DENV_gp1

Summary

Gene Symbol: DENV_gp1
Description: Polyprotein
Species: Dengue virus 2

Top Publications

  1. ncbi Mutational analysis of a neutralization epitope on the dengue type 2 virus (DEN2) envelope protein: monoclonal antibody resistant DEN2/DEN4 chimeras exhibit reduced mouse neurovirulence
    K Hiramatsu
    Molecular Viral Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Virology 224:437-45. 1996
  2. ncbi A single nucleotide change in the E protein gene of dengue virus 2 Mexican strain affects neurovirulence in mice
    I J Sánchez
    Departamento de Biologia Molecular, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico
    J Gen Virol 77:2541-5. 1996
  3. ncbi Analysis of antibody-independent binding of dengue viruses and dengue virus envelope protein to human myelomonocytic cells and B lymphocytes
    H Bielefeldt-Ohmann
    Department of Microbiology, University of Queensland, Brisbane, Australia
    Virus Res 57:63-79. 1998
  4. pmc A ligand-binding pocket in the dengue virus envelope glycoprotein
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6986-91. 2003
  5. ncbi Growth and production of the dengue virus in C6/36 cells and identification of a laminin-binding protein as a candidate serotype 3 and 4 receptor protein
    Prirayapak Sakoonwatanyoo
    Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Bangkok, Thailand
    Intervirology 49:161-72. 2006
  6. pmc DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells
    Boonrat Tassaneetrithep
    Division of Retrovirology, Walter Reed Army Institite of Research and Henry M Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA
    J Exp Med 197:823-9. 2003
  7. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
  8. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
  9. pmc West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H
    Kyung Min Chung
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 103:19111-6. 2006
  10. ncbi Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1
    J J Schlesinger
    J Gen Virol 68:853-7. 1987

Research Grants

Scientific Experts

Detail Information

Publications84

  1. ncbi Mutational analysis of a neutralization epitope on the dengue type 2 virus (DEN2) envelope protein: monoclonal antibody resistant DEN2/DEN4 chimeras exhibit reduced mouse neurovirulence
    K Hiramatsu
    Molecular Viral Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Virology 224:437-45. 1996
    ....
  2. ncbi A single nucleotide change in the E protein gene of dengue virus 2 Mexican strain affects neurovirulence in mice
    I J Sánchez
    Departamento de Biologia Molecular, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico
    J Gen Virol 77:2541-5. 1996
    ..This substitution falls in domain III of the E protein, which plays an important role in viral binding; therefore, we propose that the substitution affects virulence and cellular tropism...
  3. ncbi Analysis of antibody-independent binding of dengue viruses and dengue virus envelope protein to human myelomonocytic cells and B lymphocytes
    H Bielefeldt-Ohmann
    Department of Microbiology, University of Queensland, Brisbane, Australia
    Virus Res 57:63-79. 1998
    ....
  4. pmc A ligand-binding pocket in the dengue virus envelope glycoprotein
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6986-91. 2003
    ..These features point to a structural pathway for the fusion-activating transition and suggest a strategy for finding small-molecule inhibitors of dengue and other flaviviruses...
  5. ncbi Growth and production of the dengue virus in C6/36 cells and identification of a laminin-binding protein as a candidate serotype 3 and 4 receptor protein
    Prirayapak Sakoonwatanyoo
    Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Bangkok, Thailand
    Intervirology 49:161-72. 2006
    ....
  6. pmc DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells
    Boonrat Tassaneetrithep
    Division of Retrovirology, Walter Reed Army Institite of Research and Henry M Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA
    J Exp Med 197:823-9. 2003
    ..Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection...
  7. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell...
  8. ncbi A structural perspective of the flavivirus life cycle
    Suchetana Mukhopadhyay
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, Indiana 47907 2054, USA
    Nat Rev Microbiol 3:13-22. 2005
    ..This review focuses mainly on structural studies of dengue virus...
  9. pmc West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H
    Kyung Min Chung
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 103:19111-6. 2006
    ..Accordingly, extracellular NS1 may function to minimize immune system targeting of West Nile virus by decreasing complement recognition of infected cells...
