells. Arch Virol. 2011;156:1275-9 pubmed publisher
    ..Luciferase assays showed an increase in NF-?B transcriptional activities in NS1-expressing cells when compared to parental cells. NS1 may enhance NF-?B function in host cells and contribute to the pathogenesis of dengue...
  55. Erbel P, Schiering N, D Arcy A, Renatus M, Kroemer M, Lim S, et al. Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat Struct Mol Biol. 2006;13:372-3 pubmed publisher
    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...
  56. Crill W, Roehrig J. Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells. J Virol. 2001;75:7769-73 pubmed publisher
    ..These results extend our understanding of the structure-function relationships in the E glycoprotein of DEN virus and provide the first direct evidence that domain III encodes the primary flavivirus receptor-binding motif...
  57. Livingston P, Kurane I, Dai L, Okamoto Y, Lai C, Men R, et al. Dengue virus-specific, HLA-B35-restricted, human CD8+ cytotoxic T lymphocyte (CTL) clones. Recognition of NS3 amino acids 500 to 508 by CTL clones of two different serotype specificities. J Immunol. 1995;154:1287-95 pubmed
    ..These results indicate that CD8+ CTL clones that use different TCR and demonstrate two distinct serotype specificities recognize the same 9-mer peptide in the context of HLA-B35...
  58. Mateu G, Marchevsky R, Liprandi F, Bonaldo M, Coutinho E, Dieudonne M, et al. Construction and biological properties of yellow fever 17D/dengue type 1 recombinant virus. Trans R Soc Trop Med Hyg. 2007;101:289-98 pubmed
    ..This virus is a potential candidate to be included in a tetravalent DEN vaccine formulation. The availability of the cloned cDNA allows further structure/function studies on the viral envelope. ..
  59. Goncalvez A, Engle R, St Claire M, Purcell R, LAI C. Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention. Proc Natl Acad Sci U S A. 2007;104:9422-7 pubmed publisher
    ..Importantly, a 9-aa deletion at the N terminus of the CH(2) domain in the Fc region abrogated the enhancing activity...
  60. Fraser J, Rawlinson S, Heaton S, Jans D. Dynamic Nucleolar Targeting of Dengue Virus Polymerase NS5 in Response to Extracellular pH. J Virol. 2016;90:5797-5807 pubmed publisher
    ..Overall, this study identifies a unique subcellular trafficking event and suggests that DENV is able to detect and respond dynamically to environmental changes. ..
  61. MacKenzie J, Jones M, Young P. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology. 1996;220:232-40 pubmed publisher
    ..These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication...
  62. Lin C, Chiu S, Hsiao Y, Wan S, Lei H, Shiau A, et al. Expression of cytokine, chemokine, and adhesion molecules during endothelial cell activation induced by antibodies against dengue virus nonstructural protein 1. J Immunol. 2005;174:395-403 pubmed
    ..These results suggest the involvement of anti-DV NS1 Abs in the vasculopathy of DV infection...
  63. Shiu S, Ip K, Gould E, Chan K. Comparative analysis of the NS 1 gene sequences of dengue-1 viruses prototype Hawaii strain and Thai isolate TH-Sman, and determination of the intratypic variation of NS 1 protein among dengue-1 viruses. Arch Virol. 1993;131:447-54 pubmed
    ..1% to 3.1% difference in the NS 1 protein among dengue-1 strains, which is comparable to that reported for dengue-1 envelope protein (0.2% to 3.6% difference) but less than that of dengue-2 NS 1 protein (0.6% to 7.4% difference). ..
  64. Johnson A, Guirakhoo F, Roehrig J. The envelope glycoproteins of dengue 1 and dengue 2 viruses grown in mosquito cells differ in their utilization of potential glycosylation sites. Virology. 1994;203:241-9 pubmed publisher
    ..These DEN virus serotypes are thus heterogeneous in their use of glycosylation sites. We also determined by a lectin-binding assay that the attached carbohydrates for both viruses were likely to be of the high-mannose type...
  65. Patkar C, Kuhn R. Development of novel antivirals against flaviviruses. Novartis Found Symp. 2006;277:41-52; discussion 52-6, 71-3, 251-3 pubmed
  66. Clyde K, Harris E. RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replication. J Virol. 2006;80:2170-82 pubmed publisher
    ..While numerous regulatory elements have been identified in the untranslated regions of RNA viral genomes, we show that the cHP is a coding-region RNA element that directs start codon selection and is required for viral replication...
  67. Winkler G, Randolph V, Cleaves G, Ryan T, Stollar V. Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer. Virology. 1988;162:187-96 pubmed
    ..It is formed between 20 and 40 min after NS1 is synthesized and before the protein passes the Golgi apparatus. Both soluble and pelletable extracellular NS1 are also found as the high-molecular-weight form...
