PRNP

Summary

Gene Symbol: PRNP
Description: prion protein
Alias: AltPrP, PrP, major prion protein, alternative prion protein, major scrapie-associated fibril protein 1, prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia), prion protein PrP, prion protein precursor PrP, prion protein variant a, prion protein variant b
Species: cow
Products:     PRNP

Top Publications

  1. Sander P, Hamann H, Drogemuller C, Kashkevich K, Schiebel K, Leeb T. Bovine prion protein gene (PRNP) promoter polymorphisms modulate PRNP expression and may be responsible for differences in bovine spongiform encephalopathy susceptibility. J Biol Chem. 2005;280:37408-14 pubmed
    ..the variant Creutzfeldt-Jakob disease is greatly influenced by polymorphisms within the human prion protein gene (PRNP). Similar genetic differences exist in sheep, in which PRNP polymorphisms modify the susceptibility to scrapie...
  2. Kues W, Ollhoff R, Carnwath J, de Souza F, Madeira H, Niemann H. High incidence of single nucleotide polymorphisms in the prion protein gene of native Brazilian Caracu cattle. J Anim Breed Genet. 2006;123:326-30 pubmed
    ..However, the pathogenic implications of specific polymorphisms in the bovine prion protein gene (PRNP) are only poorly understood...
  3. Juling K, Schwarzenbacher H, Williams J, Fries R. A major genetic component of BSE susceptibility. BMC Biol. 2006;4:33 pubmed
    Coding variants of the prion protein gene (PRNP) have been shown to be major determinants for the susceptibility to transmitted prion diseases in humans, mice and sheep...
  4. Miyazawa K, Kanaya T, Tanaka S, Takakura I, Watanabe K, Ohwada S, et al. Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice. Histochem Cell Biol. 2007;127:291-301 pubmed
    ..The gastrointestinal tract is thought to be the main site of entry for the pathological isoform of the prion protein (PrP(Sc))...
  5. Nakamura I, Xue G, Sakudo A, Saeki K, Matsumoto Y, Ikuta K, et al. Novel single nucleotide polymorphisms in the specific protein 1 binding site of the bovine PRNP promoter in Japanese Black cattle: impairment of its promoter activity. Intervirology. 2007;50:190-6 pubmed
    Susceptibility to transmissible spongiform encephalopathy and different alleles of the prion protein gene (PRNP) of humans and sheep are associated...
  6. Castilla J, Gutierrez Adan A, Brun A, Pintado B, Parra B, Ramirez M, et al. Different behavior toward bovine spongiform encephalopathy infection of bovine prion protein transgenic mice with one extra repeat octapeptide insert mutation. J Neurosci. 2004;24:2156-64 pubmed
    In humans, insert mutations within the repetitive octapeptide region of the prion protein gene (Prnp) are often associated with familial spongiform encephalopathies...
  7. Sander P, Hamann H, Pfeiffer I, Wemheuer W, Brenig B, Groschup M, et al. Analysis of sequence variability of the bovine prion protein gene (PRNP) in German cattle breeds. Neurogenetics. 2004;5:19-25 pubmed
    Different alleles of the prion protein gene (PRNP) of human and sheep are known to be associated with varying susceptibilities to transmissible spongiform encephalopathies...
  8. Jeong B, Lee Y, Kim N, Carp R, Kim Y. Genotype distribution of the prion protein gene (PRNP) promoter polymorphisms in Korean cattle. Genome. 2006;49:1539-44 pubmed
    ..spongiform encephalopathy (BSE) and insertion/deletion (indel) polymorphisms in the bovine prion protein gene (PRNP) promoter region has been reported in German cattle...
  9. Murdoch B, Clawson M, Yue S, Basu U, McKay S, Settles M, et al. PRNP haplotype associated with classical BSE incidence in European Holstein cattle. PLoS ONE. 2010;5: pubmed publisher
    Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease of cattle. The bovine prion gene (PRNP) contains regions of both high and low linkage disequilibrium (LD) that appear to be conserved across Bos taurus ..