  10. ncbi Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1
    J J Schlesinger
    J Gen Virol 68:853-7. 1987
    ..Induction of a protective immune response by NS1 suggests that it be considered for incorporation into possible synthetic or recombinant DNA DEN vaccines...
  11. ncbi Analysis of the steps involved in Dengue virus entry into host cells
    S L Hung
    Institute of Oral Biology, National Yang Ming University, Taipei, Taiwan
    Virology 257:156-67. 1999
    ..This suggests that cell surface heparan sulfate functions in both viral attachment and penetration of DEN-2 virus. In conclusion, our results further elucidated some aspects of the dengue virus entry process...
  12. ncbi Visualization of membrane protein domains by cryo-electron microscopy of dengue virus
    Wei Zhang
    Department of Biological Sciences, Lilly Hall, 915 W State Street, Purdue University, West Lafayette, Indiana 47907, USA
    Nat Struct Biol 10:907-12. 2003
    ..This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus...
  13. ncbi Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53
    R M Kinney
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA
    Virology 230:300-8. 1997
    ..Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice...
  14. pmc PrM- and cell-binding domains of the dengue virus E protein
    S Wang
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Virol 73:2547-51. 1999
    ....
  15. ncbi High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever
    Daniel H Libraty
    Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655, USA
    J Infect Dis 186:1165-8. 2002
    ..An elevated free sNS1 level (> or =600 ng/mL) within 72 h of illness onset identified patients at risk for developing DHF...
  16. ncbi Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication
    J M MacKenzie
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Virology 220:232-40. 1996
    ..These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication...
  17. pmc The secreted form of dengue virus nonstructural protein NS1 is endocytosed by hepatocytes and accumulates in late endosomes: implications for viral infectivity
    Sophie Alcon-LePoder
    U P Interactions Moléculaires Flavivirus Hôtes, Institut Pasteur, Paris, France
    J Virol 79:11403-11. 2005
    ....
  18. ncbi Structure of the dengue virus envelope protein after membrane fusion
    Yorgo Modis
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:313-9. 2004
    ..Specific features of the folded-back structure suggest strategies for inhibiting flavivirus entry...
  19. ncbi Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus
    E G Westaway
    Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Virology 234:31-41. 1997
    ..Dual IF with antibodies to double-stranded RNA showed that cytoplasmic locations of C and NS4B were apparently associated in part with the sites of viral RNA synthesis which were resistant to solubilization by Triton X-100...
  20. pmc An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization
    Marie Pierre Egloff
    Architecture et Fonction des Macromolecules Biologiques, UMR 6098 CNRS et Université Aix Marseille I et II, ESIL, Campus de Luminy, F 13288 Marseille Cedex 09, France
    EMBO J 21:2757-68. 2002
    ..As RNA capping is an essential viral function, these results provide a structural basis for the rational design of drugs against the emerging flaviviruses...
  21. doi Structure of the immature dengue virus at low pH primes proteolytic maturation
    I Mei Yu
    Department of Biological Sciences, 915 West State Street, Purdue University, West Lafayette, IN 47907 2054, USA
    Science 319:1834-7. 2008
    ..These results suggest a mechanism by which flaviviruses are processed and stabilized in the host cell secretory pathway...
  22. pmc Crystal structure of west nile virus envelope glycoprotein reveals viral surface epitopes
    Ryuta Kanai
    266Department of Molecular Biophysics and Biochemistry, The Bass Center for Structural Biology, Yale University, 266 Whitney Ave, New Haven, Connecticut 06520, USA
    J Virol 80:11000-8. 2006
    ..By revealing the details of the molecular landscape of the West Nile virus surface, our structure will assist the design of antiviral vaccines and therapeutics...
  23. ncbi Production of anti-dengue NS1 monoclonal antibodies by DNA immunization
    Chunya Puttikhunt
    National Center for Genetic Engineering and Biotechnology BIOTEC, National Science and Technology Development Agency, Bangkok 10700, Thailand
    J Virol Methods 109:55-61. 2003
    ..This has opened up a possibility of producing monoclonal antibodies to rare viral proteins that are difficult to isolate or purify...
