DV1_gp1

Summary

Gene Symbol: DV1_gp1
Description: polyprotein
Species: Dengue virus 1

Top Publications

  1. pmc Study of Dengue virus infection in SCID mice engrafted with human K562 cells
    Y L Lin
    Institute of Biomedical Sciences, Academia Sinica, National Taiwan University, Taipei, Taiwan, Republic of China
    J Virol 72:9729-37. 1998
  2. pmc Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections
    Sophie Alcon
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, France
    J Clin Microbiol 40:376-81. 2002
  3. pmc Epitope determinants of a chimpanzee Fab antibody that efficiently cross-neutralizes dengue type 1 and type 2 viruses map to inside and in close proximity to fusion loop of the dengue type 2 virus envelope glycoprotein
    Ana P Goncalvez
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6349, 50 South Dr, MSC 8009, Bethesda, MD 20892, USA
    J Virol 78:12919-28. 2004
  4. pmc Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses
    Jorge L Munoz-Jordan
    Department of Microbiology, Box 1124 Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, New York 10029, USA
    J Virol 79:8004-13. 2005
  5. ncbi Characterization of the dengue virus envelope glycoprotein expressed in Pichia pastoris
    Boon Huan Tan
    Virology Group Defence Medical and Environmental Research Institute, DSO National Laboratories, Singapore
    Methods Mol Biol 379:163-76. 2007
  6. pmc DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells
    Boonrat Tassaneetrithep
    Division of Retrovirology, Walter Reed Army Institite of Research and Henry M Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA
    J Exp Med 197:823-9. 2003
  7. ncbi Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate
    Y Chen
    Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA
    Nat Med 3:866-71. 1997
  8. ncbi Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA
    Indira Umareddy
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos Building, Singapore
    J Gen Virol 87:2605-14. 2006
  9. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
  10. pmc Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor
    Chutima Thepparit
    Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, 25 25 Phuttamontol Sai 4, Salaya, Nakorn Pathom, Thailand 73170
    J Virol 78:12647-56. 2004

Research Grants

Scientific Experts

  • Ricardo F Mercado-Curiel
  • P Despres
  • J T Roehrig
  • B Puri
  • Philippe Dussart
  • Phaik Hooi Tio
  • Ana P Goncalvez
  • Shigeru Tajima
  • Yi Ling Lin
  • I Phillips
  • Mary A Marovich
  • A PremKumar
  • Felix A Rey
  • Shan Ling Hung
  • Mary Jane Cardosa
  • Daniel H Libraty
  • Wayne D Crill
  • M Flamand
  • F Guirakhoo
  • Chiou Feng Lin
  • Subhash G Vasudevan
  • Chunya Puttikhunt
  • Richard J Kuhn
  • Jun Li
  • Watchara Kasinrerk
  • Prida Malasit
  • Yorgo Modis
  • Chutima Thepparit
  • Ivo C Martins
  • Cécile Bussetta
  • Huan Yao Lei
  • Suchetana Mukhopadhyay
  • Breno M Silva
  • Janjuree Netsawang
  • Raghavan Bhuvanakantham
  • Yue Chen
  • Vinod Nayak
  • Pa thai Yenchitsomanus
  • Sansanee Noisakran
  • Boon Huan Tan
  • Arturo Cabrera-Hernandez
  • David E Volk
  • Olesia Lisova
  • Martha Yocupicio-Monroy
  • Sergey A Shiryaev
  • Melinda J Pryor
  • Paul Erbel
  • Panisadee Avirutnan
  • Chinmay G Patkar
  • Carl W Davis
  • Karen Clyde
  • R Padmanabhan
  • Indira Umareddy
  • Shu Wen Wan
  • Duncan R Smith
  • Siew Pheng Lim
  • David E Purdy
  • Sophie Alcon-LePoder
  • Thuraiayah Vinayagamoorthy
  • Jorge L Munoz-Jordan
  • Changsuek Yon
  • Nopporn Sittisombut
  • David A Jans
  • Atchareeya A-Nuegoonpipat
  • R J Sugrue
  • Lixin Ma
  • Michael G Rossmann
  • Shun Hua Chen
  • Hsiao Sheng Liu
  • Trai Ming Yeh
  • Shu Chen Chiu
  • Ai Li Shiau
  • Ching Chuan Liu
  • Wei Zhang
  • Boonrat Tassaneetrithep
  • CHING JUH LAI
  • J Fu
  • Raphaele Germi
  • Y C Chan
  • Andrew J Brooks
  • Sophie Alcon
  • Miguel A R B Castanho
  • Ronaldo Mohana-Borges
  • Kyung H Choi
  • Francisco Gomes-Neto
  • Filomena A Carvalho
  • Patricia T Bozza
  • Nuno C Santos
  • Fabiana A Carneiro
  • Andrea T Da Poian

Detail Information

Publications84

  1. pmc Study of Dengue virus infection in SCID mice engrafted with human K562 cells
    Y L Lin
    Institute of Biomedical Sciences, Academia Sinica, National Taiwan University, Taipei, Taiwan, Republic of China
    J Virol 72:9729-37. 1998
    ..The system described herein offers an opportunity to explore DEN replication in vivo and to test various antiviral protocols in infected hosts...
