Gene Symbol: TUFM
Description: Tu translation elongation factor, mitochondrial
Alias: elongation factor Tu, mitochondrial, EF-Tu
Hunter S, Spremulli L. Mutagenesis of Arg335 in bovine mitochondrial elongation factor Tu and the corresponding residue in the Escherichia coli factor affects interactions with mitochondrial aminoacyl-tRNAs. RNA Biol. 2004;1:95-102 pubmed
..coli Phe-tRNA.(Phe) These data suggest that the residue at position 287 plays an important role in the binding and EF-Tu-mediated delivery of mitochondrial aa-tRNAs to the A-site of the ribosome. ..
Akama K, Christian B, Jones C, Ueda T, Takeuchi N, Spremulli L. Analysis of the functional consequences of lethal mutations in mitochondrial translational elongation factors. Biochim Biophys Acta. 2010;1802:692-8 pubmed publisher
..coli aa-tRNA but completely inactivates EF-Tu(mt) for binding to mitochondrial aa-tRNA. Clearly the R336Q mutation in EF-Tu(mt) has a far more drastic effect on its interaction with mitochondrial aa-tRNAs than bacterial aa-tRNAs. ..
Woriax V, Burkhart W, Spremulli L. Cloning, sequence analysis and expression of mammalian mitochondrial protein synthesis elongation factor Tu. Biochim Biophys Acta. 1995;1264:347-56 pubmed
..coli as a His-tagged protein and purified to near homogeneity. The expressed form of the factor is active in the poly(U)-directed polymerization of phenylalanine although it is less active than the native EF-Tu.Tsmt complex. ..
Jeppesen M, Navratil T, Spremulli L, Nyborg J. Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex. J Biol Chem. 2005;280:5071-81 pubmed
..The structure of the EF-Tumt.Tsmt complex provides new insights into the nucleotide exchange mechanism and provides a framework for explaining much of the mutational data obtained for this complex. ..
Suzuki H, Ueda T, Taguchi H, Takeuchi N. Chaperone properties of mammalian mitochondrial translation elongation factor Tu. J Biol Chem. 2007;282:4076-84 pubmed
..We discuss the possible role of EF-Tumt chaperone activity in protein quality control in mitochondria, with regard to the recently reported in vivo chaperone function of eEF1A. ..
Ling M, Merante F, Chen H, Duff C, Duncan A, Robinson B. The human mitochondrial elongation factor tu (EF-Tu) gene: cDNA sequence, genomic localization, genomic structure, and identification of a pseudogene. Gene. 1997;197:325-36 pubmed
..2. In addition, an intronless pseudogene of approximately 1.7 kb with 92.6% nucleotide sequence similarity to the EF-Tu gene has also been identified and mapped to chromosome 17q11.2. ..
Andersen G, Thirup S, Spremulli L, Nyborg J. High resolution crystal structure of bovine mitochondrial EF-Tu in complex with GDP. J Mol Biol. 2000;297:421-36 pubmed
..The C-terminal extension of mitochondrial EF-Tu has structural similarities with DNA recognising zinc fingers suggesting that the extension may be involved in recognition of RNA. ..
Karring H, Andersen G, Thirup S, Nyborg J, Spremulli L, Clark B. Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes. Biochim Biophys Acta. 2002;1601:172-7 pubmed
..78 A, c=128.89 A and beta=96.978 degrees. The crystals of the homologous mitochondrial EF-Tu:EF-Ts complex diffract to 4 A and belong to space group C2 with cell parameters a=157.7 A, b=151.9 A, c=156.9 A, and beta=108.96 degrees. ..