  24. ncbi Identification of an epitope on the dengue virus membrane (M) protein defined by cross-protective monoclonal antibodies: design of an improved epitope sequence based on common determinants present in both envelope (E and M) proteins
    A K Falconar
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, U K
    Arch Virol 144:2313-30. 1999
    ..These peptide sequence will now be tested for their ability to generate cross-protective antibodies against each dengue virus serotype when delivered with appropriate T-helper epitopes...
  25. ncbi Histidine 39 in the dengue virus type 2 M protein has an important role in virus assembly
    Melinda J Pryor
    Department of Microbiology, Monash University, Clayton 3168, Victoria, Australia
    J Gen Virol 85:3627-36. 2004
    ..The results of this study pinpoint a critical residue in the M protein that potentially plays a role in viral morphogenesis, secretion and entry...
  26. pmc The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells
    Ricardo F Mercado-Curiel
    Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Ave Instituto Politécnico Nacional 2508 Col San Pedro Zacatenco, C P 07360, Mexico, D F, Mexico
    BMC Microbiol 6:85. 2006
    ..Determination of which of them serve as bona fide receptors for this virus in the vector may be relevant to treating DENV infection and in designing control strategies...
  27. doi Domain-III FG loop of the dengue virus type 2 envelope protein is important for infection of mammalian cells and Aedes aegypti mosquitoes
    Steven M Erb
    Arthropod Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virology 406:328-35. 2010
    ..Reduced binding of DENV2 type-specific monoclonal antibody 3H5 to mutant viruses confirmed the FG loop motif as its target epitope...
  28. pmc Dengue virus structural differences that correlate with pathogenesis
    K C Leitmeyer
    Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227 5301, USA
    J Virol 73:4738-47. 1999
    ..This new approach to the study of dengue virus genome differences should better reflect the true composition of viral RNA populations in the natural host and permit their association with pathogenesis...
  29. pmc Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles
    Jiraphan Junjhon
    Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
    J Virol 84:8353-8. 2010
    ..These findings are consistent with a model suggesting the progressive mode of prM cleavage...
  30. ncbi Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement
    Panisadee Avirutnan
    Medical Molecular Biology Unit, Siriraj Hospital, Mahidol University, Bangkok, Thailand
    J Infect Dis 193:1078-88. 2006
    ..Thirty years ago, complement activation was proposed to be a key underlying event, but the cause of complement activation has remained unknown...
  31. pmc The length of and nonhydrophobic residues in the transmembrane domain of dengue virus envelope protein are critical for its retention and assembly in the endoplasmic reticulum
    Szu Chia Hsieh
    Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    J Virol 84:4782-97. 2010
    ....
  32. ncbi E/NS1 modifications of dengue 2 virus after serial passages in mammalian and/or mosquito cells
    Wei June Chen
    Department of Public Health and Parasitology, Chang Gung University, Tao Yuan, Taiwan
    Intervirology 46:289-95. 2003
    ..The number of dengue virus lineages has been increasing over time. The aim of this study was to identify the genetic diversity of dengue 2 virus serially transferred in mammalian and/or mosquito cells...
  33. pmc Nuclear localization of flavivirus RNA synthesis in infected cells
    Pradeep Devappa Uchil
    Department of Microbiology and Cell Biology, Room 254A, Sir C V Raman Avenue, Indian Institute of Science, Bangalore 560012, India
    J Virol 80:5451-64. 2006
    ..Thus, we conclusively demonstrate for the first time that the host cell nucleus functions as an additional site for the presence of functionally active flaviviral replicase complex...
  34. doi Anti-dengue virus nonstructural protein 1 antibodies recognize protein disulfide isomerase on platelets and inhibit platelet aggregation
    Hsien Jen Cheng
    Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan, Taiwan
    Mol Immunol 47:398-406. 2009
    ..In conclusion, anti-DV NS1 antibodies cross-react with PDI on platelet surface causing inhibition of platelet aggregation, which may provide implications in dengue disease pathogenesis...
  35. ncbi A structural basis for the inhibition of the NS5 dengue virus mRNA 2'-O-methyltransferase domain by ribavirin 5'-triphosphate
    Delphine Benarroch
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieur d Ingénieurs de Luminy Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    J Biol Chem 279:35638-43. 2004
    ....