  2. pmc Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections
    Sophie Alcon
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, France
    J Clin Microbiol 40:376-81. 2002
    ..Furthermore, NS1 circulation in the bloodstream of patients during the clinical phase of the disease suggests a contribution of the nonstructural protein to dengue virus pathogenesis...
  3. pmc Epitope determinants of a chimpanzee Fab antibody that efficiently cross-neutralizes dengue type 1 and type 2 viruses map to inside and in close proximity to fusion loop of the dengue type 2 virus envelope glycoprotein
    Ana P Goncalvez
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6349, 50 South Dr, MSC 8009, Bethesda, MD 20892, USA
    J Virol 78:12919-28. 2004
    ..From the locations of these amino acids, Fab 1A5 appears to recognize a novel epitope that has not been mapped before with a flavivirus monoclonal antibody...
  4. pmc Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses
    Jorge L Munoz-Jordan
    Department of Microbiology, Box 1124 Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, New York 10029, USA
    J Virol 79:8004-13. 2005
    ....
  5. ncbi Characterization of the dengue virus envelope glycoprotein expressed in Pichia pastoris
    Boon Huan Tan
    Virology Group Defence Medical and Environmental Research Institute, DSO National Laboratories, Singapore
    Methods Mol Biol 379:163-76. 2007
    ..The yeast-expressed E protein was treated with PNGase F, which showed that although the protein was modified by the addition of N-linked glycans, the recombinant expressed E protein was not hyperglycosylated...
  6. pmc DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells
    Boonrat Tassaneetrithep
    Division of Retrovirology, Walter Reed Army Institite of Research and Henry M Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA
    J Exp Med 197:823-9. 2003
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection...
  7. ncbi Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate
    Y Chen
    Department of Internal Medicine, University of Michigan, Ann Arbor 48109, USA
    Nat Med 3:866-71. 1997
    ..Similar motifs were also identified in the envelope proteins of other flaviviridae. Developing pharmaceuticals that inhibit target cell binding may be an effective strategy for treating flavivirus infections...
  8. ncbi Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA
    Indira Umareddy
    Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05 01 Chromos Building, Singapore
    J Gen Virol 87:2605-14. 2006
    ..These results suggest that NS4B modulates dengue virus replication via its interaction with NS3...
  9. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
    ..This review focuses mainly on structural studies of dengue virus...
  10. pmc Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor
    Chutima Thepparit
    Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, 25 25 Phuttamontol Sai 4, Salaya, Nakorn Pathom, Thailand 73170
    J Virol 78:12647-56. 2004
    ..No inhibition of virus entry was seen with dengue virus serotypes 2, 3, or 4, demonstrating that the 37/67-kDa high-affinity laminin receptor is a serotype-specific receptor for dengue virus entry into liver cells...
  11. ncbi Analysis of the steps involved in Dengue virus entry into host cells
    S L Hung
    Institute of Oral Biology, National Yang Ming University, Taipei, Taiwan
    Virology 257:156-67. 1999
    ..This suggests that cell surface heparan sulfate functions in both viral attachment and penetration of DEN-2 virus. In conclusion, our results further elucidated some aspects of the dengue virus entry process...
