Gene Symbol: LAP3
Description: leucine aminopeptidase 3
Alias: LAP-3, PEPS, cytosol aminopeptidase, LAP, leucyl aminopeptidase, peptidase S, proline aminopeptidase, prolyl aminopeptidase
Species: cow

Top Publications

  1. Cuypers H, van Loon Klaassen L, Egberts W, de Jong W, Bloemendal H. The primary structure of leucine aminopeptidase from bovine eye lens. J Biol Chem. 1982;257:7077-85 pubmed
    ..No significant sequence homology with any other published protein primary structure could be detected. This is the first report of a complete amino acid sequence of an enzyme belonging to the class of two metal peptidases. ..
  2. Cuypers H, van Loon Klaassen L, Egberts W, de Jong W, Bloemendal H. Sulfhydryl content of bovine eye lens leucine aminopeptidase. Determination of the reactivity of the sulfhydryl groups of the zinc metalloenzyme, of the enzyme activated by Mg2+, Mn2+, and Co2+, and of the metal-free apoenzyme. J Biol Chem. 1982;257:7086-91 pubmed
    ..The metal-free apoenzyme has at least three reactive cysteines (residues 344, 412, and 429). The data suggest that sulfhydryl groups are involved in the binding of metal ions. ..
  3. Wallner B, Hession C, Tizard R, Frey A, Zuliani A, Mura C, et al. Isolation of bovine kidney leucine aminopeptidase cDNA: comparison with the lens enzyme and tissue-specific expression of two mRNAs. Biochemistry. 1993;32:9296-301 pubmed
    ..Leucine aminopeptidase (LAP) from bovine lens is the best characterized aminopeptidase and the only LAP for which the amino acid sequence was ..
  4. Zheng X, Ju Z, Wang J, Li Q, Huang J, Zhang A, et al. Single nucleotide polymorphisms, haplotypes and combined genotypes of LAP3 gene in bovine and their association with milk production traits. Mol Biol Rep. 2011;38:4053-61 pubmed publisher
    Leucine aminopeptidase 3 (LAP3) is an aminopeptidase which catalyses the removal of N-terminal amino acids and is involved in protein maturation and degradation...
  5. Strater N, Lipscomb W. Two-metal ion mechanism of bovine lens leucine aminopeptidase: active site solvent structure and binding mode of L-leucinal, a gem-diolate transition state analogue, by X-ray crystallography. Biochemistry. 1995;34:14792-800 pubmed
    ..The Arg-336 side chain interacts with the substrate and the gem-diolate intermediate via water molecules. Near Arg-336 in the b1LAP-leucinal structure, an unusually short hydrogen bond is found between two active site water molecules. ..
  6. Olsen H, Lien S, Gautier M, Nilsen H, Roseth A, Berg P, et al. Mapping of a milk production quantitative trait locus to a 420-kb region on bovine chromosome 6. Genetics. 2005;169:275-83 pubmed
    ..and single- and multipoint analyses, the QTL was positioned to an interval surrounded by the genes ABCG2 and LAP3. As no recombinations were detected in this interval, physical mapping was required for further refining...
  7. Zhu X, Barman A, Ozbil M, Zhang T, Li S, Prabhakar R. Mechanism of peptide hydrolysis by co-catalytic metal centers containing leucine aminopeptidase enzyme: a DFT approach. J Biol Inorg Chem. 2012;17:209-22 pubmed publisher
    ..The results reported in this study have elucidated specific roles of both metal centers, the nucleophile, indirect ligands, and substrates in the catalytic functioning of this important class of binuclear metallopeptidases. ..
  8. Burley S, David P, Sweet R, Taylor A, Lipscomb W. Structure determination and refinement of bovine lens leucine aminopeptidase and its complex with bestatin. J Mol Biol. 1992;224:113-40 pubmed
    ..Finally, the relationship between bovine lens leucine aminopeptidase and the homologous enzyme pepA from Escherichia coli is discussed. ..
  9. Ju Z, Zheng X, Huang J, Qi C, Zhang Y, Li J, et al. Functional characterization of genetic polymorphisms identified in the promoter region of the bovine PEPS gene. DNA Cell Biol. 2012;31:1038-45 pubmed publisher
    b>Peptidase S (PEPS) is a metallopeptidase that cleaves N-terminal residues from proteins and peptides. PEPS is used as a cell maintenance enzyme with critical roles in peptide turnover...

More Information


  1. Burley S, David P, Taylor A, Lipscomb W. Molecular structure of leucine aminopeptidase at 2.7-A resolution. Proc Natl Acad Sci U S A. 1990;87:6878-82 pubmed
    ..Access to this cavity is provided by solvent channels that run along the twofold symmetry axes. ..
  2. Nakamura T, Hinagata J, Tanaka T, Imanishi T, Wada Y, Kodama T, et al. HSP90, HSP70, and GAPDH directly interact with the cytoplasmic domain of macrophage scavenger receptors. Biochem Biophys Res Commun. 2002;290:858-64 pubmed bovine revealed that heat shock protein 90 (HSP90), heat shock protein 70 (HSP70), leucine aminopeptidase (LAP), adenocylhomocysteinase, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were included...
  3. Cappiello M, Alterio V, Amodeo P, Del Corso A, Scaloni A, Pedone C, et al. Metal ion substitution in the catalytic site greatly affects the binding of sulfhydryl-containing compounds to leucyl aminopeptidase. Biochemistry. 2006;45:3226-34 pubmed
    Bovine lens leucyl aminopeptidase (blLAP), a homohexameric metallopeptidase preferring bulky and hydrophobic amino acids at the N-terminus of (di)peptides, contains two Zn(2+) ions per subunit that are essential for catalytic activity...
  4. Takasuga A, Hirotsune S, Itoh R, Jitohzono A, Suzuki H, Aso H, et al. Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36,000 bovine ESTs. Nucleic Acids Res. 2001;29:E108 pubmed
    ..This system is applicable to other organisms, including livestock, for which EST data are limited. ..