  10. Czarnik U, Zabolewicz T, Strychalski J, Grzybowski G, Bogusz M, Walawski K. Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle. J Appl Genet. 2007;48:69-71 pubmed
    The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp)...

Detail Information

Publications71

  1. Sander P, Hamann H, Drogemuller C, Kashkevich K, Schiebel K, Leeb T. Bovine prion protein gene (PRNP) promoter polymorphisms modulate PRNP expression and may be responsible for differences in bovine spongiform encephalopathy susceptibility. J Biol Chem. 2005;280:37408-14 pubmed
    ..the variant Creutzfeldt-Jakob disease is greatly influenced by polymorphisms within the human prion protein gene (PRNP). Similar genetic differences exist in sheep, in which PRNP polymorphisms modify the susceptibility to scrapie...
  2. Kues W, Ollhoff R, Carnwath J, de Souza F, Madeira H, Niemann H. High incidence of single nucleotide polymorphisms in the prion protein gene of native Brazilian Caracu cattle. J Anim Breed Genet. 2006;123:326-30 pubmed
    ..However, the pathogenic implications of specific polymorphisms in the bovine prion protein gene (PRNP) are only poorly understood...
  3. Juling K, Schwarzenbacher H, Williams J, Fries R. A major genetic component of BSE susceptibility. BMC Biol. 2006;4:33 pubmed
    Coding variants of the prion protein gene (PRNP) have been shown to be major determinants for the susceptibility to transmitted prion diseases in humans, mice and sheep...
  4. Miyazawa K, Kanaya T, Tanaka S, Takakura I, Watanabe K, Ohwada S, et al. Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice. Histochem Cell Biol. 2007;127:291-301 pubmed
    ..The gastrointestinal tract is thought to be the main site of entry for the pathological isoform of the prion protein (PrP(Sc))...
  5. Nakamura I, Xue G, Sakudo A, Saeki K, Matsumoto Y, Ikuta K, et al. Novel single nucleotide polymorphisms in the specific protein 1 binding site of the bovine PRNP promoter in Japanese Black cattle: impairment of its promoter activity. Intervirology. 2007;50:190-6 pubmed
    Susceptibility to transmissible spongiform encephalopathy and different alleles of the prion protein gene (PRNP) of humans and sheep are associated...
  6. Castilla J, Gutierrez Adan A, Brun A, Pintado B, Parra B, Ramirez M, et al. Different behavior toward bovine spongiform encephalopathy infection of bovine prion protein transgenic mice with one extra repeat octapeptide insert mutation. J Neurosci. 2004;24:2156-64 pubmed
    In humans, insert mutations within the repetitive octapeptide region of the prion protein gene (Prnp) are often associated with familial spongiform encephalopathies...
  7. Sander P, Hamann H, Pfeiffer I, Wemheuer W, Brenig B, Groschup M, et al. Analysis of sequence variability of the bovine prion protein gene (PRNP) in German cattle breeds. Neurogenetics. 2004;5:19-25 pubmed
    Different alleles of the prion protein gene (PRNP) of human and sheep are known to be associated with varying susceptibilities to transmissible spongiform encephalopathies...
  8. Jeong B, Lee Y, Kim N, Carp R, Kim Y. Genotype distribution of the prion protein gene (PRNP) promoter polymorphisms in Korean cattle. Genome. 2006;49:1539-44 pubmed
    ..spongiform encephalopathy (BSE) and insertion/deletion (indel) polymorphisms in the bovine prion protein gene (PRNP) promoter region has been reported in German cattle...
  9. Murdoch B, Clawson M, Yue S, Basu U, McKay S, Settles M, et al. PRNP haplotype associated with classical BSE incidence in European Holstein cattle. PLoS ONE. 2010;5: pubmed publisher
    Classical bovine spongiform encephalopathy (BSE) is an acquired prion disease of cattle. The bovine prion gene (PRNP) contains regions of both high and low linkage disequilibrium (LD) that appear to be conserved across Bos taurus ..