  12. ncbi Visualization of membrane protein domains by cryo-electron microscopy of dengue virus
    Wei Zhang
    Department of Biological Sciences, Lilly Hall, 915 W State Street, Purdue University, West Lafayette, Indiana 47907, USA
    Nat Struct Biol 10:907-12. 2003
    ..This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus...
  13. ncbi Determinants in the envelope E protein and viral RNA helicase NS3 that influence the induction of apoptosis in response to infection with dengue type 1 virus
    C N Duarte dos Santos
    Departmento de Bioquimica e Biologia Molecular, Laboratorio de Expressao e Regulaçao Genica, Rio de Janeiro, R J, Brazil
    Virology 274:292-308. 2000
    ..Our data suggest that identified amino acid substitutions in the envelope E protein and viral RNA helicase NS3 may influence DEN-1 virus pathogenicity by altering viral growth...
  14. pmc Antibodies against prM protein distinguish between previous infection with dengue and Japanese encephalitis viruses
    Mary Jane Cardosa
    Institute of Health and Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
    BMC Microbiol 2:9. 2002
    ....
  15. ncbi Recombinant dengue virus type 1 NS3 protein exhibits specific viral RNA binding and NTPase activity regulated by the NS5 protein
    T Cui
    Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore
    Virology 246:409-17. 1998
    ..These data suggest that the NTPase activity of the NS3 protein may be regulated by the viral NS5 protein during virus replication...
  16. doi The dengue virus nonstructural protein 1 (NS1) increases NF-κB transcriptional activity in HepG2 cells
    Breno M Silva
    Grupo de Transdução de Sinal, Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270 901, Brazil
    Arch Virol 156:1275-9. 2011
    ..Luciferase assays showed an increase in NF-κB transcriptional activities in NS1-expressing cells when compared to parental cells. NS1 may enhance NF-κB function in host cells and contribute to the pathogenesis of dengue...
  17. ncbi Lack of attenuation of a candidate dengue 1 vaccine (45AZ5) in human volunteers
    K T McKee
    Am J Trop Med Hyg 36:435-42. 1987
    ..The 45AZ5 virus is unacceptable as a vaccine candidate. This experience points out the uncertain relationship between in vitro viral growth characteristics and virulence factors for humans...
  18. ncbi Secretion of noninfectious dengue virus-like particles and identification of amino acids in the stem region involved in intracellular retention of envelope protein
    David E Purdy
    Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, PO Box 2087, Fort Collins, CO 80522, USA
    Virology 333:239-50. 2005
    ....
  19. ncbi Differences between cell membrane fusion activities of two dengue type-1 isolates reflect modifications of viral structure
    P Despres
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, Paris, France
    Virology 196:209-19. 1993
    ..Changes at positions 96, 297, and 379 map within two overlapping antigenic domains of protein E. These limited amino acid differences in the E protein could affect the biological properties and the antigenicity of the DEN virion...
  20. ncbi Endothelial cell apoptosis induced by antibodies against dengue virus nonstructural protein 1 via production of nitric oxide
    Chiou Feng Lin
    Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China
    J Immunol 169:657-64. 2002
    ..Endothelial cell damage may cause vascular leakage that contributes to the pathogenesis of dengue disease...
  21. ncbi The interdomain region of dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin beta 1 and importin alpha/beta-recognized nuclear localization signals
    Andrew J Brooks
    Department of Biochemistry and Molecular Biology, James Cook University, Queensland 4811, Australia
    J Biol Chem 277:36399-407. 2002
    ..Intriguingly, the bNLS is highly conserved among Dengue and related flaviviruses, implying a general role for the region and importin beta in the infectious cycle...
  22. ncbi Full-length cDNA sequence of dengue type 1 virus (Singapore strain S275/90)
    J Fu
    Institute of Molecular and Cell Biology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge
    Virology 188:953-8. 1992
    ..Both the nucleotide and deduced amino acid sequences were compared with the partial sequence of DEN1 (Nauru Island) and complete sequences of DNE2 (Jamaica), DEN3 (H87), and DEN4 (Dominica) virus genomes...
