CALM

Summary

Gene Symbol: CALM
Description: calmodulin-like
Alias: CALM2, calmodulin, caM, calmodulin 2 (phosphorylase kinase, delta)
Species: cow
Products:     CALM

Top Publications

  1. Hoshino M, Nakamura S. Small GTPase Rin induces neurite outgrowth through Rac/Cdc42 and calmodulin in PC12 cells. J Cell Biol. 2003;163:1067-76 pubmed
    The novel Ras-like small GTPase Rin is expressed prominently in adult neurons, and binds calmodulin (CaM) through its COOH-terminal-binding motif...
  2. Bussemer J, Chigri F, Vothknecht U. Arabidopsis ATPase family gene 1-like protein 1 is a calmodulin-binding AAA+-ATPase with a dual localization in chloroplasts and mitochondria. FEBS J. 2009;276:3870-80 pubmed publisher
    ..Using affinity chromatography on calmodulin-agarose with chloroplast proteins, we purified a 50 kDa protein encoded by AT4G30490 with similarity to the ..
  3. Majava V, Wang C, Myllykoski M, Kangas S, Kang S, Hayashi N, et al. Structural analysis of the complex between calmodulin and full-length myelin basic protein, an intrinsically disordered molecule. Amino Acids. 2010;39:59-71 pubmed publisher
    ..One of the best-characterized protein ligands for MBP is calmodulin (CaM), a highly acidic calcium sensor...
  4. Reizes O, Barylko B, Li C, Sudhof T, Albanesi J. Domain structure of a mammalian myosin I beta. Proc Natl Acad Sci U S A. 1994;91:6349-53 pubmed
    ..A gel overlay method was used to confirm that calmodulin binds to the consensus calmodulin-binding sequence in MMI beta...
  5. Crouch T, Klee C. Positive cooperative binding of calcium to bovine brain calmodulin. Biochemistry. 1980;19:3692-8 pubmed
    Equilibrium dialysis measurements of the binding of Ca2+ to calmodulin have confirmed the existence of four high affinity Ca2+-binding sites (Kd between 3 X 10(-6) and 2 X 10(-5) M)...
  6. Tirone F, Cox J. NADPH oxidase 5 (NOX5) interacts with and is regulated by calmodulin. FEBS Lett. 2007;581:1202-8 pubmed
    ..The C terminus contains a consensus calmodulin-binding domain (CaMBD), which, however, is not the binding site of the N-terminal regulatory domain...
  7. Sigalas C, Bent S, Kitmitto A, O NEILL S, Sitsapesan R. Ca(2+)-calmodulin can activate and inactivate cardiac ryanodine receptors. Br J Pharmacol. 2009;156:794-806 pubmed publisher
    Ca(2+)-calmodulin (Ca(2+)CaM) is widely accepted as an inhibitor of cardiac ryanodine receptors (RyR2); however, the effects of physiologically relevant CaM concentrations have not been fully investigated...
  8. Sharp J, Tomer K. Analysis of the oxidative damage-induced conformational changes of apo- and holocalmodulin by dose-dependent protein oxidative surface mapping. Biophys J. 2007;92:1682-92 pubmed
    b>Calmodulin (CaM) is known to undergo conformational and functional changes on oxidation, allowing CaM to function as an oxidative stress sensor...
  9. He Y, Haque M, Stuehr D, Lu H. Single-molecule spectroscopy reveals how calmodulin activates NO synthase by controlling its conformational fluctuation dynamics. Proc Natl Acad Sci U S A. 2015;112:11835-40 pubmed publisher
    ..Although NOS catalysis relies on domain motions, and is activated by calmodulin binding, the relationships are unclear...

More Information

Publications50

  1. Strynadka N, James M. Two trifluoperazine-binding sites on calmodulin predicted from comparative molecular modeling with troponin-C. Proteins. 1988;3:1-17 pubmed
    Among the known regulatory proteins that are conformationally sensitive to the binding of calcium ions, calmodulin and troponin-C have the greatest primary sequence homology...