  36. ncbi Antiviral action of nitric oxide on dengue virus type 2 replication
    Ratree Takhampunya
    Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand
    J Gen Virol 87:3003-11. 2006
    ..These results suggest that the inhibitory effect of NO on DENV infection is partly via inhibition of the RdRp activity, which then downregulates viral RNA synthesis...
  37. ncbi Dengue virus nonstructural protein 1 is expressed in a glycosyl-phosphatidylinositol-linked form that is capable of signal transduction
    M G Jacobs
    Department of Paediatrics, Imperial College School of Medicine, Norfolk Place, London W2 1PG, U K
    FASEB J 14:1603-10. 2000
    ..Signal transduction by a GPI-anchored viral antigen interacting with a specific antibody that it induces is a new concept in the pathogenesis of viral disease...
  38. pmc Mechanism of NS2B-mediated activation of NS3pro in dengue virus: molecular dynamics simulations and bioassays
    Zhili Zuo
    Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore
    J Virol 83:1060-70. 2009
    ....
  39. ncbi Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries
    Jun Li
    Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
    J Biol Chem 280:28766-74. 2005
    ..The specificities observed for dengue NS2B/NS3 have features in common with the physiological cleavage sites in the dengue polyprotein; however, all sites reveal previously unrecognized suboptimal sequences...
  40. pmc Flavivirus activates phosphatidylinositol 3-kinase signaling to block caspase-dependent apoptotic cell death at the early stage of virus infection
    Chyan Jang Lee
    Graduate Institute of Life Sciences, Taipei, Taiwan, Republic of China
    J Virol 79:8388-99. 2005
    ..Collectively, our results suggest an anti-apoptotic role for the PI3K/Akt pathway triggered by JEV and DEN-2 to protect infected cells from early apoptotic cell death...
  41. pmc Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase
    Sergey A Shiryaev
    The Burnham Institute, La Jolla, CA 92037, USA
    Biochem J 393:503-11. 2006
    ..Overall, our findings represent a foundation for in-depth mechanistic and structural studies as well as for the design of novel and efficient inhibitors of WNV NS3...
  42. ncbi Development of novel antivirals against flaviviruses
    Chinmay G Patkar
    Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907 2054, USA
    Novartis Found Symp 277:41-52; discussion 52-6, 71-3, 251-3. 2006
    ....
  43. pmc Probing the substrate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides
    Pornwaratt Niyomrattanakit
    Laboratory of Molecular Virology, Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Phutthamonthon 4 Rd, Nakornpathom 73170, Thailand
    Biochem J 397:203-11. 2006
    ....
  44. ncbi Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus
    Paul Erbel
    Novartis Institutes for BioMedical Research, Protease Platform, 4002 Basel, Switzerland
    Nat Struct Mol Biol 13:372-3. 2006
    ..These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation...
  45. pmc A 5' RNA element promotes dengue virus RNA synthesis on a circular genome
    Claudia V Filomatori
    Fundacion Instituto Leloir, Buenos Aires C1405BWE, Argentina
    Genes Dev 20:2238-49. 2006
    ..These findings provide an explanation for the strict requirement of dengue virus genome cyclization during viral replication...
  46. ncbi Blocking the dengue virus 2 infections on BHK-21 cells with purified recombinant dengue virus 2 E protein expressed in Escherichia coli
    Mei Wui Chiu
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, ROC
    Biochem Biophys Res Commun 309:672-8. 2003
    ....
  47. ncbi Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica
    J T Roehrig
    Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
    Virology 246:317-28. 1998
    ..Domains A and B were spatially associated. As with tick-borne encephalitis virus, determination of domain C was more problematic; however, at least four epitopes had biochemical characteristics consistent with C-domain epitopes...
  48. ncbi Conversion of dengue virus replicative form RNA (RF) to replicative intermediate (RI) by nonstructural proteins NS-5 and NS-3
    K Raviprakash
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889, USA
    Am J Trop Med Hyg 58:90-5. 1998
    ..The recombinant proteins, however, required one or more cellular protein(s) for their activity. Examination of NS-3 protein sequence revealed heretofore unnoticed sequence similarities with other polymerases...
  49. pmc Dengue virus protein recognition by virus-specific murine CD8+ cytotoxic T lymphocytes
    A L Rothman
    Department of Medicine, University of Massachusetts Medical Center, Worcester 01655
    J Virol 67:801-6. 1993
    ..Since human dengue virus-specific CTL show similar patterns of recognition, these findings suggest that nonstructural proteins should be considered in designing vaccines against dengue...