  23. ncbi Sustained transmission of dengue virus type 1 in the Pacific due to repeated introductions of different Asian strains
    Atchareeya A-Nuegoonpipat
    National Institute of Health, Department of Medical Science, Bangkok, Thailand
    Virology 329:505-12. 2004
    ..There was no evidence that the introduction of these viruses into the Pacific was associated with any adaptive changes in the E protein of the viruses...
  24. ncbi Dengue-1 virus envelope glycoprotein gene expressed in recombinant baculovirus elicits virus-neutralizing antibody in mice and protects them from virus challenge
    R Putnak
    Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC
    Am J Trop Med Hyg 45:159-67. 1991
    ..A dose/response effect was observed, with increasing amounts of recombinant antigen leading to increased survival. These results demonstrate the utility of baculovirus for producing immunogens against DEN-1 virus...
  25. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell...
  26. pmc Crystal structure of dengue virus type 1 envelope protein in the postfusion conformation and its implications for membrane fusion
    Vinod Nayak
    Department of Molecular Biophysics and Biochemistry, The Bass Center for Structural Biology, Yale University, New Haven, Connecticut 06520, USA
    J Virol 83:4338-44. 2009
    ..In the fusion loop, Phe-108 adopts a distinct conformation, forming additional trimer contacts and filling the bowl-shaped concavity observed at the tip of the DEN-2 sE trimer...
  27. ncbi The envelope glycoproteins of dengue 1 and dengue 2 viruses grown in mosquito cells differ in their utilization of potential glycosylation sites
    A J Johnson
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522
    Virology 203:241-9. 1994
    ..These DEN virus serotypes are thus heterogeneous in their use of glycosylation sites. We also determined by a lectin-binding assay that the attached carbohydrates for both viruses were likely to be of the high-mannose type...
  28. pmc Dengue virus nonstructural protein 5 adopts multiple conformations in solution
    Cécile Bussetta
    Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555 0647, USA
    Biochemistry 51:5921-31. 2012
    ..We interpret the multiple conformations of NS5 observed in solution as resulting from weak interactions between the two NS5 domains and flexibility of the linker in the absence of other components of the replication complex...
  29. ncbi Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques
    K Raviprakash
    Virology Program, Infectious Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Rm 3N71, Silver Spring, MD 20910 7500, USA
    J Gen Virol 81:1659-67. 2000
    ..Vaccine-induced antibody had an avidity index similar to that of antibody induced by virus infection; however, no clear correlation was apparent between antibody avidity and virus neutralization titres...
  30. pmc A single amino acid substitution in the envelope protein of chimeric yellow fever-dengue 1 vaccine virus reduces neurovirulence for suckling mice and viremia/viscerotropism for monkeys
    F Guirakhoo
    Acambis, Inc, 38 Sidney St, Cambridge, MA 02139, USA
    J Virol 78:9998-10008. 2004
    ..These changes may be responsible for virus attenuation through a change in the pH threshold for virus envelope fusion with the host cell membrane...
  31. pmc Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3
    Phaik Hooi Tio
    Institute of Health and Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
    Virol J 2:25. 2005
    ....
  32. ncbi Expression of cytokine, chemokine, and adhesion molecules during endothelial cell activation induced by antibodies against dengue virus nonstructural protein 1
    Chiou Feng Lin
    Department of Micribiology and Immunology, National Cheng Kung University Medical College, 1 University Road, Tainan 701, Taiwan
    J Immunol 174:395-403. 2005
    ..These results suggest the involvement of anti-DV NS1 Abs in the vasculopathy of DV infection...
  33. ncbi Recombinant vaccinia viruses co-expressing dengue-1 glycoproteins prM and E induce neutralizing antibodies in mice
    B A Fonseca
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06510
    Vaccine 12:279-85. 1994
    ....
  34. ncbi Dengue virus nonstructural protein 1 is expressed in a glycosyl-phosphatidylinositol-linked form that is capable of signal transduction
    M G Jacobs
    Department of Paediatrics, Imperial College School of Medicine, Norfolk Place, London W2 1PG, U K
    FASEB J 14:1603-10. 2000
    ..Signal transduction by a GPI-anchored viral antigen interacting with a specific antibody that it induces is a new concept in the pathogenesis of viral disease...
