Gene Symbol: HYD1
Description: iron hydrogenase
Species: Chlamydomonas reinhardtii

Top Publications

  1. Godman J, Molnar A, Baulcombe D, Balk J. RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii. Biochem J. 2010;431:345-51 pubmed publisher
    ..Together, our results clearly distinguish the cellular roles of HydA1 and HYD3, and indicate that HYD3, like its yeast and human homologues, has an evolutionary conserved role in the biogenesis or maintenance of cytosolic Fe-S proteins. ..
  2. Senger M, Mebs S, Duan J, Wittkamp F, Apfel U, Heberle J, et al. Stepwise isotope editing of [FeFe]-hydrogenases exposes cofactor dynamics. Proc Natl Acad Sci U S A. 2016;113:8454-9 pubmed publisher
    ..Our experimental protocol for site-selective (13)CO isotope editing combined with computational species assignment opens new perspectives for characterization of functional intermediates in the catalytic cycle. ..
  3. Liran O, Semyatich R, Milrad Y, Eilenberg H, Weiner I, Yacoby I. Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016;172:264-71 pubmed publisher
    ..In summary, we show that an anaerobic locality in the chloroplast preserves [FeFe]-hydrogenase activity and supports continuous hydrogen production in air-grown microalgal cells. ..
  4. Silakov A, Kamp C, Reijerse E, Happe T, Lubitz W. Spectroelectrochemical characterization of the active site of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii. Biochemistry. 2009;48:7780-6 pubmed publisher
    ..The removal of the bridging CO moiety has been observed in the H(red) to H(sred) transition. The significance of this result for the hydrogen conversion mechanism of this class of enzymes is discussed...
  5. Czech I, Stripp S, Sanganas O, Leidel N, Happe T, Haumann M. The [FeFe]-hydrogenase maturation protein HydF contains a H-cluster like [4Fe4S]-2Fe site. FEBS Lett. 2011;585:225-30 pubmed publisher
    ..Presumably, only the 2Fe cluster is transferred from HydF to HydA1, thereby forming the active site...
  6. Meuser J, D Adamo S, Jinkerson R, Mus F, Yang W, Ghirardi M, et al. Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H? production. Biochem Biophys Res Commun. 2012;417:704-9 pubmed publisher
    ..Interestingly, enhanced in vitro and fermentative H(2) production activities were observed in the hydA1-1 hydA2-1 strain complemented with HYDA1, while maximal H(2)-photoproduction rates did not exceed those of wild-type cells...
  7. Ghysels B, Godaux D, Matagne R, Cardol P, Franck F. Function of the chloroplast hydrogenase in the microalga Chlamydomonas: the role of hydrogenase and state transitions during photosynthetic activation in anaerobiosis. PLoS ONE. 2013;8:e64161 pubmed publisher
    ..These two processes are thus important for survival of the cells when they are transiently placed in an anaerobic environment. ..
  8. Rumpel S, Siebel J, Diallo M, Farès C, Reijerse E, Lubitz W. Structural Insight into the Complex of Ferredoxin and [FeFe] Hydrogenase from Chlamydomonas reinhardtii. Chembiochem. 2015;16:1663-9 pubmed publisher
    ..This provides valuable information about residues crucial for complex formation and electron transfer. The derived complex model might help to pinpoint residue substitution targets for improved hydrogen production. ..
  9. Stripp S, Sanganas O, Happe T, Haumann M. The structure of the active site H-cluster of [FeFe] hydrogenase from the green alga Chlamydomonas reinhardtii studied by X-ray absorption spectroscopy. Biochemistry. 2009;48:5042-9 pubmed publisher
    ..g., to the binding of an exogenous CO at 2Fe(H) in CO-inhibited enzyme. Our XAS studies pave the way for the monitoring of atomic level structural changes at the H-cluster during H(2) catalysis...

More Information


  1. Pape M, Lambertz C, Happe T, Hemschemeier A. Differential expression of the Chlamydomonas [FeFe]-hydrogenase-encoding HYDA1 gene is regulated by the copper response regulator1. Plant Physiol. 2012;159:1700-12 pubmed publisher
    ..Electrophoretic mobility shift assays showed that the CRR1 SBP domain binds to one of the GTAC cores in vitro. These combined results prove that CRR1 is involved in HYDA1 promoter activation...
  2. Swirsky Whitney L, Novi G, Perata P, Loreti E. Distinct mechanisms regulating gene expression coexist within the fermentative pathways in Chlamydomonas reinhardtii. ScientificWorldJournal. 2012;2012:565047 pubmed publisher
    ..green algae Chlamydomonas reinhardtii may produce hydrogen by means of its hydrogenase enzymes, in particular HYD1, using reductants derived from the degradation of intercellular carbon stores...
  3. Gilbert Wilson R, Siebel J, Adamska Venkatesh A, Pham C, Reijerse E, Wang H, et al. Spectroscopic Investigations of [FeFe] Hydrogenase Maturated with [(57)Fe2(adt)(CN)2(CO)4](2-). J Am Chem Soc. 2015;137:8998-9005 pubmed publisher
    ..The Hox-CO state of [2(57)Fe]H and [4(57)Fe-4S]H HydA1 was characterized by Mössbauer, HYSCORE, ENDOR, and nuclear resonance vibrational spectroscopy. ..