Gene Symbol: CENPT
Description: centromere protein T
Alias: CENP-T, centromere protein T
Amano M, Suzuki A, Hori T, Backer C, Okawa K, Cheeseman I, et al
. The CENP-S complex is essential for the stable assembly of outer kinetochore structure. J Cell Biol. 2009;186:173-82 pubmed publisher
..In addition, we found that intrakinetochore distance was increased in CENP-S- and CENP-X-deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore. ..
Johnston K, Joglekar A, Hori T, Suzuki A, Fukagawa T, Salmon E. Vertebrate kinetochore protein architecture: protein copy number. J Cell Biol. 2010;189:937-43 pubmed publisher
Nishino T, Takeuchi K, Gascoigne K, Suzuki A, Hori T, Oyama T, et al
. CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold. Cell. 2012;148:487-501 pubmed publisher
..These data suggest that the CENP-T-W-S-X complex forms a unique nucleosome-like structure to generate contacts with DNA, extending the "histone code" beyond canonical nucleosome proteins...
Hori T, Amano M, Suzuki A, Backer C, Welburn J, Dong Y, et al
. CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. Cell. 2008;135:1039-52 pubmed publisher
..In total, our results suggest that the CENP-T/CENP-W complex is directly involved in establishment of centromere chromatin structure coordinately with CENP-A. ..
Suzuki A, Hori T, Nishino T, Usukura J, Miyagi A, Morikawa K, et al
. Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins. J Cell Biol. 2011;193:125-40 pubmed publisher
Gascoigne K, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman I. Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes. Cell. 2011;145:410-22 pubmed publisher
..We additionally find that CENP-T phosphorylation regulates the mitotic assembly of both endogenous and ectopic kinetochores. Thus, CENP-C and CENP-T form a critical regulated platform for vertebrate kinetochore assembly. ..
Ribeiro S, Vagnarelli P, Dong Y, Hori T, McEwen B, Fukagawa T, et al
. A super-resolution map of the vertebrate kinetochore. Proc Natl Acad Sci U S A. 2010;107:10484-9 pubmed publisher
..In mitosis, a CENP-C-dependent mechanism crosslinks CENP-A blocks of different layers together, conferring extra stability to the kinetochore. ..
Hori T, Shang W, Takeuchi K, Fukagawa T. The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly. J Cell Biol. 2013;200:45-60 pubmed publisher
..Based on the analysis of these different engineered kinetochores, we conclude that the CCAN has two distinct roles: recruiting CENP-A to establish the kinetochore and serving as a structural core to directly recruit kinetochore proteins...
Wood L, Booth D, Vargiu G, Ohta S, deLima Alves F, Samejima K, et al
. Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability. Open Biol. 2016;6:150230 pubmed publisher
..This study highlights the different roles core kinetochore components may play in the assembly of kinetochores (upon entry into mitosis) versus the maintenance of specific components (during mitosis). ..