CYP11A1

Summary

Gene Symbol: CYP11A1
Description: cytochrome P450, family 11, subfamily A, polypeptide 1
Alias: CYP11A, CYPXIA1, P450(scc), cholesterol side-chain cleavage enzyme, mitochondrial, cholesterol desmolase, cytochrome P450 11A1, cytochrome P450(scc)
Species: cow
Products:     CYP11A1

Top Publications

  1. Slominski A, Semak I, Wortsman J, Zjawiony J, Li W, Zbytek B, et al. An alternative pathway of vitamin D metabolism. Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2. FEBS J. 2006;273:2891-901 pubmed
    ..hydroxylating pathway for the metabolism of vitamin D2 in a cytochrome P450 side chain cleavage (P450scc; CYP11A1) reconstituted system...
  2. Schiffler B, Zöllner A, Bernhardt R. Kinetic and optical biosensor study of adrenodoxin mutant AdxS112W displaying an enhanced interaction towards the cholesterol side chain cleavage enzyme (CYP11A1). Eur Biophys J. 2011;40:1275-82 pubmed publisher
    In mammals, steroid hormones are synthesized from cholesterol that is metabolized by the mitochondrial CYP11A1 system leading to pregnenolone...
  3. Morohashi K, Fujii Kuriyama Y, Okada Y, Sogawa K, Hirose T, Inayama S, et al. Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex. Proc Natl Acad Sci U S A. 1984;81:4647-51 pubmed
    ..Only two cysteinyl residues are present in the mature form of P-450(SCC), one of which is located in the middle of the conserved sequence, confirming the function of this cysteinyl residue as the fifth ligand of the heme. ..
  4. Ogishima T, Okada Y, Kominami S, Takemori S, Omura T. Partial amino acid sequences of two mitochondrial and two microsomal cytochrome P-450's from adrenal cortex. J Biochem. 1983;94:1711-4 pubmed
    ..These findings strongly suggest that the NH2-terminal portions of newly synthesized P-450's determine their intracellular localization to different cell organelles. ..
  5. Guryev O, Carvalho R, Usanov S, Gilep A, Estabrook R. A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1). Proc Natl Acad Sci U S A. 2003;100:14754-9 pubmed
    ..In this article, we describe in vitro experiments using purified mitochondrial cytochrome P450scc (CYP11A1) reconstituted with the iron-sulfer protein, adrenodoxin, and the flavoprotein, adrenodoxin reductase, and show ..
  6. Heo G, Liao W, Turko I, Pikuleva I. Features of the retinal environment which affect the activities and product profile of cholesterol-metabolizing cytochromes P450 CYP27A1 and CYP11A1. Arch Biochem Biophys. 2012;518:119-26 pubmed publisher
    ..The results provide insight into the retina's regulation of the enzyme-mediated cholesterol removal. ..
  7. Tuckey R, Nguyen M, Slominski A. Kinetics of vitamin D3 metabolism by cytochrome P450scc (CYP11A1) in phospholipid vesicles and cyclodextrin. Int J Biochem Cell Biol. 2008;40:2619-26 pubmed publisher
    Vitamin D3 can be hydroxylated sequentially by cytochrome P450scc (CYP11A1) producing 20-hydroxyvitamin D3, 20,23-dihydroxyvitamin D3 and 17,20,23-trihydroxyvitamin D3...
  8. Irving Rodgers H, Harland M, Sullivan T, Rodgers R. Studies of granulosa cell maturation in dominant and subordinate bovine follicles: novel extracellular matrix focimatrix is co-ordinately regulated with cholesterol side-chain cleavage CYP11A1. Reproduction. 2009;137:825-34 pubmed publisher
    ..Changes in the expression of FSH receptor, LH receptor, cholesterol side-chain cleavage (CYP11A1), 3beta-hydroxysteroid dehydrogenase, aromatase (CYP19A1) and inhibin-alpha and beta-B were observed as expected ..
  9. Pikuleva I, Mast N, Liao W, Turko I. Studies of membrane topology of mitochondrial cholesterol hydroxylases CYPs 27A1 and 11A1. Lipids. 2008;43:1127-32 pubmed publisher
    ..b>CYP11A1 is a steroidogenic P450 that converts cholesterol to pregnenolone, the first step in the biosynthesis of all ..

