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Genomes and GenesSpecies | CEG1SummaryGene Symbol: CEG1 Description: Ceg1p Species: Top Publications
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Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicingL D Fresco
Harvard Medical School, Boston, Massachusetts 02115, USA
RNA 2:584-96. 1996..in intact yeast cells, conditional alleles of the gene encoding the capping enzyme guanylyltransferase subunit (CEG1) were generated...- The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivoY Takase
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 20:9307-16. 2000..mRNA capping enzyme consists of two subunits: an RNA 5'-triphosphatase (Cet1) and an mRNA guanylyltransferase (Ceg1)... - An essential function of Saccharomyces cerevisiae RNA triphosphatase Cet1 is to stabilize RNA guanylyltransferase Ceg1 against thermal inactivationS Hausmann
Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
J Biol Chem 276:36116-24. 2001Saccharomyces cerevisiae RNA triphosphatase (Cet1) and RNA guanylyltransferase (Ceg1) interact in vivo and in vitro to form a bifunctional mRNA capping enzyme complex... - The yeast capping enzyme represses RNA polymerase II transcriptionLawrence C Myers
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
Mol Cell 10:883-94. 2002..Efficient pre-mRNA capping relies on interactions between the capping enzyme and transcription apparatus. Repression by the capping enzyme suggests a bidirectional flow of information between capping and transcription... - Divergent subunit interactions among fungal mRNA 5'-capping machineriesToshimitsu Takagi
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 021151, USA
Eukaryot Cell 1:448-57. 2002..The GTase subunit (Ceg1) binds to the phosphorylated carboxyl-terminal domain of the largest subunit (CTD-P) of RNA polymerase II (pol II), .. - mRNA capping enzyme activity is coupled to an early transcription elongationHye Jin Kim
Department of Biochemistry and Molecular Biology, College of Pharmacy, Sungkyunkwan University, Suwon, Kyonggi do 440 746, South Korea
Mol Cell Biol 24:6184-93. 2004One of the temperature-sensitive alleles of CEG1, a guanylyltransferase subunit of the Saccharomyces cerevisiae capping enzyme, showed 6-azauracil (6AU) sensitivity at the permissive growth temperature, which is a phenotype that is .. - Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear exportFrancisco Estruch
Departamento de Bioquimica y Biologia Molecular, Facultad de Biologia, Universitat de Valencia, Dr Moliner, 50 Burjassot 46100, Spain
J Biol Chem 280:9691-7. 2005..We proposed that Zds1p associates with the complex formed by Dbp5p, Gfd1p, and nucleoporins at the cytosolic fibrils of the nuclear pore complex and is required for optimal mRNA export... - The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcriptionOlga Calvo
Department of Biological Sciences, Columbia University, New York, NY 10027, USA
EMBO J 24:1009-20. 2005..Our data provide evidence that Rna15 and Sub1 are present along the length of several genes and that Sub1 facilitates elongation by influencing enzymes that modify RNAP II... - Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatusMeigang Gu
Structural Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
Structure 18:216-27. 2010..cerevisiae, the first two steps in capping are catalyzed by the RNA triphosphatase Cet1 and RNA guanylyltransferase Ceg1, which form a complex that is directly recruited to phosphorylated RNA polymerase II (RNAP IIo), primarily via .. - The yeast 5'-3' exonuclease Rat1p functions during transcription elongation by RNA polymerase IISilvia Jimeno-González
Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, Aarhus DK 8000, Denmark
Mol Cell 37:580-7. 2010..Consistently, Rat1p-dependent RNAPII termination is also observed in the capping-deficient ceg1-63 strain... - A dual interface determines the recognition of RNA polymerase II by RNA capping enzymeMan Hee Suh
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
J Biol Chem 285:34027-38. 2010..Our results indicate that the dual interface based on combining PCI1 and PCI2 is required for directing CE to Pol II elongation complexes... - Dynamic association of capping enzymes with transcribing RNA polymerase IIS C Schroeder
Department of Biochemistry and Molecular Genetics, UCHSC, Denver, Colorado 80262, USA
Genes Dev 14:2435-40. 2000..In vitro, the yeast mRNA capping enzymes Ceg1 and Abd1 bind specifically to the phosphorylated CTD... - Inhibition of mRNA turnover in yeast by an xrn1 mutation enhances the requirement for eIF4E binding to eIF4G and for proper capping of transcripts by Ceg1pJ T Brown