  10. Czarnik U, Zabolewicz T, Strychalski J, Grzybowski G, Bogusz M, Walawski K. Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle. J Appl Genet. 2007;48:69-71 pubmed
    The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp)...
  11. Hills D, Comincini S, Schlaepfer J, Dolf G, Ferretti L, Williams J. Complete genomic sequence of the bovine prion gene (PRNP) and polymorphism in its promoter region. Anim Genet. 2001;32:231-2 pubmed
  12. Msalya G, Shimogiri T, Ohno S, Okamoto S, Kawabe K, Minezawa M, et al. Evaluation of PRNP expression based on genotypes and alleles of two indel loci in the medulla oblongata of Japanese Black and Japanese Brown cattle. PLoS ONE. 2011;6:e18787 pubmed publisher
    ..The two cattle breeds were considered differently. Expression of PRNP was significantly (p<0.0001) greater in the homozygous deletion genotype at the 23-bp locus in JB breed...
  13. Xue G, Sakudo A, Kim C, Onodera T. Coordinate regulation of bovine prion protein gene promoter activity by two Sp1 binding site polymorphisms. Biochem Biophys Res Commun. 2008;372:530-5 pubmed publisher
    Relationships between insertion/deletion (Ins/Del) polymorphisms of the bovine prion protein gene (PRNP) promoter and bovine spongiform encephalopathy (BSE) susceptibility have been reported...
  14. Clawson M, Heaton M, Keele J, Smith T, Harhay G, Richt J, et al. A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle. BMC Res Notes. 2008;1:32 pubmed publisher
    ..Distinct bovine prion gene (PRNP) alleles have been associated with classical and atypical BSE susceptibility...
  15. Geldermann H, He H, Bobal P, Bartenschlager H, Preuss S. Comparison of DNA variants in the PRNP and NF1 regions between bovine spongiform encephalopathy and control cattle. Anim Genet. 2006;37:469-74 pubmed
    ..encephalopathy (BSE) cattle and 376 non-diseased control cattle were genotyped for nine loci in the prion protein (PRNP) gene region, three loci in the neurofibromin 1 (NF1) region and four control loci on different chromosomes...
  16. Seabury C, Honeycutt R, Rooney A, Halbert N, Derr J. Prion protein gene (PRNP) variants and evidence for strong purifying selection in functionally important regions of bovine exon 3. Proc Natl Acad Sci U S A. 2004;101:15142-7 pubmed
    Amino acid replacements encoded by the prion protein gene (PRNP) have been associated with transmissible and hereditary spongiform encephalopathies in mammalian species...
  17. Haase B, Doherr M, Seuberlich T, Drogemuller C, Dolf G, Nicken P, et al. PRNP promoter polymorphisms are associated with BSE susceptibility in Swiss and German cattle. BMC Genet. 2007;8:15 pubmed
    Non-synonymous polymorphisms within the prion protein gene (PRNP) influence the susceptibility and incubation time for transmissible spongiform encephalopathies (TSE) in some species such as sheep and humans...
  18. Brunelle B, Greenlee J, Seabury C, Brown C, Nicholson E. Frequencies of polymorphisms associated with BSE resistance differ significantly between Bos taurus, Bos indicus, and composite cattle. BMC Vet Res. 2008;4:36 pubmed publisher
    ..indicus purebred and composite cattle. No novel or TSE-associated PRNP-encoded amino acid polymorphisms were observed for B...
  19. López García F, Zahn R, Riek R, Wuthrich K. NMR structure of the bovine prion protein. Proc Natl Acad Sci U S A. 2000;97:8334-9 pubmed
    ..On the other hand, there are differences between bovine and human PrP in the surface distribution of electrostatic charges, which then appears to be the principal structural feature of ..
  20. Msalya G, Shimogiri T, Nishitani K, Okamoto S, Kawabe K, Minesawa M, et al. Indels within promoter and intron 1 of bovine prion protein gene modulate the gene expression levels in the medulla oblongata of two Japanese cattle breeds. Anim Genet. 2010;41:218-21 pubmed publisher
    Genetic differences which exist in the prion protein gene (PRNP) have been reported to influence susceptibility of humans, sheep and goats to prion diseases...
