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Genomes and Genes | degSSummaryGene Symbol: degS Description: serine endoprotease, periplasmic Alias: ECK3224, JW3204, hhoB, htrH Species: Top Publications
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Cloning and sequence of the mdh structural gene of Escherichia coli coding for malate dehydrogenaseR F Vogel
Physiologisch Chemisches Institut im Medizinisch Naturwissenschaftlichen Forschungszentrum der Universität Tübingen, Federal Republic of Germany
Arch Microbiol 149:36-42. 1987..The structures for transcription initiation and termination were similar to those previously described for E. coli. Amino acid sequence homologies between pro- and eucaryotic malate dehydrogenases are discussed...- Fine-tuning of the Escherichia coli sigmaE envelope stress response relies on multiple mechanisms to inhibit signal-independent proteolysis of the transmembrane anti-sigma factor, RseAIrina L Grigorova
Graduate Group in Biophysics, University of California, San Francisco, San Francisco, California 94143, USA
Genes Dev 18:2686-97. 2004..Upon activation by OMP C termini, the membrane localized DegS protease cleaves RseA in its periplasmic domain, the membrane-embedded protease RseP (YaeL) cleaves RseA near the .. - Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coliVéronique Douchin
Signalisation et Réseaux de Régulations Bactériens, Institut de Genetique et Microbiologie, CNRS UMR8621 IFR115, Centre Scientifique d Orsay, Universite Paris Sud, 91405 Orsay Cedex, France
J Biol Chem 281:12253-9. 2006..SigmaE is released in response to stress through the successive RseA cleavage by DegS and the RIP protease RseP. SigmaE and proteases that free it from RseA are essential... - Outer membrane vesicle production by Escherichia coli is independent of membrane instabilityAmanda J McBroom
Department of Biochemistry, Duke University Medical Center, Box 3711, Durham, NC 27710, USA
J Bacteriol 188:5385-92. 2006..Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesiculation levels, demonstrating that vesicle production is not predictive of envelope instability... - Inhibition of regulated proteolysis by RseBBrent O Cezairliyan
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139
Proc Natl Acad Sci U S A 104:3771-6. 2007..This response is initiated by DegS cleavage of the periplasmic domain of RseA, a transmembrane protein... - [Characterization of the HtrA family of proteins]Dorota Zurawa-Janicka
Katedra Biochemii, Instytut Biologii, Uniwersytet Gdanski, Gdansk
Postepy Biochem 53:27-36. 2007..This article summarizes recent studies regarding the HtrA family of proteins, their structure, regulation and function. It also presents practical applications of this knowledge and perspective of its use in the future... - Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stressHanna Hasselblatt
Research Institute of Molecular Pathology IMP, A 1030 Vienna, Austria
Genes Dev 21:2659-70. 2007..The PDZ-protease DegS recognizes these mislocalized OMPs and initiates a proteolytic cascade that ultimately leads to the sigmaE-driven .. - Allosteric activation of DegS, a stress sensor PDZ proteaseJungsan Sohn
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 131:572-83. 2007..coli, the C termini of outer-membrane proteins (OMPs) bind to the PDZ domains of the trimeric DegS protease and activate cleavage of RseA, a transmembrane transcriptional regulator... - Characterization of the autocleavage process of the Escherichia coli HtrA protein: implications for its physiological roleAhmad Jomaa
Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N3Z5, Canada
J Bacteriol 191:1924-32. 2009..Our data support a model in which the physiological role of the self-cleavage process is to eliminate the excess of HtrA once the stress conditions cease... - OMP peptides modulate the activity of DegS protease by differential binding to active and inactive conformationsJungsan Sohn
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Mol Cell 33:64-74. 2009Upon sensing misfolded outer-membrane porins (OMPs) in the periplasm, the E. coli DegS protease cleaves RseA, a transmembrane regulator, transmitting a signal to activate cytoplasmic gene expression... - Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coliMichael Meltzer
Centre for Medical Biotechnology, FB Biology and Geography, University Duisburg Essen, Unistr 2, 45117 Essen, Germany
Res Microbiol 160:660-6. 2009Two members of the widely conserved HtrA family of serine proteases, DegP and DegS, are key players in extracytoplasmic protein quality control... - Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavageXiaochun Li
Ministry of Education Protein Science Laboratory and Center for Structural Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
Proc Natl Acad Sci U S A 106:14837-42. 2009..mechanism, we reconstituted sequential, in vitro cleavages of the Escherichia coli transmembrane protein RseA by DegS (S1P) and RseP (S2P)... - OMP peptides activate the DegS stress-sensor protease by a relief of inhibition mechanismJungsan Sohn
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Structure 17:1411-21. 2009..periplasm, C-terminal peptides of misfolded outer-membrane porins (OMPs) bind to the PDZ domains of the trimeric DegS protease, triggering cleavage of a transmembrane regulator and transcriptional activation of stress genes... - Conversion of a regulatory into a degradative proteaseSonja Hasenbein
Centre for Medical Biotechnology, FB Biology and Geography, University Duisburg Essen, 45117 Essen, Germany
J Mol Biol 397:957-66. 2010The PDZ protease DegS senses mislocalized outer membrane proteins and initiates the sigmaE pathway in the bacterial periplasm... - Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradationJulia M Flynn
Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genes Dev 18:2292-301. 2004..Thus, these experiments elucidate the final steps in induction of the extracytoplasmic stress response and reveal that SspB delivers a broader spectrum of substrates to ClpXP than has been recognized... - Expression, crystallization and crystallographic analysis of DegS, a stress sensor of the bacterial periplasmMartin Grininger
Max Planck Institute for Biochemistry, Department of Membrane Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
