Gene Symbol: rpm-1
Description: E3 ubiquitin-protein ligase rpm-1
Alias: E3 ubiquitin-protein ligase rpm-1
Species: Caenorhabditis elegans

Top Publications

  1. Baker S, Opperman K, Tulgren E, Turgeon S, Bienvenut W, Grill B. RPM-1 uses both ubiquitin ligase and phosphatase-based mechanisms to regulate DLK-1 during neuronal development. PLoS Genet. 2014;10:e1004297 pubmed publisher
    ..Thus, PHR proteins are potentially more accurate and sensitive regulators of DLK than originally thought. Our results also highlight an important and expanding role for the PP2C phosphatase family in neuronal development. ..
  2. Yan D, Wu Z, Chisholm A, Jin Y. The DLK-1 kinase promotes mRNA stability and local translation in C. elegans synapses and axon regeneration. Cell. 2009;138:1005-18 pubmed publisher
    ..Our findings identify the DLK-1 pathway as a regulator of mRNA stability in synapse formation and maintenance and also in adult axon regeneration. ..
  3. Hammarlund M, Nix P, Hauth L, Jorgensen E, Bastiani M. Axon regeneration requires a conserved MAP kinase pathway. Science. 2009;323:802-6 pubmed publisher
    ..We conclude that after axon injury, activation of this MAP kinase cascade is required to switch the mature neuron from an aplastic state to a state capable of growth. ..
  4. Nakata K, Abrams B, Grill B, Goncharov A, Huang X, Chisholm A, et al. Regulation of a DLK-1 and p38 MAP kinase pathway by the ubiquitin ligase RPM-1 is required for presynaptic development. Cell. 2005;120:407-20 pubmed
    ..DLK-1 protein levels are elevated in rpm-1 mutants. The RPM-1 RING finger can stimulate ubiquitination of DLK-1. Our data reveal a presynaptic role of a previously unknown p38 MAP kinase cascade. ..
  5. Abrams B, Grill B, Huang X, Jin Y. Cellular and molecular determinants targeting the Caenorhabditis elegans PHR protein RPM-1 to perisynaptic regions. Dev Dyn. 2008;237:630-9 pubmed publisher
    ..We show that activated DLK-1 may be preferentially targeted for degradation. Furthermore, using transgene analysis, we identified a critical role of the conserved PHR domain of RPM-1 in its subcellular localization. ..
  6. Nix P, Hisamoto N, Matsumoto K, Bastiani M. Axon regeneration requires coordinate activation of p38 and JNK MAPK pathways. Proc Natl Acad Sci U S A. 2011;108:10738-43 pubmed publisher
    ..These two MAPKs are coregulated by the E3 ubiquitin ligase RPM-1(Phr1) via targeted degradation of the MAPKKKs DLK-1 and MLK-1 and by the MAPK phosphatase VHP-1(MKP7), which negatively regulates both PMK-3(p38) and KGB-1(JNK). ..
  7. Schaefer A, Hadwiger G, Nonet M. rpm-1, a conserved neuronal gene that regulates targeting and synaptogenesis in C. elegans. Neuron. 2000;26:345-56 pubmed
    ..These results indicate that RPM-1 functions cell autonomously during synaptogenesis to regulate neuronal morphology. ..
  8. Hung W, Hwang C, Gao S, Liao E, Chitturi J, Wang Y, et al. Attenuation of insulin signalling contributes to FSN-1-mediated regulation of synapse development. EMBO J. 2013;32:1745-60 pubmed publisher
    ..We propose that FSN-1 may negatively regulate insulin/IGF signalling, in part, through EGL-3-dependent insulin-like ligand processing. ..
  9. Grill B, Chen L, Tulgren E, Baker S, Bienvenut W, Anderson M, et al. RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans. J Neurosci. 2012;32:2628-36 pubmed publisher
    ..Further, we show that RAE-1 colocalizes with RPM-1 in neurons, and that rae-1 functions downstream of rpm-1. These studies establish a novel postmitotic function for rae-1 in neuronal development. ..