  35. ncbi Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity
    B H Tan
    Institute of Molecular and Cell Biology, National University of Singapore
    Virology 216:317-25. 1996
    ..The polymerase product was shown to be a negative-stranded RNA molecule, of template size, which forms a double-stranded complex with the template RNA...
  36. pmc Human isolates of dengue type 1 virus induce apoptosis in mouse neuroblastoma cells
    P Despres
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, Paris, France
    J Virol 70:4090-6. 1996
    ..Accumulation of viral proteins in the endoplasmic reticulum may induce stress and thereby activate the apoptotic pathway in mouse neuroblastoma cells...
  37. doi Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1
    Yue Chen
    Center for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, No 253 Gong ye da dao zhong, Guangzhou 510282, People s Republic of China
    Virology 398:290-8. 2010
    ..These novel immunodominant serotype- and group-specific B-cell epitopes of DENV NS1 may aid the development of new dengue vaccines and diagnostic assays...
  38. ncbi Molecular evolution and distribution of dengue viruses type 1 and 2 in nature
    R Rico-Hesse
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510
    Virology 174:479-93. 1990
    ..This is the first genetic evidence of the existence of a sylvatic cycle of dengue virus, which is clearly distinct from outbreak viruses...
  39. ncbi The antigenic structure of dengue type 1 virus envelope and NS1 proteins expressed in Escherichia coli
    P W Mason
    Yale Arbovirus Research Unit, Yale University School of Medicine, New Haven, Connecticut 06510
    J Gen Virol 71:2107-14. 1990
    ..The antibodies elicited by the E fusion protein reacted with a portion of the protein containing domain II, whereas antibodies elicited by the NS1 fusion protein did not react with the antigenic determinants defined by our MAbs...
  40. ncbi Passive protection studies in mice with monoclonal antibodies directed against the non-structural protein NS3 of dengue 1 virus
    C H Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge
    J Gen Virol 71:745-9. 1990
    ..Monoclonal antibodies to NS3 of dengue 1 virus are able to increase the survival time of mice challenged with a lethal dose of dengue 1 virus, although the mechanism remains to be defined...
  41. doi The disordered N-terminal region of dengue virus capsid protein contains a lipid-droplet-binding motif
    Ivo C Martins
    Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941 902, Brazil
    Biochem J 444:405-15. 2012
    ..Taken together, the results allowed the design of a peptide with inhibitory activity of C protein-LD binding, paving the way for new drug development approaches against dengue...
  42. ncbi Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement
    Panisadee Avirutnan
    Medical Molecular Biology Unit, Siriraj Hospital, Mahidol University, Bangkok, Thailand
    J Infect Dis 193:1078-88. 2006
    ..Thirty years ago, complement activation was proposed to be a key underlying event, but the cause of complement activation has remained unknown...
  43. pmc Variable surface epitopes in the crystal structure of dengue virus type 3 envelope glycoprotein
    Yorgo Modis
    Children s Hospital, Enders 673, 320 Longwood Ave, Boston, MA 02115, USA
    J Virol 79:1223-31. 2005
    ....
  44. doi Human Sec3 protein is a novel transcriptional and translational repressor of flavivirus
    Raghavan Bhuvanakantham
    Flavivirology Laboratory, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597
    Cell Microbiol 12:453-72. 2010
    ..This study also highlighted the antagonistic mechanism adopted by flavivirus C protein that can negatively regulate the formation of hSec3p-EF1alpha complex by sequestering hSec3p to establish successful infection...
  45. pmc Molecular typing of West Nile Virus, Dengue, and St. Louis encephalitis using multiplex sequencing
    Thuraiayah Vinayagamoorthy
    Bio ID Diagnostic Inc, Saskatoon, Saskatchewan, Canada
    J Mol Diagn 7:152-9. 2005
    ....
  46. ncbi Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries
    Jun Li
    Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
    J Biol Chem 280:28766-74. 2005
    ..The specificities observed for dengue NS2B/NS3 have features in common with the physiological cleavage sites in the dengue polyprotein; however, all sites reveal previously unrecognized suboptimal sequences...