  21. Brunelle B, Kehrli M, Stabel J, Spurlock D, Hansen L, Nicholson E. Short communication: Allele, genotype, and haplotype data for bovine spongiform encephalopathy-resistance polymorphisms from healthy US Holstein cattle. J Dairy Sci. 2008;91:338-42 pubmed
    ..Based on the frequencies of these regulatory region polymorphisms, the US Holstein population is not at a greater risk for BSE than Holsteins worldwide. ..
  22. Brunelle B, Hamir A, Baron T, Biacabe A, Richt J, Kunkle R, et al. Polymorphisms of the prion gene promoter region that influence classical bovine spongiform encephalopathy susceptibility are not applicable to other transmissible spongiform encephalopathies in cattle. J Anim Sci. 2007;85:3142-7 pubmed
    ..This genetic discrepancy demonstrates that atypical BSE progresses differently in the host relative to classical BSE. These results are consistent with the notion that atypical BSE originates spontaneously in cattle. ..
  23. Shimogiri T, Msalya G, Myint S, Okamoto S, Kawabe K, Tanaka K, et al. Allele distributions and frequencies of the six prion protein gene (PRNP) polymorphisms in Asian native cattle, Japanese breeds, and mythun (Bos frontalis). Biochem Genet. 2010;48:829-39 pubmed publisher
    Six polymorphic sites of the bovine prion protein gene (PRNP) were genotyped in 569 animals of Asian native cattle, Japanese breeds, purebred mythun (Bos frontalis), and mythun x cattle composite animals...
  24. Heaton M, Leymaster K, Freking B, Hawk D, Smith T, Keele J, et al. Prion gene sequence variation within diverse groups of U.S. sheep, beef cattle, and deer. Mamm Genome. 2003;14:765-77 pubmed
    ..Single nucleotide polymorphisms in the sheep prion gene ( PRNP) have been correlated with susceptibility to natural scrapie in some populations...
  25. Clawson M, Heaton M, Keele J, Smith T, Harhay G, Laegreid W. Prion gene haplotypes of U.S. cattle. BMC Genet. 2006;7:51 pubmed
    ..Characterizing linkage disequilibrium (LD) and haplotype networks within the bovine prion gene (PRNP) is important for 1) testing rare or common PRNP variation for an association with BSE and 2) interpreting any ..
  26. Julien O, Chatterjee S, Bjorndahl T, Sweeting B, Acharya S, Semenchenko V, et al. Relative and regional stabilities of the hamster, mouse, rabbit, and bovine prion proteins toward urea unfolding assessed by nuclear magnetic resonance and circular dichroism spectroscopies. Biochemistry. 2011;50:7536-45 pubmed publisher
    ..The effect of the S174N mutation in rabbit PrP(C) was also investigated...
  27. Greenlee J, Smith J, West Greenlee M, Nicholson E. Clinical and pathologic features of H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism. PLoS ONE. 2012;7:e38678 pubmed publisher
    ..While this isolate is categorized as BSE-H due to a higher molecular mass of the unglycosylated PrP(Sc) isoform, a strong labeling of all 3 PrP(Sc) bands with monoclonal antibodies 6H4 and P4, and a second ..
  28. Kuczius T, Karch H, Groschup M. Differential solubility of prions is associated in manifold phenotypes. Mol Cell Neurosci. 2009;42:226-33 pubmed publisher
    The main feature of prion diseases is the accumulation of infectious proteins (PrP(Sc))...
  29. Kaatz M, Fast C, Ziegler U, Balkema Buschmann A, Hammerschmidt B, Keller M, et al. Spread of classic BSE prions from the gut via the peripheral nervous system to the brain. Am J Pathol. 2012;181:515-24 pubmed publisher
    ..between 16 and 44 months post infection (mpi) were examined for the presence of the pathological prion protein (PrP(Sc)) by IHC...