  50. ncbi Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5
    M Kapoor
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160, USA
    J Biol Chem 270:19100-6. 1995
    ....
  51. pmc Study of Dengue virus infection in SCID mice engrafted with human K562 cells
    Y L Lin
    Institute of Biomedical Sciences, Academia Sinica, National Taiwan University, Taipei, Taiwan, Republic of China
    J Virol 72:9729-37. 1998
    ..The system described herein offers an opportunity to explore DEN replication in vivo and to test various antiviral protocols in infected hosts...
  52. ncbi Dengue virus-specific, HLA-B35-restricted, human CD8+ cytotoxic T lymphocyte (CTL) clones. Recognition of NS3 amino acids 500 to 508 by CTL clones of two different serotype specificities
    P G Livingston
    Department of Medicine, University of Massachusetts Medical Center, Worcester 01655
    J Immunol 154:1287-95. 1995
    ..These results indicate that CD8+ CTL clones that use different TCR and demonstrate two distinct serotype specificities recognize the same 9-mer peptide in the context of HLA-B35...
  53. ncbi Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses
    J F Bazan
    Department of Biophysics, University of California, Berkeley 94720
    Virology 171:637-9. 1989
    ..Structural modeling of the putative flaviviral protease domain suggests amino acids that are crucial for catalytic activity and substrate binding...
  54. pmc N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases
    A E Gorbalenya
    Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region
    Nucleic Acids Res 17:3889-97. 1989
    ..The protease domain is probably involved in the processing of viral non-structural proteins...
  55. ncbi Topological mapping of unique epitopes on the dengue-2 virus NS1 protein using monoclonal antibodies
    E A Henchal
    J Gen Virol 68:845-51. 1987
    ..Four preparations had detectable complement fixation titres using Thai DEN-2 virus antigen. Six spatially unique epitopes were identified using competitive binding assays...
  56. pmc In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3
    F Preugschat
    Division of Biology, California Institute of Technology, Pasadena 91125
    J Virol 64:4364-74. 1990
    ..The data from these expression experiments confirm that NS3 is the viral proteinase responsible for cleavage events generating the amino termini of NS2B and NS3 and presumably for cleavages generating the termini of NS4A and NS5 as well...
  57. ncbi Molecular evolution and distribution of dengue viruses type 1 and 2 in nature
    R Rico-Hesse
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510
    Virology 174:479-93. 1990
    ..This is the first genetic evidence of the existence of a sylvatic cycle of dengue virus, which is clearly distinct from outbreak viruses...
  58. ncbi Processing of nonstructural proteins NS4A and NS4B of dengue 2 virus in vitro and in vivo
    F Preugschat
    Division of Biology, California Institute of Technology, Pasadena 91125
    Virology 185:689-97. 1991
    ....
  59. pmc The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids
    H Li
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
    J Virol 73:3108-16. 1999
    ....
  60. ncbi Homology model of the dengue 2 virus NS3 protease: putative interactions with both substrate and NS2B cofactor
    R I Brinkworth
    Centre for Drug Design and Development, University of Queensland, St Lucia, Brisbane, Australia
    J Gen Virol 80:1167-77. 1999
    ....
  61. ncbi Enhancing biosynthesis and secretion of premembrane and envelope proteins by the chimeric plasmid of dengue virus type 2 and Japanese encephalitis virus
    Gwong Jen J Chang
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Service, Post Office Box 2087, Fort Collins, CO 80522, USA
    Virology 306:170-80. 2003
    ..Eleven of twelve 2-day-old neonatal mice, derived from a pCB8D2-2J-2-9-1 immunized female mouse, survived intraperitoneal challenge of DEN-2 New Guinea C virus...
  62. pmc Infection of human cells by dengue virus is modulated by different cell types and viral strains
    M S Diamond
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA
    J Virol 74:7814-23. 2000
    ..Thus, depending on the cell type and viral isolate, blocks that limit the production of infectious virus in vitro may occur at distinct steps in the pathway of cellular infection...