  47. ncbi Autoimmune pathogenesis in dengue virus infection
    Chiou Feng Lin
    Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan
    Viral Immunol 19:127-32. 2006
    ..Based on these findings, we hypothesize that anti-DV NS1 is involved in the pathogenesis of DF and DHF/DSS, and this may provide important information in dengue vaccine development...
  48. ncbi Development of novel antivirals against flaviviruses
    Chinmay G Patkar
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Novartis Found Symp 277:41-52; discussion 52-6, 71-3, 251-3. 2006
    ....
  49. pmc RNA secondary structure in the coding region of dengue virus type 2 directs translation start codon selection and is required for viral replication
    Karen Clyde
    Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, 94720 7360, USA
    J Virol 80:2170-82. 2006
    ..While numerous regulatory elements have been identified in the untranslated regions of RNA viral genomes, we show that the cHP is a coding-region RNA element that directs start codon selection and is required for viral replication...
  50. ncbi Mosquito La protein binds to the 3' untranslated region of the positive and negative polarity dengue virus RNAs and relocates to the cytoplasm of infected cells
    Martha Yocupicio-Monroy
    Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico, Mexico City 03100, Mexico
    Virology 357:29-40. 2007
    ..Furthermore, the presence of La protein in an in vitro replication system inhibited RNA synthesis in a dose-dependent manner, suggesting that La protein plays an important role in dengue virus replicative cycle...
  51. pmc The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells
    Ricardo F Mercado-Curiel
    Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Ave Instituto Politécnico Nacional 2508 Col San Pedro Zacatenco, C P 07360, Mexico, D F, Mexico
    BMC Microbiol 6:85. 2006
    ..Determination of which of them serve as bona fide receptors for this virus in the vector may be relevant to treating DENV infection and in designing control strategies...
  52. pmc West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection
    Carl W Davis
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    J Virol 80:1290-301. 2006
    ..We believe this is the first report of a pathogen interacting more efficiently with DC-SIGNR than with DC-SIGN. Our results should lead to the discovery of new mechanisms by which these well-studied lectins discriminate among ligands...
  53. ncbi Dengue virus entry into liver (HepG2) cells is independent of hsp90 and hsp70
    Arturo Cabrera-Hernandez
    Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Nakorn Pathom, Thailand
    J Med Virol 79:386-92. 2007
    ..These results confirm a proposed role for GRP78 as a dengue virus serotype 2 receptor protein and suggest that the recently identified hsp90/70 complex does not play a role in dengue virus internalization into liver cells...
  54. ncbi Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus
    Paul Erbel
    Novartis Institutes for BioMedical Research, Protease Platform, 4002 Basel, Switzerland
    Nat Struct Mol Biol 13:372-3. 2006
    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...
  55. ncbi Mapping to completeness and transplantation of a group-specific, discontinuous, neutralizing epitope in the envelope protein of dengue virus
    Olesia Lisova
    Unit of Molecular Prevention and Therapy of Human Diseases CNRS URA3012, Institut Pasteur, 28 rue Docteur Roux, F 75724 Paris Cedex 15, France
    J Gen Virol 88:2387-97. 2007
    ..The epitope straddles residues of ED3 that are involved in virulence, e.g. Asn/Asp390. These results define the epitope of mAb4E11 as an antigenic signature of the DENV group and suggest mechanisms for its neutralization potency...
  56. ncbi Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica
    J T Roehrig
    Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
    Virology 246:317-28. 1998
    ..Domains A and B were spatially associated. As with tick-borne encephalitis virus, determination of domain C was more problematic; however, at least four epitopes had biochemical characteristics consistent with C-domain epitopes...
  57. ncbi Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus
    E G Westaway
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Virology 234:31-41. 1997
    ..Dual IF with antibodies to double-stranded RNA showed that cytoplasmic locations of C and NS4B were apparently associated in part with the sites of viral RNA synthesis which were resistant to solubilization by Triton X-100...
