Scap

Summary

Gene Symbol: Scap
Description: SREBF chaperone
Alias: sterol regulatory element-binding protein cleavage-activating protein, SREBP cleavage-activating protein
Species: Chinese hamster

Top Publications

  1. Sun L, Li L, Goldstein J, Brown M. Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro. J Biol Chem. 2005;280:26483-90 pubmed publisher
    ..or 25-hydroxycholesterol to microsomal membranes in vitro blocks Sar1-dependent binding of the Sec23/24 complex to Scap, the SREBP escort protein...
  2. Radhakrishnan A, Sun L, Kwon H, Brown M, Goldstein J. Direct binding of cholesterol to the purified membrane region of SCAP: mechanism for a sterol-sensing domain. Mol Cell. 2004;15:259-68 pubmed
    ..Transport is blocked by cholesterol, which triggers SCAP, the SREBP escort protein, to bind to Insigs, which are ER retention proteins...
  3. Nohturfft A, DeBose Boyd R, Scheek S, Goldstein J, Brown M. Sterols regulate cycling of SREBP cleavage-activating protein (SCAP) between endoplasmic reticulum and Golgi. Proc Natl Acad Sci U S A. 1999;96:11235-40 pubmed
    ..cleavage of sterol regulatory element-binding proteins (SREBPs) is regulated by SREBP cleavage-activating protein (SCAP), which forms complexes with SREBPs in membranes of the endoplasmic reticulum (ER)...
  4. Hua X, Nohturfft A, Goldstein J, Brown M. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell. 1996;87:415-26 pubmed
    Through expression cloning we have isolated a cDNA-encoding SREBP cleavage-activating protein (SCAP), which regulates cholesterol metabolism by stimulating cleavage of transcription factors SREBP-1 and -2, thereby releasing them from ..
  5. Sakai J, Nohturfft A, Goldstein J, Brown M. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem. 1998;273:5785-93 pubmed
    ..cleavage, apparently by interacting with a polytopic membrane protein designated SREBP cleavage-activating protein (SCAP)...
  6. Nohturfft A, Brown M, Goldstein J. Topology of SREBP cleavage-activating protein, a polytopic membrane protein with a sterol-sensing domain. J Biol Chem. 1998;273:17243-50 pubmed
    ..from endoplasmic reticulum membranes by proteases whose activities depend upon SREBP cleavage-activating protein (SCAP), a polytopic endoplasmic reticulum membrane protein...
  7. Rawson R, DeBose Boyd R, Goldstein J, Brown M. Failure to cleave sterol regulatory element-binding proteins (SREBPs) causes cholesterol auxotrophy in Chinese hamster ovary cells with genetic absence of SREBP cleavage-activating protein. J Biol Chem. 1999;274:28549-56 pubmed
    ..proteins (SREBPs) at site 1, due to mutations in the gene encoding SREBP cleavage-activating protein (SCAP)...
  8. Yang T, Goldstein J, Brown M. Overexpression of membrane domain of SCAP prevents sterols from inhibiting SCAP.SREBP exit from endoplasmic reticulum. J Biol Chem. 2000;275:29881-6 pubmed
    b>SCAP (SREBP cleavage-activating protein) forms a complex with sterol regulatory element-binding proteins (SREBPs) and escorts them from the endoplasmic reticulum (ER) to the Golgi complex where proteases release transcriptionally active ..
  9. Espenshade P, Li W, Yabe D. Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER. Proc Natl Acad Sci U S A. 2002;99:11694-9 pubmed publisher
    ..reticulum-to-Golgi transport of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP), a sterol-sensing protein that escorts SREBPs...

More Information

Publications14

  1. Yabe D, Xia Z, Adams C, Rawson R. Three mutations in sterol-sensing domain of SCAP block interaction with insig and render SREBP cleavage insensitive to sterols. Proc Natl Acad Sci U S A. 2002;99:16672-7 pubmed publisher
    ..Sequence analysis of SREBP cleavage-activating protein (SCAP) transcripts from SRD-5 cells revealed the presence of a mutation in one SCAP allele that results in substitution ..
  2. Feramisco J, Radhakrishnan A, Ikeda Y, Reitz J, Brown M, Goldstein J. Intramembrane aspartic acid in SCAP protein governs cholesterol-induced conformational change. Proc Natl Acad Sci U S A. 2005;102:3242-7 pubmed publisher
    The polytopic membrane protein SCAP transports sterol regulatory element-binding proteins (SREBPs) from the endoplasmic reticulum (ER) to the Golgi, thereby activating cholesterol synthesis...
  3. Sun L, Seemann J, Goldstein J, Brown M. Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi: Insig renders sorting signal in Scap inaccessible to COPII proteins. Proc Natl Acad Sci U S A. 2007;104:6519-26 pubmed publisher
    ..ER) to the Golgi by preventing the binding of COPII-coated proteins to a hexapeptide sorting signal (MELADL) in Scap, the SREBP-escort protein...
  4. Huber M, Vesely P, Datta K, Gerace L. Erlins restrict SREBP activation in the ER and regulate cellular cholesterol homeostasis. J Cell Biol. 2013;203:427-36 pubmed publisher
    Cellular cholesterol levels are controlled by endoplasmic reticulum (ER) sterol sensing proteins, which include Scap and Insig-1...
  5. Zhang Y, Lee K, Kinch L, Clark L, Grishin N, Rosenbaum D, et al. Direct Demonstration That Loop1 of Scap Binds to Loop7: A CRUCIAL EVENT IN CHOLESTEROL HOMEOSTASIS. J Biol Chem. 2016;291:12888-96 pubmed publisher
    Cholesterol homeostasis is mediated by Scap, a polytopic endoplasmic reticulum (ER) protein that transports sterol regulatory element-binding proteins from the ER to Golgi, where they are processed to forms that activate cholesterol ..