  30. Goldmann W, Hunter N, Martin T, Dawson M, Hope J. Different forms of the bovine PrP gene have five or six copies of a short, G-C-rich element within the protein-coding exon. J Gen Virol. 1991;72 ( Pt 1):201-4 pubmed
    ..bovine spongiform encephalopathy (BSE) have to take into account that structural changes in a host-encoded protein (PrP protein) exhibit an effect on the time course of these diseases and the survival time of any man or animal exposed ..
  31. Espinosa J, Andreoletti O, Castilla J, Herva M, Morales M, Alamillo E, et al. Sheep-passaged bovine spongiform encephalopathy agent exhibits altered pathobiological properties in bovine-PrP transgenic mice. J Virol. 2007;81:835-43 pubmed
    ..Biochemical signature, lesion profile, and PrP(Sc) deposition pattern of both cattle and sheep BSE were similar...
  32. Zhang L, Li N, Fan B, Fang M, Xu W. PRNP polymorphisms in Chinese ovine, caprine and bovine breeds. Anim Genet. 2004;35:457-61 pubmed
    ..Polymorphisms and mutations in the prion protein (PRNP) gene have been associated with the incidence of natural and experimental TSE and the incubation period length...
  33. Forde N, Rogers M, Canty M, Lonergan P, Smith G, Coussens P, et al. Association of the prion protein and its expression with ovarian follicle development in cattle. Mol Reprod Dev. 2008;75:243-9 pubmed
    ..Using both microarray analysis and quantitative real-time PCR, the level of prion protein mRNA (Prnp) in both theca and granulosa cells was measured...
  34. Un C, Oztabak K, Ozdemir N, Tesfaye D, Mengi A, Schellander K. Detection of bovine spongiform encephalopathy-related prion protein gene promoter polymorphisms in local Turkish cattle. Biochem Genet. 2008;46:820-7 pubmed publisher
    Polymorphisms in open reading frames of the prion protein gene (PRNP) have been shown to be associated with prion disease susceptibility in humans, sheep, and mice...
  35. Qin L, Zhao Y, Bao Y, Bai W, Chong J, Zhang G, et al. Polymorphism of the prion protein gene (PRNP) in two Chinese indigenous cattle breeds. Mol Biol Rep. 2011;38:4197-204 pubmed publisher
    Prion protein (PRNP) gene has been located at position q17 of chromosome 13 in cattle. The polymorphisms of PRNP gene might be associated with BSE susceptibility...
  36. Yoshimoto J, Iinuma T, Ishiguro N, Horiuchi M, Imamura M, Shinagawa M. Comparative sequence analysis and expression of bovine PrP gene in mouse L-929 cells. Virus Genes. 1992;6:343-56 pubmed
    A cDNA clone encoding bovine scrapie-associated fibril protein, PrP, from a bovine brain cDNA library and six amplified genomic DNA clones of bovine PrP were characterized...
  37. Imran M, Mahmood S, Babar M, Hussain R, Yousaf M, Abid N, et al. PRNP gene variation in Pakistani cattle and buffaloes. Gene. 2012;505:180-5 pubmed publisher
    ..However, one case of H-type BSE has recently been associated with E211K polymorphism in the prion protein gene (PRNP)...
  38. Choi S, Kim I, Kim D, Kim D, Chae S, Choi H, et al. Comparative genomic organization of the human and bovine PRNP locus. Genomics. 2006;87:598-607 pubmed
    We sequenced a 208-kb BAC clone spanning the bovine prion protein (PRNP) locus, and compared the genomic structure with that of human...
  39. Zhao H, Wang X, Zou W, Zhang Y. Prion protein gene (PRNP) polymorphisms in native Chinese cattle. Genome. 2010;53:138-45 pubmed publisher
    Polymorphisms in four regions of the bovine prion protein gene (PRNP) confer susceptibility to bovine spongiform encephalopathy (BSE)...