  68. Catteau A, Kalinina O, Wagner M, Deubel V, Courageot M, Despr s P. Dengue virus M protein contains a proapoptotic sequence referred to as ApoptoM. J Gen Virol. 2003;84:2781-93 pubmed publisher
    ..Defining the ApoptoM-activated signalling pathways leading to apoptosis will provide the basis for studying how the M protein might play a key role in the fate of the flavivirus-infected cells...
  69. Mercado Curiel R, Esquinca Avil s H, Tovar R, D az Badillo A, Camacho Nuez M. The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol. 2006;6:85 pubmed publisher
    ..aegypti midgut. Our results strongly suggest that R67 and R80 are receptors for the four serotypes of dengue virus in the midgut cells of Ae. aegypti and in C6/36 Ae. albopictus cells...
  70. Kuno G, Vorndam A, Gubler D, G mez I. Study of anti-dengue NS1 antibody by western blot. J Med Virol. 1990;32:102-8 pubmed
    ..No difference was observed, however, in Indonesian patients with secondary infection who had dengue fever or DHF...
  71. Jacobs M, Robinson P, Bletchly C, MacKenzie J, Young P. Dengue virus nonstructural protein 1 is expressed in a glycosyl-phosphatidylinositol-linked form that is capable of signal transduction. FASEB J. 2000;14:1603-10 pubmed
    ..Signal transduction by a GPI-anchored viral antigen interacting with a specific antibody that it induces is a new concept in the pathogenesis of viral disease...
  72. Preugschat F, Strauss J. Processing of nonstructural proteins NS4A and NS4B of dengue 2 virus in vitro and in vivo. Virology. 1991;185:689-97 pubmed
  73. Thullier P, Lafaye P, M gret F, Deubel V, Jouan A, Mazi J. A recombinant Fab neutralizes dengue virus in vitro. J Biotechnol. 1999;69:183-90 pubmed
    ..e. nanomolar. Fab 4E11 neutralization capacity was found between 8 and 4-times or less lower than that of mAb 4E11, depending on serotypes, thus the Fab could have a smaller antiviral activity than the mAb in vitro...
  74. Parida P, Yadav R, Sarma K. Insight into structural organization and protein-protein interaction of non structural 3 (NS3) proteins from dengue serotypes. Curr Pharm Biotechnol. 2014;15:156-72 pubmed
    ..Suface and interface residues were observed along with hydrogen and hydrophobic interaction. The conserved residues forming hydrogen interaction of NRBP with DENV1-4 serotypes were found to be GLN 305, SER 363 and GLN 379. ..
  75. Falconar A, Young P. Immunoaffinity purification of native dimer forms of the flavivirus non-structural glycoprotein, NS1. J Virol Methods. 1990;30:323-32 pubmed
    ..In moderately sized virus growth experiments, greater than 1 mg quantities of purified NS1 were obtained...
  76. Shah M, Wadood A, RAHMAN Z, Husnain T. Interaction and inhibition of dengue envelope glycoprotein with mammalian receptor DC-sign, an in-silico approach. PLoS ONE. 2013;8:e59211 pubmed publisher
  77. Falconar A, Young P. Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1. J Gen Virol. 1991;72 ( Pt 4):961-5 pubmed publisher
  78. Ishak H, Takegami T, Kamimura K, Funada H. Comparative sequences of two type 1 dengue virus strains possessing different growth characteristics in vitro. Microbiol Immunol. 2001;45:327-31 pubmed
    ..These substitutions might be correlated to their different growth characteristics in vitro. ..
  79. Yocupicio Monroy M, Padmanabhan R, Medina F, Del Angel R. Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells. Virology. 2007;357:29-40 pubmed publisher
    ..Furthermore, the presence of La protein in an in vitro replication system inhibited RNA synthesis in a dose-dependent manner, suggesting that La protein plays an important role in dengue virus replicative cycle...
  80. Vinayagamoorthy T, Mulatz K, Drebot M, Hodkinson R. Molecular typing of West Nile Virus, Dengue, and St. Louis encephalitis using multiplex sequencing. J Mol Diagn. 2005;7:152-9 pubmed
  81. PremKumar A, Horan C, Gage P. Dengue virus M protein C-terminal peptide (DVM-C) forms ion channels. J Membr Biol. 2005;204:33-8 pubmed publisher
    ..Ion channels may play an important role in the life cycle of many viruses and drugs that block these channels may prove to be useful antiviral agents...
  82. Khunchai S, Junking M, Suttitheptumrong A, Yasamut U, Sawasdee N, Netsawang J, et al. Interaction of dengue virus nonstructural protein 5 with Daxx modulates RANTES production. Biochem Biophys Res Commun. 2012;423:398-403 pubmed publisher
    ..This work demonstrates the interaction between DENV NS5 and Daxx and the role of the interaction on the modulation of RANTES production...
  83. Lin C, Lei H, Shiau A, Liu H, Yeh T, Chen S, et al. Endothelial cell apoptosis induced by antibodies against dengue virus nonstructural protein 1 via production of nitric oxide. J Immunol. 2002;169:657-64 pubmed
    ..Endothelial cell damage may cause vascular leakage that contributes to the pathogenesis of dengue disease...