Gene Symbol: SPP1
Description: secreted phosphoprotein 1
Alias: OPN, OST, osteopontin, SPP-1, bone sialoprotein 1, bone sialoprotein I, early T-lymphocyte activation 1, osteopontin-K, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
Species: cow
Products:     SPP1

Top Publications

  1. Schnabel R, Kim J, Ashwell M, Sonstegard T, van Tassell C, Connor E, et al. Fine-mapping milk production quantitative trait loci on BTA6: analysis of the bovine osteopontin gene. Proc Natl Acad Sci U S A. 2005;102:6896-901 pubmed
    ..Next, we sequenced a 12.3-kb region harboring Osteopontin, a positional candidate for the statistically most significant of the identified QTL...
  2. Hunter G, Grohe B, Jeffrey S, O Young J, Sørensen E, Goldberg H. Role of phosphate groups in inhibition of calcium oxalate crystal growth by osteopontin. Cells Tissues Organs. 2009;189:44-50 pubmed publisher
    b>Osteopontin (OPN) inhibits the growth of calcium oxalate monohydrate (COM) and other crystal phases in a phosphorylation-dependent manner...
  3. Kerr J, Fisher L, Termine J, Young M. The cDNA cloning and RNA distribution of bovine osteopontin. Gene. 1991;108:237-43 pubmed
    We have isolated and sequenced the bovine cDNA (OPN) counterpart of osteopontin. The cDNA is 1356 nucleotides (nt) in length with an open reading frame of 834 nt, encoding a 278-amino acid (aa) protein...
  4. Salih E, Fluckiger R. Complete topographical distribution of both the in vivo and in vitro phosphorylation sites of bone sialoprotein and their biological implications. J Biol Chem. 2004;279:19808-15 pubmed
    ..Overall, 7 in vitro and 11 in vivo phosphorylation sites were identified unequivocally, with natural variation in the quantitative extent of phosphorylation at each in vivo phosphorylation site. ..
  5. Degrelle S, Campion E, Cabau C, Piumi F, Reinaud P, Richard C, et al. Molecular evidence for a critical period in mural trophoblast development in bovine blastocysts. Dev Biol. 2005;288:448-60 pubmed
    ..Altogether, these results tended to show that early elongation is a critical transition in bovine trophoblast development. ..
  6. Santarelli A, Mascitti M, Orsini G, Memè L, Rocchetti R, Tiriduzzi P, et al. Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells. J Biol Regul Homeost Agents. 2014;28:523-9 pubmed
    ..Both the materials tested in the present study demonstrated a good biocompatibility with the osteoblastic cells allowing, at the same time, the osteogenic differentiation, and they may be useful in clinical use. ..
  7. Poole D, Ndiaye K, Pate J. Expression and regulation of secreted phosphoprotein 1 in the bovine corpus luteum and effects on T lymphocyte chemotaxis. Reproduction. 2013;146:527-37 pubmed publisher
    Secreted phosphoprotein 1 (SPP1) in the bovine corpus luteum (CL) regulates cell function during the transitional periods of luteinization and luteal regression...
  8. White S, Casas E, Allan M, Keele J, Snelling W, Wheeler T, et al. Evaluation in beef cattle of six deoxyribonucleic acid markers developed for dairy traits reveals an osteopontin polymorphism associated with postweaning growth. J Anim Sci. 2007;85:1-10 pubmed
    ..with milk yield or composition, including polymorphisms in secreted phosphoprotein 1 (SPP1; also called osteopontin), growth hormone receptor (GHR), casein S1 (CSN1S1), diacylglycerol O-acyltransferase 1 (DGAT1), peroxisome ..
  9. Mestrović N, Randig O, Abad P, Plohl M, Castagnone Sereno P. Conserved and variable domains in satellite DNAs of mitotic parthenogenetic root-knot nematode species. Gene. 2005;362:44-50 pubmed
    ..javanica and M. arenaria and highly homogenized divergent positions in comparison with the MPA1 indicate similar sequence dynamics in mitotic parthenogenetic taxa to that observed in amphimictic species. ..