  40. Vázquez Fernández E, Vos M, Afanasyev P, Cebey L, Sevillano A, Vidal E, et al. The Structural Architecture of an Infectious Mammalian Prion Using Electron Cryomicroscopy. PLoS Pathog. 2016;12:e1005835 pubmed publisher
    ..PrPSc and its N-terminally truncated variant, PrP 27-30, aggregate into amorphous aggregates, 2D crystals, and amyloid fibrils...
  41. Strychalski J, Czarnik U, Zabolewicz T. Abnormal segregation of alleles and haplotypes at the polymorphic site of the PRNP gene within promoter and intron 1 regions in Polish Holstein-Friesian cattle. Biochem Genet. 2012;50:520-8 pubmed publisher
    ..haplotype segregation at the polymorphic sites within the promoter (23indel) and intron 1 (12indel) regions of the PRNP gene was analyzed in Polish Holstein-Friesian cattle...
  42. Huang T, Xu J, Xiang J, Lu Y, Chen R, Huang L, et al. PrPC interacts with potassium channel tetramerization domain containing 1 (KCTD1) protein through the PrP(51-136) region containing octapeptide repeats. Biochem Biophys Res Commun. 2012;417:182-6 pubmed publisher
    To identify molecular interaction partners of the cellular prion protein (PrP(C)), we applied a yeast two-hybrid screen on a bovine brain cDNA expression library and identified the potassium channel tetramerization domain containing 1 (..
  43. Murakami K, Nishikawa F, Noda K, Yokoyama T, Nishikawa S. Anti-bovine prion protein RNA aptamer containing tandem GGA repeat interacts both with recombinant bovine prion protein and its beta isoform with high affinity. Prion. 2008;2:73-80 pubmed
    ..Following 5'-biotinylation, aptamer #1 specifically detected PrP(c) in bovine brain homogenate in a Northwestern blotting assay...
  44. Elsik C, Tellam R, Worley K, Gibbs R, Muzny D, Weinstock G, et al. The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science. 2009;324:522-8 pubmed publisher
    ..The cattle genome sequence thus provides a resource for understanding mammalian evolution and accelerating livestock genetic improvement for milk and meat production. ..
  45. Meggio F, Negro A, Sarno S, Ruzzene M, Bertoli A, Sorgato M, et al. Bovine prion protein as a modulator of protein kinase CK2. Biochem J. 2000;352 Pt 1:191-6 pubmed
    ..These results disclose the potential of the PrP to modulate the activity of CK2, a pleiotropic protein kinase that is particularly abundant in the brain.
  46. Zhou Z, Yan X, Pan K, Chen J, Xie Z, Xiao G, et al. Fibril formation of the rabbit/human/bovine prion proteins. Biophys J. 2011;101:1483-92 pubmed publisher
    ..The misfolding and conversion of cellular PrP in such mammals into pathogenic PrP is believed to be the key procedure...
  47. Cheng C, Daggett V. Molecular dynamics simulations capture the misfolding of the bovine prion protein at acidic pH. Biomolecules. 2014;4:181-201 pubmed publisher
    ..BSE is caused by the prion protein (PrP), which adopts two conformers; PrPC is the native innocuous form, which is ?-helix rich; and PrPSc is the ?-sheet ..
  48. Davenport K, Henderson D, Bian J, Telling G, Mathiason C, Hoover E. Insights into Chronic Wasting Disease and Bovine Spongiform Encephalopathy Species Barriers by Use of Real-Time Conversion. J Virol. 2015;89:9524-31 pubmed publisher
    ..for transspecies prion transmission is related to the structural characteristics of the enciphering and new host PrP, although the exact mechanism remains incompletely understood...
  49. Wang S, Lv X, Zhang K, Lin T, Liu X, Yuan J, et al. Knockdown of the prion gene expression by RNA interference in bovine fibroblast cells. Mol Biol Rep. 2010;37:3193-8 pubmed publisher
    b>PRNP is the gene encoding prion protein whose misfolded and ?-sheet-rich isoform is the infectious agent of transmissible spongiform encephalopathy (TSE)...