  63. ncbi Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro
    R Yusof
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Biol Chem 275:9963-9. 2000
    ..These results suggest that heterodimerization of the NS3pro domain with NS2B generates additional specific interactions with the P2 and P3 residues of the substrates...
  64. ncbi Vaccination of rhesus macaques against dengue-2 virus with a plasmid DNA vaccine encoding the viral pre-membrane and envelope genes
    Robert Putnak
    Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910 7500, USA
    Am J Trop Med Hyg 68:469-76. 2003
    ..Long-term protection following DNA vaccination may require revaccination, higher doses of DNA, or a vaccine that contains additional epitopes or adjuvants...
  65. ncbi Requirements for West Nile virus (-)- and (+)-strand subgenomic RNA synthesis in vitro by the viral RNA-dependent RNA polymerase expressed in Escherichia coli
    Masako Nomaguchi
    Department of Microbiology and Immunology, Georgetown University Medical Center, SW309 Med Dent Building, 3900 Reservoir Road, Washington, D C 20057, USA
    J Biol Chem 279:12141-51. 2004
    ..These results support a model that "cyclization" of the viral RNA play a role for (-)-strand RNA synthesis but not for (+)-strand RNA synthesis...
  66. ncbi Antigenic structure of flavivirus proteins
    John T Roehrig
    Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
    Adv Virus Res 59:141-75. 2003
    ..To prepare effective and inexpensive subunit vaccines, we will need to continue to better understand these structure-function relationships of flavivirus proteins...
  67. pmc Antagonism of the complement component C4 by flavivirus nonstructural protein NS1
    Panisadee Avirutnan
    Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Exp Med 207:793-806. 2010
    ..Through this mechanism, NS1 protects DENV from complement-dependent neutralization in solution. These studies define a novel immune evasion mechanism for restricting complement control of microbial infection...
  68. ncbi Chimeric flaviviruses: novel vaccines against dengue fever, tick-borne encephalitis, and Japanese encephalitis
    CHING JUH LAI
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Adv Virus Res 61:469-509. 2003
    ..The chimeric flavivirus strategy has led to the rapid development of novel live-attenuated vaccines against dengue, TBE, JE, and West Nile viruses...
  69. pmc Differential modulation of prM cleavage, extracellular particle distribution, and virus infectivity by conserved residues at nonfurin consensus positions of the dengue virus pr-M junction
    Jiraphan Junjhon
    Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
    J Virol 82:10776-91. 2008
    ....
  70. ncbi Antiplatelet autoantibodies elicited by dengue virus non-structural protein 1 cause thrombocytopenia and mortality in mice
    D S Sun
    Institute of Molecular and Cellular Biology, Tzu Chi University, Hualien, Taiwan
    J Thromb Haemost 5:2291-9. 2007
    ..The mechanisms responsible for thrombocytopenia associated with dengue fever (DF) and dengue hemorrhage fever (DHF) remain unclear...
  71. ncbi Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease
    Aloke K Bera
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:12883-92. 2007
    ..Formation of an active protease was found to require neither cleavage of NS2B from NS3 nor a free NS3 N terminus. Evidence was also obtained for product inhibition of the protease by the cleaved C terminus of NS2B...
  72. pmc Solution structure of dengue virus capsid protein reveals another fold
    Lixin Ma
    Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA
    Proc Natl Acad Sci U S A 101:3414-9. 2004
    ..We propose a model for the interaction of dengue C protein with RNA and the viral membrane that is based on the asymmetric charge distribution of the protein and is consistent with previously reported results...
  73. ncbi Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer
    G Winkler
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854
    Virology 162:187-96. 1988
    ..It is formed between 20 and 40 min after NS1 is synthesized and before the protein passes the Golgi apparatus. Both soluble and pelletable extracellular NS1 are also found as the high-molecular-weight form...
  74. ncbi Molecular biology of flaviviruses
    Eva Harris
    Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, CA 94720 7360, USA
    Novartis Found Symp 277:23-39; discussion 40, 71-3, 251-3. 2006
    ..Although much remains to be elucidated about the molecular biology of flavivirus infection, progress is being made towards defining the cis and trans factors that regulate flavivirus translation and replication...