  2. Meador W, Means A, Quiocho F. Modulation of calmodulin plasticity in molecular recognition on the basis of x-ray structures. Science. 1993;262:1718-21 pubmed
    b>Calmodulin is the primary calcium-dependent signal transducer and regulator of a wide variety of essential cellular functions...
  3. Arnett D, Persechini A, Tran Q, Black D, Johnson C. Fluorescence quenching studies of structure and dynamics in calmodulin-eNOS complexes. FEBS Lett. 2015;589:1173-8 pubmed publisher
    Activation of endothelial nitric oxide synthase (eNOS) by calmodulin (CaM) facilitates formation of a sequence of conformational states that is not well understood...
  4. Bentrop D, Bertini I, Cremonini M, Forsen S, Luchinat C, Malmendal A. Solution structure of the paramagnetic complex of the N-terminal domain of calmodulin with two Ce3+ ions by 1H NMR. Biochemistry. 1997;36:11605-18 pubmed
    The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, ..
  5. Wall M, Clarage J, Phillips G. Motions of calmodulin characterized using both Bragg and diffuse X-ray scattering. Structure. 1997;5:1599-612 pubmed
    b>Calmodulin is a calcium-activated regulatory protein which can bind to many different targets. The protein resembles a highly flexible dumbbell, and bends in the middle as it binds...
  6. Nunomura W, Takakuwa Y, Parra M, Conboy J, Mohandas N. Ca(2+)-dependent and Ca(2+)-independent calmodulin binding sites in erythrocyte protein 4.1. Implications for regulation of protein 4.1 interactions with transmembrane proteins. J Biol Chem. 2000;275:6360-7 pubmed
    In vitro protein binding assays identified two distinct calmodulin (CaM) binding sites within the NH(2)-terminal 30-kDa domain of erythrocyte protein 4.1 (4...
  7. Herbst S, Masada N, Pfennig S, Ihling C, Cooper D, Sinz A. Structural insights into calmodulin/adenylyl cyclase 8 interaction. Anal Bioanal Chem. 2013;405:9333-42 pubmed publisher
    b>Calmodulin (CaM) is a highly conserved intracellular Ca(2+)-binding protein that exerts important functions in many cellular processes...
  8. Boehning D, Sedaghat L, Sedlak T, Snyder S. Heme oxygenase-2 is activated by calcium-calmodulin. J Biol Chem. 2004;279:30927-30 pubmed
    ..Through a yeast two-hybrid screen we identified calmodulin as a potential regulator of HO2 activity...
  9. Chaudhuri P, Rosenbaum M, Sinharoy P, Damron D, Birnbaumer L, Graham L. Membrane translocation of TRPC6 channels and endothelial migration are regulated by calmodulin and PI3 kinase activation. Proc Natl Acad Sci U S A. 2016;113:2110-5 pubmed publisher
    ..The precise mechanism through which lysoPC activates TRPC6 channels is not known, but calmodulin (CaM) contributes to the regulation of TRPC channels...
  10. Martinez J, Moeller I, Erdjument Bromage H, Tempst P, Lauring B. Parkinson's disease-associated alpha-synuclein is a calmodulin substrate. J Biol Chem. 2003;278:17379-87 pubmed
    ..We detected several cross-links and purified and identified one as calmodulin (CaM). CaM binds to both wild type and PD-associated mutant alpha-synucleins in a calcium-dependent manner...
  11. Olsson L, Sjölin L. Structure of Escherichia coli fragment TR2C from calmodulin to 1.7 A resolution. Acta Crystallogr D Biol Crystallogr. 2001;57:664-9 pubmed
    Fragment TR2C is the C-terminal part of the calcium-binding protein calmodulin, including residues 78-148. The crystal structure of TR2C was solved by molecular replacement and refined to a conventional R value of 21.8% (R(free) = 22...
