CAST

Summary

Gene Symbol: CAST
Description: calpastatin
Alias: calpastatin, calpain inhibitor, calpastatin isoform III, calpastatin type I, calpastatin type II, calpastatin type III, calpastatin type IV
Species: cow
Products:     CAST

Top Publications

  1. Parr T, Bardsley R, Gilmour R, Buttery P. Changes in calpain and calpastatin mRNA induced by beta-adrenergic stimulation of bovine skeletal muscle. Eur J Biochem. 1992;208:333-9 pubmed
    ..in the activity of the calcium dependent neutral proteinases (calpains I and II) and their specific inhibitor (calpastatin) accompany beta-agonist-induced hypertrophy...
  2. Cong M, Thompson V, Goll D, Antin P. The bovine calpastatin gene promoter and a new N-terminal region of the protein are targets for cAMP-dependent protein kinase activity. J Biol Chem. 1998;273:660-6 pubmed
    To investigate the regulation of calpastatin gene expression, we isolated bovine heart calpastatin cDNAs and 5'-regions of the calpastatin gene...
  3. Singh N, Shrivastav A, Olson D, Lakshmikuttyamma A, Ross A, Parr T, et al. Cardiac high molecular weight calmodulin-binding protein is homologous to calpastatin I and calpastatin II. Biochem Biophys Res Commun. 2008;373:387-91 pubmed publisher
    b>Calpastatin is an endogenous inhibitor of calpain, which has been implicated in various physiological and pathological processes...
  4. Killefer J, Koohmaraie M. Bovine skeletal muscle calpastatin: cloning, sequence analysis, and steady-state mRNA expression. J Anim Sci. 1994;72:606-14 pubmed
    b>Calpastatin is a specific inhibitor of the calpains. Calpains play a key role in postmortem tenderization of meat and have been hypothesized to be involved in muscle protein degradation in living tissue...
  5. Enriquez Valencia C, Pereira G, Malheiros J, de Vasconcelos Silva J, Albuquerque L, de Oliveira H, et al. Effect of the g.98535683A>G SNP in the CAST gene on meat traits of Nellore beef cattle (Bos indicus) and their crosses with Bos taurus. Meat Sci. 2017;123:64-66 pubmed publisher
    The objective of this study was to estimate allele frequencies of the g.98535683A>G:BTAU7 SNP in the CAST gene in different genetic groups of beef cattle produced in Brazil (Nellore and their crosses with Bos taurus), and to evaluate ..
  6. Raynaud P, Jayat Vignoles C, Laforet M, Leveziel H, Amarger V. Four promoters direct expression of the calpastatin gene. Arch Biochem Biophys. 2005;437:69-77 pubmed
    b>Calpastatin is a specific endogenous protein inhibitor of the ubiquitous calcium dependent proteinases mu- and m-calpain. The calpain-calpastatin system is involved in various physiological and pathological processes...
  7. Ribeca C, Bonfatti V, Cecchinato A, Albera A, Maretto F, Gallo L, et al. Association of polymorphisms in calpain 1, (mu/I) large subunit, calpastatin, and cathepsin D genes with meat quality traits in double-muscled Piemontese cattle. Anim Genet. 2013;44:193-6 pubmed publisher
    Five single-nucleotide polymorphisms (SNPs) located in the calpain 1, (mu/I) large subunit (CAPN1), calpastatin (CAST), and cathepsin D (CTSD) genes were analyzed in a large sample of Piemontese cattle...
  8. Chung H, Davis M. Effects of genetic variants for the calpastatin gene on calpastatin activity and meat tenderness in Hanwoo (Korean cattle). Meat Sci. 2012;90:711-4 pubmed publisher
    This study was designed to investigate the effects of calpastatin genotypes determined by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) on calpastatin activity (CAC) and Warner-Bratzler Shear Force (WBS)...
  9. Kar P, Chakraborti T, Roy S, Choudhury R, Chakraborti S. Identification of calpastatin and mu-calpain and studies of their association in pulmonary smooth muscle mitochondria. Arch Biochem Biophys. 2007;466:290-9 pubmed
    Using calpastatin antibody we have identified a 145 kDa major band along with two relatively minor bands at 120 kDa and 110 kDa calpastatin molecules in bovine pulmonary artery smooth muscle mitochondria...