  75. ncbi Synthesis and characterization of an infectious dengue virus type-2 RNA genome (New Guinea C strain)
    M Kapoor
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66103, USA
    Gene 162:175-80. 1995
    ..The full-length infectious clone for DEN-2 should be useful for the study of molecular mechanisms involved in viral RNA replication and virus assembly...
  76. ncbi Crystallization, characterization and measurement of MAD data on crystals of dengue virus NS3 serine protease complexed with mung-bean Bowman-Birk inhibitor
    H M Krishna Murthy
    Center for Macromolecular Crystallography, University of Alabama at Birmingham, 79 THT, MCLM 244, 1918 University Boulevard, Birmingham, AL 35294 0005, USA
    Acta Crystallogr D Biol Crystallogr 55:1370-2. 1999
    ..The structure, when determined, will provide a structural basis for the design, synthesis and evaluation of inhibitors of the protease for chemotherapy of dengue infections...
  77. pmc An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients
    P R Young
    Sir Albert Sakzewski Virus Research Centre, The Royal Children s Hospital, Herston, Brisbane 4029, Australia
    J Clin Microbiol 38:1053-7. 2000
    ....
  78. ncbi Dengue virus type 2 antagonizes IFN-alpha but not IFN-gamma antiviral effect via down-regulating Tyk2-STAT signaling in the human dendritic cell
    Ling Jun Ho
    Division of Gerontology Research, National Health Research Institute, Academia Sinica, Taipei, Taiwan
    J Immunol 174:8163-72. 2005
    ..These observations provide very useful messages with physiological significance in investigation of the pathogenesis, the defense mechanisms of human hosts and the therapeutic considerations in DV infection...
  79. ncbi Structural and functional analysis of dengue virus RNA
    Diego E Alvarez
    Fundacion Instituto Leloir, Patricias Argentinas 435, Buenos Aires, Argentina
    Novartis Found Symp 277:120-32; discussion 132-5, 251-3. 2006
    ..Furthermore, a systematic mutational analysis of UAR sequences indicated that, beside the role in RNA cyclization, specific nucleotides within UAR are also important for efficient RNA synthesis...
  80. ncbi Catalytically active Dengue virus NS3 protease forms aggregates that are separable by size exclusion chromatography
    Tracy L Arakaki
    Centre for Drug Design and Development, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Protein Expr Purif 25:241-7. 2002
    ..The ultimate goal of these studies is to obtain a crystal structure of a catalytically active form of the Dengue virus NS3 protease for structure-based drug design...
  81. ncbi The type 2 dengue virus envelope protein interacts with small ubiquitin-like modifier-1 (SUMO-1) conjugating enzyme 9 (Ubc9)
    Mei Wui Chiu
    Department of Biological Science and Technology, National Chiao Tung University, 75 Po Ai Street, Hsinchu, Taiwan, ROC
    J Biomed Sci 14:429-44. 2007
    ..This is the first report that DV envelope proteins can interact with the protein of sumoylation system and Ubc9 may involve in the host defense system to prevent virus propagation...
  82. pmc Switching the substrate specificity of the two-component NS2B-NS3 flavivirus proteinase by structure-based mutagenesis
    Sergey A Shiryaev
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA, USA
    J Virol 81:4501-9. 2007
    ....
  83. pmc Evidence that flavivirus NS1-NS2A cleavage is mediated by a membrane-bound host protease in the endoplasmic reticulum
    B Falgout
    Laboratory of Infectious Disease, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA
    J Virol 69:7232-43. 1995
    ....
  84. ncbi De novo synthesis of RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase
    M Ackermann
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, KS 66160 7421, USA
    J Biol Chem 276:39926-37. 2001
    ..A model is proposed that a specific conformation of the viral polymerase and/or structure at the 3' end of the template RNA is required for de novo initiation...

Research Grants3

  1. Cellular Immune Response to the SARS Human Coronavirus
    DANIEL LIBRATY; Fiscal Year: 2005
    ..Such studies will assess potential correlates of protective adaptive immunity to SARS HuCoV infection. ..
  2. A Study of Protective Immunity Against Dengue in Infants
    DANIEL LIBRATY; Fiscal Year: 2007
    ..The research will generate vital data for the effective testing of dengue vaccines and future public health vaccination campaigns throughout the region. ..