  12. de Lillo A, Tejerina J, Fierro J. Interaction of calmodulin with lactoferrin. FEBS Lett. 1992;298:195-8 pubmed
    b>Calmodulin, as a major intracellular calcium-binding protein, regulates many Ca(2+)-dependent enzymes and plays an important role in a wide spectrum of cellular functions of the eukaryotes...
  13. Vandonselaar M, Hickie R, Quail J, Delbaere L. Trifluoperazine-induced conformational change in Ca(2+)-calmodulin. Nat Struct Biol. 1994;1:795-801 pubmed
    ..that, as a consequence of binding the drug trifluoperazine, a major conformational movement occurs in Ca(2+)-calmodulin (CaM)...
  14. Popescu S, Popescu G, Bachan S, Zhang Z, Seay M, Gerstein M, et al. Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays. Proc Natl Acad Sci U S A. 2007;104:4730-5 pubmed
    Calmodulins (CaMs) are the most ubiquitous calcium sensors in eukaryotes. A number of CaM-binding proteins have been identified through classical methods, and many proteins have been predicted to bind CaMs based on their structural ..
  15. Lu Z, Shen M, Cao Y, Zhang H, Yao L, Li X. Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a. J Biol Chem. 2012;287:16530-40 pubmed publisher
    ..the actin-activated ATPase activity of myosin 5a is stimulated by micromolar concentrations of Ca(2+) and that calmodulin (CaM) binds to IQ motifs of the myosin 5a heavy chain indicates that Ca(2+) regulates myosin 5a function via ..
  16. McMurry J, Chrestensen C, Scott I, Lee E, Rahn A, Johansen A, et al. Rate, affinity and calcium dependence of nitric oxide synthase isoform binding to the primary physiological regulator calmodulin. FEBS J. 2011;278:4943-54 pubmed publisher
    ..biosensors the binding and release of endothelial and neuronal nitric oxide synthase (eNOS and nNOS) from calmodulin (CaM) was measured...
  17. Wu L, Xu L, Liao J, Chen J, Liang Y. Both the C-terminal polylysine region and the farnesylation of K-RasB are important for its specific interaction with calmodulin. PLoS ONE. 2011;6:e21929 pubmed publisher
    ..There is considerable evidence showing that calmodulin (CaM) binds to K-RasB and dissociates K-RasB from membrane and that the inactivation of CaM is able to induce K-..
  18. Jamieson G, Frazier W. Dictyostelium calmodulin: affinity isolation and characterization. Arch Biochem Biophys. 1983;227:609-17 pubmed
    The Ca2+-binding regulatory protein calmodulin (CaM) has been purified from the cellular slime mold, Dictyostelium discoideum...
  19. Watterson D, Sharief F, Vanaman T. The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain. J Biol Chem. 1980;255:962-75 pubmed
  20. Li H, Sánchez Torres J, del Carpio A, Nogales González A, Molina Ortiz P, Moreno M, et al. The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7. Oncogene. 2005;24:4206-19 pubmed
    We demonstrate using Ca2+-dependent calmodulin (CaM)-affinity chromatography and overlay with biotinylated CaM that the adaptor proteins growth factor receptor bound (Grb)7 and Grb7V (a naturally occurring variant lacking the Src homology ..
  21. San José E, Benguria A, Geller P, Villalobo A. Calmodulin inhibits the epidermal growth factor receptor tyrosine kinase. J Biol Chem. 1992;267:15237-45 pubmed
    We demonstrate in this report that the epidermal growth factor (EGF) receptor from rat liver can be isolated by calmodulin affinity chromatography by binding in the presence of Ca2+ and elution with a Ca(2+)-chelating agent...
