Gene Symbol: cdk1.S
Description: cyclin-dependent kinase 1 S homeolog
Alias: MPF, PSTAIR, cdc-2, cdc2, cdc2-a, cdc28a, cdc2x1.1, cdk1-a, cdk1-b, p34cdc2, xcdc2, cyclin-dependent kinase 1-A, Cell division control protein 2 homolog 1, cell division control protein 2-A, cell division cycle 2 like, cell division protein kinase 1-A, maturation-promoting factor, p34 protein kinase 1
Species: common platanna
Products:     cdk1.S

Top Publications

  1. El Dika M, Dudka D, Prigent C, Tassan J, Kloc M, Kubiak J. Control of timing of embryonic M-phase entry and exit is differentially sensitive to CDK1 and PP2A balance. Int J Dev Biol. 2014;58:767-74 pubmed publisher
    ..We thus show, for the first time, that equilibrium between CDK1 and PP2A specifies the timing of M-phase entry and exit and regulates the dynamics of cyclin B degradation upon M-phase exit in Xenopus laevis first embryonic mitosis. ..
  2. Poon R, Yamashita K, Adamczewski J, Hunt T, Shuttleworth J. The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2. EMBO J. 1993;12:3123-32 pubmed
    Activation of the cyclin-dependent protein kinases p34cdc2 and p33cdk2 requires binding with a cyclin partner and phosphorylation on the first threonine residue in the sequence THEVVTLWYRAPE...
  3. Romanowski P, Marr J, Madine M, Rowles A, Blow J, Gautier J, et al. Interaction of Xenopus Cdc2 x cyclin A1 with the origin recognition complex. J Biol Chem. 2000;275:4239-43 pubmed
    ..kinase activity also copurifies with ORC over several fractionation steps and was identified as a complex of the Cdc2 catalytic subunit and cyclin A1. Neither Cdk2 nor cyclin E could be detected in ORC immunoprecipitations...
  4. Roig J, Mikhailov A, Belham C, Avruch J. Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression. Genes Dev. 2002;16:1640-58 pubmed
    ..Nercc1 is a cytoplasmic protein that is activated during mitosis and is avidly phosphorylated by active p34(Cdc2)...
  5. Borysov S, Guadagno T. A novel role for Cdk1/cyclin B in regulating B-raf activation at mitosis. Mol Biol Cell. 2008;19:2907-15 pubmed publisher
    ..Taken together, our data reveal a novel cell cycle mechanism for activating the B-Raf/MEK/MAPK cascade. ..
  6. Chesnel F, Bazile F, Pascal A, Kubiak J. Cyclin B dissociation from CDK1 precedes its degradation upon MPF inactivation in mitotic extracts of Xenopus laevis embryos. Cell Cycle. 2006;5:1687-98 pubmed
    Cyclin B is a regulatory subunit of CDK1 within MPF complex. Degradation of cyclin B via ubiquitin-proteasome pathway seemed to be absolutely required for the M-phase exit...
  7. Daldello E, Le T, Poulhe R, Jessus C, Haccard O, Dupré A. Control of Cdc6 accumulation by Cdk1 and MAPK is essential for completion of oocyte meiotic divisions in Xenopus. J Cell Sci. 2015;128:2482-96 pubmed publisher
  8. Huang T, Pian J, PAN B. Oncogenic Ras suppresses Cdk1 in a complex manner during the incubation of activated Xenopus egg extracts. Arch Biochem Biophys. 2013;532:61-72 pubmed publisher
    ..Taken together, these results indicate that oncogenic Ras suppresses Cdk1 in a complex manner: It induces continuous accumulation of cyclin B2, but also causes persistent inhibitory phosphorylation of tyr-15-Cdk1. ..
  9. Peterson S, Li Y, Chait B, Gottesman M, Baer R, Gautier J. Cdk1 uncouples CtIP-dependent resection and Rad51 filament formation during M-phase double-strand break repair. J Cell Biol. 2011;194:705-20 pubmed publisher
    ..We have thus identified Cdk1 as a critical regulator of DSB repair in M phase. Cdk1 induces persistent ssDNA-RPA overhangs in M phase, thereby preventing both classical NHEJ and Rad51-dependent HDR...

