Gene Symbol: spd-2
Description: Spindle-defective protein 2
Alias: Spindle-defective protein 2
Species: Caenorhabditis elegans

Top Publications

  1. Sugioka K, Hamill D, Lowry J, McNeely M, Enrick M, Richter A, et al. Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation. elife. 2017;6: pubmed publisher
    ..We conclude that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important roles in the orchestration of centriole and PCM assembly. ..
  2. Stubenvoll M, Medley J, Irwin M, Song M. ATX-2, the C. elegans Ortholog of Human Ataxin-2, Regulates Centrosome Size and Microtubule Dynamics. PLoS Genet. 2016;12:e1006370 pubmed publisher
    ..Our data suggest that RNA-binding proteins play an active role in controlling MT dynamics and provide insight into the control of proper centrosome size and MT dynamics. ..
  3. Srayko M, Kaya A, Stamford J, Hyman A. Identification and characterization of factors required for microtubule growth and nucleation in the early C. elegans embryo. Dev Cell. 2005;9:223-36 pubmed
    ..However, we found that centrosomes do not nucleate MTs to saturation; rather, the depolymerizing kinesin-13 subfamily member KLP-7 is required to limit microtubule outgrowth from centrosomes. ..
  4. Delattre M, Canard C, GONCZY P. Sequential protein recruitment in C. elegans centriole formation. Curr Biol. 2006;16:1844-9 pubmed
    ..Several of these proteins have homologs in other metazoans, and we expect the assembly pathway that stems from our work to be conserved. ..
  5. Yang R, Feldman J. SPD-2/CEP192 and CDK Are Limiting for Microtubule-Organizing Center Function at the Centrosome. Curr Biol. 2015;25:1924-31 pubmed publisher
    ..We show that conversion of MTOC state involves the conserved centrosome protein SPD-2/CEP192 and CDK activity from the mitotic cell. ..
  6. Shimanovskaya E, Viscardi V, Lesigang J, Lettman M, Qiao R, Svergun D, et al. Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases. Structure. 2014;22:1090-1104 pubmed publisher
    ..Overall, our findings suggest a conserved mechanism for centriolar docking of Plk4 homologs that initiate daughter centriole assembly. ..
  7. Kemp C, Kopish K, Zipperlen P, Ahringer J, O Connell K. Centrosome maturation and duplication in C. elegans require the coiled-coil protein SPD-2. Dev Cell. 2004;6:511-23 pubmed
    ..Thus, we have identified SPD-2 as a factor critical for the two basic functions of the centrosome-microtubule organization and duplication. ..
  8. O Rourke S, Yochem J, Connolly A, Price M, Carter L, Lowry J, et al. Rapid mapping and identification of mutations in Caenorhabditis elegans by restriction site-associated DNA mapping and genomic interval pull-down sequencing. Genetics. 2011;189:767-78 pubmed publisher
    ..Together, these two methods provide a rapid and cost-effective approach for positional cloning of C. elegans mutant loci, and are also applicable to other genetic model systems. ..
  9. Decker M, Jaensch S, Pozniakovsky A, Zinke A, O Connell K, Zachariae W, et al. Limiting amounts of centrosome material set centrosome size in C. elegans embryos. Curr Biol. 2011;21:1259-67 pubmed publisher
    ..This idea could be a general mechanism for setting the size of intracellular organelles during development. ..

More Information


  1. Kemp C, Song M, Addepalli M, Hunter G, O CONNELL K. Suppressors of zyg-1 define regulators of centrosome duplication and nuclear association in Caenorhabditis elegans. Genetics. 2007;176:95-113 pubmed
    ..Our approach has thus identified numerous candidate regulators of centrosome duplication and uncovered an unanticipated regulatory mechanism involving factors that tether the centrosome to the nucleus. ..
  2. Cowan C, Hyman A. Cyclin E-Cdk2 temporally regulates centrosome assembly and establishment of polarity in Caenorhabditis elegans embryos. Nat Cell Biol. 2006;8:1441-7 pubmed
    ..elegans and Drosophila. Here, we suggest a possible mechanism for cyclin E-Cdk2-dependent differentiation: the establishment of cortical polarity by the centrosome. ..
  3. O Connell K, Leys C, White J. A genetic screen for temperature-sensitive cell-division mutants of Caenorhabditis elegans. Genetics. 1998;149:1303-21 pubmed
    ..We conclude that this approach can be used to identify mutations that affect various aspects of the cell-division cycle. ..
  4. Song M, Liu Y, Anderson D, Jahng W, O Connell K. Protein phosphatase 2A-SUR-6/B55 regulates centriole duplication in C. elegans by controlling the levels of centriole assembly factors. Dev Cell. 2011;20:563-71 pubmed publisher
    ..Together, our findings indicate that PP2A-SUR-6 promotes centriole assembly by protecting ZYG-1 and SAS-5 from degradation. ..
  5. Schvarzstein M, Pattabiraman D, BEMBENEK J, Villeneuve A. Meiotic HORMA domain proteins prevent untimely centriole disengagement during Caenorhabditis elegans spermatocyte meiosis. Proc Natl Acad Sci U S A. 2013;110:E898-907 pubmed publisher
    ..elegans also function to inhibit centriole separation at meiosis II, thereby ensuring that the zygote inherits the appropriate complement of chromosomes and centrioles...
  6. Pelletier L, O TOOLE E, Schwager A, Hyman A, MULLER REICHERT T. Centriole assembly in Caenorhabditis elegans. Nature. 2006;444:619-23 pubmed
    ..These results define a structural pathway for the assembly of a daughter centriole and should have general relevance for future studies on centriole assembly in other organisms. ..
  7. Woodruff J, Wueseke O, Viscardi V, Mahamid J, Ochoa S, Bunkenborg J, et al. Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Science. 2015;348:808-12 pubmed publisher
    ..Thus, PCM size and binding capacity emerge from the regulated polymerization of one coiled-coil protein to form a porous network. ..
  8. Pelletier L, Ozlu N, Hannak E, Cowan C, Habermann B, Ruer M, et al. The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication. Curr Biol. 2004;14:863-73 pubmed
    ..elegans. SPD-2 shares homology with a human centrosome protein, suggesting that this key component of the C. elegans centrosome is evolutionarily conserved. ..