  22. Raghunathan S, Chandross R, Cheng B, Persechini A, Sobottka S, Kretsinger R. The linker of des-Glu84-calmodulin is bent. Proc Natl Acad Sci U S A. 1993;90:6869-73 pubmed
    The crystal structure of a mutant calmodulin (CaM) lacking Glu-84 has been refined to R = 0.23 using data measured to 2.9-A resolution. In native CaM the central helix is fully extended, and the molecule is dumbbell shaped...
  23. Kumrungsee T, Saiki T, Akiyama S, Nakashima K, Tanaka M, Kobayashi Y, et al. Inhibition of calcium-calmodulin complex formation by vasorelaxant basic dipeptides demonstrated by in vitro and in silico analyses. Biochim Biophys Acta. 2014;1840:3073-8 pubmed publisher
    Tryptophan-histidine (Trp-His) was found to suppress the activity of the Ca²?/calmodulin (CaM)-dependent protein kinases II (CaMKII), which requires the Ca²?-CaM complex for an initial activation...
  24. Baekgaard L, Luoni L, De Michelis M, Palmgren M. The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains. J Biol Chem. 2006;281:1058-65 pubmed
    ..Binding of calmodulin (CaM) to this region results in pump activation but the structural basis for CaM activation is still not clear...
  25. Song Y, Zweier J, Xia Y. Heat-shock protein 90 augments neuronal nitric oxide synthase activity by enhancing Ca2+/calmodulin binding. Biochem J. 2001;355:357-60 pubmed
    ..nNOS activity is primarily initiated and regulated by the binding of Ca(2+)/calmodulin (CaM). Therefore, we explored whether hsp90 modulates nNOS activity by affecting CaM binding...
  26. Dolze E, Chigri F, Höwing T, Hierl G, Isono E, Vothknecht U, et al. Calmodulin-like protein AtCML3 mediates dimerization of peroxisomal processing protease AtDEG15 and contributes to normal peroxisome metabolism. Plant Mol Biol. 2013;83:607-24 pubmed publisher
    ..We describe the Ca(2+)/calmodulin (CaM) mediated dimerization of AtDEG15...
  27. Bazari W, Clarke M. Characterization of a novel calmodulin from Dictyostelium discoideum. J Biol Chem. 1981;256:3598-603 pubmed
    We have purified calmodulin from the eukaryotic microorganism Dictyostelium discoideum (Clarke, M., Bazari, W. L., and Kayman, S. C. (1980) J. Bacteriol. 141, 397-400) and have compared it to calmodulin purified from bovine brain...
  28. Liao W, Elfrink K, Bähler M. Head of myosin IX binds calmodulin and moves processively toward the plus-end of actin filaments. J Biol Chem. 2010;285:24933-42 pubmed publisher
    ..We identified in the head of class IX myosins a calmodulin-binding site at the N terminus of loop 2 that is unique among the myosin superfamily members...
  29. Kang S, Kwon H, Wen H, Song Y, Frueh D, Ahn H, et al. Global dynamic conformational changes in the suppressor domain of IP3 receptor by stepwise binding of the two lobes of calmodulin. FASEB J. 2011;25:840-50 pubmed publisher
    The roles of calmodulin (CaM) have been key points of controversy in the regulation of inositol-1,4,5-trisphosphate receptor (IP(3)R)...
  30. Li H, Sánchez Torres J, Del Carpio A, Salas V, Villalobo A. The ErbB2/Neu/HER2 receptor is a new calmodulin-binding protein. Biochem J. 2004;381:257-66 pubmed
    We have demonstrated previously that the EGFR (epidermal growth factor receptor) is a calmodulin (CaM)-binding protein...
  31. Kaur P, Kiselar J, Yang S, Chance M. Quantitative protein topography analysis and high-resolution structure prediction using hydroxyl radical labeling and tandem-ion mass spectrometry (MS). Mol Cell Proteomics. 2015;14:1159-68 pubmed publisher
    ..We tested the methods by examination of Ca(+2)-calmodulin showing highly significant correlations between surface area and side-chain contact predictions for individual ..