  58. ncbi A recombinant Fab neutralizes dengue virus in vitro
    P Thullier
    Laboratoire d Ingénierie des Anticorps, Institut Pasteur, Paris, France
    J Biotechnol 69:183-90. 1999
    ..e. nanomolar. Fab 4E11 neutralization capacity was found between 8 and 4-times or less lower than that of mAb 4E11, depending on serotypes, thus the Fab could have a smaller antiviral activity than the mAb in vitro...
  59. pmc Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses
    E Wang
    Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas 77555 0609, USA
    J Virol 74:3227-34. 2000
    ..Analysis of envelope protein amino acid changes predicted to have accompanied endemic/epidemic emergence suggested a role for domain III in adaptation to new mosquito and/or human hosts...
  60. pmc Infection of human cells by dengue virus is modulated by different cell types and viral strains
    M S Diamond
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    J Virol 74:7814-23. 2000
    ..Thus, depending on the cell type and viral isolate, blocks that limit the production of infectious virus in vitro may occur at distinct steps in the pathway of cellular infection...
  61. ncbi Comparative analysis of the NS 1 gene sequences of dengue-1 viruses prototype Hawaii strain and Thai isolate TH-Sman, and determination of the intratypic variation of NS 1 protein among dengue-1 viruses
    S Y Shiu
    Department of Microbiology, University of Hong Kong
    Arch Virol 131:447-54. 1993
    ..1% to 3.1% difference in the NS 1 protein among dengue-1 strains, which is comparable to that reported for dengue-1 envelope protein (0.2% to 3.6% difference) but less than that of dengue-2 NS 1 protein (0.6% to 7.4% difference)...
  62. ncbi Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses
    J F Bazan
    Department of Biophysics, University of California, Berkeley 94720
    Virology 171:637-9. 1989
    ..Structural modeling of the putative flaviviral protease domain suggests amino acids that are crucial for catalytic activity and substrate binding...
  63. ncbi High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever
    Daniel H Libraty
    Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655, USA
    J Infect Dis 186:1165-8. 2002
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  64. ncbi Processing of nonstructural proteins NS4A and NS4B of dengue 2 virus in vitro and in vivo
    F Preugschat
    Division of Biology, California Institute of Technology, Pasadena 91125
    Virology 185:689-97. 1991
    ....
  65. ncbi Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1
    A K Falconar
    Wolfson Molecular Biology Unit, London School of Hygiene and Tropical Medicine, U K
    J Gen Virol 72:961-5. 1991
    ....
  66. ncbi Dengue virus-specific, HLA-B35-restricted, human CD8+ cytotoxic T lymphocyte (CTL) clones. Recognition of NS3 amino acids 500 to 508 by CTL clones of two different serotype specificities
    P G Livingston
    Department of Medicine, University of Massachusetts Medical Center, Worcester 01655
    J Immunol 154:1287-95. 1995
    ..These results indicate that CD8+ CTL clones that use different TCR and demonstrate two distinct serotype specificities recognize the same 9-mer peptide in the context of HLA-B35...
  67. ncbi Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones
    S Green
    Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA
    Virology 234:383-6. 1997
    ..These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes...
  68. doi Characterization of Asn130-to-Ala mutant of dengue type 1 virus NS1 protein
    Shigeru Tajima
    Laboratory of Vector Borne Viruses, Department of Virology 1, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
    Virus Genes 36:323-9. 2008
    ..Our results suggest that Asn130 of the DENV-1 NS1 is important for viral replication in both mammalian and mosquito cells...
  69. ncbi Immunoaffinity purification of native dimer forms of the flavivirus non-structural glycoprotein, NS1
    A K Falconar
    Sir Albert Sakzewski Virus Research Laboratory, Royal Children s Hospital, Brisbane, Australia
    J Virol Methods 30:323-32. 1990
    ..In moderately sized virus growth experiments, greater than 1 mg quantities of purified NS1 were obtained...
  70. doi Nuclear localization of dengue virus capsid protein is required for DAXX interaction and apoptosis
    Janjuree Netsawang
    Medical Molecular Biology Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Virus Res 147:275-83. 2010
    ..This work is the first to demonstrate that nuclear localization of DENV C is required for DAXX interaction and apoptosis...
