Gene Symbol: cdt1.L
Description: chromatin licensing and DNA replication factor 1 L homeolog
Alias: cdt1, dup, ris2, xcdt1, DNA replication factor Cdt1
Species: common platanna

Top Publications

  1. Arias E, Walter J. PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication. Nat Cell Biol. 2006;8:84-90 pubmed
    Ubiquitin-mediated proteolysis of the replication licensing factor Cdt1 (Cdc10-dependent transcript 1) in S phase is a key mechanism that limits DNA replication to a single round per cell cycle in metazoans...
  2. Maiorano D, Rul W, Mechali M. Cell cycle regulation of the licensing activity of Cdt1 in Xenopus laevis. Exp Cell Res. 2004;295:138-49 pubmed
    b>Cdt1 is a conserved replication factor required in licensing the chromosome for a single round of DNA synthesis. The activity of Cdt1 is inhibited by geminin. The mechanism by which geminin interferes with Cdt1 activity is unknown...
  3. Tsuyama T, Watanabe S, Aoki A, Cho Y, Seki M, Enomoto T, et al. Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts. Mol Biol Cell. 2009;20:937-47 pubmed publisher
    Excess Cdt1 reportedly induces rereplication of chromatin in cultured cells and Xenopus egg extracts, suggesting that the regulation of Cdt1 activity by cell cycle-dependent proteolysis and expression of the Cdt1 inhibitor geminin is ..
  4. You Z, Ode K, Shindo M, Takisawa H, Masai H. Characterization of conserved arginine residues on Cdt1 that affect licensing activity and interaction with Geminin or Mcm complex. Cell Cycle. 2016;15:1213-26 pubmed publisher
    All organisms ensure once and only once replication during S phase through a process called replication licensing. Cdt1 is a key component and crucial loading factor of Mcm complex, which is a central component for the eukaryotic ..
  5. Lutzmann M, Maiorano D, Mechali M. A Cdt1-geminin complex licenses chromatin for DNA replication and prevents rereplication during S phase in Xenopus. EMBO J. 2006;25:5764-74 pubmed
    Initiation of DNA synthesis involves the loading of the MCM2-7 helicase onto chromatin by Cdt1 (origin licensing). Geminin is thought to prevent relicensing by binding and inhibiting Cdt1...
  6. Narasimhachar Y, Coue M. Geminin stabilizes Cdt1 during meiosis in Xenopus oocytes. J Biol Chem. 2009;284:27235-42 pubmed publisher
    ..As a promoter, Geminin stabilizes Cdt1 and facilitates its accumulation leading to the assembly of the pre-replication complex on DNA...
  7. Nakazaki Y, Tsuyama T, Seki M, Takahashi M, Enomoto T, Tada S. Excess Cdt1 inhibits nascent strand elongation by repressing the progression of replication forks in Xenopus egg extracts. Biochem Biophys Res Commun. 2016;470:405-410 pubmed publisher
    b>Cdt1 is a protein essential for initiation of DNA replication; it recruits MCM helicase, a core component of the replicative DNA helicase, onto replication origins...
  8. Maiorano D, Krasinska L, Lutzmann M, Mechali M. Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts. Curr Biol. 2005;15:146-53 pubmed
    ..b>Cdt1, a factor binding to origins and recruiting the MCM2-7 helicase, has been identified as a component of the ..
  9. Maiorano D, Moreau J, Mechali M. XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. Nature. 2000;404:622-5 pubmed
    ..This factor (XCDT1) is a relative of fission yeast cdt1, a protein proposed to function in DNA replication, and is the first to be ..

More Information


  1. Franz A, Orth M, Pirson P, Sonneville R, Blow J, Gartner A, et al. CDC-48/p97 coordinates CDT-1 degradation with GINS chromatin dissociation to ensure faithful DNA replication. Mol Cell. 2011;44:85-96 pubmed publisher
    ..Moreover, our data confirmed a similar regulation in Xenopus egg extracts, emphasizing a conserved coordination of licensing and elongation events during eukaryotic DNA replication by CDC-48/p97. ..
  2. Raman M, Havens C, Walter J, Harper J. A genome-wide screen identifies p97 as an essential regulator of DNA damage-dependent CDT1 destruction. Mol Cell. 2011;44:72-84 pubmed publisher
    Several proteins, including the replication licensing factor CDT1 and the histone methyltransferase SET8, are targeted for proteolysis during DNA replication and repair by the E3 ubiquitin ligase CRL4(CDT2)...
  3. Lutzmann M, Mechali M. MCM9 binds Cdt1 and is required for the assembly of prereplication complexes. Mol Cell. 2008;31:190-200 pubmed publisher
    ..We show that MCM9 forms a stable complex with the licensing factor Cdt1, preventing an excess of geminin on chromatin during the licensing reaction...
  4. Iizuka M, Matsui T, Takisawa H, Smith M. Regulation of replication licensing by acetyltransferase Hbo1. Mol Cell Biol. 2006;26:1098-108 pubmed
    ..which involves the sequential binding to replication origins of the origin recognition complex (ORC), Cdc6/Cdc18, Cdt1, and the minichromosome maintenance complex (Mcm2-Mcm7, or Mcm2-7)...
  5. Kisielewska J, Blow J. Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos. Development. 2012;139:63-74 pubmed publisher
    b>Cdt1 plays a key role in licensing DNA for replication. In the somatic cells of metazoans, both Cdt1 and its natural inhibitor geminin show reciprocal fluctuations in their protein levels owing to cell cycle-dependent proteolysis...
  6. Li A, Blow J. Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus. EMBO J. 2005;24:395-404 pubmed
    ..Three additional factors--the origin recognition complex, Cdc6 and Cdt1--are required for origin licensing. We examine here how licensing is regulated in Xenopus egg extracts...
  7. . Don't blame NASA alone for Mars mission failures. Nature. 2000;404:527 pubmed
  8. Nakazaki Y, Tsuyama T, Azuma Y, Takahashi M, Tada S. Mutant analysis of Cdt1's function in suppressing nascent strand elongation during DNA replication in Xenopus egg extracts. Biochem Biophys Res Commun. 2017;490:1375-1380 pubmed publisher
    ..In higher eukaryotes, activity of the Cdt1 protein is temporally regulated during the cell cycle, and deregulation of Cdt1 induces DNA re-replication...
  9. Havens C, Walter J. Docking of a specialized PIP Box onto chromatin-bound PCNA creates a degron for the ubiquitin ligase CRL4Cdt2. Mol Cell. 2009;35:93-104 pubmed publisher
    Substrates of the E3 ubiquitin ligase CRL4(Cdt2), including Cdt1 and p21, contain a PCNA-binding motif called a PIP box. Upon binding of the PIP box to PCNA on chromatin, CRL4(Cdt2) is recruited and the substrate is ubiquitylated...