chbF

Summary

Gene Symbol: chbF
Description: phospho-chitobiase; general 6-phospho-beta-glucosidase activity
Alias: ECK1732, JW1723, celF, ydjD
Species:

Top Publications

  1. ncbi The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla 92093 0116
    Res Microbiol 141:1061-7. 1990
  2. ncbi Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12
    L L Parker
    Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269
    Genetics 124:455-71. 1990
  3. ncbi Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12
    M Kricker
    Genetics 115:419-29. 1987
  4. ncbi Directed evolution of cellobiose utilization in Escherichia coli K12
    M Kricker
    University of Connecticut, Storrs 06268
    Mol Biol Evol 1:171-82. 1984
  5. ncbi A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel
    A Guzzo
    McGill University, Department of Microbiology and Immunology, Montreal, Quebec, Canada
    Mol Gen Genet 242:455-60. 1994
  6. ncbi Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon
    N O Keyhani
    Department of Biology and The McCollum Pratt Institute, The Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 94:14367-71. 1997
  7. ncbi Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: characterization and assignment to the unusual family 4 of glycosylhydrolases
    J Thompson
    Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892, USA
    J Bacteriol 181:7339-45. 1999
  8. ncbi Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose
    Aashiq H Kachroo
    Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India
    Mol Microbiol 66:1382-95. 2007
  9. ncbi The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system
    N O Keyhani
    Department of Biology and The McCollum Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 275:33084-90. 2000

Scientific Experts

  • John Thompson
  • N O Keyhani
  • Aashiq H Kachroo
  • S Roseman
  • Nongmaithem S Singh
  • Aswani K Kancherla
  • Subramony Mahadevan
  • Umesh Varshney
  • Y C Lee
  • L X Wang
  • A Guzzo
  • M Kricker
  • M S DuBow
  • B G Hall
  • L L Parker
  • J Reizer
  • M H Saier
  • A Reizer

Detail Information

Publications9

  1. ncbi The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus
    J Reizer
    Department of Biology, University of California at San Diego, La Jolla 92093 0116
    Res Microbiol 141:1061-7. 1990
    ..The cellobiose permease is the only one of several homologous sequenced permeases of the phosphoenolpyruvate:sugar phosphotransferase system which has its three known functional domains residing on distinct polypeptide chains...
  2. ncbi Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12
    L L Parker
    Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269
    Genetics 124:455-71. 1990
    ..the transport and phosphorylation of beta-glucoside sugars; celD, which encodes a negative regulatory protein; and celF, which encodes a phospho-beta-glucosidase that acts on phosphorylated cellobiose, arbutin and salicin...
  3. ncbi Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12
    M Kricker
    Genetics 115:419-29. 1987
    ..Other strains inducibly express a gene which specifies transport of arbutin but not the other beta-glucosides. The arbutin transport gene, arbT, maps outside of the cel locus...
  4. ncbi Directed evolution of cellobiose utilization in Escherichia coli K12
    M Kricker
    University of Connecticut, Storrs 06268
    Mol Biol Evol 1:171-82. 1984
    ..A fourth gene at an unknown location increases the growth rate on cellobiose. The cel genes constitute a second cryptic system for beta-glucoside utilization in E. coli K12...
  5. ncbi A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel
    A Guzzo
    McGill University, Department of Microbiology and Immunology, Montreal, Quebec, Canada
    Mol Gen Genet 242:455-60. 1994
    ..A molecular analysis revealed that the insertion occurred within the celF gene of E. coli...
  6. ncbi Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon
    N O Keyhani
    Department of Biology and The McCollum Pratt Institute, The Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 94:14367-71. 1997
    ..of the gene products as follows: celA to chbB, celB to chbC, celC to chbA, celD to chbR, and celF to chbF. Furthermore, sequencing evidence indicates that the operon contains an additional gene of unknown function to be ..
  7. ncbi Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: characterization and assignment to the unusual family 4 of glycosylhydrolases
    J Thompson
    Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892, USA
    J Bacteriol 181:7339-45. 1999
    The gene celF of the cryptic cel operon of Escherichia coli has been cloned, and the encoded 6-phospho-beta-glucosidase (cellobiose-6-phosphate [6P] hydrolase; CelF [EC 3.2.1...
  8. ncbi Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose
    Aashiq H Kachroo
    Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India
    Mol Microbiol 66:1382-95. 2007
    ....
  9. ncbi The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system
    N O Keyhani
    Department of Biology and The McCollum Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 275:33084-90. 2000
    ..e. glucose and GlcNAc. Presumably the PTS sugars inhibit by competing for PTS components. Kinetic properties of the transport system are described...