Gene Symbol: unc-60
Description: Actin-depolymerizing factor 1;Actin-depolymerizing factor 2
Alias: Actin-depolymerizing factor 1;Actin-depolymerizing factor 2
Species: Caenorhabditis elegans
Products:     unc-60

Top Publications

  1. Ono S, Baillie D, Benian G. UNC-60B, an ADF/cofilin family protein, is required for proper assembly of actin into myofibrils in Caenorhabditis elegans body wall muscle. J Cell Biol. 1999;145:491-502 pubmed
    ..We conclude that precise control of actin filament dynamics by UNC-60B is required for proper integration of actin into myofibrils. ..
  2. Ono K, Yamashiro S, Ono S. Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad. J Cell Sci. 2008;121:2662-70 pubmed publisher
    ..Our results suggest that an optimal level of actin-filament-severing activity of ADF/cofilin is required for assembly of actin networks in the somatic gonad. ..
  3. McKim K, Matheson C, Marra M, Wakarchuk M, Baillie D. The Caenorhabditis elegans unc-60 gene encodes proteins homologous to a family of actin-binding proteins. Mol Gen Genet. 1994;242:346-57 pubmed
    ..elegans muscle. unc-60 has an essential function in development, since one unc-60 allele, s1586, has a recessive lethal phenotype. Our characterization of s1586 has shown that it is a small deletion which disrupts both coding regions. ..
  4. Ono K, Parast M, Alberico C, Benian G, Ono S. Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans. J Cell Sci. 2003;116:2073-85 pubmed
    ..These results suggest that the ADF/cofilin isoforms play distinct roles in specific aspects of actin reorganization in vivo. ..
  5. Johnsen R, Baillie D. Genetic analysis of a major segment [LGV(left)] of the genome of Caenorhabditis elegans. Genetics. 1991;129:735-52 pubmed
    ..The analysis of essential genes in large regions should help to define C. elegans in terms of all its genes and aid in the understanding of the relationship of genome structure to genome function. ..
  6. Ohno G, Ono K, Togo M, Watanabe Y, Ono S, Hagiwara M, et al. Muscle-specific splicing factors ASD-2 and SUP-12 cooperatively switch alternative pre-mRNA processing patterns of the ADF/cofilin gene in Caenorhabditis elegans. PLoS Genet. 2012;8:e1002991 pubmed publisher
  7. Pavlov D, Muhlrad A, Cooper J, Wear M, Reisler E. Actin filament severing by cofilin. J Mol Biol. 2007;365:1350-8 pubmed
    ..These conclusions have particular relevance to cofilin function during actin-based motility in cells and in synthetic systems. ..
  8. Yamashiro S, Gimona M, Ono S. UNC-87, a calponin-related protein in C. elegans, antagonizes ADF/cofilin-mediated actin filament dynamics. J Cell Sci. 2007;120:3022-33 pubmed
    ..Our results demonstrate that actin binding via calponin-like repeats competes with ADF/cofilin-driven cytoskeletal turnover, and is critical for providing the spatiotemporal regulation of actin filament stability. ..
  9. Tian D, Diao M, Jiang Y, Sun L, Zhang Y, Chen Z, et al. Anillin Regulates Neuronal Migration and Neurite Growth by Linking RhoG to the Actin Cytoskeleton. Curr Biol. 2015;25:1135-45 pubmed publisher
    ..Our results reveal a novel pathway in which Anillin transduces the RhoG signal to the actin cytoskeleton during neuronal migration and neurite growth. ..

More Information


  1. Alder M, Dames S, Gaudet J, Mango S. Gene silencing in Caenorhabditis elegans by transitive RNA interference. RNA. 2003;9:25-32 pubmed
    ..These data suggest dsRNA synthesized in vivo can mediate systemic RNAi. ..
  2. Shen Q, He B, Lu N, Conradt B, Grant B, Zhou Z. Phagocytic receptor signaling regulates clathrin and epsin-mediated cytoskeletal remodeling during apoptotic cell engulfment in C. elegans. Development. 2013;140:3230-43 pubmed publisher
    ..Our work reveals novel roles of clathrin and epsin in apoptotic-cell internalization, suggests a Hip1/R-independent mechanism linking clathrin to actin assembly, and ties the CED-1 pathway to cytoskeleton remodeling. ..
  3. Hagedorn E, Kelley L, Naegeli K, Wang Z, Chi Q, Sherwood D. ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo. J Cell Biol. 2014;204:1209-18 pubmed publisher
    ..Together, these results identify the invadopodial membrane as a specialized compartment whose recycling to form dynamic, functional invadopodia is dependent on localized F-actin disassembly by ADF/cofilin...
  4. Ono S. Purification and biochemical characterization of actin from Caenorhabditis elegans: its difference from rabbit muscle actin in the interaction with nematode ADF/cofilin. Cell Motil Cytoskeleton. 1999;43:128-36 pubmed
    ..The results indicate that C. elegans actin shares common biochemical properties with rabbit muscle actin, while actin-binding proteins can interact with C. elegans actin in a distinct manner from rabbit muscle actin. ..
  5. Ono S, Ono K. Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics. J Cell Biol. 2002;156:1065-76 pubmed
    ..However, in an ADF/cofilin mutant background, suppression of CeTM did not worsen actin organization and worm motility. These results suggest that tropomyosin is a physiological inhibitor of ADF/cofilin-dependent actin dynamics. ..
  6. Nukazuka A, Fujisawa H, Inada T, Oda Y, Takagi S. Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2alpha in Caenorhabditis elegans. Genes Dev. 2008;22:1025-36 pubmed publisher
    ..Thus, our results reveal a physiological significance for translation of mRNAs for cytoskeletal regulators, linking environmental cues to cytoskeletal rearrangement during cellular morphogenesis in vivo. ..
  7. Yamashiro S, Cox E, Baillie D, Hardin J, Ono S. Sarcomeric actin organization is synergistically promoted by tropomodulin, ADF/cofilin, AIP1 and profilin in C. elegans. J Cell Sci. 2008;121:3867-77 pubmed publisher
    ..Thus, we propose a model in which tropomodulin and enhancers of actin dynamics synergistically regulate elongation and shortening of actin filaments at the pointed end. ..