  32. Tapp H, Al Naggar I, Yarmola E, Harrison A, Shaw G, Edison A, et al. MARCKS is a natively unfolded protein with an inaccessible actin-binding site: evidence for long-range intramolecular interactions. J Biol Chem. 2005;280:9946-56 pubmed
    ..Intact MARCKS also does not bind as well to calmodulin as does the PSD alone...
  33. Volkmann N, Martásek P, Roman L, Xu X, Page C, Swift M, et al. Holoenzyme structures of endothelial nitric oxide synthase - an allosteric role for calmodulin in pivoting the FMN domain for electron transfer. J Struct Biol. 2014;188:46-54 pubmed publisher
    ..eNOS) holoenzyme produced detailed models of the intact holoenzyme in the presence and absence of Ca(2+)/calmodulin (CaM)...
  34. Bauer M, O Connell D, Cahill D, Linse S. Calmodulin binding to the polybasic C-termini of STIM proteins involved in store-operated calcium entry. Biochemistry. 2008;47:6089-91 pubmed publisher
    ..We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of STIM1 and STIM2, a region critical for their ..
  35. Contessi S, Haraux F, Mavelli I, Lippe G. Identification of a conserved calmodulin-binding motif in the sequence of F0F1 ATPsynthase inhibitor protein. J Bioenerg Biomembr. 2005;37:317-26 pubmed
    ..We characterized the interaction of CaM with purified bovine IF1, two bovine IF1 synthetic peptides, as well as two homologous proteins from yeast, namely ..
  36. Laub M, Steppuhn J, Blüggel M, Immler D, Meyer H, Jennissen H. Modulation of calmodulin function by ubiquitin-calmodulin ligase and identification of the responsible ubiquitylation site in vertebrate calmodulin. Eur J Biochem. 1998;255:422-31 pubmed
    b>Calmodulin is the universal calcium modulator in eukaryotic cells. Its biological activity is closely regulated by the second messenger Ca2+. Previous studies in cell-free extracts [Laub, M. & Jennissen, H. P. (1997) Biochim. Biophys...
  37. Finn B, Drakenberg T, Forsen S. The structure of apo-calmodulin. A 1H NMR examination of the carboxy-terminal domain. FEBS Lett. 1993;336:368-74 pubmed
    The structure of the carboxy-terminal domain of bovine calmodulin, TR2C, in the calcium-free form was investigated using two-dimensional 1H NMR. Sequential resonance assignments were made using standard methods...
  38. Cook W, Walter L, Walter M. Drug binding by calmodulin: crystal structure of a calmodulin-trifluoperazine complex. Biochemistry. 1994;33:15259-65 pubmed
    The crystal structure of calmodulin (CaM) bound to trifluoperazine (TFP) has been determined and refined to a resolution of 2.45 A...
  39. Shen X, Valencia C, Gao W, Cotten S, Dong B, Huang B, et al. Ca(2+)/Calmodulin-binding proteins from the C. elegans proteome. Cell Calcium. 2008;43:444-56 pubmed
    b>Calmodulin (CaM) is the primary Ca(2+)-sensor that regulates a wide variety of cellular processes in eukaryotes...
  40. Martin Nieto J, Villalobo A. The human epidermal growth factor receptor contains a juxtamembrane calmodulin-binding site. Biochemistry. 1998;37:227-36 pubmed
    A ligand-insensitive form of the human epidermal growth factor receptor (EGFR) was enriched by Ca2+-dependent calmodulin-affinity chromatography purification...
  41. Meador W, Means A, Quiocho F. Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex. Science. 1992;257:1251-5 pubmed
    The crystal structure of calcium-bound calmodulin (Ca(2+)-CaM) bound to a peptide analog of the CaM-binding region of chicken smooth muscle myosin light chain kinase has been determined and refined to a resolution of 2.4 angstroms (A)...