Section of Molecular Genetics and Microbiology and the Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712 1095, USA
Genetics 155:31-42. 2000..Another allele of CDC33, cdc33-1, along with mutations in CEG1, encoding the nuclear guanylyltransferase, were also synthetic lethal with xrn1Delta, whereas mutations in PRT1, .. - Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase IIC R Rodriguez
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 20:104-12. 2000..Immunoblotting suggests that the capping enzyme guanylyltransferase (Ceg1) is stabilized in vivo by its interaction with the CTD and that serine 5, the major site of phosphorylation within .. - Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzymeB Schwer
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
Gene Expr 5:331-44. 1996..CES2, and CES3-- that, when present in high copy, suppress the ts growth defect caused by mutations in the CEG1 gene encoding mRNA guanylyltransferase (capping enzyme)... - Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viabilityT Tsukamoto
Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
Biochem Biophys Res Commun 239:116-22. 1997..We have isolated the alpha subunit gene (CEG1) by immunological screening... - 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase IIS McCracken
Amgen Institute and Ontario Cancer Institute, Toronto, Ontario M5G 2C1, Canada
Genes Dev 11:3306-18. 1997..These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation... - mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domainE J Cho
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Genes Dev 11:3319-26. 1997..Our results provide in vitro and in vivo evidence that capping enzyme is recruited to the transcription complex via phosphorylation of the RNA polymerase CTD... - Effects of deletion mutations in the yeast Ces1 protein on cell growth and morphology and on high copy suppression of mutations in mRNA capping enzyme and translation initiation factor 4AB Schwer
Department of Microbiology, Cornell University Medical College, New York, NY 10021, USA
Nucleic Acids Res 26:803-9. 1998..Delta ces 4 cold-sensitive phenotype, to restore normal morphology and to suppress the temparature-sensitive mutant ceg1-25 ... - Accelerated mRNA decay in conditional mutants of yeast mRNA capping enzymeB Schwer
Microbiology Department, Cornell University Medical College, New York and Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
Nucleic Acids Res 26:2050-7. 1998..Here, we analyzed gene expression in Saccharomyces cerevisiae cells bearing conditional mutations of Ceg1 (capping enzyme), a 52 kDa protein that transfers GMP from GTP to the 5' end of mRNA to form the GpppN cap .. - Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatusC K Ho
Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
Mol Cell Biol 18:5189-98. 1998..gene, CES5, that when present in high copy, suppresses the temperature-sensitive growth defect caused by the ceg1-25 mutation of the yeast mRNA guanylyltransferase (capping enzyme)... - Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domainE J Cho
Harvard Medical School, Boston, Massachusetts 02115 USA
Genes Dev 12:3482-7. 1998..capping enzyme is composed of two subunits, the mRNA 5'-triphosphatase (Cet1) and the mRNA guanylyltransferase (Ceg1). Here we map interactions between Ceg1, Cet1, and the CTD... - A conserved domain of yeast RNA triphosphatase flanking the catalytic core regulates self-association and interaction with the guanylyltransferase component of the mRNA capping apparatusK Lehman
Molecular Biology Program, Sloan Kettering Institute, New York, NY, USA
J Biol Chem 274:22668-78. 1999.... - An essential surface motif (WAQKW) of yeast RNA triphosphatase mediates formation of the mRNA capping enzyme complex with RNA guanylyltransferaseC K Ho
Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
Nucleic Acids Res 27:4671-8. 1999..Trypsin-sensitive sites of Ceg1p that are shielded from proteolysis when Ceg1p is bound to Cet1p are located between nucleotidyl transferase motifs V and VI... - The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongationM Carmen Garcia-Lopez
Departamento de Biologia Experimental, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071 Jaen, Spain
Genetics 189:1235-48. 2011..analyses point to a role for these proteins in transcriptional initiation and/or early elongation, consistent with their genetic interactions with CEG1, a guanylyltransferase subunit of the Saccharomyces cerevisiae capping enzyme.