connexin 45 6


Gene Symbol: connexin 45 6
Description: gap junction protein alpha 8
Alias: Cx45.6, Cx50, connexin-45.6, gap junction alpha-8 protein, connexin 45.6, connexin 50
Species: chicken
Products:     connexin 45 6

Top Publications

  1. Yin X, Gu S, Jiang J. The development-associated cleavage of lens connexin 45.6 by caspase-3-like protease is regulated by casein kinase II-mediated phosphorylation. J Biol Chem. 2001;276:34567-72 pubmed
    ..Finally, caspase-3-mediated cleavage can be regulated by casein kinase II-mediated phosphorylation, suggesting that Cx45.6 turnover and specific cleavage by caspase-3-like protease is alternatively modulated. ..
  2. Yu X, Jiang J. Interaction of major intrinsic protein (aquaporin-0) with fiber connexins in lens development. J Cell Sci. 2004;117:871-80 pubmed
  3. Yu X, Yin X, Lafer E, Jiang J. Developmental regulation of the direct interaction between the intracellular loop of connexin 45.6 and the C terminus of major intrinsic protein (aquaporin-0). J Biol Chem. 2005;280:22081-90 pubmed
    ..Finally this interaction had no impact on Cx45.6-mediated intercellular communication, suggesting that the Cx45.6-MIP(AQP0) interaction plays a novel unidentified role in lens fibers. ..
  4. Yin X, Jedrzejewski P, Jiang J. Casein kinase II phosphorylates lens connexin 45.6 and is involved in its degradation. J Biol Chem. 2000;275:6850-6 pubmed
    Connexin (Cx) 45.6, an avian counterpart of rodent Cx50, is phosphorylated in vivo, but the sites and function of the phosphorylation have not been elucidated...
  5. Yin X, Liu J, Jiang J. Lens fiber connexin turnover and caspase-3-mediated cleavage are regulated alternately by phosphorylation. Cell Commun Adhes. 2008;15:1-11 pubmed publisher
    ..6 by caspase-3. These findings provide further insights into regulatory mechanism of the specific phosphorylation of connexins in the lens...
  6. Liu J, Xu J, Gu S, Nicholson B, Jiang J. Aquaporin 0 enhances gap junction coupling via its cell adhesion function and interaction with connexin 50. J Cell Sci. 2011;124:198-206 pubmed publisher
    Both connexin 50 (Cx50) and aquaporin 0 (AQP0) have important roles in lens development and homeostasis, and their mutations are associated with human congenital cataracts. We have previously shown that Cx50 directly interacts with AQP0...
  7. Jiang J, White T, Goodenough D, Paul D. Molecular cloning and functional characterization of chick lens fiber connexin 45.6. Mol Biol Cell. 1994;5:363-73 pubmed
    ..6 and ChCx56 were abundant in lens fibers. Treatment of lens membranes with alkaline phosphatase resulted in electrophoretic mobility shifts, demonstrating that both ChCx45.6 and ChCx56 were phosphoproteins in vivo. ..
  8. Sawada K, Agata K, Eguchi G. Characterization of terminally differentiated cell state by categorizing cDNA clones derived from chicken lens fibers. Int J Dev Biol. 1996;40:531-5 pubmed
  9. Banks E, Toloue M, Shi Q, Zhou Z, Liu J, Nicholson B, et al. Connexin mutation that causes dominant congenital cataracts inhibits gap junctions, but not hemichannels, in a dominant negative manner. J Cell Sci. 2009;122:378-88 pubmed publisher
    ..When expressed in paired Xenopus oocytes, human (h) and chicken (ch) Cx50 E48K mutants showed no electrical coupling...

More Information


  1. Shi Q, Banks E, Yu X, Gu S, Lauer J, Fields G, et al. Amino acid residue Val362 plays a critical role in maintaining the structure of C terminus of connexin 50 and in lens epithelial-fiber differentiation. J Biol Chem. 2010;285:18415-22 pubmed publisher
    ..Here, we show that deletion of the PEST motif at the C terminus (CT) domain of Cx50 attenuates the stimulatory effect of Cx50 on lens fiber differentiation...
  2. Pinto B, GarcĂ­a I, Pupo A, Retamal M, Martinez A, Latorre R, et al. Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins. J Biol Chem. 2016;291:15740-52 pubmed publisher
    ..Here, we determined the role of the charged residues at the end of TM-1 in voltage sensing in Cx26, Cx46, and Cx50. Conductance/voltage curves obtained from tail currents together with kinetics analysis reveal that the fast and ..
  3. Banks E, Yu X, Shi Q, Jiang J. Promotion of lens epithelial-fiber differentiation by the C-terminus of connexin 45.6 a role independent of gap junction communication. J Cell Sci. 2007;120:3602-12 pubmed
    ..Here, we took advantage of naturally occurring loss-of-gap-junction function mutations of Cx50 (ortholog of chick Cx45.6) and generated the corresponding site mutants in Cx45.6: Cx45.6(D47A) and Cx45.6(P88S)...
  4. Dong L, Liu X, Li H, Vertel B, Ebihara L. Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels. J Physiol. 2006;576:787-99 pubmed
    Previous studies have shown that gap junctional channels formed from the lens connexins Cx50 (or its chicken orthologue, Cx45.6) and Cx43 exhibit marked differences in transjunctional voltage gating and unitary conductance...
  5. Liu J, Ek Vitorin J, Weintraub S, Gu S, Shi Q, Burt J, et al. Phosphorylation of connexin 50 by protein kinase A enhances gap junction and hemichannel function. J Biol Chem. 2011;286:16914-28 pubmed publisher
    ..Here, we showed by mass spectrometry that Ser-395 in the C terminus of chicken Cx50 was phosphorylated in the lens. Ser-395 is located within a PKA consensus site...
  6. Wang K, Gu S, Yin X, Weintraub S, Hua Z, Jiang J. Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect lens cells against ultraviolet radiation. J Biol Chem. 2012;287:15786-97 pubmed publisher
    ..We have shown previously that Cx50 is likely to be cleaved by caspase-3 like protease during chick lens development...