More Information

Publications18

  1. Mosa A, Neunzig J, Gerber A, Zapp J, Hannemann F, Pilak P, et al. 2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation. J Steroid Biochem Mol Biol. 2015;150:1-10 pubmed publisher
    The biosynthesis of steroid hormones in vertebrates is initiated by the cytochrome P450 CYP11A1, which performs the side-chain cleavage of cholesterol thereby producing pregnenolone...
  2. Tuckey R, Slominski A, Cheng C, Chen J, Kim T, Xiao M, et al. Lumisterol is metabolized by CYP11A1: discovery of a new pathway. Int J Biochem Cell Biol. 2014;55:24-34 pubmed publisher
    ..The aim of this study was to test the ability of CYP11A1 to metabolize L3...
  3. Vijayakumar S, Salerno J. Molecular modeling of the 3-D structure of cytochrome P-450scc. Biochim Biophys Acta. 1992;1160:281-6 pubmed
    ..The most hydrophobic of these four external loops is proposed as a candidate for membrane attachment. ..
  4. Schiffler B, Zöllner A, Bernhardt R. Stripping down the mitochondrial cholesterol hydroxylase system, a kinetics study. J Biol Chem. 2004;279:34269-76 pubmed
    The origin of steroid hormones in mammals is cholesterol that is metabolized by the mitochondrial CYP11A1 system...
  5. Chashchin V, Lapko V, Adamovich T, Lapko A, Kuprina N, Akhrem A. Primary structure of the cholesterol side-chain cleavage cytochrome P-450 from bovine adrenocortical mitochondria and some aspects of its functioning on a structural level. Biochim Biophys Acta. 1986;871:217-23 pubmed
    ..It is shown that the transformation of P-450scc to P-420 is accompanied by the appearance of an additional trypsin-sensitive peptide bond in the N-terminal part of P-450scc. ..
  6. Ahlgren R, Simpson E, Waterman M, Lund J. Characterization of the promoter/regulatory region of the bovine CYP11A (P-450scc) gene. Basal and cAMP-dependent expression. J Biol Chem. 1990;265:3313-9 pubmed
    The promoter/regulatory region of the bovine CYP11A (P-450scc) gene was cloned from a bovine genomic library...
  7. Janocha S, Bichet A, Zöllner A, Bernhardt R. Substitution of lysine with glutamic acid at position 193 in bovine CYP11A1 significantly affects protein oligomerization and solubility but not enzymatic activity. Biochim Biophys Acta. 2011;1814:126-31 pubmed publisher
    b>CYP11A1, a mitochondrial cytochrome P450, catalyzes the conversion from cholesterol to pregnenolone, the crucial step in the steroid hormone biosynthesis of mammals...
  8. Mast N, Annalora A, Lodowski D, Palczewski K, Stout C, Pikuleva I. Structural basis for three-step sequential catalysis by the cholesterol side chain cleavage enzyme CYP11A1. J Biol Chem. 2011;286:5607-13 pubmed publisher
    Mitochondrial cytochrome P450 11A1 (CYP11A1 or P450 11A1) is the only known enzyme that cleaves the side chain of cholesterol, yielding pregnenolone, the precursor of all steroid hormones...
  9. Waterman M, Kagawa N, Zanger U, Momoi K, Lund J, Simpson E. Comparison of cAMP-responsive DNA sequences and their binding proteins associated with expression of the bovine CYP17 and CYP11A and human CYP21B genes. J Steroid Biochem Mol Biol. 1992;43:931-5 pubmed publisher
    ..upstream regions, the genes encoding the steroid hydroxylases P450c17, P450c21 and P450scc (CYP17, CYP21B and CYP11A, respectively) were found to contain unique cAMP-responsive sequences (CRSs)...