  50. Gurgul A, Polak M, Larska M, Słota E. PRNP and SPRN genes polymorphism in atypical bovine spongiform encephalopathy cases diagnosed in Polish cattle. J Appl Genet. 2012;53:337-42 pubmed publisher
    Polymorphisms in the coding region of the prion protein gene (PRNP) have been associated with the susceptibility and incubation period of prion diseases in humans and sheep...
  51. Thumdee P, Ponsuksili S, Murani E, Nganvongpanit K, Gehrig B, Tesfaye D, et al. Expression of the prion protein gene (PRNP) and cellular prion protein (PrPc) in cattle and sheep fetuses and maternal tissues during pregnancy. Gene Expr. 2007;13:283-97 pubmed
    ..In cattle, prion protein gene (PRNP) transcripts were detected by semiquantitative RT-PCR in reproductive tissues such as ovary, oviduct, endometrium, ..
  52. Maissen M, Roeckl C, Glatzel M, Goldmann W, Aguzzi A. Plasminogen binds to disease-associated prion protein of multiple species. Lancet. 2001;357:2026-8 pubmed
  53. Giles K, Glidden D, Beckwith R, Seoanes R, Peretz D, Dearmond S, et al. Resistance of bovine spongiform encephalopathy (BSE) prions to inactivation. PLoS Pathog. 2008;4:e1000206 pubmed publisher
    ..than 301V prions while Western immunoblotting showed that short acidic SDS treatments reduced protease-resistant PrP(Sc) from BSE prions and 301V prions at similar rates...
  54. Biverståhl H, Andersson A, Gräslund A, Mäler L. NMR solution structure and membrane interaction of the N-terminal sequence (1-30) of the bovine prion protein. Biochemistry. 2004;43:14940-7 pubmed
    ..its position in the membrane may be relevant for the understanding of how the N-terminal (1-30) part of the bovine PrP functions as a cell-penetrating peptide...
  55. Czarnik U, Grzybowski G, Zabolewicz T, Strychalski J, Kaminski S. Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish red cattle, Polish White-backed cattle and European bison (Bison bonasus L., 1758). Genetika. 2009;45:519-25 pubmed
    The aim of the present study was to identify deletion/insertion polymorphism of the bovine prion protein (PRNP) gene within the promoter sequence (23 bp indel), intron 1 (12 bp indel) and the 3' end untranslated region (14 bp indel)...
  56. Elmonir W, Inoshima Y, Elbassiouny A, Ishiguro N. Intron 1 mediated regulation of bovine prion protein gene expression: Role of donor splicing sites, sequences with potential enhancer and suppressor activities. Biochem Biophys Res Commun. 2010;397:706-10 pubmed publisher
    ..Because changes in expression of the prion protein gene (PRNP) alter the incubation time and severity of prion diseases...
  57. Hope J, Reekie L, Hunter N, Multhaup G, Beyreuther K, White H, et al. Fibrils from brains of cows with new cattle disease contain scrapie-associated protein. Nature. 1988;336:390-2 pubmed
    ..They are pathological aggregates of a neuronal membrane protein termed PrP and a protease-resistant form of PrP is a molecular marker of scrapie-associated fibrils...
  58. Vanderperre B, Staskevicius A, Tremblay G, McCoy M, O Neill M, Cashman N, et al. An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein. FASEB J. 2011;25:2373-86 pubmed publisher
    ..The corresponding alternative open reading frame encodes a polypeptide termed alternative prion protein (AltPrP) with a completely different amino acid sequence from PrP...
  59. Liao T, Lee L, Chen R. Leu138 in bovine prion peptide fibrils is involved in seeding discrimination related to codon 129 M/V polymorphism in the prion peptide seeding experiment. FEBS J. 2011;278:4351-61 pubmed publisher
    ..Here, we used a short prion protein (PrP) peptide as a model system to test whether seeding discrimination can be found in this simple system...