More Information

Publications28

  1. Allais S, Journaux L, Leveziel H, Payet Duprat N, Raynaud P, Hocquette J, et al. Effects of polymorphisms in the calpastatin and ?-calpain genes on meat tenderness in 3 French beef breeds. J Anim Sci. 2011;89:1-11 pubmed publisher
    ..of the study were to evaluate allelic frequencies and to test the association of polymorphisms in the calpastatin (CAST) and µ-calpain (CAPN1) genes with meat tenderness in 3 French beef breeds...
  2. Ba H, Reddy B, Hwang I. Role of calpastatin in the regulation of mRNA expression of calpain, caspase, and heat shock protein systems in bovine muscle satellite cells. In Vitro Cell Dev Biol Anim. 2015;51:447-54 pubmed publisher
    b>Calpastatin participates in apoptotic cell death and cell signaling, but its role in skeletal myoblast development and molecular involvements in cell growth still remains unknown...
  3. Chai H, Lim D, Jung E, Choi B, Cho Y. Understanding the interaction determinants of CAPN1 inhibition by CAST4 from bovines using molecular modeling techniques. Molecules. 2014;19:14316-51 pubmed publisher
    ..This is also thought to be associated with the effects of either small CAPN inhibitors or the natural inhibitor CAST. They mainly bind to the binding site of CAPN substrate proteins and competitively inhibit the binding of the ..
  4. Niciura S, Ibelli A, Gouveia G, Gromboni J, Rocha M, de Souza M, et al. Polymorphism and parent-of-origin effects on gene expression of CAST, leptin and DGAT1 in cattle. Meat Sci. 2012;90:507-10 pubmed publisher
    ..b>CAST, related to meat tenderness, and DGAT1 and leptin, related to fat deposition, were evaluated...
  5. Pinto L, Ferraz J, Meirelles F, Eler J, Rezende F, Carvalho M, et al. Association of SNPs on CAPN1 and CAST genes with tenderness in Nellore cattle. Genet Mol Res. 2010;9:1431-42 pubmed
    We examined whether single-nucleotide polymorphisms (SNPs) in the calpain (CAPN) and calpastatin (CAST) genes, described from Bos primigenius taurus, are polymorphic in Nellore cattle...
  6. Juszczuk Kubiak E, Wyszynska Koko J, Wicinska K, Rosochacki S. A novel polymorphisms in intron 12 of the bovine calpastatin gene. Mol Biol Rep. 2008;35:29-35 pubmed
    b>Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases-mu-calpain and m-calpain, found in mammalian tissues...
  7. Samanta K, Kar P, Chakraborti T, Shaikh S, Chakraborti S. Characteristic properties of endoplasmic reticulum membrane m-calpain, calpastatin and lumen m-calpain: a comparative study between membrane and lumen m-calpains. J Biochem. 2010;147:765-79 pubmed publisher
    ..that bovine pulmonary smooth muscle endoplasmic reticulum (ER) membrane possesses associated m-calpain and calpastatin and ER lumen contains only m-calpain...
  8. Kar P, Chakraborti T, Samanta K, Chakraborti S. Submitochondrial localization of associated mu-calpain and calpastatin. Arch Biochem Biophys. 2008;470:176-86 pubmed
    Recently, we have reported the presence of calpain-calpastatin system in mitochondria of bovine pulmonary smooth muscle [P. Kar, T. Chakraborti, S. Roy, R. Choudhury, S. Chakraborti, Arch. Biochem. Biophys. 466 (2007) 290-299]...
  9. Garcia M, Michal J, Gaskins C, Reeves J, Ott T, Liu Y, et al. Significant association of the calpastatin gene with fertility and longevity in dairy cattle. Anim Genet. 2006;37:304-5 pubmed
  10. Calvo J, Iguácel L, Kirinus J, Serrano M, Ripoll G, Casasús I, et al. A new single nucleotide polymorphism in the calpastatin (CAST) gene associated with beef tenderness. Meat Sci. 2014;96:775-82 pubmed publisher
    ..Thr182Ala (NM_174003). This amino acid substitution could affect the interacting regions between the calpastatin L-domain and calpain, and then could generate a more stable union between calpain and calpastatin.