More Information


  1. Sørensen E, Petersen T. Purification and characterization of three proteins isolated from the proteose peptone fraction of bovine milk. J Dairy Res. 1993;60:189-97 pubmed
    ..The 60 kDa protein was found to be bovine osteopontin, a very highly phosphorylated protein with an Arg-Gly-Asp sequence which mediates cell attachment.
  2. Salih E, Zhou H, Glimcher M. Phosphorylation of purified bovine bone sialoprotein and osteopontin by protein kinases. J Biol Chem. 1996;271:16897-905 pubmed
    The large number of covalently bound phosphates on the extracellular phosphoproteins osteopontin (OPN) and bone sialoprotein (BSP) have been implicated in biological functions such as mineral deposition and osteoclast binding...
  3. Karcher E, Johnson C, Beitz D, Stabel J. Osteopontin immunoreactivity in the ileum and ileocecal lymph node of dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis. Vet Immunol Immunopathol. 2008;126:142-8 pubmed publisher
    b>Osteopontin (Opn), a highly acidic glycoprotein, promotes cellular adhesion and recruitment and has been shown to be upregulated in the granulomas of mycobacterial infections. Johne's disease, caused by Mycobacterium avium subsp...
  4. Alain K, Karrow N, Thibault C, St Pierre J, Lessard M, Bissonnette N. Osteopontin: an early innate immune marker of Escherichia coli mastitis harbors genetic polymorphisms with possible links with resistance to mastitis. BMC Genomics. 2009;10:444 pubmed publisher
    ..The osteopontin transcript (SPP1) was identified in the somatic cells from cows experimentally infected with Escherichia coli...
  5. Leonard S, Khatib H, Schutzkus V, Chang Y, Maltecca C. Effects of the osteopontin gene variants on milk production traits in dairy cattle. J Dairy Sci. 2005;88:4083-6 pubmed
    b>Osteopontin (OPN) is a highly phosphorylated glycoprotein whose gene has been cloned and sequenced in different species...
  6. Sørensen E, Højrup P, Petersen T. Posttranslational modifications of bovine osteopontin: identification of twenty-eight phosphorylation and three O-glycosylation sites. Protein Sci. 1995;4:2040-9 pubmed
    b>Osteopontin (OPN) is a multiphosphorylated glycoprotein found in bone and other normal and malignant tissues, as well as in the physiological fluids urine and milk...
  7. Schlafer S, Raarup M, Wejse P, Nyvad B, Städler B, Sutherland D, et al. Osteopontin reduces biofilm formation in a multi-species model of dental biofilm. PLoS ONE. 2012;7:e41534 pubmed publisher
    ..We here study the effect of bovine milk osteopontin (OPN), a highly phosphorylated whey glycoprotein, on a multispecies in vitro model of dental biofilm...
  8. Ek Rylander B, Andersson G. Osteoclast migration on phosphorylated osteopontin is regulated by endogenous tartrate-resistant acid phosphatase. Exp Cell Res. 2010;316:443-51 pubmed publisher
    b>Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN...
  9. Erikson D, Burghardt R, Bayless K, Johnson G. Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration. Biol Reprod. 2009;81:814-25 pubmed publisher
    ..Secreted phosphoprotein 1 (SPP1, osteopontin) is induced just before implantation and is present at the conceptus-maternal interface in mammals...
  10. Crivello J, Delvin E. Isolation and characterization of a cDNA for osteopontin-k: a kidney cell adhesion molecule with high homology to osteopontins. J Bone Miner Res. 1992;7:693-9 pubmed
    ..The peptide (osteopontin-k) had a potential glycosylation site (Asn-X-Ser/Thr), a GRGDS receptor binding region, a high level of ..
  11. Kläning E, Christensen B, Sørensen E, Vorup Jensen T, Jensen J. Osteopontin binds multiple calcium ions with high affinity and independently of phosphorylation status. Bone. 2014;66:90-5 pubmed publisher
    b>Osteopontin (OPN) is an acidic, intrinsically disordered extracellular matrix protein with a capacity to modulate biomineralization in vitro and in vivo...
  12. Rodriguez D, Thula Mata T, Toro E, Yeh Y, Holt C, Holliday L, et al. Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts. Acta Biomater. 2014;10:494-507 pubmed publisher
    ..In this study we show that osteopontin (OPN) can similarly serve as a process-directing agent for the intrafibrillar mineralization of collagen, even ..
  13. Karcher E, Bayles D, Bannantine J, Beitz D, Stabel J. Osteopontin: a novel cytokine involved in the regulation of Mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle. J Dairy Sci. 2008;91:3079-91 pubmed publisher
    b>Osteopontin (Opn), an important mediator of the cell-mediated immune response, enhances the host immune response against mycobacterial infections. Infections caused by Mycobacterium avium ssp...
  14. Saad F, Salih E, Glimcher M. Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification. J Cell Biochem. 2008;103:852-6 pubmed
    b>Osteopontin is a noncollagenous, phosphorylated extracellular glycoprotein, expressed in mineralized and nonmineralized tissues, organs and body fluids...
  15. Khatib H, Zaitoun I, Wiebelhaus Finger J, Chang Y, Rosa G. The association of bovine PPARGC1A and OPN genes with milk composition in two independent Holstein cattle populations. J Dairy Sci. 2007;90:2966-70 pubmed
    ..b>Osteopontin (OPN) and peroxisome proliferator activated receptor gamma coactivator 1 alpha (PPARGC1A) are located in the ..
  16. Yamniuk A, Burling H, Vogel H. Thermodynamic characterization of the interactions between the immunoregulatory proteins osteopontin and lactoferrin. Mol Immunol. 2009;46:2395-402 pubmed publisher
    The immunoregulatory proteins osteopontin (OPN) and lactoferrin (LF) are both highly abundant in milk, with a conserved ratio between different mammalian species, suggesting that the role of each protein in infant development may be ..
  17. Sheehy P, Riley L, Raadsma H, Williamson P, Wynn P. A functional genomics approach to evaluate candidate genes located in a QTL interval for milk production traits on BTA6. Anim Genet. 2009;40:492-8 pubmed publisher
    ..These data suggest that the gene product of SPP1 (also known as osteopontin) has a significant role in the modulation of milk protein gene expression...
  18. Souza C, Moura A, Monaco E, Killian G. Binding patterns of bovine seminal plasma proteins A1/A2, 30 kDa and osteopontin on ejaculated sperm before and after incubation with isthmic and ampullary oviductal fluid. Anim Reprod Sci. 2008;105:72-89 pubmed publisher
    ..have reported empirical associations between bovine seminal plasma protein(s) (BSP) A1/A2 and 30 kDa and osteopontin (OPN) in accessory sex gland fluid and bull fertility...
  19. Dudemaine P, Thibault C, Alain K, Bissonnette N. Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk. Anim Genet. 2014;45:629-40 pubmed publisher
    b>Osteopontin (OPN) is now recognized as an important cytokine and extracellular integrin-binding protein at the crossroads of inflammation and homeostasis...
  20. Qi L, Basset C, Averseng O, Quemeneur E, Hagège A, Vidaud C. Characterization of UO2(2+) binding to osteopontin, a highly phosphorylated protein: insights into potential mechanisms of uranyl accumulation in bones. Metallomics. 2014;6:166-76 pubmed publisher
    ..The noncollagenous protein osteopontin (OPN) plays an important role in bone homeostasis...