  71. ncbi Nuclear localization of dengue virus nonstructural protein 5 through its importin alpha/beta-recognized nuclear localization sequences is integral to viral infection
    Melinda J Pryor
    Department of Biochemistry and Molecular Biology, Monash University, Monash, Victoria 3800, Australia
    Traffic 8:795-807. 2007
    ..The results overall indicate that NS5 nuclear localization through the aNLS is integral to viral infection, with significant implications for other flaviviruses of medical importance, such as yellow fever and West Nile viruses...
  72. ncbi Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles
    R J Sugrue
    Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore
    J Gen Virol 78:1861-6. 1997
    ..This is the first report that expression of the structural genes of a flavivirus in yeast is able to generate particulate structures that resemble virions...
  73. pmc Solution structure of the envelope protein domain III of dengue-4 virus
    David E Volk
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 1157, USA
    Virology 364:147-54. 2007
    ..Important structural differences between DEN4-rED3 and ED3 domains of DEN2, DEN3 and other flaviviruses are discussed...
  74. ncbi Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells
    B Puri
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
    J Gen Virol 78:2287-91. 1997
    ..Important nucleotide base changes responsible for attenuation accumulated between 45AZ5 PDK-O and 27...
  75. ncbi Modulation of the nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase
    Changsuek Yon
    Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington D C 20057, USA
    J Biol Chem 280:27412-9. 2005
    ..Moreover, the conserved motif, 184RKRK, of NS3 played a crucial role in binding to RNA substrate and modulating the NTPase/RNA helicase and RTPase activities of NS3...
  76. ncbi Production of anti-dengue NS1 monoclonal antibodies by DNA immunization
    Chunya Puttikhunt
    National Center for Genetic Engineering and Biotechnology BIOTEC, National Science and Technology Development Agency, Bangkok 10700, Thailand
    J Virol Methods 109:55-61. 2003
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  77. ncbi Chimeric flaviviruses: novel vaccines against dengue fever, tick-borne encephalitis, and Japanese encephalitis
    CHING JUH LAI
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 61:469-509. 2003
    ..The chimeric flavivirus strategy has led to the rapid development of novel live-attenuated vaccines against dengue, TBE, JE, and West Nile viruses...
  78. ncbi Heparan sulfate-mediated binding of infectious dengue virus type 2 and yellow fever virus
    Raphaele Germi
    Laboratoire de Virologie Moléculaire et Structurale EA 2939, Universite Joseph Fourier, Grenoble, La Tronche, 38700, France
    Virology 292:162-8. 2002
    ..Heparin also inhibited Yellow fever virus infection by 97%. Using infectious virus, the present work shows the importance of heparan sulfate in binding and infection of these two flaviviruses...
  79. pmc Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function
    B D Lindenbach
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    J Virol 73:4611-21. 1999
    ..These findings suggest a potential mechanism for integrating NS1 into the cytoplasmic process of RNA replication...
  80. pmc PrM- and cell-binding domains of the dengue virus E protein
    S Wang
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Virol 73:2547-51. 1999
    ....
  81. pmc Solution structure of dengue virus capsid protein reveals another fold
    Lixin Ma
    Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 101:3414-9. 2004
    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
  82. pmc Switching the substrate specificity of the two-component NS2B-NS3 flavivirus proteinase by structure-based mutagenesis
    Sergey A Shiryaev
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, USA
    J Virol 81:4501-9. 2007
    ....
  83. pmc The secreted form of dengue virus nonstructural protein NS1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity
    Sophie Alcon-LePoder
    U P Interactions Moléculaires Flavivirus Hôtes, Institut Pasteur, Paris, France
    J Virol 79:11403-11. 2005
    ....

Research Grants3

  1. Cellular Immune Response to the SARS Human Coronavirus
    DANIEL LIBRATY; Fiscal Year: 2005
    ..Such studies will assess potential correlates of protective adaptive immunity to SARS HuCoV infection. ..
  2. A Study of Protective Immunity Against Dengue in Infants
    DANIEL LIBRATY; Fiscal Year: 2007
    ..The research will generate vital data for the effective testing of dengue vaccines and future public health vaccination campaigns throughout the region. ..