  11. Mellgren R, Lane R, Mericle M. The binding of large calpastatin to biologic membranes is mediated in part by interaction of an amino terminal region with acidic phospholipids. Biochim Biophys Acta. 1989;999:71-7 pubmed
    ..The calcium-dependent proteinases (calpains) co-exist in cells with a specific protein inhibitor called calpastatin. Distribution of this inhibitor to different subcellular sites could be important in overall regulation of the ..
  12. Li Y, Jin H, Yan C, Seo K, Zhang L, Ren C, et al. Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China. Mol Biol Rep. 2013;40:1875-81 pubmed publisher
    Bovine calpastatin (CAST) gene polymorphisms have been associated with meat tenderness traits; however, little is known about how the CAST gene affects beef quality traits...
  13. Tait R, Shackelford S, Wheeler T, King D, Casas E, Thallman R, et al. ?-Calpain, calpastatin, and growth hormone receptor genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in Angus cattle selected to increase minor haplotype and allele frequencies. J Anim Sci. 2014;92:456-66 pubmed publisher
    ..Divergent haplotypes within µ-calpain (CAPN1), and SNP within calpastatin (CAST) and growth hormone receptor (GHR) were successfully selected to increase their frequencies...
  14. Nattrass G, Cafe L, McIntyre B, Gardner G, McGilchrist P, Robinson D, et al. A post-transcriptional mechanism regulates calpastatin expression in bovine skeletal muscle. J Anim Sci. 2014;92:443-55 pubmed publisher
    ..to investigate whether single nucleotide polymorphisms (SNP) in the calpain 1 (CAPN1), calpain 3 (CAPN3) and calpastatin (CAST) genes, which have been shown to be associated with shear force and tenderness differences in the ..
  15. Chai H, Lim D, Lee S, Chai H, Jung E. Homology modeling study of bovine ?-calpain inhibitor-binding domains. Int J Mol Sci. 2014;15:7897-938 pubmed publisher
    The activated mammalian CAPN-structures, the CAPN/CAST complex in particular, have become an invaluable target model using the structure-based virtual screening of drug candidates from the discovery phase to development for over-activated ..
  16. Castro S, Ríos M, Ortiz Y, Manrique C, Jiménez A, Ariza F. Association of single nucleotide polymorphisms in CAPN1, CAST and MB genes with meat color of Brahman and crossbreed cattle. Meat Sci. 2016;117:44-9 pubmed publisher
    The objective of this research was to determine the association of SNPs in the candidate genes Calpain (CAPN1), Calpastatin (CAST) and Myoglobin (MB) with colorimetric parameters (L *, a *, b *, C *, hue) in a F1 population (n = 164) ..
  17. Schenkel F, Miller S, Jiang Z, Mandell I, Ye X, Li H, et al. Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. J Anim Sci. 2006;84:291-9 pubmed
    b>Calpastatin (CAST) is a naturally occurring protein that inhibits the normal tenderization of meat as it ages postmortem...
  18. Tait R, Shackelford S, Wheeler T, King D, Keele J, Casas E, et al. CAPN1, CAST, and DGAT1 genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in a beef cattle population selected for haplotype and allele equalization. J Anim Sci. 2014;92:5382-93 pubmed publisher
    ..Three haplotypes within μ-calpain (CAPN1), a SNP in calpastatin (CAST), and a dinucleotide substitution in diacylglycerol O-acyltransferase 1 (DGAT1) were successfully ..
  19. Cummins K, Lonergan S, Huff Lonergan E. Short commuunication: Effect of dietary protein depletion and repletion on skeletal muscle calpastatin during early lactation. J Dairy Sci. 2004;87:1428-31 pubmed
    ..Milk production, DMI, and milk component contents were measured. Calpain and calpastatin contents of muscle taken at biopsy were evaluated using Western blotting techniques...