  60. Guo M, Huang T, Cui Y, Pan B, Shen A, Sun Y, et al. PrPC interacts with tetraspanin-7 through bovine PrP154-182 containing alpha-helix 1. Biochem Biophys Res Commun. 2008;365:154-7 pubmed
    The cellular prion protein (PrP(C)) is highly conserved in the evolution of mammals, and therefore, thought to have important cellular functions...
  61. Nazabal A, Hornemann S, Aguzzi A, Zenobi R. Hydrogen/deuterium exchange mass spectrometry identifies two highly protected regions in recombinant full-length prion protein amyloid fibrils. J Mass Spectrom. 2009;44:965-77 pubmed publisher
    ..Recently, an in vitro conversion system was established which reproduces the transition of recombinant prion protein PrP(23-230) from its native alpha-helical rich form into an aggregated amyloid beta-sheet rich form with ..
  62. Marcos Z, Bodegas M, Sesma M, Guembe L. Comparative study of PrPc expression in rat, monkey, and cow gastrointestinal tract. Ann N Y Acad Sci. 2005;1040:391-4 pubmed
    The gastrointestinal tract (GIT) appears to be the main site of entry for the pathological isoform of prions (PrP(sc))...
  63. Brunelle B, O Grady A, Nicholson E, Seabury C. Disparate Modes of Evolution Shaped Modern Prion (PRNP) and Prion-Related Doppel (PRND) Variation in Domestic Cattle. PLoS ONE. 2016;11:e0155924 pubmed publisher
    ..the apparent absence of overt behavioral or physiological phenotypes associated with several mammalian prion gene (PRNP) knockout experiments...
  64. Rhie A, Kirby L, Sayer N, Wellesley R, Disterer P, Sylvester I, et al. Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion. J Biol Chem. 2003;278:39697-705 pubmed
    ..To understand the molecular basis of these properties and to distinguish specific from non-specific aptamer-PrP interactions, we studied deletion mutants of bovine PrP in denatured, alpha-helix-rich and beta-sheet-rich forms...
  65. Xue G, Aida Y, Onodera T, Sakudo A. The 5' flanking region and intron1 of the bovine prion protein gene (PRNP) are responsible for negative feedback regulation of the prion protein. PLoS ONE. 2012;7:e32870 pubmed publisher
    ..In the present study, we describe negative feedback regulation of the bovine prion protein (PrP) gene PRNP in Japanese Black cattle...
  66. Prusiner S, Fuzi M, Scott M, Serban D, Serban H, Taraboulos A, et al. Immunologic and molecular biologic studies of prion proteins in bovine spongiform encephalopathy. J Infect Dis. 1993;167:602-13 pubmed
    ..Molecular cloning of the bovine PrP gene showed that it encodes a protein of 256 or 264 amino acids with five or six Gly:Pro-rich octarepeats, ..
  67. Kasai K, Hirata A, Ohyama T, Nokihara K, Yokoyama T, Mohri S. Novel assay with fluorescence-labelled PrP peptides for differentiating L-type atypical and classical BSEs, and scrapie. FEBS Lett. 2012;586:325-9 pubmed publisher
    ..differences of prions may account for the conformational diversity of the pathogenic isoform of prion protein (PrP(Sc)). Here, we applied a protein detection procedure by using fluorescent-labelled peptides for detecting PrP(Sc)...
  68. Sakudo A, Wu G, Onodera T, Ikuta K. Octapeptide repeat region of prion protein (PrP) is required at an early stage for production of abnormal prion protein in PrP-deficient neuronal cell line. Biochem Biophys Res Commun. 2008;365:164-9 pubmed
    An abnormal isoform of prion protein (PrP(Sc)), which is composed of the same amino acids as cellular PrP (PrP(C)) and has proteinase K (PK)-resistance, hypothetically converts PrP(C) into PrP(Sc)...
  69. Maloney B, Ge Y, Greig N, Lahiri D. Presence of a "CAGA box" in the APP gene unique to amyloid plaque-forming species and absent in all APLP-1/2 genes: implications in Alzheimer's disease. FASEB J. 2004;18:1288-90 pubmed publisher