More Information


  1. Setoyama D, Yamashita M, Sagata N. Mechanism of degradation of CPEB during Xenopus oocyte maturation. Proc Natl Acad Sci U S A. 2007;104:18001-6 pubmed
    ..During Xenopus oocyte maturation, CPEB undergoes a Cdc2-mediated phosphorylation- and ubiquitin-dependent degradation that is required for proper entry into meiosis II...
  2. D Angiolella V, Palazzo L, Santarpia C, Costanzo V, Grieco D. Role for non-proteolytic control of M-phase-promoting factor activity at M-phase exit. PLoS ONE. 2007;2:e247 pubmed
    M-phase Promoting Factor (MPF; the cyclin B-cdk 1 complex) is activated at M-phase onset by removal of inhibitory phosphorylation of cdk1 at thr-14 and tyr-15. At M-phase exit, MPF is destroyed by ubiquitin-dependent cyclin proteolysis...
  3. Inoue D, Sagata N. The Polo-like kinase Plx1 interacts with and inhibits Myt1 after fertilization of Xenopus eggs. EMBO J. 2005;24:1057-67 pubmed
    ..cycle in Xenopus oocytes, p90(rsk), the downstream kinase of the Mos-MAPK pathway, interacts with and inhibits the Cdc2 inhibitory kinase Myt1. However, p90(rsk) is inactivated after fertilization due to the degradation of Mos...
  4. Cheng A, Xiong W, Ferrell J, Solomon M. Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins. Cell Cycle. 2005;4:155-65 pubmed
    ..Of the mammalian proteins, two, Speedy/Ringo A and C, bind to Cdc2 and Cdk2, whereas Speedy/Ringo B binds preferentially to Cdc2...
  5. Wu Q, Guo Y, Yamada A, Perry J, Wang M, Araki M, et al. A role for Cdc2- and PP2A-mediated regulation of Emi2 in the maintenance of CSF arrest. Curr Biol. 2007;17:213-24 pubmed here the molecular details of a negative feedback loop wherein Cyclin B promotes its own destruction through Cdc2/Cyclin B-mediated phosphorylation and inhibition of the APC inhibitor Emi2...
  6. Losada A, Yokochi T, Kobayashi R, Hirano T. Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes. J Cell Biol. 2000;150:405-16 pubmed
    ..Purified cdc2-cyclin B can phosphorylate XSA1 in vitro and reduce the ability of x-cohesin(SA1) to bind to DNA or chromatin...
  7. Lin C, Huang Y, Richter J. Transient CPEB dimerization and translational control. RNA. 2012;18:1050-61 pubmed publisher
    ..In addition, the dimers might serve as molecular hubs that release polyadenylation factors for translational activation upon CPEB dimer destruction...
  8. Gravells P, Tomita K, Booth A, Poznansky J, Porter A. Chemical genetic analyses of quantitative changes in Cdk1 activity during the human cell cycle. Hum Mol Genet. 2013;22:2842-51 pubmed publisher
    ..Furthermore, the viability of cells that are unable to undergo rapid Cdk1 activation, and the strong association between endoreduplication and impaired proliferation, may place restrictions on the therapeutic use of a Cdk1 inhibitors. ..
  9. El Dika M, Laskowska Kaszub K, Koryto M, Dudka D, Prigent C, Tassan J, et al. CDC6 controls dynamics of the first embryonic M-phase entry and progression via CDK1 inhibition. Dev Biol. 2014;396:67-80 pubmed publisher
    ..Thus, CDK1 activation proceeds with concomitant inhibition by CDC6, which tunes the timing of the M-phase entry during the embryonic cell cycle. ..
  10. Fesquet D, Labbe J, Derancourt J, Capony J, Galas S, Girard F, et al. The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues. EMBO J. 1993;12:3111-21 pubmed
    Phosphorylation of Thr161, a residue conserved in all members of the cdc2 family, has been reported to be absolutely required for the catalytic activity of cdc2, the major regulator of eukaryotic cell cycle...
  11. Haccard O, Jessus C. Redundant pathways for Cdc2 activation in Xenopus oocyte: either cyclin B or Mos synthesis. EMBO Rep. 2006;7:321-5 pubmed
    ..induces the resumption of meiotic maturation, which requires continuous protein synthesis to bring about Cdc2 activation. The identification of the newly synthesized proteins has long been a goal...
  12. Pickham K, Meyer A, Li J, Donoghue D. Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites. Mol Cell Biol. 1992;12:3192-203 pubmed
    The p34cdc2 protein kinase is a component of maturation-promoting factor, the master regulator of the cell cycle in all eukaryotes. The activity of p34cdc2 is itself tightly regulated by phosphorylation and dephosphorylation...
  13. Nakamura N, Tokumoto T, Ueno S, Iwao Y. The cytoskeleton-dependent localization of cdc2/cyclin B in blastomere cortex during Xenopus embryonic cell cycle. Mol Reprod Dev. 2005;72:336-45 pubmed
    ..At the 8th cell cycle, most of the cdc2/cyclin B was localized in the cortical cytoplasm throughout the cell cycle, in the centrosomes and the nucleus at ..
  14. Freedman B, Heald R. Functional comparison of H1 histones in Xenopus reveals isoform-specific regulation by Cdk1 and RanGTP. Curr Biol. 2010;20:1048-52 pubmed publisher
    ..Our results reveal distinct regulatory mechanisms among linker histone isoforms and a specific role for H1M to compact chromosomes during egg meiotic arrest and early embryonic divisions. ..
  15. Ferby I, Blazquez M, Palmer A, Eritja R, Nebreda A. A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes. Genes Dev. 1999;13:2177-89 pubmed
    ..We also show that p33(ringo) binds to and activates the kinase activity of p34(cdc2) but does not associate with p34(cdc2)/cyclin B complexes...
  16. Mayr P, Allan V, Woodman P. Phosphorylation of p97(VCP) and p47 in vitro by p34cdc2 kinase. Eur J Cell Biol. 1999;78:224-32 pubmed
    ..p97/Cdc48p contains two conserved consensus p34cdc2 kinase phosphorylation sites within its second ATP binding domain...