Gene Symbol: rrf-3
Description: RNA-dependent RNA polymerase Family
Alias: RNA-dependent RNA polymerase Family
Species: Caenorhabditis elegans
Products:     rrf-3

Top Publications

  1. Gent J, Schvarzstein M, Villeneuve A, Gu S, Jantsch V, Fire A, et al. A Caenorhabditis elegans RNA-directed RNA polymerase in sperm development and endogenous RNA interference. Genetics. 2009;183:1297-314 pubmed publisher
    ..These and other data suggest a working model in which a major role of the RRF-3/ERI pathway is to generate siRNAs that set patterns of gene expression through feedback repression of a set of critical targets during spermatogenesis. ..
  2. Simmer F, Tijsterman M, Parrish S, Koushika S, Nonet M, Fire A, et al. Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr Biol. 2002;12:1317-9 pubmed
    ..The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens. ..
  3. Gent J, Lamm A, Pavelec D, Maniar J, Parameswaran P, Tao L, et al. Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma. Mol Cell. 2010;37:679-89 pubmed publisher
    ..The requirement for two RdRP/Argonaute combinations and initiation by a rare class of uniquely structured siRNAs in this pathway illustrate the caution and flexibility used as biological systems exploit the physiological copying of RNA. ..
  4. Asikainen S, Storvik M, Lakso M, Wong G. Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants. FEBS Lett. 2007;581:5050-4 pubmed
    ..These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes. ..
  5. Zhuang J, Hunter C. Tissue specificity of Caenorhabditis elegans enhanced RNA interference mutants. Genetics. 2011;188:235-7 pubmed publisher
    ..Enhanced RNAi (Eri) mutants facilitate RNAi due to their hypersensitivity to dsRNA. Here, we compare eight Eri mutants for sensitivity to ingested dsRNA, targeting a variety of tissue-specific genes. ..
  6. Thivierge C, Makil N, Flamand M, Vasale J, Mello C, Wohlschlegel J, et al. Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi. Nat Struct Mol Biol. 2011;19:90-7 pubmed publisher
    ..Our results implicate tudor proteins in the recruitment of RdRP complexes to specific steps within DCR-1-dependent and DCR-1-independent endo-RNAi pathways. ..
  7. Lockwood C, Zaidel Bar R, Hardin J. The C. elegans zonula occludens ortholog cooperates with the cadherin complex to recruit actin during morphogenesis. Curr Biol. 2008;18:1333-7 pubmed publisher
    ..These results show that ZOO-1 cooperates with the cadherin-catenin complex to dynamically regulate strong junctional anchorage to the actin cytoskeleton during morphogenesis. ..
  8. Spanier B, Lasch K, Marsch S, Benner J, Liao W, Hu H, et al. How the intestinal peptide transporter PEPT-1 contributes to an obesity phenotype in Caenorhabditits elegans. PLoS ONE. 2009;4:e6279 pubmed publisher
  9. Pavelec D, Lachowiec J, Duchaine T, Smith H, Kennedy S. Requirement for the ERI/DICER complex in endogenous RNA interference and sperm development in Caenorhabditis elegans. Genetics. 2009;183:1283-95 pubmed publisher

More Information


  1. Gu S, Pak J, Guang S, Maniar J, Kennedy S, Fire A. Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint. Nat Genet. 2012;44:157-64 pubmed publisher
    ..These results implicate dsRNA-triggered chromatin modification in C. elegans as a programmable and locus-specific response defining a metastable state that can persist through generational boundaries. ..