More Information


  1. Park E, Glodowski D, Rongo C. The ubiquitin ligase RPM-1 and the p38 MAPK PMK-3 regulate AMPA receptor trafficking. PLoS ONE. 2009;4:e4284 pubmed publisher
    ..Our findings indicate a novel specialized role for RPM-1 and PMK-3/p38 MAPK in regulating the endosomal trafficking of AMPARs at central synapses. ..
  2. Li H, Kulkarni G, Wadsworth W. RPM-1, a Caenorhabditis elegans protein that functions in presynaptic differentiation, negatively regulates axon outgrowth by controlling SAX-3/robo and UNC-5/UNC5 activity. J Neurosci. 2008;28:3595-603 pubmed publisher
    ..Together, the results suggest that RPM-1 regulates axon outgrowth affecting axon guidance and termination by controlling the trafficking of the UNC-5 and SAX-3 receptors to cell membranes. ..
  3. Grill B, Bienvenut W, Brown H, Ackley B, Quadroni M, Jin Y. C. elegans RPM-1 regulates axon termination and synaptogenesis through the Rab GEF GLO-4 and the Rab GTPase GLO-1. Neuron. 2007;55:587-601 pubmed
    ..We find that late endosomes are specifically disorganized at the presynaptic terminals of glo-4 mutants. Our data suggest that RPM-1 positively regulates a Rab GTPase pathway to promote vesicular trafficking via late endosomes. ..
  4. Zhen M, Huang X, Bamber B, Jin Y. Regulation of presynaptic terminal organization by C. elegans RPM-1, a putative guanine nucleotide exchanger with a RING-H2 finger domain. Neuron. 2000;26:331-43 pubmed
    ..The temperature-sensitive period of rpm-1 coincides with the time of synaptogenesis. rpm-1 may regulate the spatial arrangement, or restrict the formation, of presynaptic structures. ..
  5. Gao M, Liao E, Yu B, Wang Y, Zhen M, Derry W. The SCF FSN-1 ubiquitin ligase controls germline apoptosis through CEP-1/p53 in C. elegans. Cell Death Differ. 2008;15:1054-62 pubmed publisher
    ..Our results uncover a novel role for the SCF(FSN-1) E3 ubiquitin ligase in the regulation of cep-1-dependent germ cell apoptosis. ..
  6. Noma K, Goncharov A, Jin Y. Systematic analyses of rpm-1 suppressors reveal roles for ESS-2 in mRNA splicing in Caenorhabditis elegans. Genetics. 2014;198:1101-15 pubmed publisher
    ..Our findings provide the first functional evidence that this family of proteins regulate mRNA splicing in a context-specific manner. ..
  7. Giles A, Opperman K, Rankin C, Grill B. Developmental Function of the PHR Protein RPM-1 Is Required for Learning in Caenorhabditis elegans. G3 (Bethesda). 2015;5:2745-57 pubmed publisher
    ..Overall, our findings suggest that developmental defects in rpm-1 mutants manifest most prominently in behaviors that require protracted or plastic circuit function, such as learning. ..
  8. Baker S, Grill B. Defining Minimal Binding Regions in Regulator of Presynaptic Morphology 1 (RPM-1) Using Caenorhabditis elegans Neurons Reveals Differential Signaling Complexes. J Biol Chem. 2017;292:2519-2530 pubmed publisher
    ..These results indicate that RPM-1 uses different mechanisms to recruit FSN-1 and RAE-1 into independent signaling complexes in neurons. ..
  9. Li C, Hisamoto N, Nix P, Kanao S, Mizuno T, Bastiani M, et al. The growth factor SVH-1 regulates axon regeneration in C. elegans via the JNK MAPK cascade. Nat Neurosci. 2012;15:551-7 pubmed publisher
    ..Thus, SVH-1-SVH-2 signaling via activation of the MAPK pathway acts to coordinate neuron regeneration response after axon injury. ..
  10. Borgen M, Wang D, Grill B. RPM-1 regulates axon termination by affecting growth cone collapse and microtubule stability. Development. 2017;144:4658-4672 pubmed publisher
    ..These findings provide insight into how growth cone collapse is regulated during axon termination in vivo, and suggest that RPM-1 signaling destabilizes microtubules to facilitate growth cone collapse and axon termination. ..
  11. Kulkarni G, Li H, Wadsworth W. CLEC-38, a transmembrane protein with C-type lectin-like domains, negatively regulates UNC-40-mediated axon outgrowth and promotes presynaptic development in Caenorhabditis elegans. J Neurosci. 2008;28:4541-50 pubmed publisher
    ..We suggest CLEC-38 plays a role in promoting synapse assembly and refining axon outgrowth activity. ..
  12. Quevedo C, Kaplan D, Derry W. AKT-1 regulates DNA-damage-induced germline apoptosis in C. elegans. Curr Biol. 2007;17:286-92 pubmed
    ..Finally, we show that AKT-1 regulates apoptosis but not cell-cycle progression downstream of the HUS-1/MRT-2 branch of the DNA damage checkpoint. ..
  13. Opperman K, Grill B. RPM-1 is localized to distinct subcellular compartments and regulates axon length in GABAergic motor neurons. Neural Dev. 2014;9:10 pubmed publisher
    ..Our findings provide further support for the previously proposed model that PHR proteins function to coordinate axon outgrowth and termination with synapse formation. ..
  14. Sharma J, Baker S, Turgeon S, Gurney A, Opperman K, Grill B. Identification of a peptide inhibitor of the RPM-1 · FSN-1 ubiquitin ligase complex. J Biol Chem. 2014;289:34654-66 pubmed publisher
    ..Our results are consistent with the FSN-1 binding domain of RPM-1 recruiting FSN-1 and a target protein, such as DLK-1, whereas the RING-H2 domain of RPM-1 ubiquitinates the target. ..
  15. Tulgren E, Turgeon S, Opperman K, Grill B. The Nesprin family member ANC-1 regulates synapse formation and axon termination by functioning in a pathway with RPM-1 and ?-Catenin. PLoS Genet. 2014;10:e1004481 pubmed publisher
    ..Our study highlights an emerging, broad role for ANC-1 in neuronal development, and unveils a new and unexpected mechanism by which RPM-1 functions. ..