Acta Crystallogr D Biol Crystallogr 60:1429-31. 2004..In Escherichia coli, a protein DegS has been identified as being a periplasmic stress sensor for unfolded or misfolded outer membrane proteins (OMPs)... - Structural analysis of DegS, a stress sensor of the bacterial periplasmKornelius Zeth
Department of Membrane Biochemistry, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
FEBS Lett 569:351-8. 2004..In Escherichia coli the membrane-bound protease DegS has been identified as the periplasmic stress sensor for unfolded outer membrane proteins (OMPs)... - Characterization of degQ and degS, Escherichia coli genes encoding homologs of the DegP proteaseP R Waller
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
J Bacteriol 178:1146-53. 1996The degQ and degS genes of Escherichia coli encode proteins of 455 and 355 residues, respectively, which are homologs of the DegP protease... - Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and a truncated R1pAS Bass
Department of Molecular Biology, Genentech Inc, South San Francisco, California 94080 4990, USA
J Bacteriol 178:1154-61. 1996..5 centisomes on the chromosome. We propose the names hhoA and hhoB, for htrA homolog, to denote that these genes encode proteins that are 58 and 35% identical, respectively, to the .. - The Escherichia coli sigma(E)-dependent extracytoplasmic stress response is controlled by the regulated proteolysis of an anti-sigma factorS E Ades
Department of Stomatology, University of California at San Francisco, San Francisco, California 94143 USA
Genes Dev 13:2449-61. 1999..We present evidence that the putative inner membrane serine protease, DegS, is responsible for this regulated degradation of RseA. - degS (hhoB) is an essential Escherichia coli gene whose indispensable function is to provide sigma (E) activityB M Alba
Department of Biochemistry, University of California at San Francisco, San Francisco, California 94143, USA
Mol Microbiol 40:1323-33. 2001b>DegS (HhoB), a putative serine protease related to DegP/HtrA, regulates the basal and induced activity of the essential Escherichia coli sigma factor sigma (E), which is involved in the cellular response to extracytoplasmic stress... - YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseAKazue Kanehara
Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
Genes Dev 16:2147-55. 2002..Whereas sigma(E) is thought to be activated by regulated cleavage of RseA on the periplasmic side by the DegS protease, we found that a degradation intermediate of RseA consisting of the transmembrane and the cytoplasmic .. - DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress responseBenjamin M Alba
Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94143, USA
Genes Dev 16:2156-68. 2002..we show that RseA is degraded by sequential proteolytic events controlled by the inner membrane-anchored protease DegS and the membrane-embedded metalloprotease YaeL, an ortholog of mammalian Site-2 protease (S2P)... - A stress sensor for the bacterial periplasmJason C Young
Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
Cell 113:1-2. 2003b>DegS, the periplasmic stress sensor, becomes activated when its PDZ domain recognizes the improperly exposed C-terminal sequences of outer membrane porins... - OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domainNathan P Walsh
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 113:61-71. 2003..This process requires a proteolytic cascade initiated by the DegS protease, which destroys a transmembrane protein (RseA) that normally binds to and inhibits sigma(E)... - Roles of DegP in prevention of protein misfolding in the periplasm upon overexpression of penicillin acylase in Escherichia coliKao Lu Pan
Department of Chemical Engineering, Feng Chia University, Taichung, Taiwan 407, Republic of China
J Bacteriol 185:3020-30. 2003..culture performance upon pac overexpression because coexpression of a DegP-homologous periplasmic protease, DegQ or DegS, could not suppress the physiological toxicity... - DegS is necessary for virulence and is among extraintestinal Escherichia coli genes induced in murine peritonitisPeter Redford
Department of Medical Microbiology and Immunology, University of Wisconsin Madison, 53706 1532, USA
Infect Immun 71:3088-96. 2003..Three lie before open reading frames of unknown function. One promoter is associated with degS, an inner membrane protease... - Regulation of the Escherichia coli sigma-dependent envelope stress responseBenjamin M Alba
Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143 2200, USA
Mol Microbiol 52:613-9. 2004..membrane sigma(E) antisigma factor; RseB, a periplasmic protein that binds to the periplasmic face of RseA; and the DegS and YaeL proteases... - A PDZ switch for a cellular stress responseChristian Schlieker
Zentrum für Molekulare Biologie Heidelberg, Universitat Heidelberg, Im Neuenheimer Feld 282, Heidelberg D 69120, Germany
Cell 117:417-9. 2004..of misfolded porins in the periplasm of bacteria triggers a proteolytic cascade, initiated by activation of DegS, a member of the family of HtrA proteases... - Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a proteaseCorinna Wilken
Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
Cell 117:483-94. 2004..Activation of sigmaE is controlled by a proteolytic cascade that is initiated by the DegS protease... - Allostery is an intrinsic property of the protease domain of DegS: implications for enzyme function and evolutionJungsan Sohn
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Biol Chem 285:34039-47. 2010b>DegS is a periplasmic Escherichia coli protease, which functions as a trimer to catalyze the initial rate-limiting step in a proteolytic cascade that ultimately activates transcription of stress response genes in the cytoplasm... - Identification of major antigenic proteins of Pasteurella piscicidaI Hirono
Laboratory of Genetics and Biochemistry, Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4 5 7, Minato ku, Tokyo, 108, Japan
Microb Pathog 23:371-80. 1997..residues) encodes a homolog of DegQ protease, and the smaller ORF (371 amino acid residues) encodes a homolog of DegS protease. The antibodies reacted with the larger ORF-encoded 45 kDa DegQ homolog protein...